Gustavo Monnerat

Gustavo Monnerat
Instituto Nacional De Cardiologia · Biomarkers

PhD

About

57
Publications
9,504
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462
Citations
Introduction
I have a PhD in Human Physiology and deep interest in metabolism, cardiovascular diseases and body weight regulation. In my research, I apply mass spectrometry and molecular strategies for metabolites and proteins analysis
Additional affiliations
April 2014 - present
Federal University of Rio de Janeiro
Position
  • PostDoc Position
September 2013 - April 2014
University of Bonn
Position
  • PhD Student
Description
  • Visiting PhD Student
Education
September 2013 - April 2014
University of Bonn
Field of study
  • Physiology
January 2011 - April 2014
Federal University of Rio de Janeiro
Field of study
  • Physiology

Publications

Publications (57)
Preprint
Full-text available
Testosterone is a hormone that plays a key role in carbohydrate, fat, and protein metabolism. Testosterone deficiency is associated with multiple comorbidities, e.g ., metabolic syndrome and type 2 diabetes. Despite its importance in many metabolic pathways, the mechanisms by which it controls metabolism are not fully understood. The present study...
Article
During fetal development, cardiomyocytes switch from glycolysis to oxidative metabolism to sustain the energy requirements of functional cells. State-of-the-art cardiac differentiation protocols yield phenotypically immature cardiomyocytes, and common methods to improve metabolic maturation require multistep protocols to induce maturation only afte...
Article
Full-text available
IntroductionAlthough sarcopenia greatly affects health and quality of life in older people, its pathophysiological causes are not fully elucidated. To face this challenge, omics technologies can be used. The metabolome gives a vision of the interaction between the genome and the environment through metabolic networks, thus contributing in clarifyin...
Article
New robust and reproducible differentiation approaches are needed to generate induced pluripotent stem cell (iPSC)-derived cardiomyocytes of specific subtypes in predictable quantities for tissue-specific disease modeling, tissue engineering, and eventual clinical translation. Here, we assessed whether powdered decellularized extracellular matrix (...
Preprint
Full-text available
Malignant melanoma (MM) develops from the melanocytes and in its advanced stage is the most aggressive type of skin cancer. Here we report a comprehensive analysis on a prospective cohort study, including non-tumor, primary and metastasis tissues (n=77) with the corresponding plasma samples (n=56) from patients with malignant melanoma. The tumors a...
Conference Paper
Extracellular matrix (ECM) can directly modulate cell proliferation, migration and differentiation by mediating diverse growth factors and signaling interactions. Protocols for cardiomyocyte differentiation of induced pluripotent stem cells (iPSCs) that recapitulate cardiac development frequently result in a mixed cardiac cell population dominated...
Article
Full-text available
Decellularized cardiac extracellular matrix scaffolds with preserved composition and architecture can be used in tissue engineering to reproduce the complex cardiac extracellular matrix. However, evaluating the extent of cardiomyocyte repopulation of decellularized cardiac extracellular matrix scaffolds after recellularization attempts is challengi...
Article
Full-text available
Humoral factors released during ischemic preconditioning (IPC) protect the myocardium against ischemia/reperfusion (I/R) injury. We have recently identified 10 kDa-heat shock protein (HSP10) and a fraction of small 5-10 kDa peptides (5-10-sP) in the coronary effluent of IPC-treated hearts and demonstrated their cardioprotective potential. We here u...
Article
Full-text available
Purpose: High cardiorespiratory capacity is a key determinant of human performance and life expectancy; however, the underlying mechanisms are not fully understood. The objective of this pilot study was to investigate biochemical signatures of endurance-performance athletes using high-resolution nontargeted metabolomics. Methods: Elite long-dist...
Article
Full-text available
Patient-specific cardiomyocytes obtained from induced pluripotent stem cells (CM-iPSC) offer unprecedented mechanistic insights in the study of inherited cardiac diseases. The objective of this work was to study a type 2 long QT syndrome (LQTS2)-associated mutation (c.1600C > T in KCNH2, p.R534C in hERG) in CM-iPSC. Peripheral blood mononuclear cel...
Article
Full-text available
Purpose:: Soccer is one of the most popular sport worldwide, a physical activity of great physiological demand and complexity. Currently, numerous trials involving physiological responses such as hypertrophy, energy expenditure, vasodilation, cardiac output, VO2max, and recovery supported the possibility of genomic predictors affecting performance...
Article
Full-text available
Omics approaches have significantly impacted knowledge about molecular signaling pathways driving cell function. Induced pluripotent stem cells (iPSC) have revolutionized the field of biological sciences and proteomics and, in particular, has been instrumental in identifying key elements operating during the maintenance of the pluripotent state and...
Article
Full-text available
Introduction Hutchinson-Gilford Progeria Syndrome (HGPS) is an extremely rare genetic disorder. HGPS children present a high incidence of cardiovascular complications along with altered metabolic processes and an accelerated aging process. No metabolic biomarker is known and the mechanisms underlying premature aging are not fully understood. Objec...
