Hadda Imene Ouzari

Hadda Imene Ouzari
University of Tunis El Manar | FST · Department of Biology

Professor

About

175
Publications
30,886
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
2,409
Citations
Introduction
Dr. OUZARI Hadda-Imene is a professor of Microbiology and Director of the laboratory of Microorganisms and Actives Biomolecules at the Faculty of Sciences of Tunis, University Tunis El Manar. The main research and teaching activities of Prof. Ouzari are focused microbial ecology: Biotechnological potentialities and applications of microbes from different environments with special focus on microorganisms from extreme environments and beneficial microbes in argo-food and health related sector.

Publications

Publications (175)
Article
Full-text available
Objectives: Human leukocyte antigen-G (HLA-G) is implicated in several cancers and is considered to be an immune checkpoint regulator. We determined the association between polymorphisms in the 3' untranslated region of HLA-G and soluble HLA-G (sHLA-G) expression with gynecological cancers (GCs). Methods: A meta-analysis was conducted to examine...
Article
Full-text available
Background /Objective: Conflicting results on the association between HLA-G and digestive cancers were reported. We conducted a meta-analysis to further investigate the true relationship between HLA-G and digestive cancers (DC). Methods Following PRISMA guidelines, we performed a meta-analysis including 7 case-control studies on HLA-G 14-bp Insert...
Article
Background: Human leukocyte antigen-G (HLA-G) gene polymorphisms and circulating sHLA-G have often been linked to the risk of breast cancer (BC). However, the results remain controversial. To resolve this issue, we performed a meta-analysis of HLA-G gene polymorphisms and sHLA-G levels in BC. Methods: We performed a meta-analysis on the associat...
Article
Full-text available
In Algeria, vaccination against pertussis is carried out using the whole-cell pertussis vaccine combined with the diphtheria and tetanus toxoids (DTwp). The quality control of vaccines locally produced or imported is carried out before the batch release. The aim of our work was to evaluate the potency of pertussis vaccines. In the present study, fi...
Article
Full-text available
Ticks are one of the most important vectors of several pathogens affecting humans and animals. In addition to pathogens, ticks carry diverse microbiota of symbiotic and commensal microorganisms. In this study, we have investigated the first Tunisian insight into the microbial composition of the most dominant Hyalomma species infesting Tunisian catt...
Article
Full-text available
Abstract Weissella strains have been reported to be useful in biotechnological and probiotic determinations, and some of them are considered opportunistic pathogens. Given the widespread interest about antimicrobial susceptibilities, transmission of resistances, and virulence factors, there is little research available on such topics for Weissella....
Article
Full-text available
Discovering new species and interesting bioactive metabolites from customary sources is becoming progressively laborious. Propolis constitutes the largest diversified reserve of microbial constituents in the beehive. However, fungal communities associated with these environments remain insufficiently established. We present the first detailed inves...
Article
In this study, we achieve the production of nontoxic Cu2Fe1-xAlxSnS4 films (x= 0, 0.25, 0.50, 0.75 and 1) by substituting Fe with Al atoms. Physical properties of the investigated films were studied using: Energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), X-ray diffraction (XRD), Raman spectroscopy, spectrophotometer a...
Preprint
Polycyclic aromatic hydrocarbons (PAHs) are being considered as substances of potential human health hazards due to their resistance toward biodegradation and carcinogenic index. Chrysene is a high molecular weight (HMW)PAHs; the biodegradation of Chrysene using bacteria is recognized as an efficient, environmentally friendly, and cost-effective ap...
Article
This study aimed to investigate the molecular mechanisms of carbapenem and colistin resistance in K. pneumoniae and E. coli isolates obtained from hospitalized patients in Carthagene International Hospital of Tunis. A total of 25 K. pneumoniae and 2 E. coli clinical isolates with reduced susceptibility to carbapenems were recovered. Susceptibility...
Article
This study aims to identify and determine the prevalence of Yersinia enterocolitica in ground beef meat during conservation process. One hundred eighty sample‐units of ground beef from three red‐meat processing companies were subjected to microbial analysis. The identification and prevalence rate of Y. enterocolitica in ground beef were performed u...
Patent
Full-text available
La présente invention se rapporte à un procédé d'élimination des contaminants des eaux, spécialement le colorant textile bleu Cibacron, et le bleu basique par des peptides fixateurs de colorants seront détaillés comme exemples. Dans le contexte de l'invention, le traitement de l’eau colorée comprend au moins une étape de sélection de peptides cible...
Conference Paper
Objectives It is widely accepted that HLA-G belongs to the group of checkpoint molecules, implicated in different pathways of immune suppression allowing tumors escape. Here, we investigated HLA-G expression in vulvar squamous cell carcinoma (VSCC) to explore the potential implication of these molecules as prognostic markers. Methods Immunohistoch...
