Gyeong Jin Lee

Seoul National University | SNU · Department of Biosystems and Biomaterials Science and Engineering

Cationic barley β-glucan derivatives (bGPEIs) with various polyethylenimine 2k (PEI2k) graft degrees were synthesized by periodate oxidation of backbone vicinal diols and reductive amination of PEI2k for gene delivery systems. bGPEIs could form positively charged (∼40 mV zeta-potential) and nano-sized (∼150 nm) spherical polyplexes. Cytotoxicity of...

Fluorinated intermediate molecular weight polyethylenimine (FP2ks) with various fluorination degrees were synthesized by conjugation with heptafluorobutyric anhydride and the fluorination effect for gene delivery systems was examined. FP2ks could condense pDNA, forming compact, positively charged, and nano‐sized spherical particles. It was thought...

i-motif is cytosine (C)-rich oligonucleotide (ODN) which shows pH-responsive structure change in acidic condition. Therefore, it has been utilized for the trigger of intercalated drug release, responding to environmental pH change. In this study, 2.76 molecules of i-motif binding ODNs (IBOs) were conjugated to each hyaluronic acid (HA) via amide bo...

Background: Phenylboronic acid-polymers (PBA-polymers) have attracted tremendous attention as potential stimuli-responsive materials with applications in drug-delivery depots, scaffolds for tissue engineering, HIV barriers, and biomolecule-detecting/sensing platforms. The unique aspect for PBA-polymers is their interactions with diols, which resul...

We prepared hybrids consisting of Angelica gigas Nakai (AGN) root or flower extract and layered double hydroxide (LDH) for potential anticancer nanomedicine, as decursin species (DS) in AGN are known to have anticancer activity. Dimethylsulfoxide solvent was determined hybridization reaction media, as it has affinity to both AGN and LDH moiety. In...

In order to control Mg/Al ratio in hydrotalcite type layered double hydroxide (LDH), LDH was prepared through coprecipitation under varied synthetic parameters such as initial Mg/Al ratio (from 1 to 20) in metal solution and coprecipitation pH (9.5, 10, 10.5 and 11). Phase evolution of hydrotalcite or impurities was examined with powder X-ray diffr...

Self-assembling multifunctional peptide was designed for gene delivery systems. The multifunctional peptide (MP) consists of cellular penetrating peptide moiety (R 8 ), matrix metalloproteinase-2 (MMP-2) specific sequence (GPLGV), pH-responsive moiety (H 5 ), and hydrophobic moiety (palmitic acid) (CR 8 GPLGVH 5 -Pal). MP was oxidized to form multi...

Agmatine-containing bioreducible polymer, poly(cystaminebisacrylamide-agmatine) (poly(CBA-AG)) was synthesized for gene delivery systems. It could form 200-300 nm sized and positively charged polyplexes with pDNA, which could release pDNA in reducing environment due to the internal disulfide bonds cleavage. poly(CBA-AG) also showed a spontaneous de...

Crosslinked bioreducible polypropylenimine-cystaminebisacrylamides (PPI-CBAs) were synthesized for gene delivery systems. They formed nano-sized polyplexes with high stability even in reducing condition probably due to the re-crosslinking. PPI-CBAs displayed high transfection efficiency comparable to PEI25k in serum condition. However, cytotoxicity...

Objective: Layered double hydroxide (LDH) nanoparticles have been studied as cellular delivery carriers for anionic anticancer agents. As MTX and 5-FU are clinically utilized anticancer drugs in combination therapy, we aimed to enhance the therapeutic performance with the help of LDH nanoparticles. Method: Anticancer drugs, MTX and 5-FU, and the...