Article
Aging is followed by numerous physiological limitations that reduce health span, particularly cardiovascular and metabolic disorders. Testosterone supplementation therapy (TST)has been widely used in the treatment of aging dysfunctions in either adult or aged patients, although recent evidence have suggested that the incidence of myocardial infarct...
Preprint
Full-text available
Hutchinson-Gilford Progeria Syndrome (HGPS) is an extremely rare genetic disorder. HGPS children present a high incidence of cardiovascular complications along with altered metabolic processes and accelerated aging process. No metabolic biomarker is known and the mechanisms underlying premature aging are not fully understood. The present study anal...
Article
Full-text available
Metabolomics is an emerging technology that is increasing both in basic science and in human applications, providing a physiological snapshot. It has been highlighted as one of the most wide ranging and reliable tools for the investigation of physiological status, the discovery of new biomarkers and the analysis of metabolic pathways. Metabolomics...
Article
Metabolomics is an emerging technology that is increasing both in basic science and in human applications, providing a physiological snapshot. It has been highlighted as one of the most wide ranging and reliable tools for the investigation of physiological status, the discovery of new biomarkers and the analysis of metabolic pathways. Metabolomics...
Conference Paper
Background: Hutchinson-Gilford Progeria Syndrome (HGPS) is an extremely rare genetic disorder characterized by accelerated aging and high incidence of cardiovascular complications and metabolic alterations. However, the underlying mechanisms of this syndrome are not fully understood. Methods and Results: This study modelled HGPS using cardiomyocyt...
Article
Full-text available
Aging is a complex process that increases the risk of chronic disease development. Hormonal and metabolic alterations occur with aging, such as androgen activity decrease. Studies aim to understand the role of testosterone replacement therapy (TRT) in males, however biomarkers and the metabolic responses to TRT are not well characterized. Therefore...
Article
Cell therapy with mesenchymal stem cells (MSC) has been proposed for the treatment of diabetes mellitus (DM). It is known that the prevalence of thyroid disease is higher among diabetic patients than in general population. Therefore, our aim was to investigate the effect of the treatment with MSC on thyroid function and ROS generation in an experim...
Article
Full-text available
Background Doxorubicin (Dox) is a chemotherapy drug with limited application due to cardiotoxicity that may progress to heart failure. This study aims to evaluate the role of cardiomyocytes derived from mouse embryonic stem cells (CM-mESCs) in the treatment of Dox-induced cardiomyopathy (DIC) in mice. Methods The mouse embryonic stem cell (mESC) li...
Article
Full-text available
Background: Diabetes mellitus is a severe chronic disease leading to systemic complications, including cardiovascular dysfunction. Previous cell therapy studies have obtained promising results with the use bone marrow mesenchymal stromal cells derived from healthy animals (MSCc) in diabetes animal models. However, the ability of MSC derived from d...
Article
Background: Cardiac arrhythmias are one of the main causes of death in ChCP and other dilated cardiomyopathies. Previous studies demonstrated that ventricular arrhythmias are associated with the presence of autoantibodies with beta-adrenergic activity, Ab-β. Objectives: The aim of this study was to investigate whether Ab-β, present in chronic ch...
Article
Diabetes mellitus (DM) encompasses a multitude of secondary disorders, including heart disease. One of the most frequent and potentially life threatening disorders of DM-induced heart disease is ventricular tachycardia (VT). Here we show that toll-like receptor 2 (TLR2) and NLRP3 inflammasome activation in cardiac macrophages mediate the production...
Data
Supplementary Figures 1-11 and Supplementary Tables 1-3.
Article
Full-text available
Rationale: Central questions such as cardiomyocyte subtype emergence during cardiogenesis or the availability of cardiomyocyte subtypes for cell replacement therapy require selective identification and purification of atrial and ventricular cardiomyocytes. However, current methodologies do not allow for a transgene-free selective isolation of atri...
Data
Intracellular and surface marker expression of manually separated and dissociated atria and ventricles. (A) Density plots show representative flow analysis of the cardiomyocyte content in both fractions as indicated by α-actinin staining. (B) Identity of atrial and ventricular cells was analyzed by qRT-PCR with regard to transcript levels of the ve...
Data
Differential expression of ITGA6 in atria and ventricles at various developmental and postnatal stages. (A) Exemplary analysis. Determination of doublets by displaying FSC-A (X-axis) versus FSC-H (Y-axis); all events that are not on the diagonal are considered doublets and excluded from the analysis (NOT-gate, red dashed line). Definition of the ce...
Data
Correlation matrix showing the relationship of gene expression profiles. In four independent experiments embryonic and neonatal mouse heart cells were flow sorted and collected for gene expression analysis. The matrix was generated by unsupervised hierarchical clustering of pair-wise correlation coefficients (Pearson). Correlation coefficients are...
Data
List of tested antibodies, staining conditions and frequencies of the Antibody-based surface marker screening. (XLS)
