Hala Zreiqat

Hala Zreiqat
The University of Sydney · school of Biomedical Engineering

About

222
Publications
31,920
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9,269
Citations
Citations since 2017
67 Research Items
4692 Citations
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20172018201920202021202220230200400600800
20172018201920202021202220230200400600800

Publications

Publications (222)
Preprint
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In recent years, the development of carbon dot-based fluorescent nanoparticles for bioimaging applications has attracted the attention of scientific community. However, the functionality of the majority of these developed fluorescent systems is confined in to the blue-to-green region of the light spectrum, limiting their application as bioimaging a...
Preprint
In the final step of cancer metastasis, tumor cells become lodged in a distant capillary bed, where they can undergo extravasation and form a secondary tumor. While increasing evidence suggests blood/lymphatic flow and shear stress play a critical role in the tumor extravasation process, there is a lack of systematic and biomechanical approaches to...
Article
Combating the accumulated senescent cells and the healing of osteoporotic bone fracture in the elderly remains a significant challenge. Nicotinamide mononucleotide (NMN), a precursor of NAD +, is an excellent candidate for mitigating aging-related disorders. However, it is unknown if NMN can alleviate senescent cell induction and enhance osteoporot...
Article
Full-text available
Forming ceramics into rationally-designed and complex shapes without compromising their mechanical properties is a major challenge. Here, we demonstrate self-shaping of ceramics through sequential stereolithographic printing of ceramic resins into components with a heterogeneous concentration of ceramic particles, resulting in well-defined anisotro...
Article
The tissue engineering approach for repair and regeneration has achieved significant progress over the past decades. However, challenges remain in developing strategies to solve the declined or impaired innate cell and tissue regeneration capacity that occurs with aging. Cellular senescence is a key mechanism underlying organismal aging and is resp...
Article
3D printed bone scaffolds have the potential to replace autografts and allografts because of advantages such as unlimited supply and the ability to tailor the scaffolds’ biochemical, biological and biophysical properties. Significant progress has been made over the past decade in additive manufacturing techniques to 3D print bone grafts, but challe...
Preprint
Terahertz (THz) technology is a growing and multi-disciplinary research field, particularly for sensing and telecommunications. A number of THz waveguides have emerged over the past years, which are set to complement the capabilities of existing and bulky free space setups. In most designs however, the guiding region is physically separated from th...
Article
A strontium-doped hardystonite (Sr-HT) bioceramic, in bulk form, demonstrates excellent bioactivity for bone regeneration with high fracture toughness and compressive strength. This work examines the antibacterial and mechanical properties of Sr-HT coatings on an alpha-beta titanium alloy with a high specific strength and excellent corrosion resist...
Article
Full-text available
Reproducible fabrication of concave cubic gold nanoparticles with precise control over size, concaveness, and aspect ratio is important because the nanoscale structural characteristics can influence their plasmonic and catalytic properties. However, this is particularly challenging because the number of synthetic parameters involved in the fabricat...
Article
There is an unmet clinical need for a spinal fusion implant material that recapitulates the biological and mechanical performance of natural bone. We have developed a bioceramic, Sr-HT-Gahnite, which has been identified as a potential fusion device material. This material has the capacity to transform the future of the global interbody devices mark...
Chapter
Plasmonics deals with the interaction of the electromagnetic field of an emitter with the noble metal nanoparticles or nanosheets through the evanescent wave coupling. The emitter can either be excited directly by an excitation light or by the evanescent field itself. Excitation through the evanescent field works as a sensor as these fields are sen...
Article
Internal architecture of tissue scaffolds plays a significant role in their ability to heal critical-size bone defects. Many studies have investigated these effects but lack isolating architectural features in 3D space, hindering optimization of pore shape to improve bone ingrowth and consequently clinical outcome. To address this challenge, we dev...
Article
Bone's outstanding biomechanical performance is derived from cooperative interactions between its composition and microarchitecture. Towards developing bioceramic scaffolds with similar biomechanical performance for repairing large bone defects under load, we have developed 13 new bioceramic compositions by doping various concentrations of iron and...
Article
Full-text available
Carbon dots have been gaining attention in the field of nanobiotechnology due to their superior photostability, high water solubility, ease of synthesis and surface functionalization, chemical inertness, low toxicity, and excellent biocompatibility. They also exhibit good two-photon absorption and unique tunable optical properties across a wide ran...
