Hanadi F Sleiman

Hanadi F Sleiman
McGill University | McGill · Department of Chemistry

Ph.D.

About

196
Publications
27,071
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8,856
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January 2003 - December 2012
McGill University

Publications

Publications (196)
Article
Full-text available
The self-assembly of block copolymers is often rationalized by structure and microphase separation; pathways that diverge from this parameter space may provide new mechanisms of polymer assembly. Here, we show that the sequence and length of single-stranded DNA directly influence the self-assembly of sequence-defined DNA block copolymers. While inc...
Preprint
Nanoparticle (NP) ripening is a process where energetically favored larger particles grow at the expense of smaller ones: while important in NP synthesis, it is often difficult to control during NP applications. Here, we unveil a new Contact-dependent, Localized Galvanic Ripening (CLGR) mechanism that enables precise control of NP ripening in solut...
Preprint
Nanoparticle (NP) ripening is a process where energetically favored larger 8 particles grow at the expense of smaller ones: while important in NP synthesis, it is often difficult to control during NP applications. Here, we unveil a new Contact-dependent, Localized Galvanic Ripening (CLGR) mechanism that enables precise control of NP ripening in sol...
Article
Supramolecular chemistry involves the noncovalent assembly of monomers into materials with unique properties and wide-ranging applications. Thermal analysis is a key analytical tool in this field, as it provides quantitative thermodynamic information on both the structural stability and nature of the underlying molecular interactions. However, ther...
Preprint
Polymers with sequence definition allow access to programmable morphologies and applications, but directly correlating polymer structure to function currently requires case-by-case analysis: high throughput methods that identify promising species from entire chemical families are required. Here, we show that the discovery of effective protein targe...
Preprint
Full-text available
Supramolecular chemistry involves the non-covalent assembly of monomers into materials with unique properties and wide-ranging applications. Thermal analysis is a key analytical tool in this field, as it provides quantitative thermodynamic information on both the structural stability and nature of the underlying molecular interactions. However ther...
Preprint
Supramolecular chemistry involves the non-covalent assembly of monomers into materials with unique properties and wide-ranging applications. Thermal analysis is a key analytical tool in this field, as it provides quantitative thermodynamic information on both the structural stability and nature of the underlying molecular interactions. However ther...
Article
Full-text available
Biochemical networks interconnect, grow and evolve to express new properties as different chemical pathways are selected during a continuous cycle of energy consumption and transformation. In contrast, synthetic systems that push away from equilibrium usually return to the same self-assembled state, often generating waste that limits system recycla...
Preprint
Full-text available
Supramolecular chemistry involves the non-covalent assembly of monomers into materials with unique properties and wide-ranging applications. Thermal analysis is a key analytical tool in this field, as it provides quantitative thermodynamic information on both the structural stability and nature of the underlying molecular interactions. However ther...
Preprint
Supramolecular chemistry involves the non-covalent assembly of monomers into materials with unique properties and wide-ranging applications. Thermal analysis is a key analytical tool in this field, as it provides quantitative thermodynamic information on both the structural stability and nature of the underlying molecular interactions. However ther...
Article
DNA nanostructures are highly addressable and compatible with biological systems but often require hundreds of unique strands for their assembly. On the other hand, nature can assemble complex structures from identical building blocks by compartmentalizing the process: assembling molecules into sub-components and bringing these together across mult...
Article
DNA nanotechnology has produced a wide range of self-assembled structures, offering unmatched possibilities in terms of structural design. Because of their programmable assembly and precise control of size, shape, and function, DNA particles can be used for numerous biological applications, including imaging, sensing, and drug delivery. While the b...
Article
Full-text available
Drug delivery vectors for nucleic acid therapeutics (NATs) face significant barriers for translation into the clinic. Spherical nucleic acids (SNAs) – nanoparticles with an exterior shell made up of DNA strands and a hydrophobic interior – have recently shown great potential as vehicles to improve the biodistribution and efficacy of NATs. To date,...
Article
Full-text available
DNA tweezers have emerged as powerful devices for a wide range of biochemical and sensing applications; however, most DNA tweezers consist of single units activated by DNA recognition, limiting their...
Article
Full-text available
The diversity of DNA duplex structures is limited by a binary pair of hydrogen-bonded motifs. Here we show that poly(thymine) self-associates into antiparallel, right-handed duplexes in the presence of melamine, a small molecule that presents a triplicate set of the hydrogen-bonding face of adenine. X-ray crystallography shows that in the complex t...
Article
Full-text available
Single molecules can now be visualised with unprecedented precision. As the resolution of single-molecule experiments improves, so too does the breadth, quantity and quality of information that can be extracted using these methodologies. In the field of DNA nanotechnology, we use programmable interactions between nucleic acids to generate complex,...
