Hafida Merzouk

Hafida Merzouk
Abou Bakr Belkaid University of Tlemcen · Département de Biologie

Professor

About

129
Publications
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Publications

Publications (129)
Article
Introduction et but de l’étude La consommation du café est considérée comme bénéfique pour la santé. Parmi les composés actifs connus dans le café, les polyphénols protègent les cellules du stress oxydatif et ont des effets anti-inflammatoires. L’obésité avec excès de tissu adipeux est associée à un état inflammatoire chronique et à un stress oxyda...
Article
Full-text available
Highlights  Protective effect of linseed oil on lipid peroxidation and antioxidant activity was evaluated in pregnant and lacing overfed obese rats and their offspring  Linseed oil supplementation contribute to reduce body weight and lipid peroxidation in obese rats  Linseed oil supplementation significantly improves antioxidant status in diet-i...
Poster
Full-text available
Evaluation de l’activité antioxydante des pépins de raisin de l’Ouest Algérien DIDI A., MALTI N., MEJDOUB A., BOUDGHENE-GERRICHE A., BENAMAR A., DIDI M.A., MERZOUK H. Laboratoire des technologies de séparation et de purification, Département de chimie, Faculté des Sciences, Université AbouBekr Belkaid-Tlemcen-Algérie. ² Laboratoire de physiologie,...
Presentation
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INTRODUTION The present study was designed to confirm the idea that Bunium incrassatum tubers can be used as a source of nutraceuticals or as a functional food to reduce the risk of chronic diseases and as a diet by providing basic nutrition. The present study aimed at determining the effectiveness of Bunium incrassatum tubers' powder as dietary su...
Article
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Description of the subject: Maternal nutrition before and during pregnancy, is an easily modifiable environmental factor that can affect fetal growth and development with potential long-term consequences. The concept of «fetal programming» implies fetal life alterations expose to further developpment of pathologies. The research for new treatments...
Article
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A novel coronavirus responsible of acute respiratory infection closely related to SARS-CoV has recently emerged. So far there is no consensus for drug treatment to stop the spread of the virus. Discovery of a drug that would limit the virus expansion is one of the biggest challenges faced by the humanity in the last decades. In this perspective, to...
Article
In this study, the in vitro effects of 1-(4-dimethylaminobenzylidene)-2-(2-hydroxybenzylidene) hydrazone (L1) and its corresponding copper complex [Cu(L1)], synthesized in our laboratory, were investigated on the proliferative responses, Th1 (interleukin-2 (IL-2), interferon-γ (INFγ)) and Th2 (IL-4) cytokine secretion, adenosine triphosphate (ATP)...
Preprint
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A novel (COVID-19) responsible of acute respiratory infection closely related to SARS-CoV has recently emerged. So far there is no consensus for drug treatment to stop the spread of the virus. Discovery of a drug that would limit the virus expansion is one of the biggest challenges faced by the humanity in the last decades. In this perspective, tes...
Article
Objectives The pathogenesis of hypertension in diabetics is complex and involves several biological and environmental factors as well as a genetic predisposition; therefore, diabetic hypertension presents a greater risk of complications. In fact, 50% of diabetics suffer from hypertension. Diabetes is generally associated with increased oxidative st...
Article
Maternal nutrition is an easily modifiable environmental factor that can affect fetal growth and development with potential long-term consequences. The search for new treatments based on medicinal plants has turned to find the beneficial effects of olive oil on the pathogenesis of maternal obesity. Here, we studied whether maternal supplementation...
Article
Objective: To determine oxidative stress markers in maternal obesity during pregnancy and to evaluate feto-placental unit interaction, especially predictors of fetal metabolic alterations. Patients and methods: 40 obese pregnant women (prepregnancy BMI > 30 kg/m 2) were compared to 50 control pregnant women. Maternal, cord blood and placenta sample...