Article
Designing a two-photon ratiometric pH-sensitive nanoprobe for real time monitoring of intracellular pH in biological environment is of great importance for better understanding the pathogenesis of diseases and the design of intracellular drug delivery-based system. However, the development of such probe remains a challenge. Here, we report for the...
Article
Carbon based quantum dots find various applications such as imaging, diagnosis and therapeutics due to low toxicity, antibacterial activity, excellent water solubility, and good biocompatibility. However, the UV-blue emission of carbon based quantum dots is unsuited for applications in biomedicine and imaging as the short wavelength lights harm the...
Article
Understanding the surface processes (deposition and surface diffusion) that occur at or close to the surface of growing nanoparticles is important for fabricating reproducibly stellated or branched gold nanoparticles with precise control over arm length and spatial orientation of arms around the core. By employing a simple seed-mediated strategy, w...
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Computational modeling methods combined with non-invasive imaging technologies have exhibited great potential and unique opportunities to model new bone formation in scaffold tissue engineering, offering an effective alternate and viable complement to laborious and time-consuming in vivo studies. However, existing numerical approaches are still hig...
Article
Recently, carbon dots (CDs) have been widely investigated for biological applications in imaging. One-step hydrothermal synthesis is considered to be one of the most promising methods for the synthesis of CDs, due to its simple and rapid manipulation, flexible selection of ingredients, environmentally friendly conditions, and low-cost. A number of...
Article
Full-text available
Among various magnetic nanoparticles, manganese oxide nanoparticles are considered as established T 1 magnetic resonance imaging (MRI) contrast agents for preclinical research. The implications of their degradation properties and use as therapeutic carriers in drug delivery systems have not been explored. In addition, how the chemical composition a...
Preprint
Full-text available
Understanding the surface processes (deposition and surface diffusion) that occur at or close to the surface of growing nanoparticles is important for fabricating reproducibly stellated or branched gold nanoparticles with precise control over arm length and spatial orientation of arms around the core. By employing a simple seed-mediated strategy, w...
Article
Full-text available
An ongoing challenge in the field of orthopedics is to produce a clinically relevant synthetic ceramic scaffold for the treatment of ‘critical-sized’ bone defects, which cannot heal without intervention. We had developed a bioactive ceramic (Baghdadite, Ca₃ZrSi₂O₉) and demonstrated its outstanding bioactivity using traditional manufacturing techniq...
Article
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In this study, the hydraulic reactivity and cement formation of baghdadite (Ca3ZrSi2O9) was investigated. The material was synthesized by sintering of a mixture of CaCO3, SiO2 and ZrO2 and then mechanically activated using a planetary mill. This leads to a decrease in particle and crystallite size and a partial amorphization of baghdadite as shown...
Preprint
Pluripotent-stem-cell-derived tissue-models have been established with increasingly physiological shape, size and function. However, the histogenic and morphogenetic processes present in these models proceed stochastically. This reflects an absence of technologies able to produce complex supportive cell niches that can reproducibly guide tissue pat...
Article
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This work studies the mechanical and biological properties of Baghdadite (BAG, Ca3ZrSi2O9) coating manufactured on Ti6Al4V substrates by hybrid water-stabilized plasma spray (WSP-H). Hydroxyapatite (HAp, Ca10(PO4)6(OH)2) coating was produced by gas-stabilized atmospheric plasma spray and used as a reference material. Upon spraying, the BAG coating...
Article
Bone fractures and critical-sized bone defects present significant health threats for the elderly who have limited capacity for regeneration due to the presence of functionally compromised senescent cells. A wide range of synthetic materials has been developed to promote the regeneration of critical-sized bone defects, but it is largely unknown if...
Article
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Surface topography is one of the key factors in regulating interactions between materials and cells. While topographies presented to cells in vivo are non-symmetrical and in complex shapes, current fabrication techniques are limited to replicate these complex geometries. In this study, we developed a microcasting technique and successfully produced...
Article
Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal solid malignancies in the world due to its insensitivity to current therapies and its propensity to metastases from the primary tumor mass. This is largely attributed to its complex microenvironment composed of unique stromal cell populations and extracellular matrix (ECM). The recru...
Article
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Engineering synthetic scaffolds to repair and regenerate ruptured native tendon and ligament (T/L) tissues is a significant engineering challenge due to the need to satisfy both the unique biological and biomechanical properties of these tissues. Long‐term clinical outcomes of synthetic scaffolds relying solely on high uniaxial tensile strength are...
