Hanno Teeling

Hanno Teeling
Max Planck Institute for Marine Microbiology | MPI · Group of Microbial Genomics

Dr. rer. nat. (Dipl.-Chem. Dipl.-Biol.)

About

130
Publications
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Publications

Publications (130)
Article
Full-text available
Microbial glycan degradation is essential to global carbon cycling. The marine bacterium Salegentibacter sp. Hel_I_6 (Bacteroidota) isolated from seawater off Helgoland island (North Sea) contains an α-mannan inducible gene cluster with a GH76 family endo-α-1,6-mannanase (ShGH76). This cluster is related to genetic loci employed by human gut bacter...
Article
Full-text available
Background The planktonic bacterial community associated with spring phytoplankton blooms in the North Sea is responsible for a large amount of carbon turnover in an environment characterised by high primary productivity. Individual clades belonging to the Gammaproteobacteria have shown similar population dynamics to Bacteroidetes species , and are...
Article
Full-text available
Algal blooms produce large quantities of organic matter that is subsequently remineralised by bacterial heterotrophs. Polysaccharide is a primary component of algal biomass. It has been hypothesised that individual bacterial heterotrophic niches during algal blooms are in part determined by the available polysaccharide substrates present. Measureme...
Article
Full-text available
The formation of sinking particles in the ocean, which promote carbon sequestration into deeper water and sediments, involves algal polysaccharides acting as an adhesive, binding together molecules, cells and minerals. These as yet unidentified adhesive polysaccharides must resist degradation by bacterial enzymes or else they dissolve and particles...
Article
Full-text available
Massive releases of organic substrates during marine algal blooms trigger growth of many clades of heterotrophic bacteria. Algal polysaccharides represent the most diverse and structurally complex class of these substrates, yet their role in shaping the microbial community composition is poorly understood. We investigated, whether polysaccharide ut...
Article
Full-text available
Since the discovery of archaeoplankton in 1992, the euryarchaeotal Marine Group II (MGII) remains uncultured and less understood than other planktonic archaea. We characterized the seasonal dynamics of MGII populations in the southern North Sea on a genomic and microscopic level over the course of four years. We recovered 34 metagenome-assembled ge...
Article
Full-text available
We investigated Bacteroidetes during spring algae blooms in the southern North Sea in 2010–2012 using a time series of 38 deeply sequenced metagenomes. Initial partitioning yielded 6455 bins, from which we extracted 3101 metagenome-assembled genomes (MAGs) including 1286 Bacteroidetes MAGs covering ~120 mostly uncultivated species. We identified 13...
Article
Full-text available
Epibacterium mobile BBCC367 is a marine bacterium that is common in coastal areas. It belongs to the Roseobacter clade, a widespread group in pelagic marine ecosystems. Species of the Roseobacter clade are regularly used as models to understand the evolution and physiological adaptability of generalist bacteria. E. mobile BBCC367 comprises two chro...
Article
Full-text available
Marine microscopic algae carry out about half of the global carbon dioxide fixation into organic matter. They provide organic substrates for marine microbes such as members of the Bacteroidetes that degrade algal polysaccharides using carbohydrate‐active enzymes (CAZymes). In Bacteroidetes genomes CAZyme encoding genes are mostly grouped in distinc...
Article
Full-text available
Marine algae convert a substantial fraction of fixed carbon dioxide into various polysaccharides. Flavobacteriia that are specialized on algal polysaccharide degradation feature genomic clusters termed polysaccharide utilization loci (PULs). As knowledge on extant PUL diversity is sparse, we sequenced the genomes of 53 North Sea Flavobacteriia and...
Article
Microbial degradation of algal biomass following spring phytoplankton blooms has been characterised as a concerted effort among multiple clades of heterotrophic bacteria. Despite their significance to overall carbon turnover, many of these clades have resisted cultivation. One clade known from 16S rRNA gene sequencing surveys at Helgoland in the No...
Article
Full-text available
Polysaccharide degradation by heterotrophic microbes is a key process within Earth’s carbon cycle. Here, we use environmental proteomics and metagenomics in combination with cultivation experiments and biochemical characterizations to investigate the molecular details of in situ polysaccharide degradation mechanisms during microalgal blooms. For th...
Article
Full-text available
Temperate coastal marine environments are replete with complex biotic and abiotic interactions that are amplified during spring and summer phytoplankton blooms. During these events, heterotrophic bacterioplankton respond to successional releases of dissolved organic matter as algal cells are lysed. Annual seasonal shifts in the community compositio...