Article
DNA nanotechnology has generated a wealth of structures uniquely suited for nanoscale patterning; however, scalability, affordability, and recyclability are important preconditions for the industrial production and widespread use of DNA-based materials. In this perspective, we propose that “printing” programmed particles from transient DNA template...
Article
Nickel is a highly important metal, and the detection of Ni²⁺ using biosensors is a long-stand analytical challenge. DNA has been widely used for metal detection, although no DNA-based sensors were reported for Ni²⁺. DNAzymes are DNA-based catalysts, and they typically recruit metal ions for catalysis. In this work, in vitro selection of RNA-cleavi...
Article
Full-text available
The construction of metallic nanostructures with customizable morphologies and complex shapes has been an essential pursuit in nanoscience. DNA nanotechnology has enabled the fabrication of increasingly complex DNA nanostructures with unprecedented specificity, programmability and sub-nanometer precision, which makes it an ideal chaperone to ration...
Article
The self‐immolative, amplified release of small molecules, in response to a nucleic‐acid signal, from 1D, 2D, and 3D assemblies using the hybridization chain reaction (HCR) is demonstrated. These assemblies effectively separate the reactive groups, enabling the release of small molecules in response to a biomarker of interest, and could find applic...
Article
Triggering the release of small molecules in response to unique biomarkers is important for applications in drug delivery and biodetection. Due to typically low quantities of biomarker, amplifying release is necessary to gain appreciable responses. Nucleic acids have been used for both their biomarker recognition properties and as stimuli, notably...
Article
Conjugation of lipid moieties to nucleic-acid therapeutics increases their interaction with cellular membranes, enhances their uptake and influences in vivo distribution. Once injected in biological fluids, such modifications trigger the binding of various serum proteins, which in turn play a major role in determining the fate of oligonucleotides....
Chapter
DNA nanotechnology has been used to create DNA containing nanostructures with well-defined sizes and shapes—properties highly applicable to drug delivery. By appending sequence-defined hydrophobic segments to DNA, DNA amphiphiles are created whose structures and modes of self-assembly mimic specialized biomacromolecules such as proteins. Automated,...
Article
In der DNA-Nanotechnologie wird häufig ein Double-Crossover-Motiv verwendet, um Architekturen zu versteifen und die DNA-Konnektivität zu verbessern. Die Funktionalisierung dieser Kacheln mit Metallkomplexen an den Kreuzungspunkten erhöht die thermische Stabilität im Vergleich zu den nicht funktionalisierten Derivaten. Abstract The double crossover...
Article
Full-text available
The double crossover junction (DX) is a fundamental building block for generating complex and varied structures from DNA. However, its implementation in functional devices is limited to the inherent properties of DNA itself. Here, we develop design strategies to generate the first metal-DX DNA tiles (DX M ), by site-specifically functionalizing the...
Chapter
During the past 30 years, the field of DNA nanotechnology has witnessed significant development and has been applied in various other fields such as biology, physics, chemistry, and engineering [1-3]. The number of laboratories working on this topic has expanded immensely, leading to the generation of a variety of two- (2D) and three-dimensional (3...
Article
Highly selective recognition of metal ions by rational ligand design is challenging, and simple metal binding by biological ligands is often obscured by nonspecific interactions. In this work, we used binding‐triggered catalysis, where metal selectivity steeply increased by increasing the number of metal ions involved, as exemplified in a series of...
Article
Highly selective recognition of metal ions by rational ligand design is challenging, and simple metal binding by biological ligands is often obscured by nonspecific interactions. In this work, we used binding‐triggered catalysis, where metal selectivity steeply increased by increasing the number of metal ions involved, as exemplified in a series of...
Article
Full-text available
DNA nanotechnology relies on the molecular recognition properties of DNA to produce complex architectures through self-assembly. The resulting DNA nanostructures allow scientists to organize functional materials at the nanoscale and have therefore found applications in many domains of materials science over the past several years. These scaffolds h...
Article
An approach to electrogenerated chemiluminescence (ECL) signal enhancement, utilizing the highly tailorable ring opening metathesis polymerization (ROMP) to generate novel ECL active nanoscale structures is explored. The strategy involves integrating into a single polymer multiple copies of an analogue of the established ECL luminophore, tris(2,2’‐...
Article
Full-text available
Herein we present a new strategy to achieve chiral induction and redox switching along the backbone of metallohelicate architectures, wherein a DNA duplex directs the handedness and charge transport properties of a metal-organic assembly more than 60 bonds away (a distance of >10 nm). The quantitative and site-specific binding of copper(I) ions to...
Chapter
DNA nanotechnology enables the design and assembly of DNA nanostructures with unprecedented control over their size and shape. Additionally, the programmable base-pairing alphabet of DNA allows the incorporation of responsive units within these DNA nanostructures. Here, we describe a general design strategy to construct responsive DNA prisms that c...