Article
Carbon dots (CDs) based nanoparticles have been extensively explored for biological applications in sensing and bioimaging. However, the major translational barriers to CDs for imaging and sensing applications include synthetic strategies to obtain monodisperse CDs with tunable structural, electronic and optical properties in order to achieve high-...
Article
The development of suitable synthetic scaffolds for use as human tendon grafts to repair tendon ruptures remains a significant engineering challenge. Previous synthetic tendon grafts have demonstrated suboptimal tissue ingrowth and synovitis due to wear particles from fiber-to-fiber abrasion. In this study, we present a novel fiber-reinforced hydro...
Article
Biological and mechanical functions are sometimes two conflicting characteristics in bone tissue scaffolds, which necessitates a trade-off between these two properties in load-bearing applications. In this article, a systematic computational analysis was performed to investigate the effects of controllable fabrication factors (e.g. Design for Addit...
Article
Full-text available
Materials with dense architectures are composed of stiff and strong building blocks that are arranged to interact through energy-dissipative interfaces. Examples of these materials include engineered constructions such as the Abeille vault and highly mineralized natural materials such as tooth enamel. Compared with synthetic materials, natural mate...
Article
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The induced pluripotent stem cell (iPSC) is a promising cell source for tissue regeneration. However, the therapeutic value of iPSC technology is limited due to the complexity of induction protocols and potential risks of teratoma formation. A trans-differentiation approach employing natural factors may allow better control over reprogramming and i...
Preprint
Full-text available
Materials with dense architectures are composed of stiff and strong building blocks that are arranged to interact through energy-dissipative interfaces. Examples of these materials include engineered constructions such as the Abeille vault and highly mineralized natural materials such as tooth enamel. Compared with synthetic materials, natural mate...
Article
Full-text available
In this work, we measured the mechanical properties and tested the cell viability of a bioceramic coating, strontium–hardystonite–gahnite (Sr–HT–G, Sr–Ca2ZnSi2O7–ZnAl2O4), to evaluate potential use of this novel bioceramic for bone regeneration applications. The evaluation of Sr–HT–G coatings deposited via atmospheric plasma spray (APS) onto Ti–6Al...
Article
Full-text available
Baghdadite (Ca3ZrSi2O9) (BAG) is a calcium silicate (CaSi) based ceramic that demonstrates osteostimulatory function, biocompability and chemical stability. This work studies atmospheric plasma sprayed (APS) BAG and APS hydroxyapatite (HAp) coatings by evaluating their phase compositions, chemical properties, coating microstructure and mechanical p...
Article
Mobility outcomes and changes in bone mineral density (BMD) of the spine and femoral necks in response to unilateral osseointegrated implants was investigated over a three year period. A total of 48 unilateral amputees who received an osseointegrated implant, comprising 33 trans‐femoral amputees (TFA) and 15 trans‐tibial amputees (TTA), underwent d...
Article
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Brushite cements have been clinically used for irregular bone defect filling applications, and various strategies have been previously reported to modify and improve their physicochemical properties such as strength and injectability. However, strategies to address other limitations of brushite cements such as low radiopacity or acidity without neg...
Article
Objectives: To examine periprosthetic bone remodeling among the recipients of 2 types of lower-limb osseointegrated systems, the Integral Leg Prosthesis (ILP) and the Osseointegration Prosthetic Limb (OPL) type A, over a >24-month period. Design: Retrospective cohort study. Setting: Private hospital, with a specialized osseointegration unit....
Article
Full-text available
Extracellular vesicles (EVs) are nanoscale particles secreted by almost all cell types to facilitate intercellular communication. Stem cell-derived EVs theoretically have the same biological functions as stem cells, but offer the advantages of small size, low immunogenicity, and removal of issues such as low cell survival and unpredictable long-ter...
Article
Achieving adequate healing in large or load‐bearing bone defects is highly challenging even with surgical intervention. The clinical standard of repairing bone defects using autografts or allografts has many drawbacks. A bioactive ceramic scaffold, strontium‐hardystonite‐gahnite or “Sr‐HT‐Gahnite” (a multi‐component, calcium silicate‐based ceramic)...
Article
Coating bioceramics of inherent bioactivity onto biometallic implants is a straightforward yet promising solution to address poor osteointegration of the latter. One step further, it would be a nontrivial accomplishment to develop a mild, cheap, and universal route to firmly stabilizing, in principle, any ceramics onto any implant substrate, while...
