Hana Malinska

Jan Evangelista Purkyně University | UJEP · Department of Biology

Plant extracellular vesicles (pEVs) derived from numerous edible sources gain a lot of attention in recent years, mainly due to the potential to efficiently carry bioactive molecules into mammalian cells. In the present study, we focus on isolation of PDNVs (plant-derived nanovesicles) and pEVs from callus culture and from BY-2 culture of Nicotiana...

Microbial community of the soil can be subdivided into a planktonic community of motile organisms and a community of sessile organisms, including those constituting biofilms on physical surfaces of different texture. Biofilm communities on surfaces of organic particles represent a crucial factor involved in decomposition of plant litter and soil pa...

The second generation energy crop Miscanthus x giganteus is considered as one of the most perspective crops for biomass production in marginal sites with undesirable soil parameters. An experiment in large pots was established to compare the effect of cultivation in sandy soil with nutrients deficiency and soil contaminated by multiple risk element...

Extracellular vesicles are small membrane particles (30-1000 nm) released by Bacteria, Eukaryotes and Archaea. They have been shown to play an important role in intracellular and intercellular communication, within and between kingdoms via transport of bioactive molecules. Thus, they can be involved in altering gene expression and regulation of phy...

Despite extensive study of extracellular vesicles (EVs), specifically exosomes (EXs) as biomarkers, important modulators of physiological or pathological processes, or therapeutic agents, relatively little is known about nonconventional sources of EXs, such as invertebrate or plant EXs, and their uses. Likewise, there is no clear information on the...

During the last couple of years, the traditionally mild climate of central Europe changed to very hot and dry (including the spring season). Local plants and crops need to deal with this stressful change causing a yield decrease. Primary aims were to explore simple ways of "hardening" Miscanthus x giganteus J.M.Greef, Deuter ex Hodk., Renvoize ener...

Second-generation biofuel crop miscanthus is one of the most promising plants tested for phytomanagement of contaminated sites. In this preliminary pot case study, the most used hybrid Miscanthus x giganteus was cultivated in three different real contaminated soils: agricultural soil contaminated with Cd; post-military soil slightly contaminated wi...

Bioenergy crops such as Miscanthus x giganteus are foreseeable as an alternative source to replace fossil fuel and reduce greenhouse gas emissions. They are also assessed as an environment-friendly solution for polluted, marginal and low-quality agricultural soils. Several studies had been launched on soil organic carbon sequestration potentials of...

Most of the heavy metals (HMs), and metals/metalloids are released into the nature either by natural phenomenon or anthropogenic activities. Being sessile organisms, plants are constantly exposed to HMs in the environment. The metal nonhyperaccumulating plants are susceptible to excess metal concentrations. They tend to sequester metals in their ro...

Use of plant growth-promoting bacteria (PGPB) for cultivation of the biofuel crop Miscanthus × giganteus (Mxg) in post-military and post-mining sites is a promising approach for the bioremediation of soils contaminated by metals. In the present study, PGPB were isolated from contaminated soil and screened for tolerance against abiotic stresses caus...

Sex determination and sex chromosomes have been extensively studied due to their importance in evolutionary biology. Species of dioecious plants, which may have sex chromosomes, offer us unique insights into the history of this phenomenon across the Tree of Life. Here, we present Sex‐chrom: a database on plant sex chromosomes (www.sexchrom.csic.es)...

Miscanthus x giganteus (Mxg) is a promising second-generation biofuel crop with high production of energetic biomass. Our aim was to determine the level of plant stress of Mxg grown in poor quality soils using non-invasive physiological parameters and to test whether the stress could be reduced by application of plant growth regulators (PGRs). Plan...

Background: To determine the level of plant stress of second-generation biofuel crop Miscanthus x giganteus ( Mxg ) grown in poor quality post-military soils using physiological parameters; to identify impact of plant growth regulators (PGRs) on the stress reduction and to select the fluorescence indicators most suitable for quantification of stres...

The impact of plant growth regulators (PGRs) “Stimpo” and “Regoplant” on Miscanthus x giganteus ( Mxg ) biomass parameters was investigated when the plant was grown in military soils with different properties from Dolyna, Ukraine and Hradcany, Czech Republic. The results showed that PGRs positively influenced the biomass parameters when the plant w...

To study the relationship between uniparental rDNA (encoding 18S, 5.8S and 26S ribosomal RNA) silencing (nucleolar dominance) and rRNA gene dosage, we studied a recently emerged (within the last 80 years) allotetraploid Tragopogon mirus (2n=24), formed from the diploid progenitors T. dubius (2n=12, D-genome donor) and T. porrifolius (2n=12, P-genom...

Tragopogon mirus Ownbey and Tragopogon miscellus Ownbey are recent allotetraploids that have formed recurrently within the last 80 years, following the introduction of the diploids T. dubius Scop., T. pratensis L. and T. porrifolius L. from Europe to North America. In some areas, the progenitor diploids still occur along with expanding populations...

Southern blot analysis of the S2 generation of synthetic T. mirus. Individuals were the progenies of three lineages from line 73.

Analysis of intergenic rDNA spacer polymorphisms in DNA of synthetic T. mirus (S1 generation). Genomic DNA was digested with BstYI and SspI restriction enzymes. Southern blot hybridization was carried out using the 26S rDNA probe.

Analysis of IGS subrepeats in T. dubius 2613 and T. porrifolius 2611. We used a dot plot alignment tool at http://www.vivo.colostate.edu/molkit/dnadot/, self (x-axis) to self (y-axis) alignment (Window size: 9. Mismatch limit: 0). The IGSs were amplified using primers designed to conserved regions in 26S rDNA and 18S rDNA [10]. Briefly, the ~3.5-kb...

Slot blot quantification of gene copies in parental diploids. The DNA amounts are indicated above each lane. The blot was hybridized with the 32P - labeled 18S rDNA probe. Experiments I and II were carried out in this study; experiment III is from [45].

FISH analysis of the S2 generation of synthetic T. mirus. The same plants as in Additional file 3 were analyzed. Most metaphases displayed aneuploid karyotypes (23 chromosomes). Arrowheads in (C, F) indicate a minute Dpo locus left after the deletion of the majority of genes. Note fusion of subtelomeric NORs at the chromatids (arrowheads, E) and co...

Expression analysis of rDNA in synthetic T. mirus (line 116). RNA isolation and RT-CAPS assay were carried out as described in [60]. Note typical inverse correlation between gene copy number (grey bars) and their expression (black bars).

Tragopogon mirus and T. miscellus are allotetraploids (2n = 24) that formed repeatedly during the past 80 years in eastern Washington and adjacent Idaho (USA) following the introduction of the diploids T. dubius, T. porrifolius, and T. pratensis (2n = 12) from Europe. In most natural populations of T. mirus and T. miscellus, there are far fewer 35S...

Background: Polyploidy, frequently termed "whole genome duplication", is a major force in the evolution of many eukaryotes. Indeed, most angiosperm species have undergone at least one round of polyploidy in their evolutionary history. Despite enormous progress in our understanding of many aspects of polyploidy, we essentially have no information a...

We analyzed nuclear ribosomal DNA (rDNA) transcription and chromatin condensation in individuals from several populations of Tragopogon mirus and T. miscellus, allotetraploids that have formed repeatedly within only the last 80 years from T. dubius and T. porrifolius and T. dubius and T. pratensis, respectively. We identified populations with no (2...