Han-Jia Lin

National Taiwan Ocean University | ntou · Department of Bioscience and Biotechnology

Food safety concerns regarding foodborne pathogen contamination have gained global attention due to its significant implications. In this study, we developed a detection system utilizing a PCR array combined with an automated magnetic bead-based system and CE technology to enable the detection of three foodborne pathogens, namely Salmonella enteric...

This article presents a review of recent advancements in the utilization of NAA-based techniques for detecting foodborne pathogens in food products, focusing on studies conducted within the past five years. This review revealed that recent research efforts have primarily aimed at enhancing sensitivity and specificity by improving sample pre-treatme...

White spot syndrome virus (WSSV), an enveloped double-stranded DNA virus, is the causative agent of white spot syndrome (WSS), which has been linked to cultured shrimp mass mortality in many countries. Therefore, the development of anti-WSSV agents is among the top priorities of the aquaculture sector. Here, we describe the preparation of polyamine...

A highly inducible promoter capable of actively expressing exogenous genes in diatoms is critical to further improving the utility of this organism as a low-cost platform for industrial applications. In this study, the functional promoter of alkaline phosphatase gene in Phaeodactylum tricornutum (PtAPase) was used to develop a novel vector. The pPh...

Bacteremia and associated bacterial sepsis are potentially fatal and occur when the host response to microbial invasion is impaired or compromised. This motivated us to develop carbonized polymer dots (CPDsMan/AA) from a mixture of mannose (Man) and positively charged amino acids [AAs; lysine, arginine (Arg), or histidine] through a one-step mild p...

Japanese encephalitis is a mosquito-borne disease caused by the Japanese encephalitis virus (JEV) that is prevalent in Asia and the Western Pacific. Currently, there is no effective treatment for Japanese encephalitis. Curcumin is a compound extracted from the roots of Curcuma longa, and many studies have reported its antiviral and anti-inflammator...

Inflenza A viruses (IAVs) are highly transmissible and pathogenic Orthomyxoviruses, which have led to worldwide outbreaks and seasonal pandemics of acute respiratory diseases, causing serious threats to public health. Currently used anti-influenza drugs may cause neurological side effects, and they are increasingly less effective against mutant str...

Infectious diseases are considered the greatest threat to the modern high-density shrimp aquaculture industry. Specificity, rapidity, and sensitivity of molecular diagnostic methods for the detection of asymptomatic infected shrimp allows preventive measures to be taken before disease outbreaks. Routine molecular detection of pathogens in infected...

Carbon-based nanomaterials have great potential in medical applications, especially in the treatment of infectious diseases and even tumors. However, to safely execute the application of carbon nanomaterials in human treatments, conducting safety assessments and establishing suitable evaluation criteria are necessary. In this study, lysine-carboniz...

Most dry eye syndromes (DES) are caused by oxidative stress and an overactive inflammatory response, leading to tear deficiency and excessive tear evaporation. Conventional eye drops for DES treatment require high doses and frequent administration due to their insufficient precorneal residence time. To overcome these problems, in this study, we hav...

Background Shrimp aquaculture has suffered huge economic losses over the past decade due to the outbreak of acute hepatopancreatic necrosis disease (AHPND), which is mainly caused by the bacteria Vibrio parahaemolyticus ( V. parahaemolyticus ) with the virulence pVA1 plasmid, which encodes a secretory photorhabdus insect-related (Pir) toxin compose...

Developing antimicrobial agents that can eradicate drug-resistant (DR) bacteria and provide sustained protection from DR bacteria is a major challenge. Herein, we report a mild pyrolysis approach to prepare carbon nanogels (CNGs) through polymerization and the partial carbonization of l-lysine hydrochloride at 270 °C as a potential broad-spectrum a...

Background: Shrimp aquaculture has suffered huge economic losses over the past decade due to the outbreak of acute hepatopancreatic necrosis disease (AHPND), which is mainly caused by the bacteria Vibrio parahaemolyticus (V. parahaemolyticus) with the virulence pVA1 plasmid, which encodes a secretory photorhabdus insect-related (Pir) toxin composed...

