Guillermo Sebastian Natale

Guillermo Sebastian Natale
National Scientific and Technical Research Council | conicet · Centro de Investigaciones del Medio Ambiente (CIMA), Departamento de Quimica, Facultad de Ciencias Exactas, UNLP

PhD

About

61
Publications
19,500
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1,487
Citations
Citations since 2016
25 Research Items
1108 Citations
2016201720182019202020212022050100150200250
2016201720182019202020212022050100150200250
2016201720182019202020212022050100150200250
2016201720182019202020212022050100150200250

Publications

Publications (61)
Article
Full-text available
Imazethapyr is an herbicide that is used in a variety of crops worldwide, including soybean and corn. The aim of the present study was to evaluate the biomarkers responses of adult Leptodactylus latinasus exposed to the formulation Pivot® H (10.59% imazethapyr) in the laboratory at concentrations and under conditions that simulate two potential fie...
Preprint
Full-text available
Large-scale ecotoxicological studies have technical and ethical limitations, both related to the need to expose individuals to potentially harmful compounds. Computational modeling is a complementary useful and predictive tool that overcomes these limitations. Considering the increasing interest in the effects of pesticides on behavioral traits, th...
Preprint
Full-text available
In recent years, polybrominated diphenyl ethers or PBDE have been identified as the new emerging pollutants. These pollutants are derived from e-waste and their adverse effect on biota has been proven. In this work, the adverse effects of BDE-209 on mosquitofish ( Gambusia affinis) were evaluated. Acute toxicity bioassays were carried out with dail...
Article
Full-text available
This paper presents the first acute toxicity data of the natural insecticide spinosad in amphibians. The sensitivity of two neotropical sympatric anuran species, Boana pulchella and Rhinella arenarum, to spinosad-based formulation Tracer™ was evaluated. Lethal effects are reported in tadpoles of B. pulchella stage 25 between 2.81 and 35.44 mg spino...
Preprint
Full-text available
Imazetapir is a herbicide used in soybean and corn crops worldwide. Ecotoxicological studies have shown that imazetapir promotes genotoxic, biochemical and individual effects in aquatic vertebrates. In this study, we evaluated the response of different biomarkers in adult specimens of Leptodactylus latinasus exposed under laboratory conditions to t...
Article
Full-text available
In the context of the global amphibian crisis, biomonitoring constitutes a valuable assessment tool to provide critical up to date information on the status and health of amphibians worldwide. The aim of the current study was to evaluate the possible confounding effects of sex, size, and time since capture on enzymatic biomarkers and hematologic pa...
Article
We report sublethal effects of environmentally relevant concentrations of chlorpyrifos (CPF) at the individual (swimming alterations) and biochemical level (esterases activities and antioxidant enzymes) in the Montevideo treefrog Boana pulchella larvae. The LC50‐96h for CPF in stage‐29 B. pulchella tadpoles was 0.98 mg/L and was close to the 65th‐p...
Article
Horned Frogs of the family Ceratophryidae are conspicuous anurans represented by three endemic South American genera. Most ceratophryids inhabit semiarid environments, but three species of Ceratophrys occupy tropical or temperate humid areas. Several morphological and behavioral characters of larvae and adults are conserved across the family. Based...
Article
Full-text available
In September 2005, the IUCN Species Survival Commission (SSC) held a summit for the conservation of amphibians where specialists from around the world agreed that, in addition to the need to document the declines and extinctions, we need to promote action strategies designed to respond to the global biodiversity crisis. The outcome of this summit w...
Poster
Full-text available
Existen ambientes someros ubicados dentro de la traza urbana considerados como lagunas o humedales, que generalmente suelen estar afectados en distinto grado por estresores ambientales. Entre las herramientas para evaluar la calidad de estos ambientes, se encuentran los índices de integridad biótica (IBI). El presente trabajo tuvo como objetivo eva...
Article
Full-text available
Predation of eggs and embryos is one reason for early mortality in anurans, and it is known that embryos of several amphibian species can accelerate their hatching as a mechanism to reduce predation risk. Several authors have documented that hirudinids of several genera like Batracobdella, Desserobdella, Erpobdella, Haemopis, Hirudo, Macrobdella, P...
Article
Previous studies reported that some species of the family Ceratophryidae are able to produce sounds during premetamorphic tadpole stages. We have now determined the effects of the cholinesterase-inhibiting insecticide chlorpyrifos (CPF) on sounds emitted by tadpoles of Ceratophrys ornata. Tadpoles were exposed individually in order to evaluate the...
Article
In the last years, the agricultural expansion has led to an increased use of pesticides, with glyphosate as the most widely used worldwide. This is also the situation in Argentina, where glyphosate formulations are the most commercialized herbicides. It is known that glyphosate formulations are much more toxic than the active ingredient, and this d...
Article
In the present study, the damage recovery capabilities of Boana pulchella tadpoles after acute exposure (96 h) to 0.39 mg/L concentration of the imazethapyr (IMZT)-based herbicide formulation Pivot ® H (25% IMZT LC 50 value) were assessed during a period of 7 to −21 days. To appraise the recovery capabilities, frequency of micronuclei (MNs), other...
