Guy Daculsi

French Institute of Health and Medical Research | Inserm · Laboratory of Osteoarticular and Dental Tissue Engineering

Biphasic calcium phosphate (BCP) bioceramics (hydroxyapatite/tricalcium phosphate, or HA/TCP) for tissue engineering and drug delivery systems is a unique know-how. A mechanical mixture of HA and TCP does not lead to such bioactive ceramics. The wet elaboration conditions of calcium-deficient apatite (CDA) or CDHA, followed by sintering, converts i...

Due to their osteoconductive and inductive properties, a variety of calcium phosphate (CaP) scaffolds are commonly used in orthopaedics as graft material to heal bone defects. In this study, we have used two CaP scaffolds with different hydroxyapatite (HA) and β‐tricalcium phosphate (β‐TCP) ratios (MBCP®; 60/40 and MBCP⁺®; 20/80), to investigate th...

We report the novel use of a tuneable, non-integrating viral gene delivery system to bone that can be combined with clinically approved biomaterials in an 'off-the shelf' manner. Specifically, a doxycycline inducible Tet-on adenoviral vector (AdTetBMP-2) in combination with mesenchymal stromal cells (MSCs), fibrin and a biphasic calcium phosphate c...

Tympanoplasty is the reference for cholesteatoma treatment in ear, nose, and throat (ENT surgery), with the realization of a closing technique, which preserves the external auditory canal. An insufficient pneumatisation is an element favoring the occurrence and recurrence of cholesteatoma. In a prospective clinical pilot study, we have realized the...

Bioceramics draw attention in bone tissue engineering field since their biomimetic properties regarding bone attribute. In this context, a concept of smart bioceramics granules made of Hydroxyapatite have been set up, enhancing surface area available to body fluids containing proteins and cell adhesion for bone forming respectively thanks to microp...

Bioceramics is a relatively new field; it did not exist until the beginning of 1970, when these materials were shown to restore osteoarticular and dental functions, as well as act as a replacement material for autografts and allograft bone reconstructions. Bioceramics used to replace, repair, or reconstruct human body parts or complex living tissue...

Bioceramics is a relatively new field; it did not exist until the beginning of 1970, when these materials were shown to restore osteoarticular and dental functions, as well as act as a replacement material for autografts and allograft bone reconstructions. Bioceramics used to replace, repair, or reconstruct human body parts or complex living tissue...

There is a large variety of commercial bioceramic bone substitutes; however, the prerequisites for bone reconstruction and tissue engineering are often absent in research and clinical applications. The main criteria for the use of bioceramics are easily handled biomaterials that are solid, injectable, and/or shapeable. Furthermore, the material mus...

There are numerous clinical indications for bone grafts. The ideal graft material should favor bone apposition and growth while simultaneously being degraded by body fluids and cells. Ultimately, the material should be replaced by mature bone tissue within a healing period of weeks. Because autologous and allogenic bone grafts fulfill some of these...

The commercial offer for bioceramic bone substitutes is very large, however, the prerequisites for applications in bone reconstruction and tissue engineering, are most often absent. The main criteria being: on the one hand physico-chemical features providing surgeons with an injectable and/or shapeable biomaterial; on the second hand the multi-scal...

The present study focuses on the physico-chemical and structural properties of composite scaffolds composed of biopolymer matrices (collagen or polysaccharide) loaded with calcium phosphate granules. A systematic three-dimensional analysis method was used to quantitatively characterize a series of plugs, strips and putties in terms of percentage of...

Bioceramics is a relatively new field; it did not exist until the beginning of 1970, when these materials were shown to restore osteoarticular and dental functions, as well as act as a replacement material for autografts and allograft bone reconstructions. Since the turn of the millennium, numerous synthetic bone graft materials have become availab...

There is a large variety of commercial bioceramic bone substitutes; however, the prerequisites for bone reconstruction and tissue engineering are often absent in research and clinical applications. The main criteria for the use of bioceramics are easily handled biomaterials that are solid, injectable, and/or shapeable. Furthermore, the material mus...

Solid-state transformation of CDA at high temperature has been investigated using TEM microscopy and diffraction from sintered biphasic calcium phosphate (hydroxyapatite-HA, and beta-tricalcium phosphate-TCP). Microcrystals, between 200nm and 800nm approximately, separated by grain boundaries were found to be either HA-HA or TCP-TCP, but not HA-TCP...