Article
Gammaproteobacterial Reinekea spp. were detected during North Sea spring algae blooms in the years 2009-2012, with relative abundances of up to 16% in the bacterioplankton. Here we explore the ecophysiology of 'R. forsetii' strain Hel1_31_D35 that was isolated during the 2010 spring bloom using (i) its manually annotated, high-quality closed genome...
Article
Full-text available
The marine flavobacterium Zobellia galactanivorans DsijT was isolated from a red alga and by now constitutes a model for studying algal polysaccharide bioconversions. We present an in-depth analysis of its complete genome and link it to physiological traits. Z. galactanivorans exhibited the highest gene numbers for glycoside hydrolases, polysacchar...
Article
Full-text available
Marine Bacteroidetes have pronounced capabilities of degrading high molecular weight organic matter such as proteins and polysaccharides. Previously we reported on 76 Bacteroidetes-affiliated fosmids from the North Atlantic Ocean's boreal polar and oligotrophic subtropical provinces. Here we report on the analysis of further 174 fosmids from the sa...
Data
Spearman rank correlations of abundant Bacteroidetes clades to phytoplankton groups. Correlation values were considered only when p-value <0.05. Spearman rank correlations measure the strength of association between two ranked variables. A precondition (assumption) for this nonparametric statistical analysis is that the two variables share a monoto...
Data
E-value thresholds used for automated CAZyme family detection. Searches were performed against the CAZy database, the dbCAN database and the Pfam database using E-value thresholds that were adjusted for each family by extensive manual annotations. CAZymes were only annotated when at least two of the three database searches yielded positive results...
Data
Physicochemical parameters. DOI: http://dx.doi.org/10.7554/eLife.11888.011
Data
Cell counts of dominating phytoplankton. DOI: http://dx.doi.org/10.7554/eLife.11888.012
Data
Total cell and CARD-FISH cell counts of bacterioplankton. DOI: http://dx.doi.org/10.7554/eLife.11888.013
Data
Linear regression analyses were computed in order to test, whether the abundances of major clades of Flavobacteriia were influenced by abiotic factors or by abundant algae groups. For all tested clades of Flavobacteriia, multiple abiotic factors and multiple algae groups were obtained as explanatory variables. The strongest abiotic predictors were...
Data
Specific oligonucleotide probes used for quantification of free-living (0.2 - 3 µm) bacterioplankton populations by fluorescence in situ hybridization (FISH). DOI: http://dx.doi.org/10.7554/eLife.11888.014
Data
Abundance matrix of the 16S rRNA gene tag sequence analysis. DOI: http://dx.doi.org/10.7554/eLife.11888.015
Data
Statistics of the 16 metagenomes from free-living (0.2 - 3.0 µm) North Sea bacterioplankton that were used in this study. Two additional metagenomes are listed in italics for completeness, one from a test run sampled on August 21th, 2008, and a metagenome of particle-attached (3 - 10 µm) bacterioplankton sampled on April 14th, 2009. DOI: http://dx....
Data
Sizes in basepairs of the metagenome taxonomic bins that were used for CAZyme frequency analyses on order to genus level. Taxonomic bins with too small sizes for a sound analysis were excluded (red text). DOI: http://dx.doi.org/10.7554/eLife.11888.020
Poster
Primary production by blooming algae can triggers consecutive blooms of distinct clades of planktonic bacteria. By combined metaproteome and metagenome analyses we found indications for resource partitioning between abundant clades of Flavobacteriia and Gammaproteobacteria during a North Sea spring algae bloom in 2009 [1]. Our data suggest that mem...
Article
Full-text available
The dynamics of coastal marine microbial communities are driven by seasonally changing abiotic and biotic factors as well as by rapidly occurring short-term changes such as river fresh-water influxes or phytoplankton blooms. We examined the variability of the free-living bacterioplankton at Helgoland Roads (German Bight, North Sea) over a period of...
Conference Paper
Introduction: In 2009 we investigated the response of bacterioplankton to the release of algal-derived organic compounds during a spring phytoplankton bloom in the North Sea. We observed a swift succession of distinct bacterioplankton clades with different ecological niches, likely due to changes in substrate availability over time [1]. Within the...
Article
Full-text available
Members of the flavobacterial genus Polaribacter thrive in response to North Sea spring phytoplankton blooms. We analyzed two respective Polaribacter species by whole genome sequencing, comparative genomics, substrate tests and proteomics. Both can degrade algal polysaccharides but occupy distinct niches. The liquid culture isolate Polaribacter sp....