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Gustavo Giusiano

Gustavo Giusiano
National University of the Northeast (UNNE)

Doctor

About

116
Publications
39,333
Reads
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1,434
Citations
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April 1993 - present
National University of the Northeast
Position
  • Head of Mycology Department

Publications

Publications (116)
Article
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Purpose of Review SARS-CoV-2 generates an atypical pneumonia with high morbidity and mortality. In many cases, the torpid evolution was related to bacterial or fungal co-infections. Non-specific manifestations of COVID-19 infection can make the differential diagnosis with other systemic diseases even more difficult. A review of systemic endemic myc...
Article
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The Americas are home to biologically and clinically diverse endemic fungi, including Blastomyces, Coccidioides, Emergomyces, Histoplasma, Paracoccidioides, and Sporothrix. In endemic areas with high risk of infection, these fungal pathogens represent an important public health problem. This report aims to summarize the main findings of the regiona...
Article
Malassezia are common yeasts in human skin microbiome. Under certain conditions these yeasts may cause disease from skin disorders to systemic infections. In the absence of clinical breakpoints, epidemiological cutoff values (ECVs) are useful to differentiate isolates with acquired or mutational resistance. The aim of this work was to propose tenta...
Article
COVID-19 associated pulmonary aspergillosis (CAPA) incidence varies depending on the country. Serum galactomannan quantification is a promising diagnostic tool since samples are easy to obtain with low biosafety issues. A multicenter prospective study was performed to evaluate the CAPA incidence in Argentina and to assess the performance of the lat...
Article
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The genus Lichtheimia is the third-most frequent genus isolated in mucormycosis. We report a cutaneous case caused by Lichtheimia ramosa, localized on the face of a pediatric patient, in the context of acute liver failure and caloric malnutrition. Several surgeries and treatment with liposomal amphotericin B enabled the patient's favorable evolutio...
Article
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Combining traditional medicine with nanotechnology therefore opens the door to innovative strategies for treating skin and soft tissue infections (SSTIs) and also contributes to the fight against the rise of antimicrobial resistance. Acanthospermum australe (Loefl.) Kuntze is a medicinal plant used by indigenous peoples in northeastern Argentina to...
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Resumen Evaluamos las concordancias interlaboratorio, esencial y categórica entre el panel Sensititre YeastOneTM y los métodos de referencia correspondientes al M27 4.ª ed. (Clinical and Laboratory Standards Institute [CLSI]) y al EDef 7.3.1 (European Committee on Antifungal Susceptibility Testing [EUCAST]). Estudiamos 67 cepas de Candida de distin...
Article
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Paracoccidioidomycosis (PCM) is the most relevant systemic endemic mycosis limited to Latin American countries. The etiological agents are thermally dimorphic species of the genus Paracoccidioides . Infection occurs via respiratory tract by inhalation of propagules from the environmental (saprophytic) phase. In the lung alveoli the fungus converts...
Chapter
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Ethno-phytopharmacology studies the traditional use of plants for the prevention and cure of several diseases. It provides multidisciplinary research on components of medicinal plants, their identification and description, properties, modes of action and interactions with the human organism. Search for new bioactive drugs is another aim of these ex...
Article
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The genus Malassezia comprises a heterogeneous group of species that cause similar pathologies. Malassezia yeasts were considered as the most abundant skin eukaryotes of the total skin mycobiome. The ability of this fungus to colonize or infect is determined by complex interactions between the fungal cell and its virulence factors. This study aims...
Article
Paracoccidioidomycosis (PCM) is a life-threatening systemic mycosis widely reported in the Gran Chaco ecosystem. The disease is caused by different species from the genus Paracoccidioides, which are all endemic to South and Central America. Here, we sequenced and analyzed 31 isolates of Paracoccidioides across South America, with particular focus o...
Article
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Background: Candida auris is an emerging yeast frequently reported as resistant to multiple antifungal drugs commonly used to treat Candida infections. This specie can colonize the patient's skin and has great ability for producing outbreaks in hospitals. C. auris is phylogenetically related to other Candida species, can be misidentified using con...
