Hang Li

• Ph.D.
• Assistant Professor at Beijing Institute of Technology

Evaporation poses a significant challenge in digital microfluidics (DMF) systems, especially when microdroplets are exposed to air. Evaporation can increase substance concentration, resulting in distorted detection outcomes and even causing cell apoptosis. In our research, we systematically evaluated the effects of various factors on droplet evapor...

The development of advanced intracellular delivery systems is essential for biopharmaceutical progress, particularly in drug delivery systems, genetic engineering, and cellular therapeutics. While nanovesicles show significant therapeutic potential, challenges remain due to cell membrane barriers and the limitations of endocytosis-mediated pathways...

Optoelectronic tweezers (OET) offer a versatile, programmable, and contactless method for manipulating microscale objects. While factors like AC voltage and light intensity have been extensively studied, the role of light pattern curvature in the performance of OET manipulation remains underexplored. This study investigates how the curvature of lig...

Optical tweezers, with their high precision, dynamic control, and non-invasiveness, are increasingly important in scientific research and applications at the micro and nano scales. However, manipulation by optical tweezers is challenged by adsorption forces, including van der Waals forces, capillary forces, and electrostatic forces, which are prese...

Sorting specific cells from heterogeneous samples is important for research and clinical applications. In this work, a novel label‐free cell sorting method is presented that integrates deep learning image recognition with microfluidic manipulation to differentiate cells based on morphology. Using an Active‐Matrix Digital Microfluidics (AM‐DMF) plat...

Optical manipulation technology encompasses a suite of micromanipulation techniques that employ light to control and actuate microscopic objects. As a valuable scientific tool, optical manipulation technology is employed by researchers to investigate fundamental biological processes, examine the mechanics of microstructures, and develop innovative...

Continuum robots with their inherent compliance provide the potential for crossing narrow unstructured workspace and safely grasping various objects. However, the display gripper increases the size of the robots, and therefore, it tends to get stuck in constrained environments. This paper proposes a versatile continuum grasping robot (CGR) with a c...

Profiling proteins plays an essential role in understanding the functions and dynamic networks in biological systems. Mass spectrometry-based proteomic analysis commonly requires multistep sample processing, which results in severe sample loss. Although the recently developed microproteomic strategies have substantially reduced sample loss via drop...

Exosomes have great potential as biomarker carriers for disease diagnosis and prognosis. In recent years, exosomal RNA (exoRNA) has become a promising candidate for the early diagnosis and prognosis of cancers, and its pathophysiological roles in various diseases have been revealed. For example, exosome-derived mRNAs, miRNAs, circRNAs, and lncRNAs...

Extracellular vesicles (EVs) are membranous vesicles released by cells that carry a number of biologically important components such as lipids, proteins, and mRNAs. EVs can mediate cancer cell migration, invasion, angiogenesis, and cell survival, greatly contributing to cell-to-cell communication in the tumor microenvironment. Additionally, EVs hav...

Extracellular vesicles (EVs) released from tumor cells and tissues mediate intercellular communication and regulate various important biological processes. The characterization of EVs can provide potential diagnostic biomarkers and therapeutic targets. However, current EV enrichment and sample preparation methods for omics analysis are time-consumi...

氧连接氮乙酰葡萄糖胺(O-GlcNAc)是一种重要的蛋白质翻译后修饰,它在维持机体正常的生命活动中发挥着重要作用。许多研究证实,O-GlcNAc糖基化修饰稳态的破坏与人类多种疾病的发生相关,大规模富集鉴定O-GlcNAc糖基化修饰蛋白有助于发现新的临床疾病诊断标志物。由于O-GlcNAc糖基化修饰丰度较低,形成的糖苷键不稳定,O-GlcNAc糖基化修饰蛋白/肽段的富集鉴定面临一定挑战。近年来,全乙酰化的非天然糖代谢标记技术被广泛应用于O-GlcNAc糖基化修饰蛋白/肽段的富集鉴定。然而,最新的研究发现,在细胞代谢标记过程中,全乙酰化的非天然单糖会同时标记半胱氨酸的巯基而引入半胱氨酸巯基-叠氮糖人为修饰物。该副反应在一定程度上干扰了O-GlcNAc糖基化修饰蛋白/肽段的富集鉴定。鉴于此,研究发...