Article
Keywords: Insecticide Lethality Micronuclei Multi effect approach Sublethal endpoints A B S T R A C T Acute lethal and sublethal toxicity of the pirimicarb-based commercial formulation Aficida ® were evaluated on Boana pulchella tadpoles. Whereas mortality was used as end point for lethality, frequency of micronuclei and other nuclear abnormalities...
Article
Keywords: Endo III Fpg Herbicide Imazethapyr-based formulation Modified comet assay Oxidative DNA damage A B S T R A C T Imazethapyr (IMZT) is a selective postemergent herbicide with residual action. Available data analyzing its effects in aquatic vertebrates are scarce. In previous studies, we demonstrated that IMZT induces lesions into the DNA of...
Article
The chytrid fungus Batrachochytrium dendrobatidis (Bd) is one of the most important contributors for the decline of amphibian populations worldwide. Evidence indicates that the harmfulness of Bd infection depends on the species and life stage, the fungus strain, the season and environmental factors. In the present paper, we experimentally investiga...
Article
Full-text available
We evaluated the acute lethal and sublethal effects of technical-grade glyphosate (GLY) and the GLY-based commercial formulation Roundup ULTRA MAX® (RU) on two Gosner stages (Gss) 25 and 36 of the South-American Creole frog, Leptodactylus latrans. Bioassays were performed following standardized methods within a wide range of concentrations (0.0007-...
Presentation
Full-text available
La expansión e intensificación agrícola ha generado un aumento en el uso de agroquímicos que alcanzan diferentes ambientes naturales por deriva y escurrimiento superficial. La contaminación de ambientes acuáticos por agroquímicos genera impactos negativos sobre individuos, poblaciones y comunidades de organismos benéficos para la agricultura, pudie...
Article
Full-text available
Glyphosate (GLY) is the most used herbicide worldwide and its effects on anurans are well known. Pollutants can cause physiological and morphological effects. Therefore, this study evaluated the effects of GLY on hepatic melanomacrophages as a response to environmental stressors. Three treatments were exposed to different concentrations of pure GLY...
Article
Full-text available
Acute lethal and sublethal toxicity of the imidazolinone imazethapyr (IMZT)-based commercial formulation herbicide Pivot H® (10.59% IMZT) was evaluated on Hypsiboas pulchellus tadpoles. Whereas mortality was used as the end point for lethality, frequency of micronuclei (MNs) and other nuclear abnormalities as well as DNA single-strand breaks evalua...
Article
Full-text available
Previous studies found that two larvae of the family Ceratophryidae, Ceratophrys ornata (Common Name) and Ceratophrys cranwelli (Common Name), are able to produce underwater sounds as an antipredator strategy. Here, we determined whether tadpoles of another ceratophryid, Lepidobatrachus llanensis (Common Name), also produce underwater sounds in sim...
Article
Full-text available
We report the emission of underwater sounds in the tadpoles of a second member of the family Ceratophryidae, Ceratophrys cranwelli. These tadpoles produce a short metallic-like sound, which consists of short trains of pulses at Gosner stages 25, 28, and 37. Experiment I was designed to record underwater sounds and their characteristics. Experiment...
Article
The neonicotinoid insecticide imidacloprid (IMI) affects the insect central nervous system and is successfully applied to control pests for a variety of agricultural crops. In the current study, acute toxicity and genotoxicity of the IMI-containing commercial formulation insecticide Glacoxan Imida (35 percent IMI) was evaluated on Hypsiboas pulchel...
Article
Full-text available
The occurrence of abnormalities in amphibians has been reported in many populations, and its increase could be related to environmental pollution and habitat degradation. We evaluated the type and prevalence of abnormalities in 5 amphibian populations from agroecosystems with different degrees of agricultural disturbance (cultivated and reference a...
Article
Full-text available
A más de una década de la primera Lista Roja de herpetofauna propuesta por la Asociación Herpetológica Argentina (Lavilla et al., 2000), se recategorizaron los anfibios a partir de nueva información taxonómica, biogeográfica, bio-ecológica y de modificaciones metodológicas respecto a la evaluación anterior. Mediante la participación de 35 especiali...
Article
Full-text available
Effects of the widely employed insecticide Lorsban(®)48E formulation of chlorpyrifos (CPF) was studied on Rhinella fernandezae tadpoles, a native species of Argentina, Brazil, Paraguay and Uruguay, under the hypothesis of a differential response of organisms from ponds of two sites with different degree of anthropogenic disturbance: S1 an unpollute...
Article
Full-text available
Revisamos el método utilizado para determinar especies amenazadas en la Argentina, con los objetivos de: (1) que pueda ser empleado consistentemente por diferentes personas reduciendo la subjetividad, (2) brindar una guía sobre cómo evaluar los factores que conducen al riesgo de extinción, (3) proporcionar a los usuarios de listas rojas una mejor c...
Article
Full-text available
More than a decade after the first red list of herpetofauna proposed by the Asociación Herpetológica Argentina (Lavilla et al., 2000), we recategorized amphibians based on new taxonomic, biogeographical, bio-ecological information and methodological changes in the former evaluation. Through the participation of 35 specialists from all over Argentin...