Marine oomycetous species produce, among other fatty acids, omega-6 arachidonic acid (ARA) and omega-3 eicosapentaenoic acid (EPA), with implications for the industrial potential of this group of organisms and the need to find an isolate with high production. This study screened 14 isolates of marine oomycetous species: Halophytophthora avicenniae...

The study of the sinking phenomenon of diatom cells, which have a slightly larger specific gravity (~1.3) compared to that of water, is an important research topic for understanding photosynthetic efficiency. In this study, we successfully demonstrated the observation of the sinking behaviors of four different species of diatom using a homemade “tu...

We have demonstrated that alginate with negligible anticoagulant activity can be converted into carbonized nanogels with potent anticoagulant activity through a solid-state heating process. The conversion of alginate into graphene-like nanosheet (GNS)-embedded polyphenolic-alginate nanogels (GNS/Alg-NGs) has been carried out through condensation an...

In this study, we demonstrate the synthesis of carbonized nanogels (CNGs) from an amino acid (lysine hydrochloride) using a simple pyrolysis method, resulting in effective viral inhibition properties against infectious bronchitis virus (IBV). The viral inhibition of CNGs was studied using both in vitro (bovine ephemeral fever virus (BEFV) and pseud...

Carbon quantum dots (CQDs) are emerging novel nanomaterials with a wide range of applications and high biocompatibility. However, there is a lack of in-depth research on whether CQDs can cause acute or long-term adverse reactions in aquatic organisms. In this study, two different types of CQDs prepared by ammonia citrate and spermidine, namely CQDA...

To prevent visual loss, clinical therapy of severe bacterial keratitis (BK) demands simultaneous remedying of the damage caused by bacterial infection and its induced oxidative stress/inflammation of the cornea. Here, we have developed a one-step method to synthesize carbonized nanogels (CNGs) from biogenic quercetin (Qu) and lysine (Lys) as a bifu...

White spot syndrome virus (WSSV) is the causative agent of white spot syndrome (WSS), a disease that has led to severe mortality rates in cultured shrimp all over the world. The WSSV is a large, ellipsoid, enveloped double-stranded DNA virus with a wide host range among crustaceans. Currently, the main antiviral method is to block the receptor of t...

Graphene quantum dots (GQDs) are emerging disinfectants, owing to their distinctive photodynamic and antibacterial activities. Halogen-based antimicrobial materials are also known to be effective against infectious pathogens. In this work, we have developed visible light-activatable halogen/nitrogen co-doped polymeric graphene quantum dots [X/N–PGQ...

The world’s first natural avian-origin H6N1 influenza A virus infection case in dogs was confirmed in Taiwan in 2014. The H6N1 virus in chickens has been endemic in Taiwan since 1972. Whether the dog H6N1 virus has interspecies transmission potential is the key issue we aim to understand. Following one virus passage in embryonated eggs and two furt...

Bacterial infectious diseases cause a huge economic loss in aquaculture. Active biological control that uses bacteriovorous organisms to remove pathogens is an ecologically friendly approach for the cultural system to counteract the bacterial infection. The ciliate is one of the main predators of bacteria in aquatic ecosystems, but whether it can b...

The pandemic of avian influenza viruses (AIVs) in Asia has caused enormous economic loss in poultry industry and human health threat, especially clade 2.3.4.4 H5 and H7 subtypes in recent years. The endemic chicken H6 virus in Taiwan has also brought about human and dog infections. Since wild waterfowls is the major AIV reservoir, it is important t...

The combination of different nanomaterials has been investigated during the past few decades and represents an exciting challenge for the unexpected emerging properties of the resulting nano-hybrids. Spermidine (Spd), a biogenic polyamine, has emerged as a useful functional monomer for the development of carbon quantum dots (CQDs). Herein, an elect...

We demonstrate the synthesis of antibacterial carbon quantum dots (CQDs) through a one-step pyrolysis of biogenic polyamine (PA) and dopamine (DA) mixture. SPM/DA-CQDs synthesized from DA combined with spermine (SPM) exhibit effective antibacterial activity and high adhesion properties on glass and surfaces of polymeric contact lens material. The a...