Article
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Introducción: La pitiriasis versicolor es una patología frecuente en Paraguay; sin embargo, su epidemiologia es desconocida. Objetivo: Determinar la frecuencia de especies de Malassezia causantes de pitiriasis versicolor y las características epidemiológicas de la población. Materiales y Métodos: Se recolectaron muestras de pacientes con diagnóst...
Article
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Malassezia furfur is lipophilic and lipid-dependent yeast, inhabitant of human skin microbiota associated with several dermal disorders. In recent years, along with the advances in nanotechnology and the incentive to find new antimicrobial drugs, there has been a growing interest in the utilization of nanoparticles for the treatment of skin microbi...
Article
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p class="Default">En la práctica médica tienen cada vez más importancia las enfermedades causadas por hongos oportunistas adquiridas en ambientes hospitalarios. La mayor gravedad se presenta en lugares de alto riesgo, como guarderías, residencias de ancianos, quirófanos y Unidades de Terapia Intensiva (UTI)(1) . En los ambientes internos la prolife...
Article
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Mucorales are resistant to most antifungals. Mucormycosis associated mortality is unacceptable and new treatment approaches are needed. The objectives of this work were (i) to evaluate the nature and intensity of the in vitro effect of three drugs combinations which included voriconazole (plus amphotericin B, posaconazole and caspofungin) against 2...
Article
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Purpose of Review Paracoccidioidomycosis (PCM) is one of the most prevalent systemic endemic mycosis in Latin America caused by species of an environmental fungus of the genus Paracoccidioides. In Argentina, the endemic area with the highest incidence is located in the Northeast. This review presents the current aspects of PCM epidemiology influenc...
Chapter
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For this chapter, a literature search of PubMed/ScienceDirect was performed on January 20th, 2017 using the following search terms: "essential oils AND skin conditions AND treatment". The authors (PM-AOM) screened titles and abstracts for relevance. Articles that did not have an abstract or full text available in English or Spanish were excluded. T...
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Hereby we communicate the first autochthon isolation of Cryptococcus gattii VGIII in Chile, which was obtained from a nasal injury in a domestic cat in the Valparaíso region in Chile. The strain was identified using morphophysiological tests, MALDI-TOF, and URA5 gene PCR-RFLP analysis.
Article
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The composition of the essential oils (EOs) of O. vulgare L. EO and T. vulgaris EO, were analyzed by GC and GC–MS. Antifungal activities of the EOs and its main component, carvacrol, were evaluated against 27 clinical isolates of Malassezia furfur. Minimum inhibitory concentrations (MICs) were measured according to the broth microdilution protocols...
Article
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Argentina has two endemic areas of paracoccidioidomycosis (PCM). Bordering Paraguay and Brazil, Northeast Argentina (NEA) comprises the area with the highest incidence where the chronic adult clinical form has historically been reported. Juvenile form in children and adolescents is rare in this area since only one case was reported in the last 10 y...
Article
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A case of cavitary pulmonary sporotrichosis without mucocutaneous involvement caused by Sporothrix schenckii is reported in a sexagenarian woman with a long smoking history. The patient was hospitalized for septic shock with multiorgan failure from a respiratory focus. The diagnosis was delayed due to the fungal etiological agent was not initially...
Article
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The degree of antagonism exercised by fungi on geohelminth development varies according to the morphological alterations caused by different fungal species. Saprophytic fungi may exert ovicidal or ovistatic effects. The aim of this study was to apply scanning electron microscopy (SEM) to observe the action of two soil saprophytic species of Chrysos...
Article
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Introduction: Conyza bonariensis (L.) Cronquist is a herbaceous plant of the underbrush group. It is distributed worldwide and is used to treat a variety of skin conditions. Objective: Evaluate the in vitro inhibitory activity of the tincture from C. bonariensis leaves against fungi and bacteria causing superficial infections. Methods: The tincture...
