Haris Ahmed Khan

In Salmonella Typhimurium, efflux pump proteins, such as AcrD actively expel drugs and hazardous chemicals from bacterial cells, resulting in treatment failure and the emergence of antibiotic-resistant variants. Focusing on AcrD may lead to the development of novel antimicrobials against multidrug-resistant bacteria. However, challenges persist in...

Staphylococcus aureus is a common bacterium that causes a variety of infections in humans. This microorganism produces several virulence factors, including hemolysins, which contribute to its disease-causing ability. The treatment of S. aureus infections typically involves the use of antibiotics. However, the emergence of antibiotic-resistant strai...

Staphylococcus aureus is a common bacterium that causes a variety of infections in humans. This microorganism produces several virulence factors, including hemolysins, which contribute to its disease-causing ability. The treatment of S. aureus infections typically involves the use of antibiotics. However, the emergence of antibiotic-resistant strai...

Viral infections and associated illnesses account for approximately 3.5 million global fatalities and public health problems. Medicinal plants, with their wide therapeutic range and minimal side effects, have gained limelight particularly in response to growing concerns about drug resistance and sluggish development of antiviral drugs. This study c...

Malaria remains a worldwide health concern, requiring novel ways in medication development to counteract the parasite Plasmodium's developing resistance. In this work, we investigated the herb Zanthoxylum armatum's potential as a malaria treatment. This plant is well-known for its abundance of phytoconstituents. Through molecular docking experiment...

A wide diversity of mycoviruses has been reported from Botrytis species, some with the potential to suppress the pathogenic abilities of this fungus. Considering their importance, this study was devised to find potential hypovirulence-associated mycoviruses found in Botrytis cinerea strains isolated from Pakistani strawberry fields. Here we report...

Objectives The current study was conducted to evaluate the antibacterial potential of leaf and fruit extracts of Zanthoxylum armatum against two pathogenic bacterial isolates, Staphylococcus aureus and Staphylococcus epidermidis. Methods Twelve commercially available antibiotics were tested S. aureus and S. epidermidis by antimicrobial susceptibil...

Coputational designing of a tetravalent mRNA Vaccine against Dengue Virsus serotypes prevalent in Pakistan.

A wide diversity of mycoviruses have been reported from Botrytis species, some with the potential to supress the pathogenic abilities of this fungus. Considering their importance, this study was devised to find potential hypovirulence associated mycoviruses from in Botrytis cinerea strains isolated from Pakistani strawberry fields. Here we report c...

Members of the genus Sclerotinia are notorious plant pathogens with a diverse host range that includes many important crops. A huge number of mycoviruses have been identified in this genus; some of these viruses are reported to have a hypovirulent effect on the fitness of their fungal hosts. These mycoviruses are important to researchers from a bio...

Mycoviruses are natural inhabitants of fungi and have been identified in almost all fungal taxonomic groups. Mycoviruses that infect phytopathogenic fungi are now becoming a hot research area due to their potential for the biocontrol of important plant pathogens. But, before considering a mycovirus for biocontrol, we should be fully aware of the ef...

β-galactosidase (Lactase), an enzyme belonging to the glycoside hydrolase family causing the hydrolysis and trans-glycosylation of β-D-galactosides, has a vital role in dairy industries. The current investigation emphasizes on in-silico identification and comparative analysis of different fungal lactases present in Aspergillus fumigatus, Aspergillu...

Dengue fever, a highly infectious and rapidly spreading vector borne illness, is classified as a Neglected Tropical Disease (NTD) by WHO because they generally afflict the world's poor and historically have not received as much attention as other diseases. DENV NS2B/NS3 protease and NS5 polymerase are regarded as significant prospective therapeutic...

Botrytis cinerea is a phytopathogenic fungus that belongs to the family Sclerotiniaceae in the Ascomycota group. This pathogen infects economically important crops and is responsible for substantial preharvest and postharvest losses each year worldwide. Mycoviruses are commonly associated with Botrytis cinerea isolates. In this work, we report the...

