Han Cheng

• PhD
• Deputy Director at Rubebr Research Insitute . Chinese Academy of Tropical Agricultural Sciences (CATAS)

In the present study, we compared the transcriptome profiles of H2O2 treated dark grown Arabidopsis with those grown under the normal light. In total, 2050 and 8540 genes showed altered expression under H2O2 and light treatment respectively, in which 1595 were co-regulated by both H2O2 and light. Among the 1595 genes, 1027 were up-regulated and 486...

The rubber tree (Hevea brasiliensis) is a tropical, perennial, woody plant that is susceptible to cold stress. In China, cold stress has been found to severely damage rubber plants in plantations in past decades. Although several Hevea clones that are resistant to cold have been developed, their cold hardiness mechanism has yet to be elucidated. Fo...

With recent advances of next-generation sequencing technology, RNA-Sequencing (RNA-Seq) has emerged as a powerful approach for the transcriptomic profiling. RNA-Seq has been used in almost every field of biological studies, and has greatly extended our view of transcriptomic complexity in different species. In particular, for nonmodel organisms whi...

Rubber trees are economically important tropical tree species and the major source of natural rubber, which is an essential industrial material. This tropical perennial tree is susceptible to cold stress and other abiotic stresses, especially in the marginal northern tropics. Recent years, the genome sequencing and RNA-seq projects produced huge am...

Rubber trees (Hevea brasiliensis) are susceptible to low temperature and therefore are only planted in the tropical regions. In the past few decades, although rubber trees have been successfully planted in the northern margin of tropical area in China, they suffered from cold injury during the winter. To understand the physiological response under...

Citation: Bian, X.; Wu, T.; Qiang, R.; Deng, Z.; Rehman, F.; Han, Q.; Xu, D.; Yuan, Y.; Wang, X.; An, Z.; et al. Genome-Wide Identification and Expression Profile of Farnesyl Pyrophosphate Synthase (FPS) Gene Family in Euphorbia Hirta L. Int. J. Mol. Sci. 2025, 26, 798. https://doi. Abstract: The biosynthesis of isopentenyl diphosphate (IPP) and di...

The biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), which are essential for sesquiterpenes and triterpenes, respectively, is primarily governed by the mevalonate pathway, wherein farnesyl pyrophosphate synthase (FPS) plays a pivotal role. This study identified eight members of the FPS gene family in Euphorbia hi...

The basic helix-loop-helix (bHLH) transcription factors play crucial roles in plant growth, development, and stress responses. However, their identification and insights into the understanding of their role in rubber trees remain largely uncovered. In this study, the bHLH gene family was explored and characterized in rubber trees using systematic b...

This study delves into the pivotal role of sulfur vulcanization in defining the mechanical characteristics of natural rubber (NR) latex-dipped products. Utilizing sulfur vulcanization, known for its operational simplicity and cost-effectiveness, we examine its ability to enhance product elasticity and mechanical strength through various sulfidic bo...

Abstract: GRAS is a plant-specific transcription factor, which plays an important regulatory role in plant growth and development, and abiotic stress response. GRAS gene family has not been reported in Hevea brasiliensis. In this study, bioinformatics tools were used to analyze the physicochemical properties, phylogenetic relationships, gene struct...

The basic helix loop helix (bHLH) transcription factors play crucial roles in plant growth, development, and stress responses. However, their identification and insight understanding in rubber trees remain largely uncovered. In this study, the bHLH gene family was explored and characterized in rubber trees using systematic bioinformatics approaches...

Rubber tree (Hevea brasiliensis), as the primary source of natural rubber (NR), holds a significant economic significance. The high-quality genome has been long pursued to understand the rubber production process, genomic structure, and genomics-assisted breeding. Here, we assemble the first haplotype-resolved telomere-to-telomere (T2T), gap-free r...

Natural rubber isoneofthemostimportant strategic resources, and98%ofitisderived fromrubber trees. InChina, theplanting area forrubber treesislimited tothenorthern edgeofthetropical region, which isoften affected bystrong wind andlow-temperature conditions. Intheearly stages, rubber industry experiences several setbacks. Therefore, thebreeding ofstr...

The rubber tree (Hevea brasiliensis) is grown in tropical regions and is the major source of natural rubber. Using traditional breeding approaches, the latex yield has increased by six‐fold in last century. However, the underlying genetic basis of rubber yield improvement is largely unknown. Here we present a high‐quality, chromosome‐level genome s...

Ultrahigh-density genetic mapping is used extensively for gene discovery, mapping quantitative trait loci (QTL), and assisting in crop breeding. However, genetic mapping of rubber trees (Hevea brasiliensis) has hitherto been limited in terms of both types and resolution. In this study, 214 F1 progenies derived from a cross of IAN 873 × REYAN 106 we...

Small nucleolar RNAs (snoRNAs) are a class of conserved nuclear RNAs that play important roles in the modification of ribosomal RNAs (rRNAs) in plants. In rubber trees, rRNAs are run off with latex flow during tapping and need to be regenerated for maintaining the functions of the laticifer cells. SnoRNAs are expected to play essential roles in the...

