Hadri Latif

• Dr
• Senior Researcher at IPB University

The rapid growth of the dairy industry has led to increased antibiotic use in dairy cattle, causing a surge in antibiotic-resistant genes. This, in turn, has expedited bacterial resistance development. The objective of this research was to analyze the presence of beta-lactam antibiotic resistance genes in E. coli derived from dairy cattle manure in...

Importance: The emergence and rapid increase in the incidence of multidrug-resistant (MDR) bacteria in pig farms has become a serious concern and reduced the choice of effective antibiotics. Objective: This study analyzed the phylogenetics and diversity of antibiotic resistance genes (ARGs) and molecularly identified the source of ARGs in antibi...

Contamination of Extended spectrum-betha lactamase (ESBL) producing Escherichia coli in milk can cause public health problems. The misuse of antibiotics in dairy has implications for the emergence of multi-resistant bacteria that can infect humans through food (foodborne diseases). The ESBL producing E. coli is resistant to ?-lactam antibiotics (in...

Background and Aim Livestock waste in the form of feces and liquid represents an important reservoir of antibiotic resistance genes (ARGs). Because many ARGs can be horizontally transferred to other pathogens, livestock waste plays an essential role in the emergence and transmission of various ARGs in the environment. Therefore, this study aimed to...

Kadar nitrit dalam sarang burung walet (SBW) telah menjadi perhatian dalam beberapa tahun terakhir. SBW yang diekspor dari Indonesia ke Negara Tiongkok harus memenuhi standar kadar nitrit (NO2), yaitu maksimum 30 ppm. Dinamika perkembangan teknologi dan jaman saat ini menuntut instrumen pengujian kadar nitrit secara akurat, diantaranya menggunakan...

Background and aim: Slaughterhouses and their effluents could serve as a "hotspot" for the occurrence and distribution of antibiotic-resistant bacteria in the environment. This study aimed to understand the distribution of tetracycline resistance genes in Escherichia coli isolated from the floor surface and effluent samples of pig slaughterhouses...

Burung walet di Indonesia umumnya dibudidayakan pada rumah burung walet (RBW) secara tradisional dengan pola pemeliharaan tertentu. Pola pemeliharaan menjadi salah satu faktor pendukung bagi burung walet untuk memproduksi sarang burung walet (SBW) dengan kualitas baik secara berkelanjutan. Penelitian ini bertujuan untuk menganalisis pola pemelihara...

Background and aim: Since the past decade, metagenomics has been used to evaluate sequenced deoxyribonucleic acid of all microorganisms in several types of research. Nitrite contamination originates from the natural environment in Swiftlet farmhouses (SFHs) and can influence nitrite levels in edible bird's nest (EBN). It is strongly speculated tha...

Background and aim: In 2020, Indonesia, which has the highest global production of edible bird's nest (EBNs), exported up to 1312.5 tons of this product at a value of USD 540.4 million. Recently, food safety aspects related to EBNs, including contamination with heavy metals, have become a serious concern. However, data on the presence and concentr...

Latar belakang: Escherichia coli merupakan bakteri enterik komensal yang mudah resistan terhadap antibiotik karena memiliki kemampuan bawaan dalam menghasilkam enzim resistansi antibiotik, salah satunya Extended Spectrum Beta Lactamase (ESBL). Bakteri ini dapat ditularkan melalui lingkungan, salah satunya melalui cemaran efluen dari rumah potong he...

Amoeba that infects primates can also be found in humans and can be zoonotic. This study aims to identify the distribution of intestinal amoeba in the wild Long-Tailed Macaque (LTM) group located at Cot Murong, Kebun Mangga, and Tugu Zero Kilometer Monument (IZKM) in Sabang City, Aceh Province, Indonesia. A total of 177 stool samples from 59 tails...

Edible bird nest is a high-value export commodity. The industry of edible bird nests encounters various challenges regarding food safety demands for consumers, especially related to the quality of edible bird nests and compliance of nitrite below 30 ppm for the export commodity to China. The purpose of this paper is to obtain information on nitrate...