Article
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A total of 59 C. parapsilosis sensu stricto and 1 C. orthopsilosis recovered from catheters and blood cultures of pediatric patients of the Northeastern region of Argentina were studied. Susceptibility to azoles, amphotericin B, and echinocandins were tested by broth microdilution method. According to CLSI clinical breakpoints, more than 91% of the...
Article
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The distribution of Aspergillus species in soil has been widely studied all over the world. The aim of this study was the phenotypic and genotypic characterization of species Aspergillus belonging to section Fumigati present in soils from two Argentinian semi-desert areas having different geological conditions. Altogether, 23 isolates belonging to...
Article
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Human protothecosis is a rare infection caused by algae of the genus Prototheca. Prototheca wickerhamii has been recognized as the main species that causes infection in immunocompromised hosts with deficits in innate or cellular immunity. We report a case of persisting subcutaneous protothecosis in a patient with T-cell large granular lymphocyte le...
Article
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Malassezia spp. are lipophilic yeasts not only present in the normal skin microflora, but also responsible of skin-related diseases (pityriasis versicolor, seborrheic/atopic dermatitis and dandruff) as well as systemic fungal infections in humans and animals. Their treatment and eradication are mainly based on old azole drugs, which are characteriz...
Article
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Paracoccidioidomycosis is an endemic mycosis caused by Paracoccidioides species limited to Latin America arising with the chronic form in 90% of cases. The capacity of microorganisms to form biofilms is considered of great importance medical since can contribute to the persistence and to the chronic state of the diseases. The ability of Paracoccidi...
Article
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Mediante la técnica del anzuelo queratínico (crin de caballo y pelos de niños), se aislaron 27 muestras de suelo de áreas recreacionales y areneros de jardines de infantes de la ciudad de Resistencia (Argentina). El 100% de las muestras fueron positivas a hongos queratinofílicos, aislandose 15 géneros y 27 especies. Los géneros de mayor prevalencia...
Article
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Malassezia species are natural inhabitants of the healthy skin. However, under certain conditions, they may cause or exacerbate several skin diseases. The ability of this fungus to colonize or infect is determined by complex interactions between the fungal cell and its virulence factors. This study aims to evaluate “in vitro” the hydrophobicity lev...
Article
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Se describen, comentan e ilustran 6 especies de hongos mitospóricos hialinos y dematiáceos desde muestras de suelos y substratos leñosos. Una de ellas es una cepa considerada una variante de mayores dimensiones conidiales de Bipolaris neergardii, obtenida de la zona del desierto de Atacama (Norte de Chile), las restantes: Sesquicillium candelabrum,...
Article
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All Malassezia species are lipophilic; thus, modifications are required in susceptibility testing methods to ensure their growth. Antifungal susceptibility of Malassezia species using agar and broth dilution methods has been studied. Currently, few tests using disc diffusion methods are being performed. The aim was to evaluate the in vitro suscepti...
Article
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Durante el año 2003 en diversas investigaciones en curso relacionadas con la la micota epífita de Pinus canariensis de la zona de Misiones (Argentina), la queratinofílica y la endófita de la zona central de Chile,han permitido reunir un grupo interesante de hongos integrantes de 5 géneros, que merecen ciertos comentarios ya sea por ser poco comunes...
Article
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Con el objetivo de estudiar los microhongos anemófilosde las ciudades de Resistencia y Corrientes, ubicadas en el nordeste argentino, se muestrearon por la técnica de depositación gravitacional, dos zonas en cada ciudad. El muestreo se realizó en primavera y otoño, en dos horarios diferentes. Existen diferencias entre los aislamientos de ambas ciud...
Article
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This paper can be considered as a little constribution to knowledge of fungal biodiversity in Namibia. Soils, seed dung and some vegetal substrata such as herb, leaves and bark were collected at random in order to investigate the presence of tropical microfungi and to determine the significance of some fungal taxa in the Namibian ecosystem.A total...