Article
Full-text available
Fluorescent dye labeling of DNA oligonucleotides and nanostructures is one of the most used techniques to track their fate and cellular localization inside cells. Here, we report that intracellular fluorescence, and even FRET signals, cannot be correlated with the cellular uptake of intact DNA structures. Live cell imaging revealed high colocalizat...
Article
To mark the occasion of Nature Chemistry turning 10 years old, we asked scientists working in different areas of chemistry to tell us what they thought the most exciting, interesting or challenging aspects related to the development of their main field of research will be — here is what they said.
Preprint
p> Natural biopolymers achieve information storage, molecular recognition and catalysis efficiently through sequence-control. To be able to mimic such properties, self-assembly studies of artificial sequence-defined oligomers is of great interest. In this paper, we show the use of hydrophilic, lipophilic, aromatic and fluorophilic monomers to synth...
Article
Full-text available
In this work, we report a component-minimal spherical nucleic acid (SNA) from monodisperse DNA-polymer conjugates that can load and release nucleic acid therapeutics in a stimuli-responsive manner. We show that this vehicle assembles from only four strands, and conditional release of its antisense therapeutic cargo can be induced upon recognition o...
Article
Cells use membrane proteins as gatekeepers to transport ions and molecules, catalyze reactions, relay signals, and interact with other cells. DNA nanostructures with lipidic anchors are promising as membrane protein mimics because of their high tuneability. However, the design features specifying DNA nanostructure’s functions in lipid membranes are...
Article
Full-text available
Dynamic wireframe DNA structures have gained significant attention in recent years, with research aimed towards using these architectures for sensing and encapsulation applications. For these assemblies to reach their full potential, however, knowledge on the rates of strand displacement and hybridization on these constructs is required. Herein, we...
Article
Full-text available
The incorporation of synthetic molecules as corner units in DNA structures has been of interest over the last two decades. In this work, we present a facile method to generate branched small molecule‐DNA hybrids with controllable valency, different sequences and directionalities (5’‐3’) using a “printing” process from a simple 3‐way junction struct...
Article
The incorporation of synthetic molecules as corner units in DNA structures has been of interest over the last two decades. In this work, we present a facile method to generate branched small molecule‐DNA hybrids with controllable valency, different sequences and directionalities (5’‐3’) using a “printing” process from a simple 3‐way junction struct...
Article
RNA has inherent therapeutic and structural properties that make it an important component of biologically-functional nanoparticles. Using DNA-amphiphiles as synthetic templates, we report the synthesis of two classes of RNA-amphiphiles...
Article
Sequence-defined polymers with customizable sequences, monodispersity, substantial length, and large chemical diversity are of great interest to mimic the efficiency and selectivity of biopolymers. We report an efficient, facile, and scalable synthetic route to introduce many chemical functionalities, such as amino acids and sugars in nucleic acids...
Article
Full-text available
Understanding how biological macromolecules assemble into higher-order structures is critical to explaining their function in living organisms and engineered biomaterials. Transient, partly-structured intermediates are essential in many assembly processes and pathway selection, but are challenging to characterize. Here we present a simple thermal h...
Article
Supramolecular one-dimensional (1D) architectures are of high interest in drug delivery and templation of complex linear arrays due to their high aspect ratio and rigidity. A particular desire is the access of 1D nanostructures with high functionality and bio-relevance, which opens the door to a wide variety of their applications in materials scien...
Article
DNA origami is one of the most effective tools for bottom-up construction of novel objects and devices at the nanometer-scale. However, many applications require larger structures than can be obtained with the conventional single-stranded scaffold, typically 7249 nucleotides. Here, we address this limitation by developing custom-made single-strande...
Article
DNA is a powerful guiding molecule to achieve the precise construction of arbitrary structures and high-resolution organization of functional materials. The combination of sequence programmability, rigidity and highly specific molecular recognition in this molecule has resulted in a wide range of exquisitely designed DNA frameworks. To date, the im...
Article
Over the past four decades, the field of structural DNA nanotechnology has evolved into an accessible approach to generating DNA nanostructures with precise control of size, geometry, and presentation of ligands. Moreover, the biocompatibility of DNA, its programmability, responsiveness to biological cues, ready synthesis, and ease of functionaliza...
Article
Full-text available
As colloidal self-assembly increasingly approaches the complexity of natural systems, an ongoing challenge is to generate non-centrosymmetric structures. For example, patchy, Janus or living crystallization particles have significantly advanced the area of polymer assembly. It has remained difficult, however, to devise polymer particles that associ...
Article
Natural systems combine different supramolecular interactions in a hierarchical manner to build structures. In contrast, DNA nanotechnology relies almost exclusively on DNA base pairing for structure generation. Introducing other supramolecular interactions can expand the structural and functional range of DNA assemblies, but this requires an under...
