Gulmira Ozek

Anadolu University · Department of Pharmacognosy

The family Asteraceae comprises many species that have medicinal importance in terms of their chemical components. Some species of the genus Lactuca have been used in folk medicine for a long time ago. One of them is L. serriola L., a wild plant that is a weed in agriculture. To date, few studies have been published on its chemical profile. In this...

Propolis is a resinous mixture of substances collected and processed from various botanical sources by honeybees. Black poplar (Populus balsamifera L.) buds are one of the primary sources of propolis. Despite their reported therapeutic properties, little is known about the innate immunomodulatory activity of essential oils from P. balsamifera and p...

Grindelia squarrosa (Pursh) Dunal is used in traditional medicine for treating various diseases; however, little is known about the immunomodulatory activity of essential oils from this plant. Thus, we isolated essential oils from the flowers (GEOFl) and leaves (GEOLv) of G. squarrosa and evaluated the chemical composition and innate immunomodulato...

Comparative study GC-FID/MS of essential oils of fruits, leaves and roots of the endemic plant Angelica panciciiVandas ex Velen. revealed a significant difference in their chemical composition. The enantiomeric purity of the main component in the fruit oil (+)-β-phellandrene was also confirmed. In addition, imperatorin, isoimperatorin, oxypeucedani...

Despite their reported therapeutic properties, not much is known about the immunomodulatory activity of essential oils present in Artemisia species. We isolated essential oils from the flowers and leaves of five Artemisia species: A. tridentata, A. ludoviciana, A. dracunculus, A. frigida, and A. cana. The chemical composition of the Artemisia essen...

Black cumin,Nigella sativaL. belonging to Ranunculaceae, is a valuable medicinal plant because of the curative effects of seed and oil. Despite studieson black cumin, there are few studies on the effects of nitrogen on fatty acid composition, no records on thymoquinone yield, and the thymoquinone content of the seed oil. In this study, the effects...

Little is known about the immunomodulatory activity of essential oils isolated from Juniperus species. Thus, we isolated essential oils from the cones and leaves of eight juniper species found in Montana and in Kazakhstan, including J. horizontalis, J. scopolorum, J. communis, J. seravschanica, J. sabina, J. pseudosabina, J. pseudosabina subsp. tur...

The genus Berberis (Berberidaceae) is present in Kazakhstan Flora by eight species, two of which Berberis karkaralensis Kornil. & Potapov and B. Iliensis are listed in the Red Book of Kazakhstan. But, the phytochemical studies of Kazakhstan barberry species conducted is insufficient until today. This paper presents the phytochemical analysis and an...

Rhododendron (Ericaceae) extracts contain flavonoids, chromones, terpenoids, steroids, and essential oils and are used in traditional ethnobotanical medicine. However, little is known about the immunomodulatory activity of essential oils isolated from these plants. Thus, we isolated essential oils from the flowers and leaves of R. albiflorum (casca...

The effects of two Solanum vegetables, S. macrocarpon L. (African eggplant), and S. nigrum L. (black nightshade) on aluminum model of neurodegeneration in Drosophila melanogaster was investigated. Flies were treated with AlCl3 alone or in combination with the leaves from both samples in their diets for 7 days. Thereafter, locomotor performance and...

In this study two different extraction techniques namely, conventional hydrodistillation (HD) and micro-steam distillation-solid-phase microextraction (MSD-SPME), were used to analyze the volatile constituents from the leaves and the galls of Salvia fruticosa Mill. by gas chromatography (GC-FID) and gas chromatography coupled to mass spectrometry (...

Hypericum L. (Hypericaceae) extracts have been used for their therapeutic effects; however, not much is known about the immunomodulatory activity of essential oils extracted from this plant. We isolated essential oils from the flowers and leaves of H. perforatum and analyzed their chemical composition and innate immunomodulatory activity. Analysis...

In this study the volatile components of four Inula species (Inula germanica L., I. bifrons (L.) L., I. ensifolia L., and I. salicina L.) were analysed by GC-FID/MS. A total of 141 chemical compounds were identified. A distinct volatile chemical profiles with high variation in the type of compounds was observed. Inula germanica was rich in oxygenat...