Article
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Se describen los aspectos morfológicos de Thozetella tocklaiensis, un raro hongo anamórfico aislado en la litera vegetal senesente de Pinus elliottii en la provincia de Misiones (Argentina), mediante el empleo de diferentes medios de cvultivo (PCA, PDA, CMA y OA), con o sin adición de substratos vegetales tales como trozos estériles de tallos de Lo...
Article
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Palabras clave:Microhongos oportunistas, litera de coníferas, salud pública. RESUMEN Se analizó la colonización in vitro de microhongos filamentosos de rápido crecimiento en la litera de conífe-ras (Pinus elliottii) en el N.E argentino, mediante mues-tras estacionales de acículas senescentes de 4 localida-des con semejanzas climáticas, edáficas y v...
Article
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Current therapy for mycoses is limited to the use of a relative reduced number of antifungal drugs. Although amphotericin B still remains considered as the "gold standard" for treatment, acute and chronic toxicity, such as impairment of renal function, limits its use and enhances the investigation and clinical use other chemical families of antifun...
Article
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We studied the in vitro antifungal activity profile of amorolfine (AMR), bifonazole (BFZ), clotrimazole (CLZ), econazole (ECZ), fluconazole (FNZ), itraconazole (ITZ), ketoconazole (KTZ), miconazole (MNZ), oxiconazole (OXZ), tioconazole (TCZ) and terbinafine (TRB) against 26 clinical isolates of Scopulariopsis brevicaulis from patients with onychomy...
Article
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Background: The soil is a natural reservoir of keratinophilic fungi, which are a small but important group of filamentous fungi, some of which typically develop on keratinized tissues of living animals. There are numerous species of saprophytic fungi with recognized keratinophilic abilities, and several studies have been undertaken in order to lin...
Article
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According with CLSI document M38-A2 the antifungal susceptibility of 40 clinical and environmental isolates of A. terreus sensu stricto against amphotericin B, terbinafine, itraconazole and voriconazole was performed. For itraconazole and voriconazole all isolates showed lower MICs than epidemiologic cutoff values and the 5% of the isolates present...
Article
Background: The use of different isolation techniques allows the recovery of fungi based on their ability to use selective substrates. The sprinkle method is a technique for the recovery of nematophagous fungi in the soil. These fungi are natural predators of nematodes and are widely distributed in nature. Aims: To detect possible fungi with nem...
Article
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We report a case of mycotic keratitis caused by Lasiodiplodia theobromae in a 60-year-old man with a history of ocular trauma with vegetable matter. Ophthalmological assessment with slit-lamp and microbiological evaluation of the corneal ulcer by conventional microbiological techniques were performed. Mycology study of the corneal scraping showed t...
Article
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We report a case of mycotic keratitis caused by Lasiodiplodia theobromae in a 60-year-old man with a history of ocular trauma with vegetable matter. Ophthalmological assessment with slit-lamp and microbiological evaluation of the corneal ulcer by conventional microbiological techniques were performed. Mycology study of the corneal scraping showed t...
Article
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We studied the in vitro activity of fluconazole (FCZ), ketoconazole (KTZ), miconazole (MCZ), voriconazole (VCZ), itraconazole (ITZ) and amphotericin B (AMB) against the three major pathogenic Malassezia species, M. globosa, M. sympodialis, and M. furfur. Antifungal susceptibilities were determined using the broth microdilution method in accordance...
Article
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A case of fungemia with interstitial lung compromise caused by Malassezia sympodialis is reported in an obese pediatric patient on long-term treatment with inhaled corticosteroids for asthma. The patient was hospitalized due to a post-surgical complication of appendicitis. The patient was treated with amphotericin B for 3 weeks, with good clinical...
Article
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Aspergillus is a group of opportunistic fungi that cause infections, with high morbidity and mortality in immunosuppressed patients. Aspergillus fumigatus is the most frequent species in these infections, although the incidence of other species has increased in the last few years. To evaluate the air fungal load and the diversity of Aspergillus spe...
