Hanna Vuorenpää

Hanna Vuorenpää
Tampere University | UTA · Faculty of Medicine and Health Technology

Doctor of Philosophy

About

14
Publications
1,434
Reads
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134
Citations
Additional affiliations
August 2008 - present
Tampere University
Position
  • PhD Student
August 2008 - February 2016
Tampere University
Position
  • PhD

Publications

Publications (14)
Article
Full-text available
The vasculature is an essential, physiological element in virtually all human tissues. Formation of perfusable vasculature is therefore crucial for reliable tissue modeling. Three-dimensional vascular networks can be formed through the co-culture of endothelial cells (ECs) with stromal cells embedded in hydrogel. Mesenchymal stem/stromal cells (MSC...
Article
Full-text available
Objective: Imaging of tissue engineered three-dimensional (3D) specimens is challenging due to their thickness. We propose a novel multimodal imaging technique to obtain multi-physical 3D images and the electrical conductivity spectrum of tissue engineered specimensin vitro. Approach: We combine simultaneous recording of rotational multifrequenc...
Article
Full-text available
In vitro cell culture models representing the physiological and pathological features of the outer retina are urgently needed. Artificial tissue replacements for patients suffering from degenerative retinal diseases are similarly in great demand. Here, we developed a co-culture system based solely on the use of human induced pluripotent stem cell (...
Article
Full-text available
In order to translate preclinical data into the clinical studies, relevant in vitro models with structure and key functional properties similar to native human tissue should be used. In vitro cardiac models with vascular structures mimic the highly vascularized myocardium and provide interactions between endothelial cells, stromal cells and cardiom...
Article
The formation of blood vessels is a vital process in embryonic development and in normal physiology. Current vascular modelling is mainly based on animal biology leading to species-to-species variation when extrapolating the results to humans. Although there are a few human cell based vascular models available these assays are insufficiently charac...
Article
The interaction between different cardiac cells has shown to be important for critical biological properties including cell survival, proliferation, differentiation and function. The improvement of culture conditions with different cell types and to study their effects on cardiomyocyte viability and functionality is essential. For practical applica...
Article
Full-text available
The current limitation in designing three-dimensional tissue models is the lack of adequate vascularization with mature and stable vessels. Adipose tissue is known to secrete several angiogenic factors, and human adipose stromal cells (hASC) are known to promote vessel growth, maturation and stabilization. In this study, hASC were induced to angiog...
Article
Full-text available
The developed standardized human cell based in vitro angiogenesis assay was intra-laboratory pre-validated to verify that the method is reliable and relevant for routine testing of modulators of angiogenesis, e.g., pharmaceuticals and industrial chemicals. This assay is based on the earlier published method but it was improved and shown to be more...

Questions

Question (1)
Question
In an on-going project, I am trying to replace traditional animal-derived antibodies with animal-free substituents in immunocytochemical stainings of 2D and 3D cell cultures. Do you have experience on this and/or on animal-free secondary antibodies or fluorophore conjugates? Several questions are raised when aiming for replacement, so any help is deeply appreciated!

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Projects

Project (1)
Project
To establish Adipose Tissue-on-a-Chip