In the present study, the leaf anatomical structure and essential oil chemical composition of Cordia myxa (Boraginaceae) growing in Turkey were investigated. In the anatomical study of C. myxa conducted by light microscopy, anomocytic stomata, cystoliths, cystolithic trichomes, glandular trichomes, and calcium oxalate crystals were observed in the...

In the current work the flowers of green and dark purple Ocimum basilicum L. varieties cultivated in Uzbekistan were comparatively studied in terms of chemical composition of the lipids, essential oils and fatty acids by using TLC, GC-FID and GC-MS techniques. The lipid composition of flowers both basil varieties comprised biologically active subst...

The methanol extract of the Balkan endemic species Jurinea tzar-ferdinandii Davidov demonstrated weak antioxidant activity against DPPH• and ABTS+• and low inhibitory potential against acetylcholinesterase (8.3% Inh.) and tyrosinase (IC50 = 208 ± 8 µg/mL) enzymes. Phytochemical investigation of the extract led to isolation and identification of api...

Chlorogenic (5‐CQA), 1,5‐, 3,5‐, 4,5‐ and 3,4‐dicaffeoylquinic (DCQA) acids were identified and quantified in the methanol extracts of Inula oculus‐christi L., I. bifrons L., I. aschersoniana Janka var. aschersoniana, I. ensifolia L., I. conyza (Griess.) DC. and I. germanica L. by HPLC analysis. The amount of 5‐CQA varied from 5.48 to 28.44 mg/g DE...

In the present work, chemical compositions of essential oil and methanol extract of endemic Cota fulvida (Grierson) Holub were investigated as well as their antioxidant, antidiabetic, antiinflammatory and antimelanogenesis potent. The phytochemical analyses have been performed with GC-MS/FID and LC-MS/MS techniques. The essential oil was characteri...

In the present study, the extract of an endemic plant ourkey Marrubium sivasense Aytaç, Akgül & Ekici was investigated for phytochemical profile, antioxidant properties and inhibition of several enzymes. The chemical composition of the extract was investigated with LC-MS/MS technique. Forsythoside B, verbascoside and leucoseptoside were identified...

Hexane extracts of Heracleum verticillatum, H. sibiricum, H. angustisectum and H. ternatum were studied for their furanocoumarin content antioxidant potential and acetylcholinesterase and α‐amylase inhibitory activities. Quantification of the furanocoumarins was performed by 1H NMR. Pimpinellin was found to be the main component in the roots of all...

In the present work, lipids and essential oil constituents of endemic Cota hamzaoglui Özbek & Vural were investigated with GC-FID/MS techniques. The fatty acids fraction was isolated with liquid-liquid extraction from the herb with Folch method and then methylated with BF3 reagent. Linolenic, linoleic, oleic and hexadecanoic acids were found to be...

Currant and gooseberry, the genus Ribes, is represented by eight species in the Flora of Turkey. We examined structures of glandular trichomes and composition of the essential oils from leaves of five Ribes species. The glandular trichomes were investigated by light and scanning electron microscopy. The glandular hairs with multicellular stalk and...

In this study, essential oil properties of an endemic plant of Turkey, Coucinia sivasica Hub.-Mor. were studied for the first time. Essential oil of C. sivasica was obtained by using Clevenger apparatus with hydrodistillation technique. The essential oil was analyzed by GC-FID and GC/MS techniques on a polar column and total 44 compounds were ident...

Essential oils (EOs) were obtained by hydrodistillation of various parts of Ferula ovina (Boiss.) Boiss., Ferula iliensis Krasn. ex. Korovin, and Ferula akitschkensis B. Fedtsch. ex Koso-Pol., collected in the flowering/budding and fruiting stages. Eight samples of EOs isolated from F. ovina and four samples from F. akitsckensis were analyzed by ga...

Pentzia incana is one of the most important of all natural pasture plants in the dry interior (Karoo) region of South Africa. This highly aromatic shrub is thought to be responsible for the distinctive flavour of Karoo lamb (a registered geographical indication), yet the essential oil is here characterised for the first time. Leafy twigs are tradit...

