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September 2001 - present
Publications
Publications (52)
Kompozyty polimerowe ze względu na dobre właściwości przy stosunkowo niskiej cenie stanowią od wielu lat jedną z głównych grup materiałowych. W coraz większym stopniu zastępują one obecnie ceramikę, metale i czyste polimery. Co więcej, rozwój niektórych gałęzi przemysłu, np. motoryzacji lub elektroniki, nie jest aktualnie możliwy bez ich udziału. K...
Complexes of chlorogenic acid (5-CQA) with copper(II) and iron(III) were synthesized in a solid state and examined by means of FT-IR, thermogravimetric, and elemental analyses. The molar stoichiometric ratios of metal:ligand for the solid forms of the complexes were established as Cu(II):L = 1:2 and Fe(III):L = 2:3 (L: 5-CQA), with the possible coo...
Polyethylene is a model polyolefin, and a widely used material for the manufacture of many products, including cable sheaths. Understanding degradation mechanisms at the atomic scale leading to oxidation during aging is crucial for many long-term applications. The concentrations of radicals derived from oxidation and chain scission during radio-oxi...
Collagen is a biocompatible and bioresorbable material, an excellent active component for the formulation of medical dressings and scaffolds. Its current popularity and the developing methods of producing such medical materials imply the need for methods of controlling and processing collagen used in medicine. One of the basic methods used to steri...
The management of hard-to-heal wounds is a significant clinical challenge. Acellular dermal matrices (ADMs) have been successfully introduced to enhance the healing process. Here, we aimed to develop protocol for the preparation of novel ADMs from abdominoplasty skin. We used three different decellularization protocols for skin processing, namely,...
Wyniki badań w dziedzinie chemii radiacyjnej polimerów wykorzystuje się do oceny wpływu promieniowania jonizującego na właściwości wielu rodzajów obiektów o znaczeniu historycznym. Pod kątem radiacyjnej konserwacji opisano między innymi: farby akrylowe, pigmenty, kleje, naturalne i sztuczne włókna. Zupełnie nową dziedziną są artefakty wykonane z tw...
![Figure 1. Biological properties of quercetin [5-9].](publication/355122147/figure/fig1/AS:1076785722724353@1633737130994/Biological-properties-of-quercetin-5-9_Q320.jpg)
In this study a cobalt(II) complex of quercetin was synthetized in the solid state with the general formula Co(C15H9O7)2∙2H2O. The FT-IR, elemental analysis, and UV/Vis methods were used to study the composition of the complex in a solid state and in a water solution. The anti-/pro-oxidant activity of quercetin and the Co(II) complex was studied by...
![Figure 3. Classification of nanozymes [26,65,74]. (*) Mark represents...](publication/354911448/figure/fig1/AS:1073213828263936@1632885524392/Classification-of-nanozymes-26-65-74-Mark-represents-the-nanozymes-commonly-used_Q320.jpg)
Nanomaterials with enzyme-like activity (nanozymes) have found applications in various fields of medicine, industry, and environmental protection. This review discusses the use of nanozymes in the regulation of cellular homeostasis. We also review the latest biomedical applications of nanozymes related to their use in cellular redox status modifica...
Seven derivatives of plant-derived hydroxybenzoic acid (HBA)—including 2,3-dihydroxybenzoic (2,3-DHB, pyrocatechuic), 2,4-dihydroxybenzoic (2,4-DHB, β-resorcylic), 2,5-dihydroxybenzoic (2,5-DHB, gentisic), 2,6-dihydroxybenzoic (2,6-DHB, γ-resorcylic acid), 3,4-dihydroxybenzoic (3,4-DHB, protocatechuic), 3,5-dihydroxybenzoic (3,5-DHB, α-resorcylic),...
Gamma rays and electrons with kinetic energy up to 10 MeV are routinely used to sterilize biomaterials. To date, the effects of irradiation upon human acellular dermal matrices (hADMs) remain to be fully elucidated. The optimal irradiation dosage remains a critical parameter affecting the final product structure and, by extension, its therapeutic p...
The Mg(II) and heterometallic Mn(II)/Na(I) complexes of isoferulic acid (3-hydroxy-4-methoxycinnamic acid, IFA) were synthesized and characterized by infrared spectroscopy FT-IR, FT-Raman, electronic absorption spectroscopy UV/VIS, and single-crystal X-ray diffraction. The reaction of MgCl2 with isoferulic acid in the aqueous solutions of NaOH resu...
Gold nanoparticles (AuNPs) are foreseen as a promising tool in nanomedicine, both as drug carriers and radiosensitizers. They have been also proposed as a potential anticancer drug due to the anti-angiogenic effect in tumor tissue. In this work we investigated the effect of citrate-coated AuNPs of nominal diameter 20 nm on the growth and metastatic...
