Han Yih Lau

Han Yih Lau
Malaysian Agricultural Research and Development Institute (MARDI) | mardi · Biotechnology Research Centre (BT)

Doctor of Philosophy

About

25
Publications
11,315
Reads
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433
Citations
Citations since 2016
19 Research Items
418 Citations
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2016201720182019202020212022020406080100
2016201720182019202020212022020406080100

Publications

Publications (25)
Article
Full-text available
More than 200 different cultivars of durian exist worldwide but Durio zibethinus or Musang King (MK) is the most premium and prized durian fruit among the recommended varieties. Early identification of this premium variety is critical to protect from non-authentic MK durian cultivars. However, the MK variety's morphological traits are nearly identi...
Article
Full-text available
Halal authentication has become essential in the food industry to ensure food is free from any prohibited ingredients according to Islamic law. Diversification of food origin and adulteration issues have raised concerns among Muslim consumers. Therefore, verification of food constituents and their quality is paramount. From conventional methods bas...
Article
Full-text available
Bananas are one of the most crucial fruit crops worldwide and significantly contribute to food security in developing countries. However, blood disease of bananas caused by Ralstonia syzygii subspecies celebensensis has become a threat to banana production. Rapid and accurate diagnosis of BDB for on-site detection is pivotal at an early stage for a...
Article
Full-text available
Plant diseases caused by numerous pathogens such as bacteria, viruses, and fungi are responsible for substantial economic losses in the agricultural industry worldwide. Specific, sensitive, and efficient diagnostic tools have been developed worldwide to mitigate and prevent the pathogenic threat. The diagnostic tools have revolutionized from classi...
Article
Full-text available
For centuries, rice has been the one of the most important crops worldwide; over 3.5 billion of the population relies on rice as one of the most essential staple foods. However, rice diseases are responsible for enormous global economic losses due to the damage they cause to rice crops. Major rice diseases such as bacterial leaf blight (BLB), rice...
Article
Full-text available
Over the years, development of molecular diagnostics has evolved significantly in the detection of pathogens within humans and their surroundings. Researchers have discovered new species and strains of viruses, while mitigating the viral infections that occur, owing to the accessibility of nucleic acid screening methods such as polymerase chain rea...
Article
Full-text available
Identification of plant variety and cultivar is pivotal in the agricultural sector due to the abundance of plant varieties and cultivars developed in many crop species. However, plant variety and cultivar identification via basic morphological features is problematic and challenging when differentiating closely related species not only due to their...
Article
Full-text available
A labelless DNA impedance biosensor for the detection of Erwinia mallotivora bacteria, the causal agent for papaya dieback disease, is developed based on commercial gold screen-printed electrode (SPE). Three types of Dropsens gold SPEs with different working electrode dimensions and gold ink curing temperature (i.e. 250BT, 250AT and 223AT) were com...
Article
Sensing founded on fluorescence quenching involving carbon dots (CDs) and gold nanoparticles (AuNPs) for the determination of Escherichia coli (E. coli) O157:H7 has been explored. CDs act as the fluorophore, while AuNPs as the quencher. Target oligos have been utilized to establish distance between CDs and AuNPs nanoparticles in close proximity. At...
Article
Full-text available
A fluorometric assay is described for highly sensitive quantification of Escherichia coli O157:H7. Reporter oligos were immobilized on graphene quantum dots (GQDs), and quencher oligos were immobilized on gold nanoparticles (AuNPs). Target DNA was co-hybridized with reporter oligos on the GQDs and quencher oligos on AuNPs. This triggers quenching o...
Article
Full-text available
Virus-like nanoparticles (VLNPs) have been studied extensively as nanocarriers for targeted drug delivery to cancer cells. However, VLNPs have intrinsic drawbacks, in particular, potential antigenicity and immunogenicity, which hamper their clinical applications. Thus, they can be eliminated easily and rapidly by host immune systems, rendering thes...
Poster
Full-text available
