Guillermo Pastor-Palacios

• Ph.D.
• Investigador Cátedra CONACYT at Instituto Potosino de Investigación Científica y Tecnológica

[This corrects the article DOI: 10.3389/fcimb.2018.00214.].

Salvia circinnata Cav. (Syn. S. amarissima Ortega), Lamiaceae, is a Mexican medicinal plant used in traditional medicine for the treatment of gastrointestinal illness, cancer, and diabetes. Samples of ten natural populations were analyzed by application of a systematic approach which involved the combination of DNA barcoding, GC–MS, HPLC–DAD, and H...

Pecan (C. illinoinensis) pollen is an important cause of allergic respiratory disease. Pecan is distributed worldwide as shade, ornamental or cultivation tree. To date three well known pecan food allergens have been reported, however, pollen allergens have not been identified. Here, we describe the first identification of IgE recognized pecan polle...

Geminiviruses have genomes composed of single-stranded DNA molecules and encode a rolling-circle replication (RCR) initiation protein (“Rep”), which has multiple functions. Rep binds to specific repeated DNA motifs (“iterons”), which are major determinants of virus-specific replication. The particular amino acid (aa) residues that determine the pre...

Salvia connivens Epling, Lamiaceae, is an annual herbaceous plant used as a traditional medicine in Mexico for the treatment of diarrhea, which out can be caused by bacterial, viral, and protozoal infections, as well as toxins mainly. Mexican medicinal plants with anti-diarrheic activity represent a source of potential antiprotozoal agents. The phy...

Ephedra is one of the largest genera of the Ephedraceae family, which is distributed in arid and semiarid regions of the world. In the traditional medicine from several countries some species from the genus are commonly used to treat asthma, cold, flu, chills, fever, headache, nasal congestion, and cough. The chemical constituents of Ephedra specie...

Both DNA barcoding and phylogenetic data of the studied botanical material suggested the existence a new population of Galphimia glauca. Their leaves afforded three new nor-3,4-seco-friedelanes named galphimines M-O, together with known galphimines D, E, G, and I. Galphimines M and N possess bicyclic orthoacetates which are the first examples of or...

The begomoviruses (BGVs) are plant pathogens that evolved in the Old World during the Cretaceous and arrived to the New World (NW) in the Cenozoic era. A subgroup of NW BGVs, the “Squash leaf curl virus (SLCV) lineage” (S-Lin), includes viruses with unique characteristics. To get clues on the evolutionary origin of this lineage, a search for diverg...

A novel bipartite begomovirus, Blechum interveinal chlorosis virus (BleICV), was characterized at the genome level. Comparative analyses revealed that BleICV coat protein (CP) gene promoter is highly divergent from the equivalent region of other begomoviruses (BGVs), with the single exception of Tomato chino La Paz virus (ToChLPV) with which it sha...

Begomoviruses exhibiting TACE spacer sequences identical or similar to the CLE core. (PDF)

Experimental data of GUS activity presented in Figs 7, 9 and 10, and S3 Fig. (XLSX)

Expression of different CLE-containing CP promoters in presence of EuMV factors. (PDF)

Symptoms caused by BleICV in Blechum pyramidatum plants. (PDF)

Names, acronyms and GenBank accession numbers of geminiviruses compared in Fig 1. (PDF)

TACE and associated putative cis-acting elements in four geminiviruses unassigned to a genus. (PDF)

CP promoter region with the TACE-CLE-TATA box conserved arrangement in members of the SLCV lineage. (PDF)

Oligonucleotides used in this study. (PDF)

CP promoter region containing the inverted CLE-TATA box-TACE arrangement in members of the ToLCNDV lineage. (PDF)

Complete V2/CP promoter of selected Sweepoviruses, a divergent lineage of Old World begomoviruses. (PDF)

Eleven neo-clerodane diterpenoids (1–11) including the new analogues 1, 2, and 10, and 3′,5,6,7-tetrahydroxy-4′-methoxyflavone (12) were isolated from the aerial parts of Salvia polystachya. Polystachyne G (1) and 15-epi-polystachyne G (2) were isolated as an epimeric mixture, containing a 5-hydroxyfuran-2(5H)-one unit in the side chain at C-12 of...

Accurate DNA replication and repair are essential tasks for survival. In eukaryotes, DNA polymerases replicate genomes that can be composed of billions of base pairs. These genomes can be chemically damaged or modified, jeopardizing its integrity, and cells have evolved mechanisms to ameliorate the mutagenic effect of DNA damage. DNA replication an...

A novel begomovirus isolated from a Sida rhombifolia plant collected in Sinaloa, Mexico, was characterized. The genomic components of sida mosaic Sinaloa virus (SiMSinV) shared highest sequence identity with DNA-A and DNA-B components of chino del tomate virus (CdTV), suggesting a vertical evolutionary relationship between these viruses. However, r...

