Hans C P Matthijs

Hans C P Matthijs
University of Amsterdam | UVA · Research Group of Aquatic Microbiology

PhD Biochemistry/Photosynthesis/Aquatic Microbiology

About

192
Publications
65,347
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Introduction
A recent interest is to use fundamental science value to furbish projects that support societal needs. A remarkable difference in the pathway of the Mehler reaction between plants and green algae on the one hand, and the Mehler like reaction in cyanobacteria, made us design a method for selective killing of harmful cyanobacteria directly in lakes using homogenously injected highly diluted hydrogen peroxide. Cyanobacteria are killed, green algae flourish after a treatment.
Additional affiliations
January 2000 - March 2016
University of Amsterdam
Position
  • Professor (Associate)
January 2000 - March 2016
University of Amsterdam
Position
  • Professor (Associate)
January 2000 - March 2016
University of Amsterdam
Position
  • Professor (Associate)

Publications

Publications (192)
Article
Full-text available
Oceanographic studies have shown that heterotrophic bacteria can protect marine cyanobacteria against oxidative stress caused by hydrogen peroxide (H2O2). Could a similar interspecific protection play a role in freshwater ecosystems? In a series of laboratory experiments, we demonstrate that freshwater cyanobacteria are sensitive to H2O2 but can be...
Article
Full-text available
Although cyanobacteria absorb blue light, they use it less efficiently for photosynthesis than other colors absorbed by their photosynthetic pigments. A plausible explanation for this enigmatic phenomenon is that blue light is not absorbed by phycobilisomes and, hence, causes an excitation shortage at photosystem II (PSII). This hypothesis is suppo...
Article
Full-text available
The ubiquitous chlorophyll a (Chl a) pigment absorbs both blue and red light. Yet, in contrast to green algae and higher plants, most cyanobacteria have much lower photosynthetic rates in blue than in red light. A plausible but not yet well-supported hypothesis is that blue light results in limited energy transfer to photosystem II (PSII), because...
Article
Full-text available
Several studies have described that cyanobacteria use blue light less efficiently for photosynthesis than most eukaryotic phototrophs, but comprehensive studies of this phenomenon are lacking. Here, we study the effect of blue (450 nm), orange (625 nm), and red (660 nm) light on growth of the model cyanobacterium Synechocystis sp. PCC 6803, the gre...
Article
Harmful cyanobacteria producing toxic microcystins are a major concern in water quality management. In recent years, hydrogen peroxide (H2O2) has been successfully applied to suppress cyanobacterial blooms in lakes. Physiological studies, however, indicate that microcystin protects cyanobacteria against oxidative stress, suggesting that H2O2 additi...
Chapter
In this chapter a DNA Chip assay is proposed that builds upon earlier experience from the fabrication of a DNA Chip targeted towards 16S rRNA genes of cyanobacteria. The principle is PCR amplification of the target mcyE/ndaF gene, followed by a specific ligation reaction that depends on two probes, one that is discriminative for the genotype and th...
Article
Full-text available
To help ban the use of general toxic algicides, research efforts are now directed towards the discovery of compounds that are specifically acting as cyanocides. Here, we review the past and look forward into the future, where the less desirable general algicides like copper sulphate, diuron or endothall may become replaced by compounds that show be...
Article
Full-text available
Rising atmospheric CO2 concentrations are likely to affect many ecosystems worldwide. However, to what extent elevated CO2 will induce evolutionary changes in photosynthetic organisms is still a major open question. Here, we show rapid microevolutionary adaptation of a harmful cyanobacterium to changes in inorganic carbon (Ci) availability. We stud...
Article
Full-text available
Dense phytoplankton blooms in eutrophic waters often experience large daily fluctuations in environmental conditions. We investigated how this diel variation affects in situ gene expression of the CO2-concentrating mechanism (CCM) and other selected genes of the harmful cyanobacterium Microcystis aeruginosa. Photosynthetic activity of the cyanobact...
Article
In the family of chlorophyll binding proteins, single helix small CAB-like proteins (SCPs) are found in all organisms performing oxygenic photosynthesis. Here, we investigated the function of these stress-inducible proteins in the cyanobacterium Synechocystis sp. PCC 6803. We compared physiological, proteome and transcriptome traits of a Photosyste...