Salbutamol, a short-acting β2-adrenergic receptor agonist, due to illegal use in animal feed, is very dangerous for the safety of animal origin products. The purpose of this study is to determine the residue of salbutamol in plasma and urine of balinese calf after a single-dose administration. Three calves were given of feed fortification of salbut...

Beef offal are consumed by people in some countries specifically in Asia. Beef liver and lungs are favorite food which are used as meat in traditional food. The objective of this study was to determine the postmortem changes in pH, color, drip loss, and non-protein nitrogen (NPN) content in beef liver and lungs during storage in refrigerator (3-4 º...

Background and aim: Campylobacter species have been recognized as the most frequently identified bacterial cause of human gastroenteritis. The aims of this study were to identify Campylobacter jejuni and Campylobacter coli species isolated from chicken meat and to analyze the differences in the melting curve patterns of both species. Materials an...

Sampah yang berasal dari pesawat internasional yang datang di Indonesia harus dimusnahkan tanpa kecuali dan berlaku untuk penerbangan dari negara manapun. Pemusnahan dilakukan untuk menghindari risiko penyebaran penyakit dari sampah yang dapat menyerang hewan, tumbuhan dan manusia. Aktivitas penumpang dari mancanegara, migrasi orang dan hewan dari...

Amoebiasis caused by Entamoeba histolytica is an important issue in world public health because it is associated with high morbidity and mortality. Entamoeba histolytica is the only species of its genus that commonly causes mild irritation, injury, to inflammation of the walls of the colon and cecum. In some cases, parasites also invade other organ...

Penelitian ini bertujuan untuk mengidentifikasi dan menganalisis penambahan daging babi ternak dan babi hutan baik yang mentah (raw) maupun yang diolah (cooked) di dalam pangan asal hewan berbahan dasar daging sapi menggunakan metode uji enzyme-linked immunosorbent assay (ELISA) dan real-time PCR (qPCR). Sebanyak 40 sampel yang terdiri dari 20 dagi...

Extended spectrum β-lactamase (ESBL) adalah enzim yang dapat menghidrolisis berbagai jenis antibiotik β-laktam termasuk generasi ketiga sefalosporin spektrum luas dan monobaktam. Tujuan penelitian ini adalah untuk melihat kepekaan dan resistensi Escherichia coli penghasil ESBL terhadap beberapa antibiotik. Isolat Escherichia coli penghasil ESBL (n=...

Brucellosis has become a zoonotic disease that received attention in efforts to prevent and eradicate strategic infectious animal diseases in Indonesia. Brucellosis can be detected early by the rose bengal test (RBT), followed by complement fixation test (CFT) and by enzyme linked immunosorbent assay (ELISA). The aims of this research was to study...

Aim: Meat authentication gives significance values in view of religious, food safety, public health, quality assurance, and legal concern. Most of the meat authentication is based on molecular assay; a simpler method to authenticate meat is needed to develop. An immunoassays technique may offer a solution for simpler test. The aim of our current s...

Penelitian ini bertujuan untuk mengukur tingkat pengetahuan dan sikap kader posyandu sebelum dan setelah intervensi. Penelitian ini juga membandingkan perbedaan metode ceramah dan buzz grup dalam meningkatkan pengetahuan dan sikap kader. Penelitian ini menggunakan rancangan quasi eksperimen dengan pre-test and post-test design. Sampel terdiri dari...

High population of Sumateran wild boar causes the increasing number of hunting and overflowing of wild boar meat availability in the market at a very low price. This condition leads to the falsification of meat. This study was aimed to characterize and differentiate Sumateran wild boar meat (Sus scrofa vittatus) and beef (Bos taurus) since Sumatera...

Objective: To determine the multidrug resistance extended spectrum β-lactamase and AmpC (ESBL/AmpC producing) Escherichia coli (E. coli) isolated from the environment of Bogor slaughterhouse, Indonesia. Methods: A total of 35 samples from 7 locations in slaughterhouse i.e., source of water, slaughtering floor, swab of carcass area floor, swab of ev...

The use of antibiotics in food-producing animals may encourage the occurence of bacterial resistance named Escherichia coli that produces Extended Spectrum β-lactamase (ESBL). Antibiotical resistance is an important issue in the animal health and human health. This study focused on the presence of ESBL-producing E.coli in chicken feces. The number...

