Hana Greifová

Slovak University of Agriculture in Nitra - Slovenska posnohospodarska univerzita v Nitre · Department of Animal Physiology (KFŽ)

The aim of the study was to examine the potential impacts of bisphenol A (BPA) and its analogues BPB, BPF, and BPS on mice TM3 Leydig cells, with respect to basal cell viability parameters such as metabolic activity, cell membrane integrity, and lysosomal activity after 48-h exposure. In addition, monitoring of potential bisphenol´s actions include...

Endocrine-disrupting chemicals (EDCs) have recently attracted the interest of public health organizations, motivating substantial research to identify their effect on human health. Dysfunctions in endocrine system activities can raise the risk of a wide range of disorders, including cardiovascular disease, type 2 diabetes, cancer, and reproductive...

Tribulus terrestris L. is an herbaceous plant native to India, widely used as a natural sexual stimulant by traditional medicine in China, India, and Greece, and also for the treatment of various diseases for example low blood pressure, cardiovascular diseases, kidney, and skin diseases, etc. Tribulus extracts are rich in numerous biologically acti...

The prevalence of reproductive dysfunction in males has risen in the last few years, and alternative therapies are gradually gaining in popularity. Our in vitro study aimed to evaluate the potential impact of Lepidium sativum L. on mice TM3 Leydig cells, concerning basal parameters such as cell viability, cell membrane integrity, and lysosomal acti...

Many studies have revealed that oxidative stress is a primary factor in the pathogenesis of male reproductive system dysfunctions. The strong antioxidant and cytoprotective effects of resveratrol have previously been demonstrated, but its effect in the context of the male reproduction remains unconvincing. To observe the biological activity of resv...

The alarming growth of chronic diseases is a major problem in Europe, and current data suggest an even greater burden in the future. Although genetic predispositions remain important determinants in the development of certain disorders, the appropriate diet can considerably minimize the risk of many diseases. Many plant species do, in fact, have he...

In December of 2019, several cases of unknown atypical respiratory diseases emerged in Wuhan, Hubei Province in China. After preliminary research, it was stated that the disease is transmittable between humans and was named COVID-19. Over the course of next months, it spread all over the world by air and sea transport and caused a global pandemic w...

The inflammatory reaction accompanies in part or in full any disease process in the vascularized metazoan. This complicated reaction is controlled by regulatory mechanisms, some of which produce unpleasant symptomatic manifestations of inflammation. Therefore, there has been an effort to develop selective drugs aimed at removing pain, fever, or swe...

In recent years, bisphenol analogues such as bisphenol B (BPB), bisphenol F (BPF), and bisphenol S (BPS) have come to replace bisphenol A (BPA) in food packaging and food containers, since BPA has been shown to leach into food and water, causing numerous negative health effects. Although much information on the endocrine activity of BPA is availabl...

In this study, we evaluated the in vitro effects of 1-50 μM zearalenone (ZEA), deoxynivalenol (DON) and T-2 toxin (T-2) on rabbit spermatozoa for as much as 8 h of in vitro exposure. Our results indicate that all sperm quality parameters were negatively affected by these fusariotoxins in a time- and dose-dependent manner. The most prominent structu...

Several plants have the potential to protect essential reproductive processes such as spermatogenesis or steroidogenesis, however, effective concentrations and main mechanisms of action are still unknown. This in vitro study was aimed to assess the effects of Apium graveolens L., Levisticum officinale, and Calendula officinalis L. extracts on the s...

Natural processes along with increased industrial production and the irresponsible behavior of mankind have resulted in environmental pollution. Environmental pollutants can be categorized based on their characteristics and appearance into the following groups: physical, biological, and chemical. Every single one of them represents a serious threat...

Bisphenol A (BPA) is the most well-known compound from the bisphenol family. There is increasing evidence that bisphenol BPA used in plastics, receipts, food packaging, and other products might be harmful to human health due to its actions as an endocrine-disrupting chemical, therefore BPA is being replaced by compounds very similar in structure, b...