Bioceramics combined with isolated stem cells, or with total bone marrow, constitute the main strategies under consideration in the field of bone tissue engineering. In the present preclinical study, two biphasic calcium phosphate scaffolds currently on the market, MBCP® and MBCP+®, with different hydroxyapatite/β-tricalcium phosphate ratio, were i...

Various bioceramics or xenograft has been used to avoid autograft. However, there are large differences in the chemistry, the micro- and macrostructure, and consequently the performance in terms of resorption, absorption, and regeneration of physiological bone. The differences in such available bioceramics were reported and critical data presented....

Conclusion: The TricOs™/MBCP(®) and fibrin sealant composite was a convenient, effective, and well-tolerated material for mastoid cavity filling and immediate reconstruction of the external auditory meatus after cholesteatoma surgery with canal wall down (CWD). Objective: To assess the tolerance and osteointegration of a bone graft substitute, T...

Resorbable synthetic bone graft materials are mainly calcium phosphates. These materials differ in chemical composition and physical properties, particularly in regards of osteoconduction, osteogenic and/or osteoinductive properties. Several scaffolds are characterized and compared. Results from preclinical and clinical studies are selected. Osteoc...

It is often necessary to restore bone structure following the loss of osseous substance. Therefore, the use of bone substitutes made of synthetic calcium phosphate (CaP) ceramics has become more widespread. Granular forms of these biomaterials have demonstrated osteogenic capacities within various sites. Recently, a combination of CaP microporous g...

Heat-induced osteonecrosis represents a simple, rapid, and inexpensive method for reproducing the effects of bone disease. In the present study, we employed this technique to induce osteonecrosis in femoral defects in rabbits and assessed the efficacy of treatment using Biphasic Calcium Phosphate (BCP) granules (MBCP+™, Biomatlante SA). After 3 wee...

The association of Micro Macroporous Biphasic Calcium Phosphate granules and t fibrin sealant has been developed to answer a challenging request of orthopaedic surgeons: a biocompatible, osteogenic, mouldable, and self-hardening bone substitute able to fill bone defects. The aim of this study was the evaluation of the performance and safety of the...

Physico-chemical characteristics impact directly or indirectly the bioactive properties of biomaterials, it is then essential to correlate it with their effect in vivo. A panel of biomaterials available on the market, based on Hydroxyapatite (HA) and Tricalcium phosphate (β-TCP) is studied in terms of surface area, hydrophilicity, porosity, zeta po...

Restoring alveolar bone following tooth extraction or pathological diseases is important, and recent efforts have been made to overcome the use of autografts during dental implantation. Although micro-macroporous biphasic calcium phosphate (MBCPTM) has performed well in orthopedic procedures, few studies have investigated its use in dentistry. Here...

The invention relates to the use of a silanised hydroxypropylmethylcellulose (HPMC) or silanised hydroxyethylcellulose (HEC) hydrogel, self-crosslinking as a function of pH, for the three-dimensional ex vivo culture of chondrocytes.

Introduction: Intra-articular distal radial fractures in young subjects occur in severe trauma. Articular reduction needs to be anatomical. We report four cases with the particularity of having a 90° or 180° rotated lunate fossa. Our goal is to bring out the positive aspects of surgical procedure by volar medial approach and to assess long-term fu...

INTRODUCTION: Intra-articular distal radial fractures in young subjects occur in severe trauma. Articular reduction needs to be anatomical. We report four cases with the particularity of having a 90° or 180° rotated lunate fossa. Our goal is to bring out the positive aspects of surgical procedure by volar medial approach and to assess long-term fun...

Calcium phosphate bioceramic granules associated with hydrosoluble polymers formed putties currently more used in clinical applications as they are easy to handle (injectability, moldability). In this study, 2 kinds of materials were tested in rabbit bone defects. The first one is In'Oss (TM) (Biomatlante), a microporous biphasic CaP granules (BCP,...

The main goal of this study was to succeed in the relevant association of well-known osteoconductive biphasic calcium phosphate (BCP) made of Hydroxyapatite (20% HA) and β-Tricalcium Phosphate (80% β-TCP) crystallographic phases and resorbable poly (L-lactide-co-D,L-lactide)(PLDLLA) 3D matrices synthesized by electrospinning. Two types of mineral p...