Conference Paper
Full-text available
Objectives HLA-G gene polymorphisms have been linked to many cancers particularly to cervical squamous cell carcinoma (CSCC). In this meta-analysis, we studied the association of HLA-G +3142 C/G and 14bp Insertion/deletion (Ins/Del) polymorphisms with cervical pathologies susceptibility. Methods A comprehensive systematic literature search in Medl...
Article
Human leukocyte antigen (HLA)-G has been considered as an immune modulator in several types of cancers. Its genetic polymorphisms may potentially affect the risk of developing colorectal cancer (CRC). The overall purpose of this study was to analyze the implication of HLA-G 3′untranslated region (3′UTR) polymorphisms particularly 14 pb insertion/de...
Article
Objectives Colorectal cancer (CRC) is a serious gastrointestinal disease. Cancer cells can survive in a microenvironment that includes distinct immune cells, various immune checkpoints including HLA-G, and different immune effectors such as interleukin-6 (IL-6), TNF-alpha (TNF-α), and interferon-gamma (IFN-γ). Thus, the objective of this study was...
Article
Full-text available
Background: Human leukocyte antigen (HLA)-G is a non-classical major histocompatibility complex class Ib molecule. Its expression has been described in various cancer types, including ovarian cancer. HLA-G molecule has been implicated in immune escape and in progression of ovarian tumor cells. Our goal was to assess if total soluble (s)HLA-G molec...
Article
Full-text available
Aridity negatively affects the diversity and abundance of edaphic microbial communities and their multiple ecosystem services, ultimately impacting vegetation productivity and biotic interactions. Investigation about how plant‐associated microbial communities respond to increasing aridity is of particular importance, especially in light of the glob...
Article
Citrobacter freundii has acquired resistance to several antimicrobial drugs, including last-resort antibiotics affecting, therefore, clinical efficacy and causing high rates of mortality. In this study, we investigate the whole genome sequence of a carbapenem-resistant C. freundii strain isolated from the hospital environment in Tunisia. A total of...
Article
Full-text available
The adverse health effects of benzene occupational and circumstance pollution exposure are an increasing concern. It leads to damage to various human tissues including bone marrow and ovarian tissues and many vital physiological processes. Previous studies showed that kefir is a rich probiotic, having protective effect, thanks to its antioxidant, a...
Article
Full-text available
The bacterial genus Pantoea has been widely evaluated as promising bacteria to increase phos-phorus (P) availability in soil. The aim of this study was to characterize the phosphate solubiliz-ing (PS) activity of a Pantoeaagglomeransstrain and to evaluate the impact of its application in a semi-arid soil on phosphate availability and structure of t...
Article
Wastewater contaminated with the antibiotic-resistant bacteria, Pseudomonas aeruginosa, can contribute to human community-acquired infections when released into receiving waters. This study outlines a novel process of phage application that can reduce the reservoir of P. aeruginosa in both primary wastewater (PWW) and secondary wastewater (SWW). Th...
Article
Full-text available
A thorough assessment of the phylogenetic diversity and community structure of halophilic archaea from three halite-crystal salts, processed from two separated saline systems of Southern Tunisia has been performed using culture dependent and independent methods targeting different regions of 16S rRNA gene sequences including DGGE, 16S rRNA clone li...
Article
Co doped La2O3 thin films are prepared successfully by the spray pyrolysis method. These thin layers are characterized by different techniques. First, the structural and the morphological properties are dtudied by XRD, Raman and SEM techniques. Second, the photoluminescences (PL) measurements are carried out on these films and tested for the photoc...
Article
The use of starter cultures in the industrial manufacture of meat products has become common practice. We have investigated the use of Lactobacillus sakei F, isolated from a commercial starter culture, and Lactobacillus sakei 23K, from a French sausage, alone or together with Staphyloccus xylosus, as starter cultures during the industrial manufactu...
Article
The present study was intended toward the optimization of a textile dye Novacron Red decolorization by single and mixed culture of Bacillus strains namely, B. firmus, B. filamentosus and B. subterraneus. Optimization of dye decolorization using Bacillus monocultures was conducted using central composite design. The maximum dye decolorization achiev...
Chapter
Full-text available
Members of extremely halophilic archaea, currently consisting of more than 56 genera and 216 species, are known to produce their specific bacteriocin-like peptides and proteins called halocins, synthesized by the ribosomal pathway. Halocins are diverse in size, consisting of proteins as large as 35 kDa and peptide “microhalocins” as small as 3.6 kD...