Article
Full-text available
The societal importance of renewable carbon-based commodities and energy carriers has elicited a particular interest for high performance phototrophic microorganisms. Selection of optimal strains is often based on direct comparison under laboratory conditions of maximal growth rate or additional valued features such as lipid content. Instead of rep...
Article
Full-text available
Cyanobacteria are generally assumed to be effective competitors at low CO2 levels because of their efficient CO2-concentrating mechanism (CCM), yet how bloom-forming cyanobacteria will respond to rising CO2 concentrations is less clear. Here, we investigate changes in CCM gene expression at ambient CO2 (400 ppm) and elevated CO2 (1100 ppm) in six s...
Article
Microcystis aeruginosa is a ubiquitous harmful cyanobacterium that causes problems in eutrophic lakes. Potassium ion (K(+)) addition is one of the suggested methods to combat harmful cyanobacterial blooms. To investigate the effectiveness of this method, we compared the potassium ion sensitivity of four Microcystis strains. Microcystis strains PCC...
Article
Full-text available
Experiments with different phytoplankton densities in lake samples showed that a high biomass increases the rate of hydrogen peroxide (HP) degradation and decreases the effectiveness of HP in the selective suppression of dominant cyanobacteria. However, selective application of HP requires usage of low doses only, accordingly this defines the limit...
Article
Full-text available
Rising CO2 concentrations may have large effects on aquatic microorganisms. In this study, we investigated how elevated pCO2 affects the harmful freshwater cyanobacterium Microcystis aeruginosa. This species is capable of producing dense blooms and hepatotoxins called microcystins. Strain PCC 7806 was cultured in chemostats that were shifted from l...
Article
Full-text available
Mixotrophy is increasingly recognized as an important and widespread nutritional strategy in various taxonomic groups ranging from protists to higher plants. We hypothesize that the availability of alternative carbon and energy sources during mixotrophy allows a switch to photoheterotrophic growth, where the photosynthetic apparatus mainly provides...
Article
Full-text available
Recent studies have provided evidence for both intracellular and extracellular roles of the potent hepatotoxin microcystin (MC) in the bloom-forming cyanobacterium Microcystis. Here, we surveyed transcriptomes of the wild-type strain M. aeruginosa PCC 7806 and the microcystin-deficient ΔmcyB mutant under low light conditions with and without the ad...
Article
Full-text available
A method is presented for rapid extraction of the total plastoquinone pool from Synechocystis PCC6803 cells that preserves the in vivo plastoquinol (PQH2) to plastoquinone (PQ) ratio. Cells were rapidly transferred into ice-cold organic solvent for instantaneous extraction of the cellular PQ plus PQH2 content. After HPLC fractionation of the organi...
Chapter
Full-text available
The detailed knowledge already available about cellulose synthases and their regulation, plus emerging insights into the process of cellulose secretion in cyanobacteria make cellulose an attractive polymer for the application of the photanol concept in an economically viable production process. By varying the total complement of antenna pigments, t...
Article
Incompatibility of nitrogen fixation and oxygen production compel unicellular diazotrophic cyanobacteria to perform photosynthesis during daytime and restrict nitrogen fixation to nighttime. The marine diazotroph Cyanothece BG 043511 was grown in continuous culture under three light:dark regimes (16L:8D, 12L:12D and 8L:16D hours); we monitored nitr...
Article
Full-text available
Rising CO2 levels may act as an important selective factor on the CO2-concentrating mechanism (CCM) of cyanobacteria. We investigated genetic diversity in the CCM of Microcystis aeruginosa, a species producing harmful cyanobacterial blooms in many lakes worldwide. All 20 investigated Microcystis strains contained complete genes for two CO2 uptake s...
Article
Full-text available
Adjustment of gene expression during acclimation to stress conditions, such as bright light, in the cyanobacterium Synechocystis sp. PCC 6803 depends on four group 2 σ factors (SigB, SigC, SigD, SigE). A ΔsigCDE strain containing the stress-responsive SigB as the only functional group 2 σ factor appears twice as resistant to photoinhibition of phot...