Article
Full-text available
Parameters indicative of general condition and health were compared amongst adult frogs inhabiting uncultivated lands and fields subjected to agricultural practices typical of the humid pampas of Argentina. Whereas no significant differences existed in the parasite load and external malformations prevalence rate of adult frogs from either environme...
Article
Full-text available
Within the last two decades Batrachochytrium dendrobatidis (B.d.), the causative agent of chytridiomycosis, seems to have become a pandemic parasite, and is proposed as one of the more important causes of amphibian declines worldwide. In Argentina, the first report was in 2002 in Leptodactylus ocellatus. Since then, the fungus has expanded through...
Article
Full-text available
First citation for the county and the southernmost record known for the frog Scinax granulatus. This is the first documentation of this frog inhabiting mountain environments at 170 m elev. Present record extends the distribution of this frog 120 km to the SW from nearest known locality.
Article
Full-text available
The study of the effects of the insecticide cypermethrin (CY) technical grade and its Sherpa® commercial formulation on Hypsiboas pulchellus tadpoles assessing lethality, behavior, growth, and abnormalities under standardized laboratory conditions is reported. Observed behaviors were identified and categorized by means of a ranking system according...
Article
Full-text available
It is well known that many anurans do not reproduce easily in captivity. Some methods are based on administration of mammalian hormones such as human chorionic gonadotropin, which are not effective in many frogs. There is a need for simple, cost-effective alternative techniques to induce spawning. Our new method is based on the injection of a combi...
Data
Full-text available
Supplemental figure 1:> Froglets and tadpoles resulting from induced spawning using the AMPHIPLEX method. Shown are photographs of Lithobates pipiens, Ceratophrys ornata, Odontophrynus americanus and Ceratophys cranwelli.
Article
Full-text available
Natale, G.S., Alcalde, L., Herrera, R., Cajade, R., Schaefer, E.F., Marangoni, F. and Trudeau, V.L. 2011. Underwater acoustic communication in the macrophagic carnivorous larvae of Ceratophrys ornata (Anura: Ceratophryidae). —Acta Zoologica (Stockholm) 92: 46–53. We provide the first evidence for sound production by anuran larvae. In this study, we...
Article
Acute toxicity, genotoxicity, and cytotoxicity of the pirimicarb-containing commercial-formulation carbamate insecticide Aficida(R) (50% pirimicarb) were evaluated on Rhinella arenarum (Anura, Bufonidae) tadpoles exposed under laboratory conditions. Lethal and sublethal effects were employed as bioassays for acute toxicity, whereas micronuclei (MNi...
Article
Full-text available
This study presents results at a local scale of the impact of pesticide aerial applications containing a mixture of endosulphan and cypermethrin on anurans assessed by means of field evaluations. Additionally, effects were compared with laboratory toxicity tests in standardised conditions. Field assessment with native population allowed the detecti...
Chapter
Full-text available
In the last decades Argentine farmers have massively adopted the genetically modified glyphosate resistant soybean together with no tillage managerial practices. The area cultured with soybeans increased to 15 million ha with consequent higher pesticide consumption. Although recent publications deal with some detailed aspects of pesticide effects o...
Article
Full-text available
Disappearance of floating macrophytes was observed in two successively constructed wetlands for the treatment of wastewater that contained Zn, Ni and Cr at a tool factory in Argentina. Experimental work was developed to test the hypothesis that nutrient enrichment enhances the metal tolerance of floating macrophytes. Relative growth rates, root bio...
Article
Full-text available
In the last few years there has been great concern about declines in the abundance of several species of amphibians around the world. Among amphibians, anurans have a biphasic life cycle, with aquatic tadpoles and generally terrestrial adults, and they have an extremely permeable skin, making them excellent indicators of the health of the environme...
Article
Full-text available
This paper describes the external and buccopharyngeal morphology, chondrocranium and cranial muscles in tadpoles of Physalaemus fernandezae. The data are compared with those for other species of Physalaemus to improve the diagnosis of the "species group" within the genus. Species of the "P. biligonigerus" group have four infralabial papillae, two s...
Article
Full-text available
The present contribution is the first report of parasitosis by a chytrid fungus in wild anuran amphibians in Argentina, as well as the first case of amphibian mortality documented to date in Argentina. We report the presence of the chytrid fungus in dead adult Leptodactylus ocellatus. It has been suggested that chytridiomycosis is the main cause of...
Article
Hylidae are one of the most diverse and widely distributed frog families in the world. The species selected in the present study is representative for the subtropical wetland area of South America. Considering the global decline of amphibian populations, the present contribution is aimed towards the sensitivity of three frog species to a common pol...

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