Abiotic stress severely affects the natural rubber yield in China, so it is necessary to clarify the resistant mechanism of rubber tree to improve natural rubber yield. In previous studies, we found that a MAPPKKK was induced by low temperature in rubber tree. Sequence alignment showed that HbMAPKKK is homologous with MAPKKK15 from Arabidopsis. How...

Hevea brasiliensis is an important tropical crop, and its economical value comes from the nature rubber produced in the laticifers in the bark. The proteins in the bark and laticifers are the majortargets in the study of the rubber biosynthesis and regulation. However, the total protein extraction in therubber tree bark is difficult due to the abun...

A GGGCC motif (site II like motif) was identified from the upstream sequences of reactive oxygen species (ROS) and light induced genes in Arabidopsis. This motif is highly enriched within the -50 to -250 bp region of the induced genes, and it is also specifically distributed in the same region in mouse and human genome. EMSA experiments revealed th...

With rapid research advances in the genome of the Hevea brasiliensis rubber tree, more genomic and transcriptomic data have been generated. However, these data are still deposited in raw format in the National Centre for Biotechnology Information (NCBI) and other public databases, and therefore, data utilization is cumbersome for the scientists in...

The Hevea brasiliensis rubber tree is an important industrial crop that is widely planted in the tropical regions. China has a rubber planting history of more than 100 years and has successfully established three planting districts: Yunnan, Guangdong, and Hainan. More than 100 clones raised by Chinese breeders are widely cultivated. However, it is...

With the proceedings in the genome study in rubber tree, more and more data were produced. However, these data are still deposited in public databases as raw format, and the utilization of these NGS data is hindered for the researchers in rubber tree community. HeveaDB was therefore constructed and maintained under the organization of International...

Background Forward genetics approaches are not popularly applied in non-model plants due to their complex genomes, long life cycles, backward genetic studies etc. Researchers have to adopt reverse genetic methods to characterize gene functions in non-model plants individually, the efficiency of which is usually low. ResultsIn this study, we report...

The Para rubber tree (Hevea brasiliensis) is an economically important tropical tree species that produces natural rubber, an essential industrial raw material. Here we present a high-quality genome assembly of this species (1.37 Gb, scaffold N50 = 1.28 Mb) that covers 93.8% of the genome (1.47 Gb) and harbours 43,792 predicted protein-coding genes...

An EBP1 gene was identified from rubber tree in this study，which full length cDNA sequence of HbEBP1 gene is 1462-bp in length, with a 141-bp 5’ UTR and a 133-bp 3’ UTR.An intact ORF was found in HbEBP1 cDNA sequence, which encodes 395 aa poly-peptide. The deduced HbEBP1 protein has a molecular weight of 43.596 kD, PI 6.45. Bioinformatics analysis...

CRT/DRE Binding Factor 1 (CBF1) overexpression enhanced freezing tolerance in Nicotiana tabacum. To unravel the proteomics changes caused by the ectopic overexpression of AtCBF1 genes, we utilized 2-DE along with MALDI-TOF-MS and LC ESI-MS/MS to study the proteome profiles. We also tried to identify the proteins that significantly change in abundan...

Redox regulation and signaling, which are involved in various cellular processes, have become one of the research focuses in the past decade. Cysteine thiol groups are particularly susceptible to post-translational modification, and their reversible oxidation is of critical role in redox regulation and signaling. With the tremendous improvement of...

Normal 0 7.8 pt 0 2 false false false EN-US ZH-CN X-NONE MicrosoftInternetExplorer4 We have developed a rapid extraction method using low salt concentration buffer for the isolation of low-molecular-weight RNA from Arabidopsis tissues. The method was quick and efficient, and the small scale extraction process took no more than 1 hour, while yield a...

Silver staining of nucleic acid has been widely used in molecular marker analysis such as simple sequence repeat (SSR), single-strand conformation polymorphism (SSCP), and amplified fragment-length polymorphism (AFLP). Many alternatives to silver staining methods have been described, but these methods are not efficient or cost-effective. Here we re...

Three thousand and ninety Unigenes were obtained from 10 778 Hevea brasiliensis ESTs. Four hundred and thirty SSRs were distributed in 353 Unigenes, which accounts for 11.42% of the total number of Unigenes. The frequency of SSRs was 1/3.93 kb. Dinucleotide and trinucleotide repeats were the dominant types among the obtained unigenes, accounting fo...

CBF pathway is the most important pathway during the process of cold acclimation in plants. In previous study, we cloned a CBF like gene HbCBF1 from Hevea brasiliensis, which implied that CBF pathway might exist in this kind of tropical oriented tree. However, it was still unknown if this gene functioned as C-repeat binding factor. Therefore it was...

Rubber trees of Clone 93-114 were treated under cold stress,and total RNA and mRNA were extracted from young branches and leaves of the rubber tree.cDNA was then synthesized by SMART technique,and digested by Sfi I.The prepared cDNA was ligated into the vector of pDNR-LIB,and transformed into E.Coli by electroporation.The constructed full-length cD...