Gwenola Gouesbet

University of Rennes | UR1 · University of Rennes, CNRS, ECOBIO [(Ecosystèmes, biodiversité, évolution)] - UMR 6553, Rennes, France

The lesser mealworm, Alphitobius diaperinus , is an invasive tenebrionid beetle and a vector of pathogens. Due to the emergence of insecticide resistance and consequent outbreaks that generate significant phytosanitary and energy costs for poultry farmers, it has become a major insect pest worldwide. To better understand the molecular mechanisms be...

Plant functioning and responses to abiotic stresses largely involve regulations at the transcriptomic level via complex interactions of signal molecules, signaling cascades, and regulators. Nevertheless, all the signaling networks involved in responses to abiotic stresses have not yet been fully established. The in-depth analysis of transcriptomes...

The identification and characterization of bona fide abiotic stress signaling proteins can occur at different levels of the complete in vivo signaling cascade or network. Knowledge of a particular abiotic stress signaling protein could theoretically lead to the characterization of complete networks through the analysis of unknown proteins that inte...

Herbicides and their degradation products contribute to soil pollution and to its impact on soil biodiversity and functioning. Soil herbicide pollution presents characteristics of global planetary threats, with harmful consequences for soil ecology, for ecosystem functions and services mediated by natural plant communities and for crop production s...

Ecological interactions are rarely taken into account in environmental risk assessment. The objective of this work was to assess how interspecific competition affects the way plant species react to herbicides and more specifically how it modifies the concentration-response curves that can be built using ecotoxicological bioassays. To do this, we re...

HIGHLIGHTS • Characterization of soil pesticide contamination requires multi-level studies. • Plant-pesticide dynamics was studied at field scale in vegetative filter strips. • Pesticide persistence in soils was interannual, multi-source and multi-compound. • Pesticide persistence and plant dynamics showed case-by-case relationships. • Epoxiconazol...

Species Sensitivity Distributions (SSD) are widely used in environmental risk assessment to predict the concentration of a contaminant that is hazardous for 5% of species (HC5). They are based on monospecific bioassays conducted in the laboratory and thus do not directly take into account ecological interactions. This point, among others, is accoun...

Soil pollution by anthropogenic chemicals is a major concern for sustainability of crop production and of ecosystem functions mediated by natural plant biodiversity. The complex effects on plants are however difficult to apprehend. Plant communities of field margins, vegetative filter strips or rotational fallows are confronted with agricultural po...

The extent of residual contaminations of pesticides through drift, run-off and leaching is a potential threat to non-target plant communities. Arabidopsis thaliana responds to low doses of the herbicide atrazine, and of its degradation products, desethylatrazine and hydroxyatrazine, not only in the long term, but also under conditions of short-term...

Plant hormone signaling plays an important role in many physiological and developmental processes including stress response. With the advent of new post-genomic molecular techniques the potential for increasing our understanding of the impact of hormone signaling on gene expression and adaptive processes has never been higher. Unlocking the molecul...

Herbicides are pollutants of great concern due to environmental ubiquity resulting from extensive use in modern agriculture and persistence in soil and water. Studies at various spatial scales have also highlighted frequent occurrences of major herbicide breakdown products in the environment. Analysis of plant behavior toward such molecules and the...

Implications of field margin plant communities in the environmental dynamics of pesticides pollutions. Several types of regulations, at the European and national levels, advocate setting up vegetative buffer strips in order to control or prevent the contamination of ecosystems by agricultural pollutions. The specific implication of the plant commun...

Summary - Implications of field margin plant communities in the environmental dynamics of pesticides pollutions. Several types of regulations, at the European and national levels, advocate setting up vegetative buffer strips in order to control or prevent the contamination of ecosystems by agricultural pollutions. The specific implication of the pl...

Herbicide impact is usually assessed as the result of a unilinear mode of action on a specific biochemical target with a typical dose-response dynamics. Recent developments in plant molecular signaling and crosstalk between nutritional, hormonal and environmental stress cues are however revealing a more complex picture of inclusive toxicity. Herbic...

Forthcoming seminar Tuesday 24th 2016, 11:10, Room R0-A, Cité des Congrès de Nantes, http://www.lacite-nantes.com/uk/index.html Pesticides are pollutants of high concern due to environmental ubiquity resulting from extensive use in modern agriculture and persistence in soil and water. Study of plant behaviour toward such molecules and their breakdo...

Lolium perenne, which is a major component of pastures, lawns, and grass strips, can be exposed to xenobiotic stresses due to diffuse and residual contaminations of soil. L. perenne was recently shown to undergo metabolic adjustments in response to sub-toxic levels of xenobiotics. To gain insight in such chemical stress responses, a de novo transcr...