Article
In this study, plasma lipids, lipoproteins and markers of oxidant/antioxidant status were investigated in young (n Z 45) and older (n Z 40) obese men and compared to those in young (n Z 65) and older (n Z 55) normal weight controls. The purpose was to determine whether obesity exacerbates or not lipid, lipoprotein abnormalities and oxidative stress...
Article
Full-text available
It has long been established that maternal nutritional condition and fatty acid intake during pregnancy and/or lactation may influence the modifications in the individual risk for developing metabolic diseases throughout life, but little is known about the possible role of maternal nutrition before conception. The aim of this study was to investiga...
Article
Marine organisms, including microalgae, are among major subjects of investigation for the research of new molecules with biological activity that may improve responses to metabolic treatment. Microalgae Nannochloropsis gaditana are rich sources of diverse bioactive compounds. In this context, the present study was conducted to evaluate the effects...
Article
Background: The present work aims at determining the effects of maternal-diet-induced obesity on offspring metabolism. The short-term of a marine microalgae diet and its effects on lipids metabolism was investigated. Method: Before gestation, some rats are fed control diet and others cafeteria diet. Moreover, two groups of dams were fed standard...
Article
Background Physical activity has a positive influence on healthy state during menopause. Mediterranean diet is an indicator of healthy ageing. The aim of the work was to examine the effectiveness of one year aerobic training in the improvement of biochemical indices of lipid, glucose and oxidative stress status in postmenopausal women who totally a...
Article
The long-term nutritional efficacy of a highly-pure-enriched-cellulose (HPEC) diet and its effects on lipid metabolism and oxidant/antioxidant status were investigated in obese offspring of streptozotocin-induced diabetic rats. Diabetes in pregnant rats was induced by intraperitoneal injection of streptozotocin on day 5 of gestation. Control pregna...
Article
Maternal nutrition is an easily modifiable environmental factor that can affect fetal growth and developmentwith potential long-term consequences. The search for new treatments based on medicinal plants has turned to find the beneficial effects of olive oil on the pathogenesis of maternal obesity. Here, we studied whether maternal supplementation w...
Article
To investigate the possible developmental programming, we analyzed the effects of maternal and postnatal low dose alpha-cypermethrin exposure on metabolic and redox parameters in the offspring. Postnatal changes in plasma biochemical parameters and plasma and tissue oxidative stress markers were determined in offspring of dams fed standard chow or...
Article
Liver diseases are linked in the majority of cases to oxidative stress that antioxidants could neutralize with reducing liver injury. Chlorogenic acid, a coffee polyphenol, possesses antioxidant prosperities. The aim of this study was to evaluate in vitro preventive and corrective effects of cholorogenic acid in hepatocyte toxicity induced by free...
Article
Introduction et but de l’etude En raison de l’exigence accrue en oxygene et du placenta riche en mitochondries, la grossesse est une condition qui expose a une sensibilite aggravee au stress oxydatif. Cela a ete prouve par des etudes demontrant des niveaux eleves de marqueurs de stress oxydatif lors d’une grossesse normale. La periode perinatale et...
Article
Aging is an inevitable biological event that is associated with immune alterations. These alterations are related to increased cellular oxidative stress and micronutrient deficiency. Antioxidant supplementation could improve these age-related abnormalities. The aim of this study was to determine in vitro effects of vitamin A, vitamin C, vitamin E,...
Article
Full-text available
Background: Dietary fatty acids have important homeostatic functions in regulating the immune response and may exert beneficial effects on immune alterations during obesity. Objective: To assess the in vitro effects of oil fatty acids, different oils (olive, linseed, Nigel, sunflower) were tested on T-lymphocyte proliferation, Th1- and Th2-type cyt...