Low-density lipoprotein (LDL) preparations of the chosen polyphenols (PPs) were prepared for the first time in the literature. The solubility of the PPs in the lipidic core of the LDL increased with the increase of their lipophilicity. The anti-/pro-oxidative properties and toxicity of LDL-entrapped PPs toward A 2780 human ovarian cancer cells were...
The research was conducted in the “logical series” of seven ligands: chromone, flavone, 3-hydroxyflavone, 3,7-dihydroxyflavone, galangin, kaempferol and quercetin. Each subsequent ligand differs from the previous one, among others by an additional hydroxyl group. The studied chromone derivatives are plant secondary metabolites which play an importa...
![Fig. 4. Chemical structure of curcuminoids. Based on [98].](profile/R-Swislocka/publication/334477362/figure/fig2/AS:796147413168131@1566827743670/Chemical-structure-of-curcuminoids-Based-on-98_Q320.jpg)
![Fig. 6. Kanonical Src signaling. Based on [231]. Src is activated by...](profile/R-Swislocka/publication/334477362/figure/fig4/AS:796147413180426@1566827743922/Kanonical-Src-signaling-Based-on-231-Src-is-activated-by-many-RTKs-and-other-membrane_Q320.jpg)
Curcumin is a natural substance known for ages, exhibiting a multidirectional effect in cancer prevention and adjuvant cancer therapies. The great advantage of using nutraceuticals of vegetable origin in comparison to popular cytostatic drugs is the minimized side effect and reduced toxicity. The targets in oncological therapy are, among others, ty...
[Fe(NO)2] - modified nanoparticles of low-density protein (DNICLDL) can serve as conveyors of iron in the form of stable complexes with ApoB100 protein. As reported recently, in human hepatoma cells DNICLDL significantly increased the total iron content, while showing low toxicity. In the present work, we focused on the effects of internalization o...
In view of the interrelations between NO, Fe, and LDL in the cardiovascular system it appears interesting to find out, if the lipoprotein particles undergo the process of iron-nitrosylation, commonly observed for other proteins and what is the biological fate of iron-nitrosylated LDL particles. Iron-nitrosylated LDL preparation containing Fe(NO)2 m...
No abstract is available for this article.
Pyrazinecarboxylic acid and its derivatives show biological properties (inter alia antimicrobial and antifungal). In the frame of this work, the salts of 2-pyrazinecarboxylic and 2,3-pyrazinedicarboxylic acids with alkali metal cations were synthesized as well as the spectroscopic (IR, Raman, NMR), theoretical [density functional theory (DFT)] and...
![Table 4 . Chemical shift values [ppm] in 1 H and 13 C NMR spectra of...](publication/304369920/figure/tbl1/AS:613939113107481@1523385899715/Chemical-shift-values-ppm-in-1-H-and-13-C-NMR-spectra-of-2-6-PDA-determined_Q320.jpg)
In the presented work the thermal, theoretical (DFT) and spectroscopic (IR, Raman, NMR) properties of alkali metal complexes with quinolinic acid (2,3-pyridinedicarboxylic acid) and dipicolinic acid (2,6-pyridinedicarboxylic acid) were studied. The IR and Raman spectra were registered and analyzed in the range of 400-4000 cm-1. 1H NMR and 13C NMR s...
The cytoprotective and anticancer action of dietary in-taken natural polyphenols have for long been attributed only to their direct radical scavenging activities. Currently it is well supported that those compounds display a broad spectrum of biological and pharmacological outcomes mediated by their complex metabolism, interaction with gut microbio...
Dinitrosyl iron (I) complexes (DNICs), intracellular NO donors, are important factors in nitric oxide-dependent regulation of cellular metabolism and signal transduction. It has been shown that NO diminishes toxicity of iron ions and vice versa. To gain insight into a possible role of DNIC in this phenomenon, we examined the effect of DNIC(GS)2 for...
This work is a continuation of research on a correlation between the molecular structure and electronic charge distribution of phenolic compounds and their biological activity. The influence of lithium, sodium, potassium, rubidium and cesium cations on the electronic system of trans o-coumaric (2-hydroxy-cinnamic) acid was studied. We investigated...
Apples are among the most popular fruits in the world. They are rich in phenolic compounds, pectin, sugar, macro- and microelements. Applying different extraction techniques it is possible to isolate a particular group of compounds or individual chemicals and then test their biological properties. Many reports point to the antioxidant, antimicrobia...
A comprehensive overview is presented of the biologically relevant coordination chemistry of nitrosyls and its biochemical consequences. Representative classes of metal nitrosyls are introduced along with the structural and bonding aspects that may have consequences for the biological functioning of these complexes. Next, the biological targets and...