In the present work, an endemic species Prangos heyniae collected in four locations from Turkey was subjected to hydrodistillation in Clevenger type apparatus to obtain the essential oils (EO1-4). The gas-chromatography/mass spectrometry (GC/MS) and gas-chromatography-flame ionization detector (GC/FID) analyses showed that the EOs were rich in sesq...

Two samples of Tanacetum praeteritum (Horwood) Heywood subsp. praeteritum (Horwood) were collected in flowering period and subjected separately to hydrodistillation to yield the essential oils (A and B). The oils were investigated for chemical composition with GC-FID and GC/MS techniques and evaluated against acetylcholinesterase and α-amylase enzy...

In the present work, the efficiency of different nitrogen doses (0, 50, 100, 150, and 200 kg ha⁻¹) on growth, yield, and quality of stevia (Stevia rebaudiana Bert.) was investigated in 2011–2013. The study was conducted in Antalya located in the Mediterranean Region of Turkey. Terra rossa type soil (LVx, FAO) characteristics of the experimental fie...

In the present work, fruits of Prangos platychlaena Boiss. ex Tchihat. ssp. platychlaena (Apiaceae) collected from eastern (sample A) and central (sample B) parts of Turkey were subjected to hydrodistillation to yield essential oils (EOs). The chemical composition of P. platychlaena ssp. platychlaena fruit oil was analyzed by gas chromatography–fla...

The phytochemical composition of essential oils and extracts of Tanacetum haussknechtii were investigated with GC–FID–MS and LC–MS/MS techniques and evaluated against oxidation, acetylcholinesterase, tyrosinase, and α-amylase enzymes. The major volatile constituents of T. haussknectii were found to be α- and β-pinene, and borneol. Caffeoylquinic ac...

The aim of the research was to investigate the chemical composition of essential oils and volatiles from two species of Artemisia glabella and Artemisia rupestris growing in Kazakhstan. Two different techniques, the conventional hydrodistillation (HD) and modern fast micro-steam distillation-solid-phase microextraction (MSD-SPME) have been used to...

Two different isolation techniques, conventional hydrodistillation (HD) and micro-steam distillation-solid-phase microextraction (MSD-SPME), have been used to analyze the volatile constituents from the leaves and rhizomes of Acorus calamus L. by gas chromatography (GC-FID) and gas chromatography coupled to mass spectrometry (GC-MS). In all the samp...

The essential oils obtained by hydrodistillation from Pegolettia baccharidifolia Less. and P. retrofracta (Thunb.) Kies were studied for the first time, using GC/MS and GC/FID techniques. A total of 178 components were identified, representing between 85.3% and 100.0% of the total oils. Some differences were observed in the composition of the essen...

The present study is the first report on essential oil composition, phytochemicals and biological potential of A. sivasica tested against free radicals, oxidative damage and tyrosinase enzyme. GC-FID and GC/MS analyses revealed that α-pinene (11.5%, 9.3% and 6.7%), β-pinene (7.0%, 3.0% and 6.9%), 1,8-cineole (18.0%, 22.1% and 6.7%) and camphor (7.6...

Essential oil extracts from Ferula iliensis have been used traditionally in Kazakhstan for treatment of inflammation and other illnesses. Because little is known about the biologic activity of these essential oils that contributes to their therapeutic properties, we analyzed their chemical composition and evaluated their phagocyte immunomodulatory...

In the present work, Microsteam distillation - Solid phase microextraction (MSD-SPME) and hydrodistillation (HD) techniques were applied to obtain volatiles from Achillea sivasica, an endemic species from Turkey. GC-FID and GC/MS analysis revealed that 1,8-cineole (22.1%) and α-pinene (9.3%) were the main constituents of the hydrodistilled flower v...

The aim of this study is to investigate essential oil composition of the flowers of 14 Carthamus tinc-torius L. (Safflower - Asteraceae) lines and varieties developed selection breeding method and their reaction to rainfed and irrigated conditions. Essential oil was extracted from dried flowers by SPME, which were then analyzed by using GC-FID and...

