Guillermo Lopez-Lluch

Guillermo Lopez-Lluch
Universidad Pablo de Olavide | UPO · Department of Physiology, Anatomy and Cellular Biology

Ph. Dr.

About

146
Publications
24,897
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Introduction
Metabolism is important in aging process. Our current research is focus on the study of oxidative metabolism and antioxidant activities along aging and their regulation by nutrition, bioactive compounds and physical activity.
Additional affiliations
May 2003 - present
Universidad Pablo de Olavide
Position
  • Professor (Full)
June 1999 - October 2000
University College London
Position
  • PostDoc Position
January 1999 - present
Universidad Pablo de Olavide
Position
  • Research Associate
Description
  • Mixed center for research funded by CSIC, University of Pablo de Olavide and Andalusian Government.
Education
September 1992 - July 1997
University of Cordoba (Spain)
Field of study
  • Biology
June 1987 - July 1992
University of Cordoba (Spain)
Field of study
  • Biology

Publications

Publications (146)
Article
Brain deterioration with age is associated with inflammation and oxidative stress that result in structural and functional changes. Recent studies have indicated that coenzyme Q10 (CoQ10) is associated to neurological oxidative stress and cognitive impairment. Studies with older people have shown a relationship between neurodegenerative diseases an...
Article
Full-text available
ADCK2 haploinsufficiency-mediated mitochondrial coenzyme Q deficiency in skeletal muscle causes mitochondrial myopathy associated with defects in beta-oxidation of fatty acids, aged-matched metabolic reprogramming, and defective physical performance. Calorie restriction has proven to increase lifespan and delay the onset of chronic diseases associa...
Article
Full-text available
Objectives The use of whole body-electrostimulation (WB-EMS) in combination with strength exercises has been proposed in order to improve muscle strength and rehabilitation. However, the combination of stimulation of muscle with exercise can induce muscle fibers break. This manuscript study Pilates Mat without or in combination with WB-EMS in train...
Article
Full-text available
Coenzyme Q10 (CoQ10) is an essential factor for mitochondrial activity and antioxidant protection of cells, tissues and plasma lipoproteins. Its deficiency has been associated with aging progression in animals and humans. To determine if CoQ10 levels in plasma can be associated with frailty in elderly people (aged > 65), we studied the relationship...
Chapter
Coenzyme Q (CoQ) is a ubiquitous lipidic molecule located in all cell membranes and lipoproteins in blood plasma. Its phenolic head gives it the capacity to accept and donate electrons. For this reason, it is the main lipidic redox compound in cells. This property permits CoQ to take part in the mitochondrial electron transfer chain and in many oth...
Article
Full-text available
Coenzyme Q is a unique lipidic molecule highly conserved in evolution and essential to maintaining aerobic metabolism. It is endogenously synthesized in all cells by a very complex pathway involving a group of nuclear genes that share high homology among species. This pathway is tightly regulated at transcription and translation, but also by enviro...
Article
Cardiovascular diseases are the leading cause of death globally and more than four out of five cases are due to ischemic events. Cardiac fibroblasts (CF) contribute to normal heart development and function, and produce the post‐ischemic scar. Here, we characterize the biochemical and functional aspects related to CF endurance to ischemia‐like condi...
Article
Full-text available
Primary coenzyme Q10 (CoQ) deficiency includes a heterogeneous group of mitochondrial diseases characterized by low mitochondrial levels of CoQ due to decreased endogenous biosynthesis rate. These diseases respond to CoQ treatment mainly at the early stages of the disease. The advances in the next generation sequencing (NGS) as whole-exome sequenci...
Article
Full-text available
Resumen Introducción La vitamina D y los polimorfismos en el receptor de vitamina D (VDR) se asocian con enfermedades autoinmunes, incluido el lupus eritematoso sistémico (LES). El objetivo de este estudio es analizar la asociación genética entre los polimorfismos de VDR (TaqI, ApaI, BsmI y FokI) y la susceptibilidad al LES, así como su relación c...
Article
Coenzyme Q10 (CoQ10 ) deficiency is a rare disease characterized by a decreased accumulation of CoQ10 in cell membranes. Considering that CoQ10 synthesis and most of its functions are carried out in mitochondria, CoQ10 deficiency cases are usually considered a mitochondrial disease. A relevant feature of CoQ10 deficiency is that it is the only mito...
Article