In article number 1902641, Robert Y. L. Wang, Shiow‐Yi Chen, Chih‐Ching Huang, and co‐workers develop a simple one‐step dry heating process which allows the synthesis of core–shell carbon quantum dots derived from curcumin. The obtained carbon quantum dots possess excellent antiviral activity against enterovirus 71 through a complex inhibitory mech...

It is demonstrated that carbon quantum dots derived from curcumin (Cur‐CQDs) through one‐step dry heating are effective antiviral agents against enterovirus 71 (EV71). The surface properties of Cur‐CQDs, as well as their antiviral activity, are highly dependent on the heating temperature during synthesis. The one‐step heating of curcumin at 180 °C...

We have developed a one-pot synthesis of bio-carbon nanowires from the natural product sodium alginate at low temperature, without using any catalyst, for anticoagulation applications. Sodium alginate is carbonized and sulfated/sulfonated in situ by solid state heating of a mixture of sodium alginate and ammonium sulfite. By regulating the heating...

Microalgae of different evolutionary origins are typically found in rivers, lakes, and oceans, providing more than 45% of global primary production. They provide not only a food source for animals, but also affect microbial ecosystems through symbioses with microorganisms or secretion of some metabolites. Derived from amino acids, polyamines are pr...

Background: Transcriptomic sequencing (RNA-seq) related applications allow for rapid explorations due to their high-throughput and relatively fast experimental capabilities, providing unprecedented progress in gene functional annotation, gene regulation analysis, and environmental factor verification. However, with increasing amounts of sequenced...

In this paper, we report a simple one-step synthesis method for silver-gold bimetallic nanoparticles deposition on silver chloride nanosheets to form Ag-Au/AgCl nanohybrid with oxidase-like and peroxidase-like catalytic activity. We used these nanohybrid in the detection of spermine. First, 13 nm-sized Au NPs were synthesized by citrate reduction o...

Increasing water consumption and diminishing fresh water resources have created the need for new water treatment technologies to supply safe water for domestic and industrial needs. The development of polymeric nanofiltration (NF) membrane technology led to water treatment at lower operating pressures than that of reverse osmosis. NF membranes reje...

Gene detection has an important role in diagnosing several serious diseases and genetic defects in modern clinical medicine. Herein, we report a fast and convenient gene detection method based on the modulation of the interaction between a heat-resistant double-stranded DNA (dsDNA)-binding protein (Sso7d) and gold nanoparticles (Au NPs). We prepare...

Using polyamines as the initial organic raw material and by applying simple pyrolysis methods, super cationic carbon quantum dots (CQDs) can easily be made. Since polyamines are natural products and the synthesis procedure is green, these polyamine-derived CQDs display low toxicity and high biocompatibility but possess high antibacterial activity....

We have developed a one-step method to synthesize carbon quantum dots (CQDPAs) from biogenic polyamines (PAs) as an antibacterial agent for topical treatment of bacterial keratitis (BK). CQDs synthesized by direct pyrolysis of spermidine (Spd) powder through a simple dry heating treatment exhibit a solubility and yield much higher than those from p...

Angiogenesis is the process of formation of new blood vessels, which is essential to human biology, and also plays a crucial role in several pathologies such as tumor growth and metastasis, exudative age-related macular degeneration, and ischemia. Vascular endothelial growth factor (VEGF), in particular, VEGF-A165 is the most important pro-angiogen...

This study reports a two-step method to synthesize spermidine-capped fluorescent carbon quantum dots (Spd–CQDs) and their potential application as an antibacterial agent. Fluorescent carbon quantum dots (CQDs) are synthesized by pyrolysis of ammonium citrate in the solid state and then modified with spermidine by a simple heating treatment without...

Subtle morphogenesis begins during trunk development after the segmentation period in zebrafish. Sprouting angiogenesis forms the trunk vascular system after the extension of the dorsal aorta and cardinal veins. The posterior lateral line primordium migrates from head to tail, forming the sensory organs on the skin. It is still unclear whether this...

We demonstrated that thrombin-binding aptamer-conjugated gold nanoparticles (TBA-Au NPs), prepared from a self-assembled hybrid monolayer (SAHM) of triblock aptamers on Au NPs (13nm), can effectively inhibit thrombin activity toward fibrinogen. The first block poly(adenine) at the end of the triblock TBA was used for the self-assembly on Au NP surf...

