Hannah Miriam Jaag

Hannah Miriam Jaag
University of Kentucky | UKY · Department of Plant Pathology

Dr. rer. nat.

About

16
Publications
975
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
328
Citations
Additional affiliations
September 2005 - December 2010
University of Kentucky

Publications

Publications (16)
Article
Viruses change rapidly due to genetic mutations, and viral RNA recombination in RNA viruses can lead to the emergence of drug-resistant or highly virulent strains. Here, we report that host Pmr1p, an ion pump that controls Ca2+/Mn2+ influx into the Golgi from the cytosol, affects the frequency of viral RNA recombination and the efficiency of replic...
Article
Full-text available
Viruses are masters of evolution due to high frequency mutations and genetic recombination. In spite of the significance of viral RNA recombination that promotes the emergence of drug-resistant virus strains, the role of host and environmental factors in RNA recombination is poorly understood. Here we report that the host Met22p/Hal2p bisphosphate-...
Article
Full-text available
Potato leafroll polerovirus (PLRV) genomic RNA acts as a polycistronic mRNA for the production of proteins P0, P1, and P2 translated from the 5'-proximal half of the genome. Within the P1 coding region we identified a 5-kDa replication-associated protein 1 (Rap1) essential for viral multiplication. An internal ribosome entry site (IRES) with unusua...
Chapter
Full-text available
Preprint
Full-text available
Cyclamen purpurascens (Alpine, European or purple cyclamen) is native to central Europe. Since decades it is discussed wether the occurrences of C. purpurascens north of the alps is native or if it was introduced. Here the nuclear ribosomal DNAs (rDNA) are sequenced in oder to obtain a phylogenetic geographic pattern. Phylogenetic analyses of ITS a...
Data
Yeast strains, expression plasmids and primers. (0.12 MB PDF)
Data
Full-text available
LiCl enhances the formation and accumulation of recRNAs and degRNAs in yeast. (A) Northern blot analysis of total RNA samples from yeast replicating TBSV DI-72 repRNA. BY4741 yeast cultures were treated with the shown concentration of LiCl. Samples were taken 24 hours after launching TBSV repRNA replication. Note that the repRNA was expressed from...
Data
Increased level of accumulation of the nondegraded ITS1 region of pre-ribosomal RNA after LiCl treatment. The activity of cellular 5′-3′ exoribonucleases, such as Xrn1p (cytosolic) and Rat1p (nucleus), was inhibited by the shown amount of LiCl as described. As expected, LiCl treatment increased the accumulation of pre-ribosomal RNA carrying the ITS...
Article
Full-text available
RNA degradation, together with RNA synthesis, controls the steady-state level of viral RNAs in infected cells. The endoribonucleolytic cleavage of viral RNA is important not only for viral RNA degradation but for RNA recombination as well, due to the participation of some RNA degradation products in the RNA recombination process. To identify host e...
Article
Full-text available
Full-length infectious cDNA clones of Canadian (PLRVflc) and German (PLRVflg) Potato leafroll virus isolates were constructed and their biological activities examined in planta. For both constructs, agroinfection by PLRV cDNA resulted in virus multiplication in the primary leaf followed by systemic movement and the production of icosohedral PLRV pa...
Article
The cytosolic 5'-to-3' exoribonuclease Xrn1p plays a major role in recombination and degradation of Tomato bushy stunt tombusvirus (TBSV) replicon (rep)RNA in yeast, a model host (Serviene, E., Shapka, N., Cheng, C.P., Panavas, T., Phuangrat, B., Baker, J., and Nagy, P.D., 2005. Genome-wide screen identifies host genes affecting viral RNA recombina...
Article
Previous genome-wide screens identified over 100 host genes whose deletion/down-regulation affected tombusvirus replication and 32 host genes that affected tombusvirus RNA recombination in yeast, a model host for replication of Tomato bushy stunt virus (TBSV). Down-regulation of several of the identified host genes affected the accumulation levels...
Article
Rapid RNA virus evolution is a major problem due to the devastating diseases caused by human, animal and plant-pathogenic RNA viruses. A previous genome-wide screen for host factors affecting recombination in Tomato bushy stunt tombusvirus (TBSV), a small monopartite plant virus, identified Xrn1p 5'-3' exoribonuclease of yeast, a model host, whose...
Article
Full-text available
Forisomes are elongate protein bodies of up to 30 µm length from cells of the sieve tube network of higher plants. In this natural microfluidics system, they act as reversible stopcocks by undergoing rapid conformational changes which involve more than three-fold increases in volume. The conformational switch is controlled by Ca 2+ with a threshhol...

Network

Cited By