Data
Absorption spectrophotometric titration of quercetin by FeNTA. (A) Absorption spectra, (B) absorption electronic spectra, and (C) complex formation evolution as a function of the [FeNTA]0. Solvent: CH3OH/H2O (80/20 by weight); pH = 7.4 (Hepes buffer); T = 25.0(2°C; l = 1 cm; (1) [Quercetin]0 = 1.90 × 10−5 M; (2) [FeNTA]0/[Quercetin]0 = 5.58. (D) El...
Data
Absorption spectrophotometric titration of quercetin3OH by FeNTA. (A) Absorption spectra, (B) absorption electronic spectra, and (C) complex formation evolution as a function of the [FeNTA]0. Solvent: CH3OH/H2O (80/20 by weight); pH = 7.4 (Hepes buffer); T = 25.0(2°C; l = 1 cm. (1) [Quercetin3OH]0 = 1.51 × 10−5 M; (2) [FeNTA]0/[Quercetin3OH]0 = 2.4...
Data
Absorption spectrophotometric titration of quercetin35OH by FeNTA. (A) Absorption spectra, (B) absorption electronic spectra, and (C) complex formation evolution as a function of the [FeNTA]0. Solvent: CH3OH/H2O (80/20 by weight); pH = 7.4 (Hepes buffer); T = 25.0(2°C; l = 1 cm; (1) [Quercetin35OH]0 = 4.0 × 10−5 M; (2) [FeNTA]0/[Quercetin35OH]0 = 1...
Data
Absorption spectrophotometric titration of quercetin by Zn(II). (A) Absorption spectra, (B) absorption electronic spectra, and (C) complex formation evolution as a function of the [Zn(II)]0. Solvent: CH3OH/H2O (80/20 by weight); pH = 7.4 (Hepes buffer); T = 25.0(2°C; l = 1 cm. (1) [Quercetin]0 = 4.94 × 10−5 M; (2) [Zn(II)]0/[Quercetin]0 = 1.95. (DO...
Data
Absorption spectrophotometric titration of (+)catechin by Cu(II). (A) Absorption spectra, (B) absorption electronic spectra, and (C) complex formation evolution as a function of the [Cu(II)]0. Solvent: CH3OH/H2O (80/20 by weight); pH = 7.4 (Hepes buffer); T = 25.0(2°C; l = 1 cm. (1) [(+)Catechin]0 = 2.55 × 10−4 M; (2) [Cu(II)]0/[(+)Catechin]0 = 1.5...
Data
Absorption spectrophotometric titration of quercetin by Cu(II). (A) Absorption spectra, (B) absorption electronic spectra, and (C) complex formation evolution as a function of the [Cu(II)]0. Solvent: CH3OH/H2O (80/20 by weight); pH = 7.4 (Hepes buffer); T = 25.0(2°C; l = 1 cm. (1) [Quercetin]0 = 4.98 × 10−5 M; (2) [Cu(II)]0/[Quercetin]0 = 0.94. (DO...
Data
Absorption spectrophotometric titration of rutin by Cu(II). (A) Absorption spectra, (B) absorption electronic spectra, and (C) complex formation evolution as a function of the [Cu(II)]0. Solvent: CH3OH/H2O (80/20 by weight); pH = 7.4 (Hepes buffer); T = 25.0(2°C; l = 1 cm. (1) [Rutin]0 = 4.94 × 10−5 M; (2) [Cu(II)]0/[Rutin]0 = 1.40. (D) Electrospra...
Data
Absorption spectrophotometric titration of rutin by FeNTA. (A) Absorption spectra, (B) absorption electronic spectra, and (C) complex formation evolution as a function of the [FeNTA]0. Solvent: CH3OH/H2O (80/20 by weight); pH = 7.4 (Hepes buffer); T = 25.0(2°C; l = 1 cm. (1) [Rutin]0 = 4.94× 10−5 M; (2) [FeNTA]0/[Rutin]0 = 2.02. (D) Electrospray ma...