Article
High molecular weight dextrans improve the rheological properties of fermented products and have immunomodulatory and antiviral activity. We report on 5.84 × 107–2.61 × 108 Da dextrans produced by Leuconostoc lactis AV1n, Weissella cibaria AV2ou and Weissella confusa V30 and FS54 strains. Dextransucrases catalyze dextran synthesis by sucrose hydrol...
Article
Full-text available
For decades, lactic acid bacteria has been isolated and selected to be used as starter cultures in meat fermentation for standardization and management of quality of dry‐fermented sausage which constitute a considerable challenge. The aim of this study was to evaluate the effect of Lactobacillus sakei strains, isolated from different origins, on qu...
Article
Full-text available
Streptomyces is the most frequently described genus of Actinomycetes, a producer of biologically active secondary metabolites. Indeed, the Streptomyces species produces about 70% of antibiotics and 60% of antifungal molecules used in agriculture. Our study was carried out with the goal of isolating and identifying antimicrobial secondary metabolite...
Article
Background Determination of BRAF status is mandatory for targeted therapy but it is not currently available in most developing countries. Aim We aimed to analyze BRAF mutations in a series of cutaneous melanoma of Tunisian patients and to compare BRAF V600E mutation detection by immunohistochemistry to DNA sequencing. Patients and Methods Archiva...
Article
HLA-G has been widely implicated in advanced cancers through different pathways of immunosuppression allowing tumor escape. Contrarily, HLA-E has a controversial role in the tumor escape from the immune system. IDO catabolic enzyme is known to be up-regulated in many tumors types allowing their immune escape. Based on these considerations, we inves...
Article
A new bis(benzamidinium)tetrachloridomercurate(II) monohydrate, (C 7 H 9 N 2) 2 [HgCl 4 ]ÁH 2 O, was synthesized by slow evaporation at room temperature and characterized by powder XRD, IR, Raman, UV-vis spectroscopy and thermal analysis. Single-crystal X-ray dif-fraction analysis indicates that the asymmetric unit of the title compound is formed b...
Article
A new bis(benzamidinium)tetrachloridomercurate(II) monohydrate, (C7H9N2)2[HgCl4]H2O, was synthesized by slow evaporation at room temperature and characterized by powder XRD, IR, Raman, UV-vis spectroscopy and thermal analysis. Single-crystal X-ray diffraction analysis indicates that the asymmetric unit of the title compound is formed by tetrachlori...
Article
Full-text available
This study explored the possible use of a microbial carboxymethylated sulfated heteroexopolysaccharide (CS-hEPS) as a potential anticancer agent. The investigation was carried out through antioxidant, antifatigue, and antiproliferative activities. Antioxidant potential including scavenging DPPH and hydroxyl radical activities and reducing power was...
Article
Full-text available
A hybrid pilot-scale constructed wetland (HSSF-FWS) with three horizontal flow components and a free water surface in series (HF-CW1 + HF-CW2 + HF-CW3 + FWS) was designed and investigated to evaluate seasonal wastewater treatment performance, the microbial diversity and community structure. The HF-CW1 component was planted with Scirpus holoschoenus...
Article
Factors affecting the removal of opportunistic pathogens were evaluated in a hybrid constructed wetland (CW2) treating rural wastewater. The correlations between the bacterial population and the environmental variables showed that bacteria discharge was the result of abiotic factors change such as temperature (T), electrical conductivity (EC), diss...
Article
Purpose The agri-food industry generates significant amounts of byproducts, among them repasso olive pomace (ROP) which are rejected and can constitute a serious environmental problem. Our study aimed to investigate the diversity of microbiota isolated from ROP and screen for their biotechnological potentials. Methods A collection of 102 strains (...
Article
Metallo-β-lactamase (MBL) producing bacteria constitute nowadays a serious global concern worldwide. The purpose of our present study was to characterize molecular features of MBL producing bacteria and to identify the existing clones in our area. Thirteen MBL-producing-Klebsiella pneumoniae were detected in clinical samples from patients hospitali...
Article
Full-text available
Metallo-β-lactamase (MBL) producing bacteria constitute nowadays a serious global concern worldwide. The purpose of our present study was to characterize molecular features of MBL producing bacteria and to identify the existing clones in our area. Thirteen MBL-producing-Klebsiella pneumoniae were detected in clinical samples from patients hospitali...
Article
Metallo-β-lactamase (MBL) producing bacteria constitute nowadays a serious global concern worldwide. The purpose of our present study was to characterize molecular features of MBL producing bacteria and to identify the existing clones in our area. Thirteen MBL-producing-Klebsiella pneumoniae were detected in clinical samples from patients hospitali...