Data
Temporal expression pattern of ALCAM, VCAM-1 and ERBB-2 in the developing mouse heart. Representative flow analysis of mouse hearts of different developmental stages (E11.5 –P2). Density plots, whole-heart cell suspensions were co-labeled with antibodies against α-actinin (X-axis) and the surface markers ALCAM, VCAM-1 or ERBB-2 (Y-axis). Gates were...
Data
Supplemental methods and materials. (DOC)
Data
List of differentially expressed genes with a higher abundance in embryonic and neonatal ITGA6high sorted cells (alphabetical order). Positive fold change values indicate a higher abundance in ITGA6high as compared to ITGA6low-sorted cells; differential gene expression was defined as a fold change value ≥ 3.0. (XLSX)
Data
List of differentially expressed genes with a higher abundance in embryonic and neonatal ITGA6low sorted cells (alphabetical order). Negative fold change values indicate a higher abundance in ITGA6low as compared to ITGA6high-sorted cells; differential gene expression was defined as a fold change value ≤ -3.0. (XLSX)
Data
List of transcription factor binding sites in the Itga6 core promoter. (XLSX)
Data
Contracting flow sorted ERBB-2+/ITGA6low cells. E15.5 mouse hearts were flow sorted into ERBB-2+/ITGA6low cells (EL) and plated on fibronectin-coated dishes. 10 s videos were recorded from spontaneously beating cells 24 h after plating. (AVI)
Data
Contracting flow sorted ERBB-2+/ITGA6high cells. E15.5 mouse hearts were flow sorted into ERBB-2+/ITGA6high cells (EH) and plated on fibronectin-coated dishes. 10 s videos were recorded from spontaneously beating cells 24 h after plating. (AVI)
Article
Full-text available
We investigated whether the pathways linked to Toll-like receptors 2 and 4 (TLRs) are involved in renal ischemia-reperfusion (I/R)-induced cardiac hypertrophy. Wild type (WT) C57BL/6J, TLR2-/-and TLR4-/-mice were subjected to left kidney ischemia for 60 min followed by reperfusion for 5, 8, 12 and 15 days. Proton density magnetic resonance showed a...
Article
Full-text available
In contrast to mature cardiomyocytes which have limited regenerative capacity, pluripotent stem cells represent a promising source for the generation of new cardiomyocytes. The tendency of pluripotent stem cells to form teratomas and the heterogeneity from various differentiation stages and cardiomyocyte cell sub-types, however, are major obstacles...
Article
Cardiac arrhythmias are one of the main causes of death worldwide. Several studies have shown that inflammation plays a key role in different cardiac diseases and Toll-like receptors (TLRs) seem to be involved in cardiac complications. In the present study, we investigated whether the activation of TLR4 induces cardiac electrical remodeling and arr...
Article
Cardiac arrhythmias are one of the main causes of death worldwide. Several studies have shown that inflammation plays a key role in different cardiac diseases and Toll like receptors (TLR’s) play an important role in cardiac complications. In the present study, we investigated whether the activation of TLR4 induces cardiac electrical remodeling and...
Article
Full-text available
Background Several studies have correlated protein restriction associated with other nutritional deficiencies with the development of cardiovascular and renal diseases. The driving hypothesis for this study was that Ang II signaling pathways in the heart and kidney are affected by chronic protein, mineral and vitamin restriction. Methodology/Princ...
Article
Diabetes mellitus is a serious health problem that can lead to several pathologic complications in numerous organs and tissues. The most important and most prevalent organs affected by this disease are the heart and the kidneys and these complications are the major causes of death in patients with diabetes. MicroRNAs (miRNAs), short non-coding RNAs...
Article
Aims Diabetic patients present a high level of cardiac arrhythmias and risk of cardiac sudden death. The renin–angiotensin system (RAS) plays a key role in diabetes and cardiac diseases. The present study aimed to evaluate whether an angiotensin-converting enzyme 2 (ACE2) activator, diminazene aceturate (DIZE), could improve the streptozotocin (STZ...
Article
Cardiac complications are one of the main causes of death in diabetes. Several studies have shown the anti-diabetic effects of bone marrow mesenchymal stromal cells (MSC). In the present study, we investigated whether MSC-transplantation can revert cardiac dysfunction in streptozotocin-induced diabetic rats and the immunoregulatory effects of MSC w...
Article
Full-text available
Síndrome metabólica é a designação atribuída a um conjunto de alterações fisiológicas no metabolismo humano que aumenta drasticamente o risco de desenvolver doenças cardiovasculares e diabetes, patologias que são as principais causas de morbidez emortalidade atualmente. Um dos grupos frequentemente atingidos é o de crianças e jovensem idade escolar...
Article
Diabetes Mellitus is a metabolic disease characterized by high level of serum glucose. It is a serious health problem that can lead the patient to several physiological complications. According to The American Heart Association, in 2006 Diabetes mellitus killed 72,449 people in the United States along with 17,200,000 U.S. adults have physician-di...

Questions

Questions (4)
Question
Any tips or methods for virus analysis for diagnostics by mass spectrometry?
best sample: Saliva, blood?
best ionization strategy?
Best wishes
Gustavo Monnerat

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Projects (4)
Project
Competition analysis
Project
Mass spectrometry based metabolomics applied for physiology and genetics