Conference Paper
Full-text available
Previously, treatment with hydrogen peroxide (H2O2) successfully suppressed cyanobacterial blooms in lakes. We adapted this treatment to battle a toxic Alexandrium ostenfeldii bloom which threatened shell fisheries. Using a three-step approach including laboratory experiments, a field pilot study and finally entire Kreek treatment, cell numbers wer...
Article
Full-text available
Using metabolic and transcriptomic phenotyping, we studied acclimation of cyanobacteria to low inorganic carbon (LC) conditions and the requirements for coordinated alteration of metabolism and gene expression. To analyse possible metabolic signals for LC sensing and compensating reactions, the carboxysome-less mutant ΔccmM and the photorespiratory...
Article
Full-text available
Sequential adaptation to nitrogen deprivation and ultimately to full starvation requires coordinated adjustment of cellular functions. We investigated changes in gene expression and cell physiology of the cyanobacterium Synechocystis PCC 6803 during 96 h of nitrogen starvation. During the first 6 h, the transcriptome showed activation of nitrogen u...
Article
Although harmful cyanobacteria form a major threat to water quality, few methods exist for the rapid suppression of cyanobacterial blooms. Since laboratory studies indicated that cyanobacteria are more sensitive to hydrogen peroxide (H(2)O(2)) than eukaryotic phytoplankton, we tested the application of H(2)O(2) in natural waters. First, we exposed...
Article
Full-text available
Physiological adaptation and genome-wide expression profiles of the cyanobacterium Synechocystis sp. strain PCC 6803 in response to gradual transitions between nitrogen-limited and light-limited growth conditions were measured in continuous cultures. Transitions induced changes in pigment composition, light absorption coefficient, photosynthetic el...
Article
Photoacoustic spectroscopy was used to monitor photosynthetic energy storage in heterocysts isolated from the cyanobacterium Anabaena 7120. Three main energy-conversion pathways have been identified in these specialized cells: cyclic electron transport in photosystem I, nitrogen fixation, and respiration. The aim of this study was to define the con...
Article
Planktothrix agardhii is a widespread harmful cyanobacterium of eutrophic waters, and can produce the hepatotoxins [Asp(3)]microcystin-LR and [Asp(3)]microcystin-RR. These two microcystin variants differ in their first variable amino acid position, which is occupied by either leucine (L) or arginine (R). Although microcystins are extensively invest...
Article
Full-text available
Bioassays with the toxic cyanobacterium Microcystis aeruginosa PCC 7806, its non-toxic mutant Delta mcyB, and Daphnia magna as grazer were used to evaluate biotic factors in induced defence, in particular cyanobacterial and grazer-released info-chemicals. Three main questions were addressed in this study: Does Daphnia grazing lead to a loss of cyan...
Article
Full-text available
Blauwalgen vormen een groot probleem voor waterbeheerders en recreanten. De Universiteit van Amsterdam en ARCADIS ontwikkelden een nieuwe methode om blauwalgen te bestrijden door middel van waterstofperoxide. De toepassing en resultaten van de veldexperimenten worden hier gepresenteerd. Met het waterkwaliteitsmodel PCLake is de effectiviteit van de...
Article
Full-text available
In cyanobacteria, photorespiratory 2-phosphoglycolate (2PG) metabolism is mediated by three different routes, including one route involving the glycine decarboxylase complex (Gcv). It has been suggested that, in addition to conversion of 2PG into non-toxic intermediates, this pathway is important for acclimation to high-light. The photoreduction of...
Article
Transcript profiling of nitrate-grown Synechocystis sp. PCC 6803 PsbO-free mutant cells in comparison to wild-type (WT) detected substantial deviations. Because we had previously observed phenotypical differences between Synechocystis sp. PCC 6803 WT and its corresponding PsbO-free mutant when cultivated with l-arginine as sole N source and a light...
Article
The effect of simultaneous N2 fixation and light limitation on the growth of two strains of Anabaena sp. Bory de St. Vincent and Aphanizomenon flos-aquae (L.) Ralfs was investigated using continuous cultures. Under severely light-limited conditions, Aphanizomenon showed a broader absorption spectrum (due to the presence of phycoerythrin), a higher...
Article
Cyanobacteria are capable of producing multiple microcystin variants simultaneously. The mechanisms that determine the composition of microcystin variants in cyanobacteria are still debated. [Asp(3)]microcystin-RR contains arginine at the position where the more toxic [Asp(3)]microcystin-LR incorporates leucine. We cultured the filamentous cyanobac...