Entamoeba histolytica encodes four family B2 DNA polymerases that vary in amino acid length from 813 to 1279. These DNA polymerases contain a N-terminal domain with no homology to other proteins and a C-terminal domain with high amino acid identity to archetypical family B2 DNA polymerases. A phylogenetic analysis indicates that these family B2 DNA...

Amino acid sequence alignment of RB69, φ29 DNA polymerase and EhDNApolB2. Amino acid sequences were aligned using ClustalW. The conserved motifs in the exonuclease domains are indicated as ExoI, ExoII and ExoIII whereas the conserved motifs in the polymerase domain are indicated as A, B, and C. The YxGG/A motif involved in terminal protein interact...

Inhibition of EhDNApolB2 by aphidicolin. Percentage of DNA elongation activity of EhDNApolB2 using a γ-P32 17mer primer annealead to a circular ssDNA M13mp18 substrate in the presence of increasing aphidicolin concentrations. Reactions contained 20 nM of purified EhDNApolB2, 1 nM of circular substrate and increasing concentration of aphidicolin (0...

Entamoeba histolytica family B2 DNA polymerases. (DOC)

Oligonucleotides used for cloning and mutagenesis. (DOC)

Oligonucleotides used in primer extension and exonuclease reactions. (DOC)

Phylogenetic analysis and Modular organization family B2 DNA polymerases. (A) Phylogenetic analysis of the four family B2 DNA polymerases present in E. histolytica in relation to family B2 DNA polymerase from other protozoa, bacteriophages, and other eukaryotes. Accession numbers are indicated in Table S1. (B) Modular organization of family B2 DNA...

Exonuclease and polymerization activities of EhDNApolB2 and ΔTPR2. Reactions for panels A and B were carried out using a radiolabeled primer annealed to a complementary template as indicated in material and methods for EhDNApolB2 and ΔTPR2 in the presence (A) and absence (B) of dNTPs. (A)Autoradiogram showing the reaction products over a time cours...

Genbank identifiers of family B2 DNA polymerases. (DOC)

This Word file contains tables listing the strains and plasmids used in this study, as well as the sequence of oligonucleotides and probes used in gel shift assays.

In this Power Point file we show the results of gel shift assays with the protein extracts of P. syringae pv. phaseolicola NPS3121 grown at 28°C and 18°C, as well as the supershift assays using unrelated antibodies, including anti-His, anti-GST, and anti Rlk. Furthermore, we show the gel competition assays using the algD promoter as competitor. Det...

Pseudomonas syringae pv. phaseolicola, the causal agent of halo blight disease in beans, produces a toxin known as phaseolotoxin, in whose synthesis participate a group of genes organized within the genome in a region known as the "Pht cluster". This region, which is thought to have been acquired by horizontal gene transfer, includes 5 transcriptio...

DNA ligases play an essential role in DNA replication and repair. Herein, we report the cloning and biochemical characterization of DNA ligase I from the protozoan parasite Entamoeba histolytica (EhDNAligI). EhDNAligI is an ATP-dependent DNA ligase of 685 amino acids with 35% identity to human DNA ligase I. This report shows that heterologous expre...

GenBank accession numbers of the DNA polymerases used in the phylogenetic tree. This table contains the GenBank accession numbers of the DNA polymerases used to build the phylogenetic tree. (0.07 MB DOC)

mRNA expression profiles of the EhDNApolA gene. RT-PCR analysis of EhDNApolA (Upper panel) in comparison to actin (lower panel). Amplification products using isolated RNA treated with Reverse Transcriptase (RT +) (lanes 1 to 3) or with-out Reverse Transcriptase (RT -) (data not shown) were run on a 1% agarose gel and stained with ethidium bromide....

Putative DNA polymerases present in the genome of E. histolytica. This table presents a comparison between the putative DNA polymerases present in the genome of E. histolytica and homologous DNA polymerases. (0.05 MB DOC)

Kinetic parameters for nucleotide incorporation by EhDNApolA. This table contains the kinetics parameters (Km and Vmax) for nucleoide incorporation of EhDNApolA. (0.03 MB DOC)

Optimal activity conditions of EhDNApolA. To determine the optimal conditions for DNA polymerization, 60 fmol of EhDNApolA were incubated with 200 fmol of nicked substrate for 10 min under varying experimental conditions. DNA polymerase activity was measured by the extension of a 24 mer primer to a 45mer product (A) Effect of salt on DNA polymeriza...

Eukaryotic family A DNA polymerases are involved in mitochondrial DNA replication or translesion DNA synthesis. Here, we present evidence that the sole family A DNA polymerase from the parasite protozoan E. histolytica (EhDNApolA) localizes to the nucleus and that its biochemical properties indicate that this DNA polymerase may be involved in trans...