In this paper, we describe the use of pulsed laser desorption/ionization mass spectrometry (LDI-MS) for the detection of tumor cells through the analysis of gold cluster ions [Aun](+) from aptamer-modified gold nanofilms (Au NFs). We observed not only the transformation of the Au NFs into gold nanoparticles (Au NPs) but also the formation of gaseou...

Polyunsaturated fatty acids (PUFAs) are fatty acids with more than one double bond in the chemical structure. Arachidonic acid (ARA, 20:4 (n-6)) and eicosapentaenoic acid (EPA, 22:5 (n-3)) are common PUFAs with beneficial health effects. Marine fish and meat are the main sources of omega-3 and omega-6 fatty acids in human's diet, respectively. In p...

The liver plays a vital role in metabolism, detoxification, digestion, and the maintenance of homeostasis. During development, the vertebrate embryonic liver undergoes a series of morphogenic processes known as hepatogenesis. Hepatogenesis can be separated into three interrelated processes: endoderm specification, hepatoblast differentiation, and h...

Adaptation of enzymes in a metabolic pathway can occur not only through changes in amino acid sequences but also through variations in transcriptional activation, mRNA splicing and mRNA translation. The heme biosynthesis pathway, a linear pathway comprised of eight consecutive enzymes in animals, provides researchers with ample information for mult...

We have developed aptamer (Apt)-conjugated gold nanoparticles (Apt-Au NPs, 13 nm in diameter) as highly effective inhibitors for human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT). Two Apts, RT1t49 (Apt(pol)) and ODN 93 (Apt(RH)), which recognize the polymerase and RNase H regions of HIV-1 RT, are used to conjugate Au NPs to prepa...

In phosphorus-deficient conditions, Phaeodactylum tricornutum releases an alkaline phosphatase (PtAPase) to the medium that is readily detectable by activity staining. Nucleic acid and amino acid sequence of this alkaline phosphatase (APase) was identified by performing proteomic analysis and database searches. Sequence alignment suggests that PtAP...

Spermidine/spermine N(1)-acetyltransferase 1 (Ssat1) is a key enzyme in the polyamine interconversion pathway, which maintains polyamine homeostasis. In addition, mammalian Ssat1 is also involved in many physiological and pathological events such as hypoxia, cell migration, and carcinogenesis. Using cross-genomic bioinformatic analysis in 10 deuter...

Oligonucleotide primers used in this work. (DOC)

Alignment of zebrafish ssat1 homologues cDNA sequence. The cDNA sequences were aligned by MegAlign (Lasergene) with the ClustalW method. The conserved residues are shaded black. The denoted amino acid sequences underneath cDNA sequences are consensus residues in all three homologues, while the encoded amino acids which are not conserved in all thre...

Chromosomal localizations of human SSAT1 and zebrafish ssat1 homologues. (TIF)

Translational regulation of ssat1 genes in zebrafish cells. ZF4 cells were transiently transfected with the plasmid encoding myc-tagged full-length human SSAT1, zebrafish Ssat1a, or Ssat1b. After incubation for 12 h, transfected cells were treated with 10 µM DENSPM, 20 µM MG132, or vehicle for 24 h. Cell lysates (5 µg total protein in each lane) we...

Sequence alignment of integrin α9. The amino acid sequences of human (NP_002198), mouse (NP_598482), and zebrafish integrin α9 (XP_003199805) were aligned by MegAlign (Lasergene) with the ClustalW method. The conserved residues are shaded in black. The cytosolic domains are marked with a red box. (TIF)

Sequence alignment of human, mouse and zebrafish Hif-1α. The amino acid sequences of human (NP_001521), mouse (NP_034561), and zebrafish Hif-1α (AAQ91619) were aligned by MegAlign (Lasergene) with the ClustalW method. The conserved residues are shaded in black. The PAS-B domain is underlined in red. (TIF)

In this study we used photoluminescent 11-mercaptoundecanoic acid-bound gold nanodots (11-MUA-Au NDs) to detect hemoglobin through photoluminescence (PL) quenching. The mechanism of quenching, which occurred through redox reactions between the 11-MUA-Au NDs and the Fe(II) atoms of hemin units, was supported by an increase in the signals (G 2.0 and...