Data
Absorption spectrophotometric titration of rutin by Zn(II). (A) Absorption spectra, (B) absorption electronic spectra, and (C) complex formation evolution as a function of the [Zn(II)]0. Solvent: CH3OH/H2O (80/20 by weight); pH = 7.4 (Hepes buffer); T = 25.0(2°C; l = 1 cm. (1) [Rutin]0 = 4.94 × 10−5 M; (2) [Zn(II)]0/[Rutin]0 = 28.54. (DOCX)
Article
Full-text available
Natural flavonoids such as quercetin, (+)catechin and rutin as well as four methoxylated derivatives of quercetin used as models were investigated to elucidate their impact on the oxidant and antioxidant status of human red blood cells (RBCs). The impact of these compounds against metal toxicity was studied as well as their antiradical activities w...
Article
Full-text available
Introduction et but de l’étude L’obésité est considérée comme un facteur de risque durant la grossesse. À côté des désordres alimentaires, les mères obèses présentent une incidence élevée de troubles métaboliques et oxydatifs ainsi que des complications obstétricales, maternelles et fœtales. La grossesse tout comme l’obésité sont deux situations ca...
Research
this study was designed to determine whether maternal supplementation with olive oil influence plasma lipid profile and oxidant/antioxidant status later in life of rats with cafeteria- diet-fed during gestation and in their offspring throughout adulthood.
Article
Objectives: Deepen our knowledge of the immune system alterations associated with obesity-related hypertension and demonstrate that polyunsaturated fatty acids can enhance the proliferation and their profile oxidant/antioxidant and subsequently involved in the strategy prevention and treatment in obese hypertensives. Methods: T cells are isolate...
Article
The aim of this investigation was to determine the in vitro effects of vitamin C and E, n-3 and n-6 PUFA and n-9 MUFA on placental cell proliferation and function in type 1 diabetes. Placenta tissues were collected from 30 control healthy and 30 type 1 diabetic women at delivery. Placental cells were isolated and were cultured in RPMI medium supple...
Article
Pyrethroid insecticides are extensively used in agriculture and in household activities. During pregnancy, they might affect maternal metabolic status and there after fetal development. In this work, we studied metabolic and redox effects of low dose alpha-cypermethrin exposure in pregnant rats and their offspring. The diet containing alpha cyperme...
Article
Full-text available
Objective: Hypothyroidism has been found to increase oxidative stress parameters and to disturb lipid profiles. The aim of this study was to determine the effect of high thyroid stimulating hormone level (TSH) on lipid profile and oxidative stress biomarkers in women particularly nitric oxide (NO •) and superoxide anion (O2 •-). Patients and method...
Article
Full-text available
Objective: The aim of the present work was to study how maternal dietary linseed oil modulates lecithin cholesterol acyltransferase (LCAT) and adipose tissue lipase responses to cafeteria diet in rats during pregnancy and lactation and their offspring at weaning and adulthood. Methods: The dams were fed a control (C) or a Cafeteria (CAF) diet enric...
Article
The objective of this study was to determine linseed oil's effect on oxidative stress parameters and lipid profile in three generations of offspring of obese rats. The initial age of the female rats is two months and of a weight of 100 ± 10 g were fed for two months with a control diet or a cafeteria diet, fortified or not with linseed oil (5%). At...
Article
The aim of this work was to investigate inflammatory, oxidative, and thrombotic parameters as biomarkers in farmers exposed to pesticides. Fifty farmers using chemical pesticides and 60 unexposed control men participated in this study. The Mediterranean diet compliance, the duration of pesticide use, and personal protection for pesticides handling...
Article
Obesity is characterized by iron deficiency, carbohydrate and fat alterations as well as oxidative stress. Iron status monitoring is recommended because of the conventional oral iron preparations that frequently exacerbate the already present oxidative stress. Iron complexation by natural antioxidants can be exploited. We herein investigated the me...