Data
Full-text available
Distribution of genome lengths for several cyanobacterial genomes.
Data
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Schematic representation of the phosphate transport gene cluster.
Data
Full-text available
Distribution of the intergenic distances in cyanobacterial and other bacterial genomes.
Data
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Putative methylases and methyltransferases in the genome of Mic-PCC7806.
Data
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Occurrence of the putative dnd gene products in cyanobacteria.
Data
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Fermentation pathway adapted from Moezelaar and Stal [34].
Data
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Genes of the circadian clock system.
Data
Representation of the location of the genes of Mic-PCC7806-contig328 on the genome of Mic-NIES843.
Data
Full-text available
Similarity of orthologous genes between Mic-PCC7806, Cwa-WH8501 and Syn-PCC6803.
Data
Identity of the 1% 6-mers that were least common in the genome of Mic-PCC7806.
Data
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Putative restriction endonucleases in the genome of Mic-PCC7806.
Data
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Alignment of the amino acid sequences of the putative caspases from Mic-PCC7806, Mic-NIES843 and a metacaspase from Saccharomyces cerevisiae.
Article
The PsaE protein is located at the reducing side of photosystem I (PSI) and is involved in docking the soluble electron acceptors, particularly ferredoxin. However, deletion of the psaE gene in the cyanobacterium Synechocystis sp. strain PCC 6803 inhibited neither photoautotrophic growth, nor in vivo linear and cyclic electron flows. Using photoaco...
Article
Full-text available
The colonial cyanobacterium Microcystis proliferates in a wide range of freshwater ecosystems and is exposed to changing environmental factors during its life cycle. Microcystis blooms are often toxic, potentially fatal to animals and humans, and may cause environmental problems. There has been little investigation of the genomics of these cyanobac...
Article
The role of the primary-like sigma factor SigC was studied in Synechocystis. Under high temperature stress (48 degrees C) the DeltasigC inactivation strain showed a lower survival rate than the control strain. The DeltasigC strain grew poorly at 43 degrees C in liquid cultures under normal air. However, change to 3% CO(2) enhanced growth of Deltasi...
Chapter
Despite the presence of a carbon concentrating mechanism, cyanobacteria synthesize 2- phosphoglycolate (2PG) by the oxygenase reaction of Rubisco. Using DNA-microarray analyses, we examined the regulation of genes involved in 2PG metabolism in the cyanobacterium Synechocystis PCC 6803. While genes for the plant-like C2 cycle were barely influenced...
Article
Cyanobacteria respond to iron deficiency during growth by expressing the isiA gene, which produces a chlorophyll-carotenoid protein complex known as IsiA or CP43'. Long-term iron deficiency results in the formation of large IsiA aggregates, some of which associate with photosystem I (PSI) while others are not connected to a photosystem. The fluores...
Technical Report
We compare the carotenoid composition and the quenching properties of IsiA aggregates obtained from a Synechocystis PCC 6803 psaFJ-null mutant and from a crtO-null mutant that is unable to synthesize echinenone. Pigment analysis indicates that isolated IsiA aggregates from the psaFJ-null mutant bind, per IsiA monomer, about 16 chlorophyll a, 2.2 β-...
Article
The sensitivity of phytoplankton species for hydrogen peroxide (H2O2) was analyzed by pulse amplitude modulated (PAM) fluorometry. The inhibition of photosynthesis was more severe in five tested cyanobacterial species than in three green algal species and one diatom species. Hence the inhibitory effect of H2O2 is especially pronounced for cyanobact...
Article
Full-text available
The photosynthetic pigments of phototrophic microorganisms cover different regions of the solar light spectrum. Utilization of the light spectrum can be interpreted in terms of classical niche theory, as the light spectrum offers opportunities for niche differentiation and allows coexistence of species absorbing different colors of light. However,...
Article
Full-text available
Concerted changes in the transcriptional pattern and physiological traits that result from long-term (here defined as up to 24 h) limitation of inorganic carbon (C(i)) have been investigated for the cyanobacterium Synechocystis sp. strain PCC 6803. Results from reverse transcription-polymerase chain reaction and genome-wide DNA microarray analyses...