An innovative FRET system between GQDs and AuNPs has been demonstrated for the detection of E. coli O157:H7. Following the co-hybridization of target oligos, the fluorescence intensity of GQDs decreases slightly and the level of reduction of the GQDs with target oligos is greater than that of GQDs without target oligos. We improved several essentia...
Conference Paper
Full-text available
Agriculture losses due to crop infections are a major concern across the globe. In Malaysia, the persistent issue of papaya dieback disease is a threat to the industry. Advance disease detection in papaya is of paramount importance in order to minimize the damage, yield and economic losses. With this regard, early detection by electrochemical DNA b...
Conference Paper
Full-text available
A new DNA impedance biosensor for the detection of Erwinia mallotivora was successfully developed based on commercial gold screen-printed electrode (SPE) employing two unique genes selective towards the bacteria. Genome comparison via in silico and bioinformatic analysis on six different species of Erwinia has identified 45 gene clusters with 114 g...
Article
Full-text available
Diagnostic technologies for the detection of plant pathogens with point-of-care capability and high multiplexing ability are an essential tool in the fight to reduce the large agricultural production losses caused by plant diseases. The main desirable characteristics for such diagnostic assays are high specificity, sensitivity, reproducibility, qui...
Article
Full-text available
Developing quick and sensitive molecular diagnostics for plant pathogen detection is challenging. Herein, a nanoparticle based electrochemical biosensor was developed for rapid and sensitive detection of plant pathogen DNA on disposable screen-printed carbon electrodes. This 60 min assay relied on the rapid isothermal amplification of target pathog...
Article
Full-text available
Effective disease management strategies to prevent catastrophic crop losses require rapid, sensitive and multiplexed detection methods for timely decision making. To address this need, a rapid, highly specific and sensitive point-of-care method for multiplex detection of plant pathogens was developed by taking advantages of surface-enhanced Raman s...
Article
Full-text available
Developing molecular diagnostics in resource-poor settings is challenging. As such, we purpose-built a novel bridging flocculation assay for qualitative evaluation of isothermally amplified DNA by naked eye. The flocculation assay was dependent on pH, DNA polymer amounts and lengths. The method was first applied to the rapid and sensitive detection...
Article
Full-text available
Highly specific detection methods, capable of reliably identifying plant pathogens are crucial in plant disease management strategies to reduce losses in agriculture by preventing the spread of diseases. We describe a novel molecular inversion probe (MIP) assay that can be potentially developed into a robust multiplex platform to detect and identif...
Article
Full-text available
In many electrochemical DNA based biosensors, a redox indicator is required to be introduced separately to indicate the DNA hybridization event. In this work, we have developed a simple procedure for voltammetric determination of CaMV 35S gene modified DNA without the need for introduction of a redox indicator. The DNA biosensor contains an immobil...
Article
Full-text available
A regenerable electrochemical DNA biosensor based on a new type of acrylic microspheres and gold nanoparticles (AuNPs) composite coated onto a screen printed electrode (SPE) has been successfully developed for specific determination of the 35 S promoter from cauliflower mosaic virus (CaMV 35S) gene in soybean. DNA probe was immobilised onto acrylic...
Article
The SYBR Green I real-time PCR assay was used to quantify E. coli O157:H7 in various meat samples. Primers were designed to amplify and quantify the structural flagella (fliC) gene of E. coli O157:H7 in a single reaction. The primer specificity was confirmed with DNA from an ATCC culture of E. coli O157:H7 EDL933 as positive control, autoclaved E....
Article
Full-text available
The SYBR Green I real-time PCR assay was used to quantify E. coli O157:H7 in various meat samples. Primers were designed to amplify and quantify the structural flagella (fliC) gene of E. coli O157:H7 in a single reaction. The primer specificity was confirmed with DNA from an ATCC culture of E. coli O157:H7 EDL933 as positive control, autoclaved E....

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Projects

Projects (2)
Project
To develop DNA electrochemical biosensor for the determination of Musang King durian variety