This study was aimed to determine the occurrence of CTX-M-15 and CTX-M-55 producing Escherichia coli isolated from milk samples in West Java, Indonesia. A total of 129 milk samples were collected from diary farms from March to April 2016. Presence of extended-spectrum beta-lactamase (ESBL) producing E. coli was detected by disc diffusion test based...

This study was aimed to determine the occurrence of CTX-M-15 and CTX-M-55 producing Escherichia coli isolated from milk samples in West Java, Indonesia. A total of 129 milk samples were collected from diary farms from March to April 2016. Presence of extended-spectrum beta-lactamase (ESBL) producing E. coli was detected by disc diffusion test based...

Cortisol concentration in blood serum can be made as an indirect indicator to measure the level of stress in cattle. Cattle can experience stress when faced with something frightening and uncomfortable, such as rough handling before slaughter. The aim of this research is to assess the stress level of beef cattle by measuring the cortisol concentrat...

This study was aimed to determine the occurrence of CTX-M-1 and CTX-M-55 producing Escherichia coli isolated from broiler feces in Bogor, West Java, Indonesia. A total of 200 broiler fecal samples were collected from poultry slaughterhouses from March to April 2016. Presence of extended-spectrum beta-lactamase (ESBL) producing E. coli was detected...

Highly sensitive and specific coagglutination using crystal violet staining for detection of Escherichia coli 0157 H7 was introduced. In this method, crystal violet was used to stain the Staphylococcus aureus cells that have much of protein A. This staining acts as a detector reagent in this assay. The antibody immobilized with stained S. aureus pr...

Penelitian ini bertujuan untuk menganalisis keberadaan daging ilegal di perbatasan darat Indonesia-Malaysia sebagai ancaman risiko masuknya virus PMK ke wilayah Indonesia. Data primer diambil menggunakan teknik pengumpulan pendapat pakar dengan kuisioner , wawancara mendalam ( in-depth interview ) dan pengamatan langsung di lapang. Data sekunder di...

This study was aimed to determine the influence of the washing method under running water on nitrite levels of edible bird’s nest (EBN). Total of 40 samples of EBN were divided into four groups with different washing frequency, control group without washing treatmet (P0), once, twice, and three times washing treatment (P1, P2, and P3) respectively....

This study aimed to determine the presence of Salmonella spp. in duck meat and to identify the resistance of Salmonella spp. against several antibiotics in duck meat associated with food safety. Total of 52 meat samples of ducks was collected from 5 subdistricts in Bogor District, i.e., Cariu-Jonggol, Gunung Sindur, Klapanunggal, Parung Panjang, an...

This research was aimed to examine the heating effect at 70 C for 3.5 seconds on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) decontamination in edible bird´s nest. This study used 40 clean edible bird´s nest samples. Samples were divided into two groups, first group was contaminated with E. coli and second group was contaminat...

This study was conducted to investigate the presence of shiga toxin producing Escherichia coli (STEC) and the possibility of carrying rfbE gene and H7 flagellar on meat, liver, and stool samples collected from Jakarta Province of Indonesia. A total of 51 samples collected from meat, liver, and stool of slaughtered cattle from qurban festival were t...

Objective: To determine the occurrence of CTX-M producing Escherichia coli (E. coli) from cattle feces in Bogor slaughterhouse, Indonesia. Methods: A total of 220 cattle feces samples were collected from Bogor slaughterhouse from March to April 2015. Presence of extended-spectrum beta-lactamase (ESBL) producing E. coli was detected by disc diffusio...