Article
Objectifs: Notre société est confrontée à des taux croissants de maladies métaboliques telles que l'obésité, l'hypertension artérielle et les pathologies cardiovasculaires. L'obésité liée à l'hypertension s'accompagne de modifications importantes du système immunitaire. Il est généralement admis que les AGPI n-3 développent des activités immunomod...
Article
Objectifs: L'HTA est à l'origine de plus de la moitié des cas d'insuffisance rénale terminale. À la fois cause et conséquence, cette maladie est également une complication extrêmement fréquente en cas de dégradation continue des reins. Le stress oxydant est de plus en plus étudié tant dans le domaine de la recherche qu'en médecine humaine. Il a ét...
Article
IRON-QUERCETIN COMPLEXES REDUCE LIPIDE PEROXYDATION AND ENHANCE CATALASE ACTIVITY IN ORGANS TARGET OF DIABETES COMPLICATIONS IN RATS Farid BERROUKECHE, Nassima MOKHTARI-SOULIMANE, Asmahan IMASSAOUDEN, Sabah GUEDDOUCHI, Hafida MERZOUK, Mourad ELHABIRI Université de Tlemnce, Algeria; Laboratory of chemistry, bio- organic and medicinal, Strasbourg Uni...
Article
Full-text available
To investigate the oxidative profile and thrombotic markers in obese and hypertensive mothers. Thirty obese, 28 hypertensive and 34 healthy control mothers were recruited from Tlemcen Hospital, Algeria. Plasma vitamin C, nitric oxide, superoxide anion, erythrocyte glutathione, malondialdehyde, carbonyl proteins and erythrocyte antioxidant enzyme ac...
Article
Full-text available
Because linseed oil may influence maternal and fetal metabolisms, we investigated its role in the modulation of lipid metabolism in cafeteria diet-induced obese rats and their offspring. Female Wistar rats were fed control or cafeteria food, which were either supplemented or not supplemented with linseed oil (5%) for 1 month before and during gesta...
Article
Full-text available
The effects of calcium antagonists (amlodipine) and angiotensin II receptor antagonists (telmisartan) on lipid profile and oxidative markers are investigated in Algerian hypertensive patients. At the beginning and after one year of antihypertensive therapy, blood samples are collected for biochemical parameters (glucose, cholesterol, triglycerides,...
Article
Background The aim of this investigation was to determine the in-vitro effects of linseed, olive and Nigel oils on T cell proliferation and function in gestational diabetes.Methods Blood samples were collected from 40 control healthy and 32 gestational diabetic mothers and their newborns. Peripheral blood lymphocytes were isolated using a density g...
Article
Full-text available
This work focused on the study of the in vitro effects of different concentrations of copper and nickel complexes of 2,5-bis(2-pyridyl)-1,3,4-thiadiazole on the proliferative responses of human mitogen-stimulated lymphocytes, the Th1-(IL-2, INFγ) and Th2-(IL-4) cytokine secretion and the intracellular oxidant/antioxidant status. The results showed...
Article
Microtubules are major constituents of the cytoskeleton and are involved in many cellular processes such as the formation of the mitotic spindle and intracellular trafficking. These tubular structures, composed by tubulin α/β heterodimers assemble and disassemble with a finely regulated dynamics. Stathmin is a cytosolic phosphoprotein that sequeste...
Article
Full-text available
The aim of this work was to determine the effect of dietary n - 3 PUFA on oxidant/antioxidant status, in vitro very low and low density lipoprotein (VLDL-LDL), and VLDL-LDL-fatty acid composition in macrosomic pups of diabetic mothers. We hypothesized that n - 3 PUFA would improve oxidative stress in macrosomia. Diabetes was induced in female Wista...
Article
Full-text available
Diabetes mellitus is a predominant chronic disease which causes mortality of millions of people yearly. Its prevalence is on the rise worldwide. Water kefir is fermented food produced by a matrix of polysaccharides containing bacteria and yeasts, with therapeutic properties. Our study aimed to evaluate anti-hyperglycemic and anti-hyperlipidemic act...