The chemical composition and mosquito larvicidal and adulticidal activity of five essential oils (EOs), Dracocephalum ruyschiana L. (DR), D. foetidum Bunge (DF), D. moldavica L. (DM), D. fruticulosum Steph. ex Willd. (DFr) and D. peregrinum L. (DP) were evaluated. Simultaneous GC-FID and GC-MS analyses revealed in the EOs of DR, DF and DM an uniden...

Leaves of Juglans regia L. collected from two different locations [Adana (A) and Ankara (B)] from Turkey were subjected to hydrodistillation in a Clevenger type apparatus to yield the essential oils (EOs). GC/MS and GC-FID analyses revealed that the A EO was rich in thymol (23.1%), while caryophyllene oxide (33.8%) was found as the main constituent...

Essential oils were obtained by hydrodistillation of the umbels+seeds and stems of Ferula akitschkensis (FAEOu/s and FAEOstm, respectively) and analyzed by gas chromatography and gas chromatography-mass spectrometry. Fifty two compounds were identified in FAEOu/s. The primary components were sabinene, α-pinene, β-pinene, terpinen-4-ol, eremophilene...

A detailed analysis of Prangos pabularia Lindl. (Apiaceae) fruit oil was performed by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). Bicyclogermacrene (21%), (Z)-β-ocimene (19%), α-humulene (8%), α-pinene (8%) and spathulenol (6%) were the main constituents of the oil. One compound with 1.8% at RI 3420 remained uniden...

Air dried aerial parts of Stachys iberica subsp. iberica were subjected to water-distillation using a Clevenger-type system. The resulting essential oil was analysed by GC-FID and GC-MS, simultaneously. Overall, thirty components were characterized. Hexadecanoic acid (41.5%), %), phytol (8.2%) and germacrene D (9.7%) were characterized as major con...

Определена антимикробная активность 15 образцов эфирных масел, выделенных из 9 видов растений Казахстана: Artemisia kotuchovii Kupr., Artemisia scoparia Waldst. et Kit., Artemisia absinthium L., Ferula iliensis Krasn. ex Korovin, Ferula ovina (Boiss.) Boiss., Heracleum dissectum Ldb., Angelica decurrens (Ledeb.) B. Fedtsch., Thymus marschallianus W...

Essential oils were obtained by hydrodistillation of flowers+leaves and stems of A. kotuchovii Kupr. (AKEOf+l and AKEOstm, respectively) and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS). The primary components of the oils were estragole, (E)- and (Z)-β-ocimenes, methyl eugenol, limonene, spathulenol, β-pinene...

The essential oils from the flower, leaf, and stem of Echinophora lamondiana B.Yildiz et Z.Bahcecioglu were analyzed by gas chromatography-flame ionization detection and gas chromatography-mass spectrometry. In total, 41, 37, and 44 compounds were identified, which accounted for 98.0, 99.1, and 97.0% of flower, leaf, and stem essential oils, respec...

Mosquitoes are vectors for many pathogens that cause human diseases including dengue fever, yellow fever and malaria. In an effort to discover new plant based products, essential oils from the flower, leaf and stem of Echinophoralamondiana B.Yildiz et Z.Bahcecioglu were screened for their larvicidal and repellent activity against mosquitoes. Only l...

In the present work three subspecies of Helichrysum plicatum DC. (Helichrysum plicatum DC. subsp. plicatum, Helichrysum plicatum DC. subsp. polyphillum (Ledeb) P.H.Davis & Kupicha and Helichrysum plicatum DC. subsp. isauricum Parolly) were investigated for the essential oil chemical compositions. The volatiles were obtained by conventional hydrodis...

Artemisia argyi, A. feddei, A. gmelinii, A. manshurica, and A. olgensis (Asteraceae) were collected in Far East Russia. Oils were hydrodistilled and simultaneously analyzed by GC-FID and GC/MS. Main constituents were found as follows in Artemisia oils: selin-11-en-4a-ol (18.0%), 1,8-cineole (14.2.0%), artemisia alcohol (12.9%), borneol (9.7%) in A....