Au sein des paysages agricoles, la mise en place de bandes enherbées (BE) a pour objectif de limiter la contamination des milieux en aval des parcelles cultivées, tels que les cours d’eau. Ces dispositifs végétalisés sont des zones tampons qui interceptent les flux d’engrais et de produits phytosanitaires (pesticides). Au sein de la Zone Atelier Ar...

There is currently a major challenge to improve photosynthetic yield for increased sustainable crop production and meeting mankind's nutritional requirements. Among other factors, suboptimal carbon partitioning and suboptimal photoprotection may contribute to limitations of photosynthetic efficiency (1). Herbicide and oxidative stress tolerance can...

SEE YOU AT THE CONFERENCE ! POSTER 454 ABIOTIC STRESS SESSIONS CONTACT: Diana Alberto, diana.alberto@univ-rennes1.fr

Herbicide and oxidative stress tolerance can involve mechanisms that are related to photosynthesis efficiency and carbon dynamics. In this context, an Arabidopsis thaliana T-DNA insertional mutant was identified and characterized for enhanced tolerance to the singlet-oxygen-generating herbicide atrazine in comparison to wild-type.This enhanced atra...

Plant communities are confronted with a great variety of environmental chemical stresses. Characterization of chemical stress in higher plants has often been focused on single or closely related stressors under acute exposure, or restricted to a selective number of molecular targets. In order to understand plant functioning under chemical stress co...

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Proposition d’une nouvelle approche consistant à construire des serres de phytoremédiation directement sur les sites pollués pour éliminer les nombreux blocages à la phytoremédiation en conditions réelles de terrain.

ABSTRACT: Transfer of plant-driven soil depollution (phytoremediation) processes from controlled conditions (in vitro, growth chambers, greenhouses) to complex conditions in the field is often reported to be hampered by environmental factors, such as climatic conditions, soil properties, abiotic stress, or multipollution combinations (polycyclic ar...

Selected Conference : Anne-Antonella Serra Wednesday 14th May 11:50 Montreal Session Room Congress Center Basel

Agricultural activities use large amounts of pesticides in order to increase crop productivity. However, small proportions of applied pesticides reach intended targets, whereas the majority is dispersed in the environment, thus affecting soil and water quality. In order to reduce pesticide runoffs and improve water quality in agricultural landscape...

Anthropic changes and chemical pollution confront wild plant communities with xenobiotic combinations of bioactive molecules, degradation products, and adjuvants that constitute chemical challenges potentially affecting plant growth and fitness. Such complex challenges involving residual contamination and mixtures of pollutants are difficult to ass...

Catabolic processes providing alternative sources of electron for the mitochondrial electron transport chain are progressively emerging as important players in plants for stress responses, nutritional switch responses and growth and development. In this context, xenobiotics, such as the herbicide atrazine, which are at the crossroads of xenobiotic...

Higher plants are exposed to natural environ-mental organic chemicals, associated with plant–environ-ment interactions, and xenobiotic environmental organic chemicals, associated with anthropogenic activities. The effects of these chemicals result not only from interaction with metabolic targets, but also from interaction with the complex regulator...

Higher plants are exposed to natural environmental organic chemicals, associated with plant-environment interactions, and xenobiotic environmental organic chemicals, associated with anthropogenic activities. The effects of these chemicals result not only from interaction with metabolic targets, but also from interaction with the complex regulatory...

A method is provided for phytoremediating a site polluted by at least one type of pollutant, comprising at least one step comprising growing, on this polluted soil, at least one phytoremediating plant capable of fixing at least a portion of this pollutant, and comprising a subsequent step comprising of harvesting or destroying this plant that has f...

Anthropogenic changes and chemical pollution confront plant communities with various xenobiotic compounds or combinations of xenobiotics, involving chemical structures that are at least partially novel for plant species. Plant responses to chemical challenges and stimuli are usually characterized by the approaches of toxicology, ecotoxicology, and...

Localization of T-DNA insertion in eat1 mutant line by Southern blot analysis. Five µg of genomic DNA from eat1 mutant were separately digested with HindIII and NdeI and resulting DNA fragments were separated by agarose gel electrophoresis and then blotted onto a nylon membrane. Hybridization was carried out with specific DIG-labelled probes corres...

Enhanced biomass phenotype of BH755830 Arabidopsis mutant line. Root and shoot fresh weights are given. Seeds of the BH755830 Arabidopsis mutant line were germinated on 1x MS-agar medium in the absence of atrazine, and plantlet development was carried out for 15 days. Values are the mean (± S.E.M.) of measurements on at least sixteen 15-day-old pla...

An Arabidopsis thaliana T-DNA insertional mutant was identified and characterized for enhanced tolerance to the singlet-oxygen-generating herbicide atrazine in comparison to wild-type. This enhanced atrazine tolerance mutant was shown to be affected in the promoter structure and in the regulation of expression of the APL4 isoform of ADP-glucose pyr...