Chapter
Full-text available
Deoxyribonucleic acid (DNA) has evolved in nature to store and transfer the genetic information of most life on earth. The fidelity of information processing relies on the precise pairing through noncovalent interactions of a molecular code consisting of four unique nucleobases. By using this programmability DNA can be taken out of its biological c...
Article
Gold nanoparticles (AuNPs) endowed with anisotropic DNA valency are an important class of materials, as they can assemble into complex structures with a minimal number of DNA strands. However, methods to encode 3D DNA strand patterns on AuNPs with a controlled number of unique DNA strands in a predesigned spatial arrangement remain elusive. In this...
Article
Full-text available
DNA is the molecule that stores and transmits genetic information in biological systems. The field of DNA nanotechnology takes this molecule out of its biological context and uses its information to assemble structural motifs and then to connect them together. This field has had a remarkable impact on nanoscience and nanotechnology, and has been re...
Article
We present a rapid and efficient method to generate a family of platinum supramolecular square complexes, including previously inaccessible targets, through the use of ball milling mechanochemistry. This one-pot, two-step process occurs in minutes and enables the synthesis of the squares [Pt4(en)4(N∩N)4][CF3SO3]8 (en= ethylenediamine, N∩N = 4,4’-bi...
Conference Paper
Full-text available
Electrogenerated chemiluminescence (ECL) is an electron transfer between redox products formed at an electrode that results in the formation of an excited state species, which is capable of photon emission. This excited state can be achieved by a reaction between an oxidized and a reduced form of the same luminophore, or via the reaction of the oxi...
Article
Full-text available
Long-range ordering of DNA crossover tiles with blunt ends on lipid bilayers is investigated using atomic force microscopy. “Blunt-ended” tiles do not have single-stranded complementary ends, and thus instead of assembling via base-pairing, they can interact by π-stacking of their duplex ends. This work demonstrates that the balance of base π-stack...
Article
Full-text available
A wide variety of approaches have become available for fabricating nanomaterials with increasing degrees of complexity, precision, and speed while minimizing cost. Their quantitative characterization however remains a challenge. Analytical methods to better inspect and validate the structure/composition of large nanoscale objects are required to op...
Article
Full-text available
We report a spherical nucleic acid (SNA) system for the delivery of BKM120, an anticancer drug for treatment of chronic lymphocytic leukemia (CLL). While promising for cancer treatment, this drug crosses the blood-brain barrier causing significant side-effects in patients. The DNA nanoparticle encapsulates BKM120 in high efficiency, and is unparall...
Article
Herein, we validate a novel cuvette-based approach to measuring electrogenerated chemiluminescence (ECL) employing the hyphenation of a commercial spectrometer and potentiostat. The new experimental design provides simultaneous acquisition of electrochemical and ECL data allowing complementary analysis by both techniques. Preliminary analysis of an...
Chapter
Full-text available
One important emerging field is the use of synthetic molecules to tune and modify the stability, functionality, and assembly of DNA-based structures. These molecules can be incorporated into the structures through one of two methods: covalent insertion or non-covalent interactions. Due to the ease of automated synthesis and the efficiency of coupli...
Article
Full-text available
The development of nucleic acid therapeutics has been hampered by issues associated with their stability and in vivo delivery. To address these challenges, we describe a new strategy to engineer DNA structures with strong binding affinity to Human Serum Albumin (HSA). HSA is the most abundant protein in the blood and has a long circulation half-lif...
Article
Full-text available
We set out to design, synthesize, and optimize a DNA-minimal cage capable of encapsulating oligonucleotide drugs to facilitate their delivery. Through rational design and optimization using in vitro assays, we have assembled the first DNA "nanosuitcase" that can encapsulate a siRNA construct and release it upon recognition of an oligonucleotide tri...
Article
We develop a versatile recognition system based on 3D triangular-shaped DNA nanotubes by integrating three different aptamer sequences along the three edges. This would allow multiple binding activities to be combined into a single system. The versatility of this nanotube platform can also provide a framework for spatial orientation and positioning...
Article
Full-text available
Perfluorocarbons (PFCs) are a promising class of molecules for medical applications: they are detectable through ¹⁹F nuclear magnetic resonance (NMR) and they assemble separately from water or lipophilic phases, thus bringing unique supramolecular interactions into nanostructures. We report the ready insertion of PFCs into nucleic acids, as well as...
Article
Full-text available
We report a micelle-templated method to enhance the reactivity of DNA with highly hydrophobic molecules. Lipids, chromophores and polymers can be conjugated to DNA in high yield and under mild conditions. This method expands the range of DNA-templated reactions for DNA-encoded libraries, oligonucleotide and drug delivery, nanopore mimetics and DNA...