Specificity of antiserum against SVS I, SVS II, or SVS III. (a) The potential epitopes were predicted along the amino acid sequences of SVS I–III. A polypeptide fragment with relatively high antigenic index appeared in residues 281–428 of SVS I, residues 260–375 of SVS II, or residues 77–109 of SVS III. Antiserum against each of the 3 recombinant p...

Ankyrin repeat domain (ARD) proteins contain various numbers of internal repeat units. They are considered as one important factor to influence hypoxia response through hydroxylation interaction with Factor Inhibiting HIF (FIH) enzymes which can repress HIF under normoxia environment. In this study, we adopted sequence based method and applied cons...

Formation of copulatory plugs by male animals is a common means of reducing competition with rival males. In mice, copulatory plugs are formed by the coagulation of seminal vesicle secretion (SVS), which is a very viscous and self-clotting fluid containing high concentration of proteins. In its native state, mouse SVS contains a variety of disulfid...

Spermidine/spermine N(1)-acetyltransferase 1 (SSAT1) is a key enzyme that catalyzes the catabolism of polyamines. SSAT1 is a very important enzyme because it not only maintains the homeostasis of polyamines but also is involved in many physiological and pathological events. As such, a rapid assay of SSAT1 activity is valuable in drug screening and...

The existence of surfactants in proteomics samples can severely reduce enzymatic digestion efficiency, liquid chromatography (LC) separation efficiency, column lifetime, and mass spectrometry (MS) sensitivity. Although various techniques are able to remove surfactants, surfactants may occasionally be retained in samples due to variations in sample...

Two-dimensional electrophoresis (2-DE) has evolved into a robust separation technique in proteomic research. However, one of the major challenges in 2-DE experiments, the reproducibility of the first dimensional electrophoresis (IEF), has remained unsolved. It is well-known that the quality of IEF experiments is significantly affected by the salt i...

We have analyzed peptides, proteins, and protein-drug complexes through surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) using HgTe nanostructures as matrixes. We investigated the effects of several parameters, including the concentration of the HgTe nanostructures, the pH of the buffer, and the concentration of salt, on th...

We identified a testis-specific protease-like protein tentatively named TESPL and a pancreatic trypsinogen Prss2 from the clones of a yeast two-hybrid screen against a mouse testicular cDNA library using the trypsin inhibitor Spink3 from male accessory sexual glands as bait. The enzymatic motifs and the cysteine patterns in serine proteases are hig...

SVS I was exclusively expressed in seminal vesicle in which the protein was immunolocalized primarily to the luminal epithelium of mucosal folds. The developmental profile of its mRNA expression was shown to be androgen-dependent, manifesting a positive correlation with the animal's maturation. There are 43 glutamine and 43 lysine residues in one m...

A 75-kDa protein secreted from mouse coagulating gland was purified to homogeneity by a series of isolation steps including ion exchange chromatography on a DEAE-Sephacel column and ion exchange high-performance liquid chromatography on a sulfopropyl column. It was identified to be Type IV transglutaminase (TG(4)), based on the establishment of N-t...

No monomer forms of SVS I-III are found in mouse seminal vesicle secretion (SVS), despite detection of these within the SVS by reduced SDS-PAGE. Instead, they ware found to have been selectively cross-linked by inter-polypeptide disulfide bridges to give various high molecular weight complexes (HMWCs). Although SVSI-III can be immunochemically stai...

P12 is a Kazal-type trypsin inhibitor that has been purified from mouse seminal vesicle secretion. We observed a slight impact of P12 on sperm capacitation, and demonstrated the removal of plasma membrane overlaying the acrosome region by immunoaggregation of P12 on mouse sperm. Further, we compared the immunoreactivity of P12 antibody to ten P12 v...

Among the major proteins in the rat seminal vesicle secretion, transglutaminase catalyzed the cross-links among RSVS I-III. Six peptide sequences determined from the trypsin digests of RSVS III were confirmed in the protein sequence derived from two paralogs, RSVS III(alpha) and RSVS III(beta) gene, in rat 3q42. Their transcription units are organi...