The number of novel mycoviruses is increasing at a high pace due to advancements in sequencing technologies. As a result, an uncountable number of mycoviral sequences are available in public sequence repositories. However, only genomic information is not sufcient to understand the impact of mycoviruses on their host biology. Biological characteri...

Botrytis cinerea is one of the top phytopathogenic fungus which ubiquitously cause grey mold on a variety of horticultural plants. The mechanism of respiration in the fungus occurs within the mitochondria. Mitogenomes serve as a key molecular marker for the investigation of fungal evolutionary patterns. This study aimed at the complete assembly, ch...

Viroids are the smallest known infectious agents that are thought to only infect plants. Here, we reveal that several species of plant pathogenic fungi that were isolated from apple trees infected with apple scar skin viroid (ASSVd) carried ASSVd naturally. This finding indicates the spread of viroids to fungi under natural conditions and further s...

Mycoviruses are widely distributed across the kingdom Fungi, including ascomycetous yeast strains of the class Saccharo-mycetes. Geotrichum candidum is an important fungal pathogen belonging to Saccharomycetes and has a diverse host range. Here, we report the characterization of four new classical totiviruses from two distinct Geotrichum candidum s...

Diplodia seriata in the family Botryosphaeriaceae is a cosmopolitan phytopathogenic fungus and is responsible for causing cankers, fruit rot and leaf spots on economically important plants. In this study, we characterized the virome of a single Pakistani strain (L3) of D. seriata. Several viral-like contig sequences were obtained via a previously c...

Flowering at the proper time is critical for effective sexual reproduction and the subsequent development of seeds and fruits in plants. This entails synchronizing flowering with the right season and the plant's developmental history. The cruciform weed Arabidopsis has shown discrete but connected pathways for sensing the primary seasonal cues of d...

Modern agricultural production is dominated by the use of synthetic chemical pesticides, which account for 95% of the global market share of total pesticide use. However, this over-reliance on synthetic pesticides adversely affects and interferes with the functioning of the ecosystem. Neem (Azadirachta indica), a botanical biopesticide widely known...

The world is facing an alarming threat of environmental pollution (gases and plastic), resources and energy depletion owing to the traditional production of various building and packaging materials. we are proposing a myco-mat which can be employed in building materials, packaging material and can replace the use of Polyurethane foams. It will prov...

The research is oriented to assess the feasibility of fungi as bio-admixture for concrete to repair the cracks autonomously and modify the crack development attribute. The efficiency of selected fungal species, i.e., Rhizopus oryzae and Trichoderma longibrachiatum, was analyzed on inoculation in concrete on direct induction and after being immobili...

Neofusicoccum parvum is an important plant-pathogenic ascomycetous fungus that causes trunk diseases in a variety of plants. A limited number of reports on mycoviruses from this fungus are available. Here, we report the characterization of a novel victorivirus, Neofusicoccum parvum victorivirus 3 (NpVV3). An agarose gel dsRNA profile of a Pakistani...

An extensive screening survey was conducted on Pakistani filamentous fungal isolates for the identification of viral infections. A total of 396 fungal samples were screened, of which 36 isolates were found double-stranded (ds) RNA positive with an overall frequency of 9% when analysed by a classical dsRNA isolation method. One of 36 dsRNA-positive...

A unique capsidless virus with a positive-sense, single-stranded RNA genome (hadakavirus 1, HadV1), a member of the extended picorna-like supergroup, was isolated previously from the phytopathogenic fungus Fusarium oxysporum. Here, we describe the molecular and biological characterisation of a second hadakavirus strain from Fusarium nygamai, which...

Fungi have raised serious problems for human health as invasive fungal pathogens. These fungal infections are the possible reason of increased death and morbidity rates in immune-compromised patients. In this regard, early diagnosis and characterization of the invasive fungal species is of utmost importance. Currently employed fungal identification...