The development of CaP ceramics involved a better control of the process of resorption and bone substitution. Micro Macroporous Biphasic CaP, (MBCP+) is a concept based on an optimum balance of the more stable phase of HA and more soluble TCP. The material is soluble and gradually dissolves in the body, seeding new bone formation as it releases Ca...

The objective of the study was to compare clinical efficiency of the fusion after reconstruction with an anatomically shaped PEEK cage associated with a iliac crest autograft or MBCP in the treatment of cervical disc disease in randomized clinical trial. A multicente randomized, comparative and prospective study on 58 patients, with a 12 months fol...

The objective of this study deals with preclinical and clinical cases of absorbable composite interference screws of next generation in osteoarticular surgery. These implants are made of resorbable polymers PLA either amorphous or semi-cristalline, associated with granules of microporous biphasic calcium phosphate ceramic. A preclinical study was p...

This study reports several clinical cases for orthopaedic bone regeneration using an injectable hone substitute (MBCP Gel (R)) to demonstrate its safe use and efficiency in clinical applications. The biomaterial is a composite of microporous bioceramic hydroxyapatite granules that are associated with beta tricalcium phosphate (MBCP) and a synthetic...

The osseointegration around titanium mini-implants installed in macroporous biphasic calcium phosphate (MBCP) blocks was evaluated after incubation with recombinant human bone morphogenetic protein-2 (rhBMP-2) in an ectopic subcutaneous rat model. Mini-implants (φ1.8×12 mm) were installed in MBCP blocks (bMBCPs, 4×5×15 mm) loaded with rhBMP-2 at 0....

【Abstract】The authors reported the case of a 27-year-old man who sustained an irreducible postero-lateral traumatic dislocation of the hip with capsular and labral entrapment. Initial X-rays showed only a small acetabular fragment. After two attempts to reduce the hip with muscle paralysis under general anaesthesia failed, the patient was treated b...

It was demonstrated that microstructured surfaces improve cell spreading and bone ingrowth. Particularly, the surface roughness modulates the osseointegration of orthopaedic and dental implants. We have developed an innovative grit blasting process using Biphasic Calcium Phosphate, a Resorbable Biocompatible Blast Media (RBBM). PEEK is biocompatibl...

A new biphasic calcium phosphate ceramic material Hydros™ has been developed. The main attractive feature of BCP ceramic is their ability to form a strong direct bond with the host bone resulting in a strong interface. Currently, granules are more and more used in moldable, injectable bone substitutes. However, the biological behaviour of the parti...

Due to the lack of macroporosity in current available Calcium Phosphate cement used in osteoarticular surgery, Micro and Macroporous Biphasic CaP Cement (MCPC™) was developed. The MCPC™ concept was the association of a settable and a fast resorbable matrix and a sieved fraction of microporous biphasic calcium phosphate (BCP) granules, recognized fo...

Bone defects resulting from tumour resection or curettage are most commonly reconstructed with autologous bone graft which is associated with limited availability and donor site morbidity. Recent research has focussed on synthetic biomaterials as bone graft substitutes. The aim of this study was to assess the safety and efficiency of a bone substit...

Although autologous or allogenous bone grafting materials are widely used to repair skeletal tissue, their limitations induce surgeons to look for more advanced therapies such as tissue engineering. However, bone tissue engineering still lacks proofs of clinical efficacy, possibly due to the inadequate design of the tissue-engineered constructs in...

The dental system and teeth morphology of Anarhichas lupus L. are perfectly suited to its mode of feeding. The anterior teeth are used for grasping preys. The molariform lateral and posterior teeth, palatine and vomer, are used for crushing ingested organisms with hard shells such as gasteropods and lamellibranchs. Microradiography gives evidence o...

The objective of this study was to determine the effect of calcium phosphate mineral content on the bone in-growth at the expense of composite of co-polylactide polymer charged with 2 different ratios of β-TCP granules (10 and 24 w-% of β-TCP). The evaluation was realized in a long term rabbit bone model. After 24, 48 and 76 weeks, the implants wer...