This study was focused to determine an individual and combined effect of mycotoxin citrinin (CIT) and two compounds of the stilbene family- resveratrol (RES) and his dimethyl ether analogue pterostilbene (PTE) which have many health benefits. As a model the human adenocarcinoma cell line HT-29 was used which may exhibits the properties of small int...

The aim of our study was to reveal the in vitro effects of Salvia officinalis L. (37.5, 75, 150, 200, 250, 300 and 600 µg/ml) extract on the TM3 Leydig cell viability, membrane integrity, steroidogenesis and reactive oxygen species production after 24 h and 48 h cultivation. For the present study, the extract prepared from Salvia officinalis L. lea...

The objective of present study was to investigate in vitro protective potential of resveratrol in TM3 Leydig cells with induced oxidative stress using hydrogen peroxide (H2O2). Leydig cells experiencing oxidative stress exhibit reduced activities in androgens production, and become hypofunctional with age, which is also related to growing oxidative...

The increasing worldwide production of bisphenols has been associated to several human diseases, such as chronic respiratory and kidney diseases, diabetes, breast cancer, prostate cancer, behavioral troubles and reproductive disorders in both sexes. The aim of the present in vitro study was to evaluate the potential impact bisphenols A, B, S and F...

The aim of this study was to evaluate the dose- and time-dependent in vitroeffects of isoquercitrin (ISO), a natural flavonoid with numerous biological properties on bovine spermatozoa during three different time periods (0 h, 2 h, 24 h). Bovine semen samples were diluted and cultivated in physiological saline solution containing 0.5% DMSO together...

The aim of our in vitro study was to assess the potential effect of 4-octylphenol (4-OP) on the basal and human chorionic gonadotropin (hCG)-stimulated cholesterol levels and biosynthesis of steroid hormones in cultured mouse Leydig cells. In addition, we evaluated the intracellular superoxide production following 4-OP treatment. Isolated mouse Ley...

Evidence shows that oxidative stress associated with sperm cryopreservation may lead to a significant decrease of the structural integrity and functional activity of male gametes. Curcumin (CUR) has become a substance of scientific interest for its free radical-quenching abilities, which could enhance the post-thaw quality of male gametes. This stu...

Over the last decade, there is growing incidence of male reproductive malfunctions. It has been documented that numerous environmental contaminants, such as endocrine disruptors (EDs) may adversely affect the reproductive functions of humans as well as wildlife species. The aim of this in vitro study was to examine the effects of 4-octylphenol (4-O...

To preserve and protect spermatozoa against any possible oxidative damage the addition of natural antioxidants might be the ideal solution which has been investigated worldwide. The composition of the diluents is as follows: 50 μM/L vitamin C with 0.5% DMSO (dimethyl sulfoxide, Sigma, St. Louis, USA); 50 μM/L vitamin E with 0.5% ethanol; each added...

The purpose of this study was to determine the effect of epicatechin (EPI) on the motility, viability, and reactive oxygen species (ROS) production of bovine spermatozoa in the presence of various EPI doses during different time periods (Time 0 h, 2 h, 6 h, 24 h). Semen samples were cultivated in physiological saline solution containing 200, 100, 5...

Reactive oxygen species overgeneration as a side effect of semen cryopreservation may lead to lipid peroxidation, protein degradation, DNA fragmentation and cell death, resulting in a decrease of sperm survival and fertilisation ability. Lycopene has been proposed as a potential supplement to semen extenders because of its antioxidant properties. T...

In the present study, isolated interstitial (Leydig) cells from testes of male mice and H295R cells (human adrenocarcinoma cell line) were used as an in vitro model for detection toxic effects of 4-NP at various concentrations (0.04; 0.2; 1.0; 2.5 and 5.0 µg/mL) on the androstenedione and testosterone production. The steroide hormone production wer...