Staphylococcus aureus is known as a major causative agent of mastitis in dairy cattle. In the presentstudy, 104 isolates of Staphylococcus originated from subclinical mastitis cattle characterized for thephenotypic properties and the presence of Staphylococcal protein A (Spa). Some bacteria were resistancesagainst several antibiotics were also stud...

p>Trenbolon asetat (TBA) merupakan hormon penggertak pertumbuhan yang diimplankan ke sapi untuk meningkatkan berat badan dan mengefisiensi konversi pakan. Penggunaan TBA dapat meninggalkan residu dalam urin dan dapat menyebabkan efek negatif. Tujuan dari penelitian ini adalah untuk menganalisis keberadaan residu TBA dalam urin sapi siap potong impo...

p>Parameter untuk mengetahui hewan sapi sempurna setelah disembelih yaitu dengan melihat refleks kelopak mata dan atau waktu henti darah memancar. Menurut EFSA (2004) kematian merupakan suatu keadaan yang ditandai dengan respirasi fisiologis dan sirkulasi darah telah berhenti sebagai akibat dari pusat sistem tersebut di batang otak secara permanen...

Zeranol is one of the synthetic growth hormone produced from mycotoxin that could affect humanhealth. The objective of this study was to determine zeranol residue in beef meat imported from Australiaand New Zealand using Enzyme-Linked Immunosorbent Assay (ELISA). The results showed that zeranolresidue was detected in 5 of 59 meat samples (8,5 %) of...

The ergoline alkaloid fumigaclavine A (FuA) is one of the major mycotoxins produced by Aspergillus fumigatus, the main causative fungal agent of avian aspergillosis. To study in situ production of FuA, post-mortem respiratory tissues of various avian species, as well as blood samples of falcons (Falco sp.), were analysed by enzyme immunoassay (EIA)...

ABSTRAK Parameter untuk mengetahui kesempurnaan kematian pada sapi setelah disembelih yaitu dengan melihat refleks kelopak mata dan atau waktu henti darah memancar. Menurut EFSA (2004) kematian merupakan suatu keadaan yang ditandai dengan respirasi fisiologis dan sirkulasi darah telah berhenti sebagai akibat dari pusat sistem tersebut di batang ota...

This study was aimed to describe cortisol hormone concentration and meat quality which were produced in beef cattle stunned with captive bolt stun gun before slaughtering. Blood and meat samples were taken from Brahman cross steers (n= 11) in two abattoirs located in West Java and Banten. Blood samples were collected immediately after slaughtering....

Tujuan penelitian ini adalah untuk menentukan komposisi kimiawi dan fraksinasi senyawa protein susu kuda sumba. Penentuan komposisi kimiawi susu kuda sumba dilakukan melalui pengujian kadar protein susu menggunakan metode Kjeldahl, kadar lemak susu menggunakan metode Gerber, kadar laktosa dan kadar total padatan. Identifikasi senyawa senyawa antimi...

The aims of this study were to fractionate and to identify antimicrobial activity of Sumba mare's milk protein against causative agent of subclinical mastitis. Antimicrobial compounds isolation of whey protein were fractionate using alkaline alumina column. Determination of antimicrobial activity of protein fractions through suseptibility test agai...

The Alternaria mycotoxin tenuazonic acid was derivatized with succinic anhydride and conjugated to keyhole limpet hemocyanin (KLH) and to horseradish peroxidase (HRP), respectively. The KLH conjugate was used to produce polyclonal antibodies in rabbits. A competitive direct enzyme immunoassay (EIA) for tenuazonic acid was established, which was mod...

This study investigated the production of polyclonal (pAB) antibodies and the first time production of monoclonal (mAB) antibodies against the mycotoxin alternariol, and their implementation in enzyme immunoassay (EIA) for the rapid determination of alternariol in foods. Both EIAs were highly sensitive, with detection limits (IC₂₀) of 35 ± 6.9 pg/m...

Polyclonal antibodies against fumigaclavine A (FuA) were obtained from rabbits after immunization with a FuA-keyhole limpet hemocyanine conjugate prepared by formaldehyde condensation. Using these antibodies and a FuA-bovine serum albumine conjugate, a competitive indirect enzyme immunoassay (EIA) was established. The antiserum obtained from one ra...

The present study describes the development of specific polyclonal antibodies against fumigaclavine A (FuA) in rabbits, and the development of a highly sensitive enzyme immunoassay (EIA) for this mycotoxin. The Mannich condensation reaction with formaldehyde was used to conjugate FuA to keyhole limpet hemocyanin (KLH) as the immunogen, and to bovin...