Article
The successful regeneration of functional bone tissue in critical‐size defects remains a significant clinical challenge. To address this challenge, synthetic bone scaffolds are widely developed, but remarkably few are translated to the clinic due to poor performance in vivo. Here, it is demonstrated how architectural design of 3D printed scaffolds...
Article
Injectable bone cement (IBC) such as those based on methacrylates and hydraulic calcium phosphate and calcium sulfate-based cements have been used extensively for filling bone defects with acceptable clinical outcomes. There is a need however for novel IBC materials that can address some of the inherent limitations of currently available formulatio...
Article
Injectable silk hydrogels are ideal carriers of therapeutic agents due to their biocompatibility and low immunogenicity. Injectable silk hydrogels for bone regeneration have been previously developed but often utilize expensive biologics. In this study, we have developed an injectable silk composite incorporated with a triphasic ceramic called MSM-...
Article
Full-text available
Extensive bone loss due to trauma or disease leads to impaired healing. Current bone grafts and substitutes have major drawbacks that limit their effectiveness for treating large bone defects. A number of bone substitutes in development are undergoing preclinical testing, but few studies specifically investigate the in vivo material–tissue interact...
Article
The successful regeneration of bone tissue to replace areas of bone loss in large defects or at load-bearing sites remains a significant clinical challenge. Over the past few decades, major progress is achieved in the field of bone tissue engineering to provide alternative therapies, particularly through approaches that are at the interface of biol...
Article
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Nature has the amazing ability to evolve materials with combined strength and toughness that far exceed those of their individual constituents. This study aims to develop a bioceramic that mimics the hierarchical organisation of the structure of nacre on multiple scale levels by using a widespread ceramics processing technique and bioactive compone...
Article
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Gehlenite (GLN, Ca2SiAl2O7) is a bioceramic that has been recently shown to possess excellent mechanical strength and in vitro osteogenic properties for bone regeneration. Substitutional incorporation of strontium in place of calcium is an effective way to further enhance biological properties of calcium-based bioceramics and glasses. However, such...
Article
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Scientific Reports 6 : Article number: 37909; 10.1038/srep37909 published online: 02 December 2016 ; updated: 07 March 2017 . The original version of this Article contained an error in the spelling of the author Jorge Luis Galeano Niño, which was incorrectly given as Jorge Luis Galenano-Niño.
Article
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Mesenchymal stem cells (MSCs) have been widely used for tissue repair and regeneration. However, the inherent drawbacks, including limited cell survival after cell transplantation, have hindered direct MSC transplantation for tissue repair and regeneration. The aim of this study was to investigate if exosomes isolated from MSCs can promote the prol...
Article
Full-text available
Doped calcium silicate ceramics (DCSCs) have recently gained immense interest as a new class of candidates for the treatment of bone defects. Although calcium phosphates and bioactive glasses have remained the mainstream of ceramic bone substitutes, their clinical use is limited by suboptimal mechanical properties. DCSCs are a class of calcium sili...
Article
Full-text available
Tissue engineering strategies to construct vascularized bone grafts are now attracting much attention. Strontium-hardystonite-Gahnite (Sr-HT-Gahnite) is a strong, highly porous, and biocompatible calcium silicate based bio-ceramic that contains strontium and zinc ions. Adipose derived stem cells (ASCs) have been demonstrated to have the ability in...
Article
Full-text available
The topography of a biomaterial regulates cellular interactions and determine stem cell fate. A complete understanding of how topographical properties affect cell behavior will allow the rational design of material surfaces that elicit specified biological functions once placed in the body. To this end, we fabricate substrates with aligned or rando...
Chapter
Full-text available
Stem cells play a critical role in tissue regeneration and repair, maintenance and turnover and the control of haematopoiesis in the various tissues. These cells have an incredible ability to differentiate into specific cell types like osteoblasts, chondrocytes or myocytes and to develop bone, cartilage or muscle tissues. Now it is believed that th...
Article
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Healing large bone defects, especially in weight-bearing locations, remains a challenge using available synthetic ceramic scaffolds. Manufactured as a scaffold using 3D printing technology, Sr-HT-Gahnite at high porosity (66%) had demonstrated significantly improved compressive strength (53 ± 9 MPa) and toughness. Nevertheless, the main concern of...
Article
Macrophages, the primary cells of the inflammatory response, are major regulators of healing, and mediate both bone fracture healing and the inflammatory response to implanted biomaterials. However, their phenotypic contributions to biomaterial-mediated bone repair are incompletely understood. Therefore, we used gene expression and protein secretio...