The dynamics of plankton microorganisms is dependent on physico-chemical constraints which must be perceived by individual cells and integrated into physiological responses that ensure survival and growth control. These cellular adjustments have a strong impact on molecular fluxes of nutrients through the community and on the interactions between p...

Ecology Letters (2010) 13: 776–791 Environmental genomics and genome-wide expression approaches deal with large-scale sequence-based information obtained from environmental samples, at organismal, population or community levels. To date, environmental genomics, transcriptomics and proteomics are arguably the most powerful approaches to discover com...

Sugars, products of photosynthesis, have pleiotropic effects in plants. Besides their function as metabolic resources essential for energy supplies, carbon storage, biosynthesis pathways and cell wall formation, they also play pivotal role as signalling molecules. The complexity of eukaryotic organisms and the presence of interferences between sign...

Ecology Letters (2010) 13: 776–791AbstractEnvironmental genomics and genome-wide expression approaches deal with large-scale sequence-based information obtained from environmental samples, at organismal, population or community levels. To date, environmental genomics, transcriptomics and proteomics are arguably the most powerful approaches to disco...

Human activities have generated large-scale pollutions of ecosystems, where plants are directly and massively affected, thus leading to modifications of population or community dynamics. Despite a large diversity of structures and primary targets, many classes of pollutants act on plants through effects on Reactive Oxygen Species (ROS) levels, oxid...

Soluble sugars are involved in responses to stress, and act as signalling molecules that activate specific or hormone cross-talk transduction pathways. Thus, exogenous sucrose treatment efficiently induces tolerance to the herbicide atrazine in Arabidopsis thaliana plantlets, at least partially through large-scale modifications of expression of str...

Detections and quantification have been done on 3-week-old MS-grown Arabidopsis thaliana plantlets subjected to subsequent treatment (12, 24, 48 or 72 hours) with 80 mM mannitol (M), 80 mM sucrose (S), 80 mM mannitol plus 10 μM atrazine (MA) or 80 mM sucrose plus 10 μM atrazine (SA). Hydrogen peroxide content was expressed as μ moles of H2O2 per g...

Singlet oxygen detections using the SOSG probe have been done on 3-week-old MS-grown Arabidopsis thaliana plantlets subjected to subsequent treatment (12, 24, 48 or 72 hours) with 80 mM mannitol (M), 80 mM sucrose (S), 80 mM mannitol plus 10 μM atrazine (MA) or 80 mM sucrose plus 10 μM atrazine (SA). Image analysis and quantification of fluorescenc...

Detections and quantification have been done on 3-week-old MS-grown Arabidopsis thaliana plantlets subjected to subsequent treatment (12, 24, 48 or 72 hours) with 80 mM mannitol (M), 80 mM sucrose (S), 80 mM mannitol plus 10 μM atrazine (MA) or 80 mM sucrose plus 10 μM atrazine (SA). Superoxide radical content was expressed as nmoles of reduced NBT...

Besides being essential for plant structure and metabolism, soluble carbohydrates play important roles in stress responses. Sucrose has been shown to confer to Arabidopsis seedlings a high level of tolerance to the herbicide atrazine, which causes reactive oxygen species (ROS) production and oxidative stress. The effects of atrazine and of exogenou...

Erwinia chrysanthemi insertion mutants were isolated that grew poorly specifically in the presence of glycine betaine (GB) or its analogues in high-salt media. Transposon insertions were found to affect the bspA gene, which forms an operon including the psd locus coding for phosphatidylserine decarboxylase. Initial GB uptake is not affected by the...

L’implication des sucres solubles dans la tolérance des plantules d’Arabidopsis thaliana à l’herbicide atrazine, et au stress oxydatif généré, a été étudiée par une approche transcriptomique. Cette analyse a révélé que la protection induite par le saccharose face aux dommages causés par l’atrazine est associée à d’importantes modifications d’expres...

Different classes of herbicide act on plants through direct induction of reactive oxygen species (ROS) production and oxidative injury. Thus, herbicides of the triazine, phenolic and urea families, which bind to the D1 protein, inhibit photosystem II (PSII), and block electron transfer to the plastoquinone pool, thus causing the production of tripl...

Physiological effects of atrazine and sucrose treatments. Arabidopsis plantlets were grown on Murashige and Skoog agar medium and transferred at the 1.02 development stage [20] to Murashige and Skoog agar medium supplemented with mannitol (80 mM), mannitol (80 mM) plus atrazine (10 μM), sucrose (80 mM) and sucrose (80 mM) plus atrazine (10 μM). Pic...

Effects of atrazine and sucrose treatments on chlorophyll content. Arabidopsis plantlets transferred to Murashige and Skoog agar medium supplemented with mannitol (80 mM)(A), sucrose (80 mM)(B), mannitol (80 mM) plus atrazine (10 μM)(C), and sucrose (80 mM) plus atrazine (10 μM)(D) were harvested after 0, 1, 4 and 8 days of treatment for pigment de...