蛋白质的N-糖基化是真核细胞中一种重要的翻译后修饰,N-糖基化修饰在调控细胞黏附、迁移、信号转导及细胞凋亡等方面扮演着关键角色。蛋白质糖基化修饰的异常变化与多种重要疾病的发生相关。尿液具有蛋白质组复杂程度低和非入侵性等特点,适合大量及连续多时间点采样研究。但由于个体差异和生理条件的影响,尿蛋白丰度的生理波动较大。目前缺乏对健康人群尿液N-糖蛋白的个体差异和生理波动的专门性研究,以及生理丰度范围的构建,难以将个体差异、正常生理波动和疾病导致的变化进行有效区分,对疾病标志物研究提出很大挑战。本研究以亲水相互作用色谱法(HILIC)为基础,对该富集方法中活化、清洗与洗脱过程进行优化,其中主要对HILIC填料粒径和富集缓冲体系进行优化,并考察了不同实验条件下N-糖肽富集的鉴定数量、选择性与稳定性,发...

Extracellular vesicles (EVs) are nanoscale vesicles with a phospholipid bilayer. In the past few decades, EVs have gained more and more attention, which is attributed to their important roles in cell-to-cell communication. They are regarded as promising sources for disease biomarkers and have been explored for applications in early-stage diagnostic...

Exosomes are small membrane-bound vesicles secreted by most cell types and play an important role in cell-to-cell communication. The increasing evidence shows that exosomal proteins in urine may be used...

Microbial adaptation to environmental stress involves complex adaptations of bacteria. Many such responses are transient and dynamic. However, monitoring the dynamic responses of live bacteria to stimulations at the molecular level remain a challenge. This work describes the development of syringe spray mass spectrometry (MS) method that allows dir...

Previously, we have reported the development of a miniature mass spectrometer with a continuous atmospheric pressure interface, whose sensitivity was sacrificed due to the limited gas flow rate. In this study, a circular lens was designed and integrated on the ion transfer pathway between the two pumping stages, and a ten-fold sensitivity improveme...

Most electrospray based ambient ionization techniques, e.g., laser ablation electrospray ionization (LAESI), utilize a fixed spray solution composition. Complex samples often contain compounds of different polarity that exhibit a wide range of solubilities in the electrospray solvent. Thus, the fixed spray solution composition limits the molecular...

Visualizing the interaction zoneRapidly emerging microbial resistance to antibiotics is a growing concern for the treatment of infectious diseases. In their Communication on page 15035 ff., A. Vertes et al. describe the application of laser ablation electrospray ionization (LAESI) in combination with mass spectrometry (MS) to gain deeper molecular...

Die Resistenzentwicklung von Mikroben gegen Antibiotika ist ein ernstes Problem bei der Behandlung von Infektionskrankheiten. In ihrer Zuschrift (DOI: 10.1002/ange.201607751) beschreiben A. Vertes et al. die Anwendung von Laserablation mit Elektrosprayionisation (LAESI) in Kombination mit Massenspektrometrie (MS), um molekulare Einblicke in die Wec...

Metabolism in microbial colonies responds to competing species, rapidly evolving genetic makeup, and sometimes dramatic environmental changes. Conventional characterization of the existing and emerging microbial strains and their interactions with antimicrobial agents, e.g., the Kirby–Bauer susceptibility test, relies on time consuming methods with...

Metabolism in microbial colonies responds to competing species, rapidly evolving genetic makeup, and sometimes dramatic environmental changes. Conventional characterization of the existing and emerging microbial strains and their interactions with antimicrobial agents, e.g., the Kirby–Bauer susceptibility test, relies on time consuming methods with...

Native electrospray ionization (ESI) mass spectrometry (MS) is often used to monitor noncovalent complex formation between peptides and ligands. The relatively low throughput of this technique, however, is not compatible with extensive screening. Laser ablation electrospray ionization (LAESI) MS combined with ion mobility separation (IMS) can analy...

Mass spectrometry imaging (MSI) is an emerging technology for the mapping of molecular distributions in tissues. In most of the existing studies, imaging is performed by sampling on a predefined rectangular grid that does not reflect the natural cellular pattern of the tissue. Delivering laser pulses by a sharpened optical fiber in laser ablation e...

Mass spectrometry imaging (MSI) by laser ablation electrospray ionization (LAESI) enables the lateral mapping of molecular distributions in untreated biological tissues. However, direct sampling and ionization by LAESI-MSI limits the differentiation of isobaric ions (e.g., structural isomers) in a complex sample. Ion mobility separation (IMS) of LA...

Applications of laser ablation electrospray ionization mass spectrometry (LAESI-MS) have been widely explored in positive ion mode. This technique enables the direct detection of compounds with a propensity for positive ion formation. However, many important compounds, e.g., organic acids, tend to more readily deprotonate and produce negative ions....

Islet amyloid polypeptide (amylin) is a 37-residue peptide that is co-secreted with insulin. Studies suggest that amylin can aggregate and contribute to type 2 diabetes mellitus by inducing pancreatic beta-cell death. The inhibitory effect of copper(II) on amylin aggregation has been recently discovered but little is known about the details of the...