Calcium phosphate granules associated with hydrogels were developed as bone substitutes for various clinical applications. These Injectable Bone Substitutes (IBS) should regenerate bone tissue. The efficiency of these multiphasic materials was due to the osteogenic properties of the Microporous Biphasic Calcium phosphate (BCP) granules. The hydroso...

To reconstruct segmental mandibulectomy using calcium phosphate ceramics and collagen membrane with a delayed bone marrow grafting in experimental animals. Defects of segmental mandibulectomy were filled with calcium phosphate granules and wrapped with a collagen membrane in 4 dogs and left empty as a control in 2 dogs. Two months later, a bone mar...

We aimed to quantify bone colonization toward an untreated titanium implant with primary stability following filling of the defect with micromacroporous biphasic calcium phosphate (MBCP) granules (TricOs) or MBCP granules mixed with fibrin sealant (Tisseel). Medial arthrotomy was performed on the knees of 20 sheep to create a bone defect (16 mm dee...

Reconstructing irradiated mandibles with biomaterials is still a challenge but little investigated. We collected data that could help us understand studies in the field of regeneration with biomaterials and irradiated bone. Systematic review of the literature. Delay and duration of radiation delivery and total equivalent dose are the most variable...

We developed 20 years ago, biphasic calcium phosphate ceramics (BCP). The BCP concept is determined by a balance of the more stable HA phase and more soluble TCP. BCP is gradually resorbed, seeding new bone formation. The main attractive feature of BCP is the ability to form a direct bone bonding resulting in a strong interface. This interface is t...

Current bone tissue engineering models would not be recommended for maxillofacial oncological defects because an immediate post implantation irradiation would decrease their osteoinductive potential. The aim of this study was to reconstruct bone defects applying the concept of a bone marrow graft after the initial reconstruction and radiotherapy. S...

Different resorbable apatitic calcium phosphate bone cements are currently used. They present good mechanical properties and reasonable setting times. However they remain dense and do not provide rapid bone substitution because of the lack of macroporosity. The present study compared 3 kinds of CaP cements presenting different crystallinity charact...

Understanding of interactions between cells and biomaterials is a huge parameter for improving tissue engineering and regenerative medical fields. Many different materials have already been tested (including calcium phosphate ceramics) and it has been established that surface characteristic is a parameter that influences cell responses. The aim of...

Oral carcinomas are frequently treated with a nonsegmental mandibulectomy plus radiotherapy. Improving the quality of life of these patients depends on the possibilities for dental rehabilitation. The aim of this study was to increase the alveolar ridge height. Twelve white New Zealand rabbits underwent surgery to produce a 11 x 9 x 7 mm(3) horizon...

Macroporous beta tricalcium phosphate (beta-TCP) scaffolds were evaluated as potential carriers and delivery systems for bone morphogenetic protein-2 (BMP-2). Chemical etching was performed to increase the available surface and thus the protein loading. X-ray diffraction and infrared spectrocopy analyses confirmed the preparation of pure beta-TCP s...

Calcium phosphate bioceramic granules associated with hydrosoluble polymers were developed as bone substitutes for various maxillofacial and orthopaedic applications. These injectable bone substitutes, support and regenerate bone tissue and resorb after implantation. The efficiency of these multiphasic materials is due to the osteogenic and osteoco...

The aim of this study was to assess the efficacy and safety of macroporous biphasic calcium phosphate (MBCP())/fibrin grafts (TricOs((R)))/(Tisseel((R))) for sinus lift augmentation in sheep. Autologous bone grafts were used as a positive control, and dental implants were placed to assess the efficiency of the composite. A total of 12 adult sheep (...

Hydroxyapatite and related calcium phosphates (CaPs) are similar in composition to the mineral part of bones and teeth. Synthetic CaPs are successfully used for filling bone defects in various clinical indications as they are considered bioactive and osteoconductive, guiding the bone healing process. Nevertheless, most of the synthetic bone substit...

Mandibular reconstruction in head and neck oncology uses a number of techniques whose results are inconsistent and whose indications do not benefit from consensus. A review of the literature allowed us to assemble the available knowledge on current mandibular reconstruction techniques, their functional results, and the research perspectives. Margin...