A comprehensive overview is presented of the biologically relevant coordination chemistry of nitrosyls and its biochemical consequences. Representative classes of metal nitrosyls are introduced along with the structural and bonding aspects that may have consequences for the biological functioning of these complexes. Next, the biological targets and...
Structural and spectroscopic data when combined with theoretical calculations provide adequate descriptions of the electronic structures of the nitrosyl moiety in nitric oxide(II) complexes. This concise overview discusses the spectroscopic features of metal nitrosylates. The results of IR, Raman, UV–Vis, EPR, Mössbauer, magnetic circular dichroism...
Food irradiation as a method of preservation is nearly a century old. Although it has been approved by the quality control in many countries around the world, it still remains a matter of controversy. There is no question that the process of food irradiation should be monitored, and irradiated foods should be subject to very strict quality control....
Nitrosyl complexes of iron are formed in living species in the presence of nitric oxide. They are considered a form in which NO can be stored and stabilized within a living cell. Upon entering a topic in bioinorganic chemistry the researcher faces a wide spectrum of issues concerning synthetic methods, the structure and chemical properties of the c...
Both the physical and chemical test results indicate that the DNA under different conditions can act as a narrow band semiconductor. The charge transfer along the DNA double strand molecule depends both on the nucleotide sequence and the distance, and is possible only in the case of an ideal paring of both strands. The charge transfer within DNA ma...
Formation of dinitrosyl iron complexes (DNICs), which can be described by general formula Fe(NO)2(L)2, where L is carbonyl-, nitrosyl- or imino- complexing ligand, was observed in many kinds of living organisms, in a wide spectrum of physiological conditions associated with inflammation, ischemia/reperfusion and cancer. Accumulation of DNICs coinci...
A prerequisite of dinitrosyl iron complexes (DNIC) formation is the presence of nitric oxide (NO), iron (Fe) and thiol/imidazole groups. The aim of this study was to investigate the influence of Fe chelators on the formation of DNIC in erythroid K562 cells. The cells were treated with lipophilic salicylaldehyde isonicotinoyl hydrazone (SIH) (0.1 mM...
Abstract Dinitrosyl non-heme–iron complexes (DNIC) are found in many nitric oxide producing tissues. A prerequisite of DNIC formation is the presence of nitric oxide, iron and thiol/imidazole groups. The aim of this study was to investigate the role of the cellular labile iron pool in the formation of DNIC in erythroid K562 cells. The cells were tr...
Formation of dinitrosyl iron complexes (DNIC), with the general formula Fe(NO)2(L)2, was observed in many species of plants, animals and bacteria. They are important in human physiology and pathology, including inflammatory states, Parkinson disease, and malignancy. The report presents in detail the electron and steric structure of DNIC, electron p...
This paper reviews the results of the intense experimental and theoretical studies on the influence of selected metals on the electronic system of biologically important molecules such as benzoic, 2-hydroxybenzoic and 3-pyridine carboxylic acids as well as 5-carboxyuracil. The research involved following techniques: infrared (FT-IR), Raman (FT-Rama...
Halogenobenzoates and 5-halogenouracils are commonly applied in industrial and medical fields. From this point of view, it is very important to study the effect of halogens and metal on the above mentioned biologically important ligands. This paper reviews the results of many years’ studies on the influence of halogens (in the series: F → Cl → Br →...
![Fig. 1 Schematic molecular structure of cis-[Pt()Cl 2 (V)] and...](profile/Hanna-Lewandowska/publication/244503606/figure/fig3/AS:667808169992192@1536229283074/Schematic-molecular-structure-of-cis-PtCl-2-V-and-cisPdCl-2-V-complexes_Q320.jpg)
To develop new complexes of Pt(II) and Pd(II) that have ligands in a shape complementary to DNA, we designed and synthesized a methyl 3,4-diamino-2,3,4,6-tetradeoxy-α-L-lyxo-hexopyranoside (V). This synthetic ligand imitates the shape of daunosamine, a DNA minor groove binding sugar moiety present in the antineoplastic anthracycline antibiotics dox...
The paper reviews the recent reports on the role of the phosphorylated histone H2AX (gamma-H2AX). The modification of this historic is an important part of the cellular response to the induction of DNA double strand breaks (DSB) by ionising radiation and other DSB-generating factors. In irradiated cell the modification is carried out mainly by ATM...
Questions
Questions (8)
Dear Everyone. I am trying to do the high yield synthesis from ascorbate and copper salt, according to the similar procedures from the literature (0.18M copper ions) I now tried PVA up to 2%, Tween 20 up to 10%, both, none, dropwise addition of ascorbate, burst addition of ascorbate, nitrogen, air atmosphere, temperatures 50, 60, 90 centigrade. I followed a couple of similar syntheses from the literature, nothing works. All the time instead of suspension I get a salmon-colored precipitate that for sure is not a dozen nanometer copper. What do I do wrong?