Article
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Background Seborrheic dermatitis (SD) is considered the second most frequently dermatosis associated with the genus Malassezia but little is the knowledge about the epidemiology of this association. Aims To determinate the prevalence of Malassezia species associated with SD and to analyse their distribution according to the location of the lesion...
Article
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Cryptococcosis is a fungal infection caused by yeast species of Cryptococcus genus, particularly Cryptococcus neoformans/Cryptococcus gattii species complex. The knowledge of the cryptococcosis casuistic in northeastern Argentina is scarce and there is no information about the molecular types circulating in this area. The aim of this study was to g...
Article
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In Argentina, information about epidemiology and environmental distribution of Cryptococcus is scarce. The city of Resistencia borders with Brazil and Paraguay where this fungus is endemic. All this supported the need to investigate the ecology of the genus and the epidemiology of cryptococcosis in this area. The aim was to investigate the presence...
Article
Full-text available
Cryptococcosis is a fungal infection caused by yeast species of Cryptococcus genus, particularly Cryptococcus neoformans/Cryptococcus gattii species complex. The knowledge of the cryptococcosis casuistic in northeastern Argentina is scarce and there is no information about the molecular types circulating in this area. The aim of this study was to g...
Article
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Sertaconazole is a useful antifungal agent against mycoses of the skin and mucosa, such as cutaneous, genital and oral candidiasis and tinea pedis. Its antifungal activity is due to inhibition of the ergosterol biosynthesis and disruption of the cell wall. At higher concentrations, sertaconazole is able to bind to nonsterol lipids of the fungal cel...
Article
Background Dermatophytes can be divided into geophilic (soil), zoophilic (animals) and anthropophilic (humans) strains, depending on the source of the keratin that they use for nutritional purposes.AimsThe in vitro susceptibility of clinical isolates of dermatophyte fungi has been studied in the 3 ecological groups with several antifungal agents fo...
Article
The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of Malassezia species against...
Article
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The incidence of onychomycosis due to non-dermatophyte moulds (NDM) is increasing. Aspergillus terreus is relatively undocumented as an agent of this fungal infection. The aim of this work is to show the prevalence of onychomycosis caused by A. terreus and to describe its clinical features. Nail samples were collected for microscopic examination an...
Article
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Background Aspergillus is a group of opportunistic fungi that cause infections, with high morbimortality in immunosuppressed patients. Aspergillus fumigatus is the most frequent species in these infections, although the incidence of other species has increased in the last few years. Aims To evaluate the air fungal load and the diversity of Aspergi...
Article
Full-text available
Antifungal activity and in vitro inhibition time for sertaconazole (STZ) and 9 other topical drugs, namely amorolfine, bifonazole, clotrimazole, econazole, ketoconazole, miconazole, oxiconazole, terbinafine, and tioconazole were determined against 124 clinical isolates of dermatophyte (12 species) fungi by the microdilution method in a liquid mediu...
Article
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Background Onychomycosis accounts for up to 50% of all nail disorders. They can be caused by: yeasts, dermatophytes and non-dermatophyte moulds.Objectives and methodsA multicentre study designed to determine the prevalence, mycological test results, aetiological agents, and clinical presentation of onychomycosis was carried out. All fingernail and...
Article
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Onychomycosis accounts for up to 50% of all nail disorders. They can be caused by: yeasts, dermatophytes and non-dermatophyte moulds. A multicentre study designed to determine the prevalence, mycological test results, aetiological agents, and clinical presentation of onychomycosis was carried out. All fingernail and toenail samples taken during a o...
Article
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The fungistatic and fungicidal activities of sertaconazole against dermatophytes were evaluated by testing 150 clinical isolates of causative agents of tinea pedis, Trichophyton rubrum, Trichophyton mentagrophytes, and Epidermophyton floccosum. The overall geometric means for fungistatic and fungicidal activities of sertaconazole against these isol...