The purpose of this study was to assess the effects of high doses of ionising radiation on the histology and healing of bone in an experimental model of 12 inbred rats. Ten of the rats had external irradiation of a single dose of 30 or 45 Gy on the hind limbs, which is equivalent to 2 or 3 times the routine doses used for treatment in humans. Three...

The bone tissue engineering models used today are still a long way from any oncologic application as immediate postimplantation irradiation would decrease their osteoinductive potential. The aim of this study was to reconstruct a segmental critical size defect in a weight-bearing bone irradiated after implantation. Six white New Zealand rabbits wer...

We report clinical, radiological and histological findings following high tibial valgisation osteotomy (HTVO) using micro-macroporous biphasic calcium phosphate wedges fixed with a plate and locking screws. From 1999 to 2002, 43 knees were operated on and studied prospectively. All underwent clinical and radiological follow-up at days 1, 90, and 36...

A new biphasic calcium phosphate ceramic material has been developed in our laboratory. It is composed of 60% of hydroxyapatite and 40% of beta-tricalcium phosphate, based on three granulometries (submicron, round microporous 80-200 mum and macro microporous 0.5-1 mm particles) and hydrated with water leading the formation of a putty filler for bon...

The aim of this study was to evaluate the capacity of the association of calcium phosphate cement with collagen membrane for large segmental bone defect. Six adult beagle dog underwent defect creation in the ulna and was reconstructed with combination of macroporous calcium phosphate cement MCPC® and resorbable collagen membrane EZ Cure®. After 6 m...

We have developed a new injectable bone substitute combining specific granules of BCP with or without radiopaque elements with a reversible thermo sensitive resorbable carrier such as Pluronic F-127. The composite is liquid at ambient temperature and set as hydrogel at 37°C. Rabbit experiment demonstrates high biocompatibility and bone ingrowth at...

An hydrated putty was prepared by mixing submicron particles, rounded particles and granules of Biphasic Calcium Phosphate (BCP) ceramics composed of HA and β-TCP phases. The material filled entirely critical sized defects in the femoral epiphysis of NZW rabbits. After 3, 6 and 12 weeks, histology revealed that submicron particles were rapidly degr...

Current resorbable membranes are generally from animal origin, like collagen, and are largely used in dentistry or orthopaedic for TGR (Tissue Guided Regeneration), in neurosurgery for Dura matter healing and others. Calcium alginates are generally used for wound dressing (external uses). The purpose of this study was to evaluate the osteogenic pro...

We have developed a novel macroporous calcium phosphate cement MCPC® that sets to poorly crystalline apatite after mixing the powder component with an aqueous solution and has interconnective macroporosity We performed cranioplasty on rat model by injecting the new macroporous calcium phosphate cement MCPC®. The mechanical property of the cement is...

The purpose of this study was to compare bone reconstruction using either mesenchymal stem cells (MSCs) or total bone marrow (TBM) in association with biphasic calcium phosphate (BCP) granules after irradiation in a rat model. Three weeks after an external irradiation of the hind limbs of rats, four bone defects were created per animal. The defects...

Endodontic treatment in dentistry is a delicate procedure and many treatment attempts fail. Despite constant development of new root canal filling techniques, the clinician is confronted with both a complex root canal system and the use of filling materials that are harmful for periapical tissues. This paper evaluates reported studies on biomateria...

Publisher Summary This chapter presents that the Biphasic Calcium Phosphate (BCP) concept based on the intimate mixture of Hydroxyapatite (HA) and β-Tricalcium Phosphate (β-TCP) is founded on: (1) partial dissolution of the Calcium Phosphate (CaP) ceramic macro crystals (principally, β-TCP) causing an increase in the calcium and phosphate concentra...

Some biomaterials are able to induce ectopic bone formation in muscles of large animals. The osteoinductive potential of macro- micro-porous biphasic calcium phosphate (MBCP) ceramic granules with fibrin glue was evaluated by intramuscular implantation for 6 months in six adult female sheep. The MBCP granules were 1-2 mm in size and were composed o...

The aim of this study was to study bone marrow quality from various location and species for reconstruction of segmental critical size defect in irradiated weigh bearing bone. Sample of bone marrow aspirates from rabbits and Beagle dog were analyzed. Rabbits were implanted with a composite associating resorbable collagen membrane plus micro macropo...