Thanks a lot for all the hints
Please can anyone share the best procedure for the synthesis of copper nanoparticles using ascorbate in water? I need a maximum possible concentration and monodisperse size between 10 and 100 nm. What capping agent do you recommend?
I try to resolve collagen on SDS-PAGE. My goal is to resolve the collagen extracted from skin scaffolds. I use ThermoFisher Bolt mini gels. I can see the protein ladder migrating and also I can see BSA positive control. Yet I can see neither the collagen type I from Sigma nor the collagen from my samples. I dissolve collagen at 8 mg/mL in 0.5 M acetic acid. I tried to desalt it and add to the LDS buffer, add it without desalting, trypsinize it (then I can see the band from trypsin in my gel), reduce it, I did it with heating and without heating the LDS-treated sample. Certainly, the protein is there and I cannot see it precipitating in LDS. I tried to visualise it with Coomassie stain and with the silver stain, never have I seen any band from the collagen. It is not visible at the start of the gel either.
Can anyone help?
Thanks a lot in advance.
I'd be very gratefull for any info on availability of this line, I'd like to use it to monitor xenografts in mice. This model will be further used for radiodiagnostic affinity imaging. Thanks in advance
Please, give me your feedback on the AP/MALDI method. How is it comparable to classic MALDI? Would you invest in this module or rather buy the whole set of classic MALDI?
I'd appreciate a hint on a good resin-type preparation for elimination of transition metal traces. I need to purify my ligands, (GSH, small peptides, protein isolates, plasmids etc.) Is there anything commercially available?
I'd like to label my LDL in a manner that would not affect neither thyrosine or cysteine residues of my ApoB100. What does Dil do to the structure of a protein - I can't find it anywhere.
Projects
Projects (4)
Szkolenie obejmuje zastosowania promieniowania jonizującego do produkcji i modyfikacji wyrobów polimerowych, takich jak: uszczelki gumowe, opony, opakowania, hydrożele, osłony kabli do zastosowań specjalnych, osłonki i mufy termokurczliwe. Omawiane będą również kwestie: kompatybilizacji wyrobów polimerowych z powłokami lakierniczymi, sterylizacji i mikrobiologicznej dekontaminacji wyrobów medycznych, farmaceutyków, kosmetyków, produktów żywnościowych oraz obiektów o znaczeniu histotrycznym. Szkolenie jest przeznaczone dla wszystkich zainteresowanych przetwórstwem tworzyw sztucznych, sterylizacją i radiacyjnym recyclingiem, którzy chcą poszerzyć swoją ofertę o wyroby polimerowe charakteryzujące się podwyższonymi parametrami technicznymi, pamięcią kształtu, termokurczliwością, aktywnością powierzchniową, usieciowaniem itd. Przedstawiona zostanie również oferta w zakresie bezpiecznej i ekonomicznej metody radiacyjnej sterylizacji skierowana do przedsiębiorstw i ośrodków naukowych chcących nawiązać współpracę w ramach projektów przemysłowo-rozwojowych.
Więcej na: http://www.ichtj.waw.pl/drupal/?q=node/1239
Osoby do kontaktu:
Dr hab. Hanna Lewandowska, prof IChTJ h.lewandowska@ichtj.waw.pl
Dr Wojciech Głuszewski w.gluszewski@ichtj.waw.pl
Dear Colleagues,
This issue will focus on the newest findings on the role of metals in cell signaling, including all aspects of signal reception, transduction, and response. The tentative subjects include the following:
metal cofactor-driven signaling mechanisms,
oxidation state-related cellular aspects of metal-dependent signaling,
oxidative stress and its role in metal-related cellular signaling pathways,
the influence of metals on cell homeostasis,
mechanisms of regulation of the metal levels in cells,
the influence of heavy metals on cellular regulation pathways,
metal-related cancer-signaling pathways,
metal-binding sites in proteins,
metallic sites regulation by signaling molecules,
special roles of metals in the organs analyzed at a cellular level,
metallic and metal-sensing probes applied in monitoring of cellular processes.
Both review and original papers are welcome. Authors are encouraged to suggest any other aspects of the metal-related cell signaling, according to their knowledge and expertise.
Dr. Hanna Lewandowska
Guest Editor
TeaM Cables – European Tools and Methodologies for an efficient ageing management of nuclear power plant Cables – is a research project in the field of nuclear safety and long-term operation. TeaM Cables aims at providing NPP operators with a novel methodology for efficient and reliable NPP cable ageing management.
The project has received a funding of 4.2 million Euro from the Horizon 2020 Euratom Research and Training Programme 2014-2018 of the European Union under grant agreement No 755183.