Article
Full-text available
Little is known about non classical HLA molecules in vulvar squamous cell carcinoma (VSCC). Because of the indoleamine‐2,3‐dioxygenase (IDO) immune tolerant role in association with HLA‐G, we evaluated the clinical and prognostic value of HLA‐G, HLA‐E and IDO in VSCC. HLA‐G, HLA‐E and IDO expression was determined by immunohistochemistry in VSCC an...
Article
Full-text available
The aim of our study was to characterize third-generation cephalosporin (3GC)-resistant Enterobacteriaceae isolated over two different periods from patients hospitalized in the Military Hospital of Tunis with special focus to class A β-lactamases. This study included 180 Enterobacteriaceae resistant to 3GC isolated from samples of patients hospital...
Article
Full-text available
Leuconostoc lactis AV1 strain isolated from a Tunisian avocado was characterized as a dextran producer. The promoter P dsrLL and the dsrLL gene encoding the DsrLL dextransucrase responsible for the dextran synthesis were transcriptionally fused to the mCherry coding gene generating the pRCR20 plasmid. Upon plasmid transfer, both AV1n and the dextr...
Article
Full-text available
Swarming is the most rapid surface motility allowing Pseudomonas aeruginosa bacteria to rapidly colonize new surfaces. However, swarming behavior is affected by environmental factors like ultraviolet irradiation (UVc). UVc radiation is the most disinfection technology usually applied for wastewater and proven to be effective to inactivate microorga...
Article
Full-text available
Lactobacilli naturally present in the neonatal gut are believed to be beneficial for the human hosts and are investigated as potential probiotics. In this study, we aimed to characterize six Lactobacillus plantarum strains derived from the feces of a breast-fed infant, for the development of new probiotic cultures. Our attention was focused on L. p...
Article
Full-text available
Tomatoes and tomato based-foods contain beneficial microorganisms and various organic acids that have important nutritional values for human. The objective of this study was to access the physiochemical properties of fermented tomatoes juices and to evaluate the competitiveness of lactic acid bacteria (LAB) against Listeria monocytogenes , Listeria...
Data
The result of Biolog phenotypic microarray analysis data used to support the findings of this study are included within the supplementary information file.
Article
Here, we report the genomic features and the bioremediation potential of Halomonas desertis G11, a new halophilic species which uses crude oil as a carbon and energy source and displays intrinsic resistance to salt stress conditions (optimum growth at 10% NaCl). G11 genome (3.96 Mb) had a mean GC content of 57.82%, 3622 coding sequences, 480 subsys...
Article
Full-text available
Here, we report the genomic features and the bioremediation potential of Halomonas desertis G11, a new halophilic species which uses crude oil as a carbon and energy source and displays intrinsic resistance to salt stress conditions (optimum growth at 10% NaCl). G11 genome (3.96 Mb) had a mean GC content of 57.82%, 3622 coding sequences, 480 subsys...
Article
The production, characterization and potential application in heavy metals and dyes removal of a novel heteropolysaccharide-protein named, gpHb, produced by an haloarchaeal strain Halogeometricum borinquense strain A52 were investigated. The highest gpHb yield of 13.96 ± 0.32 g/L was produced under optimized conditions by response surface methodolo...
Article
Plant associated microorganisms, particularly those exhibiting a plant growth promoting (PGP) effect, play an important role in plant nutrition and health and the adaptation to unfavorable climatic conditions, such as drought which threatens the productivity of agricultural crops. The selection of specific microbial populations in the soil habitats...
Article
New organic/inorganic hybrid (C17H22N2)[CuCl4] was elaborated and studied by single crystal X-ray diffraction. The compound crystallizes in the orthorhombic Pbca space group. The isolated [CuCl4] 2-tetrahedra are slightly distorted building a 0-D anionic framework. The double protonation of the organic cation establish N-H···Cl interactions between...
Article
The synthesis of new substituted arylphosphoramidates is performed in two steps through phosphorylation of the corresponding alcohols followed by aminolysis. The formation of the desired phosphoramidates depends on the subsequent addition of the two alcohols with the amine being added at the last step.The products were obtained in 58–95% yields. Th...
Article
Full-text available
The research and the selection of novel probiotic strains from novel niches are receiving increased attention on their proclaimed health benefits to both humans and animals. This study aimed to evaluate the functional properties of Weissella strains from arid land living-hosts and to select strains with cholesterol-lowering property in vitro and in...
Data
Supplementary Table S1: positive reaction for carbohydrate utilization by two Weissella halotolerans strains using Biolog phenotypic microarray.
Article
Among the various tests commonly used for selecting probiotic microorganisms, the tolerance to gastrointestinal transit conditions remains being commonly used to evaluate the probiotic potential of the strains. Besides, the adhesion to epithelial cells and the competition with pathogens constitute significant traits for evaluating the colonization...