Coenzyme Q (CoQ) is a key component for many essential metabolic and antioxidant activities in cells in mitochondria and cell membranes. Mitochondrial dysfunction is one of the hallmarks of aging and age-related diseases. Deprivation of CoQ during aging can be the cause or the consequence of this mitochondrial dysfunction. In any case, it seems cle...
Article
SARS-CoV-2 causes a severe pneumonia (COVID-19) that affects essentially elderly people. In COVID-19, macrophage infiltration into the lung causes a rapid and intense cytokine storm leading finally to a multi-organ failure and death. Comorbidities such as metabolic syndrome, obesity, type 2 diabetes, lung and cardiovascular diseases, all of them ag...
Article
Full-text available
Non-alcoholic fatty liver disease (NAFLD) is a major health problem, and its prevalence has increased in recent years. Diet and exercise interventions are the first-line treatment options, with weight loss via a hypocaloric diet being the most important therapeutic target in NAFLD. However, most NAFLD patients are not able to achieve such weight lo...
Article
Full-text available
The apoptotic nuclease EndoG is involved in mitochondrial DNA replication. Previous results suggested that, in addition to regulate cardiomyocyte hypertrophy, EndoG could be involved in cell proliferation. Here, by using in vivo and cell culture models, we investigated the role of EndoG in cell proliferation. Genetic deletion of Endog both in vivo...
Chapter
Organisms maintain a complex relationship between the production of oxidative radicals and endogenous antioxidant levels and antioxidant enzymatic activities. Apart of its bioenergetics role in mitochondria, CoQ10 is also present in the rest of cell membranes and in plasma lipoproteins. In these structures, CoQ10 plays a key antioxidant role, preve...
Chapter
The regulation of gene expression needs many mechanisms of control which are multiple and diverse, encompassing from DNA structure to synthesis, modification and location of proteins. These processes, in addition to being tissue-specific, change during embryonic development and throughout the life of the organism. CoQ10 is essential to maintain cel...
Chapter
The decline of the immune system associated to aging is becoming a global issue nowadays due to the increase of lifespan. Immunosenescence is the deteriorated adaptative capacity of the immune system leading to increase in morbidity and mortality in elderly population. Aging has been related to a progressive affectation of mitochondria such as incr...
Article
Full-text available
Ubiquinol, the reduced form of Coenzyme Q 10 (CoQ 10 ), is a key factor in bioenergetics and antioxidant protection. During competition, professional soccer players suffer from considerable physical stress causing high risk of muscle damage. For athletes, supplementation with several antioxidants, including CoQ 10 , is widely recommended to avoid o...
Book
This book offers a comprehensive exploration of research on the essential relationship of the coenzyme Q10 and the process of aging in living organisms. CoQ10 is an important factor in two main aspects of cell physiology: bioenergetics and antioxidant protection. While primary deficiency of CoQ10 is associated with severe and lethal disease, second...
Article
Full-text available
Resveratrol (RSV) is a bioactive natural molecule that induces antioxidant activity and increases protection against oxidative damage. RSV could be used to mitigate damages associated to metabolic diseases and aging. Particularly, RSV regulates different aspects of mitochondrial metabolism. However, no information is available about the effects of...
Chapter
Coenzyme Q10 (CoQ10) is an essential factor for aerobic bioenergetics and antioxidant protection in cell membranes and blood plasma lipoproteins. It is a component of the electron transport chain in mitochondria and the trans plasma membrane electron transport. Its deficiency in human causes CoQ10 deficiency syndrome, a heterogeneous group of mitoc...
Article
Full-text available
Coenzyme Q10 (CoQ10) is an essential lipid present in all cell membranes[...]
Article
Full-text available
Fatty acids and glucose are the main bioenergetic substrates in mammals. Impairment of mitochondrial fatty acid oxidation causes mitochondrial myopathy leading to decreased physical performance. Here, we report that haploinsufficiency of ADCK2, a member of the aarF domain-containing mitochondrial protein kinase family, in human is associated with l...
Preprint
Full-text available
Fatty acids and glucose are the main bioenergetic substrates in mammals that are alternatively used during the transition between fasting and feeding. Impairment of mitochondrial fatty acid oxidation causes mitochondrial myopathy leading to decreased physical performance. Here, we report that haploinsufficiency of ADCK2, a member of the aarF domain...
Chapter
Calorie restriction is the most effective intervention with prolongevity effects in many of the organisms. Calorie restriction can be considered as the reduction of the intake of energy without reaching malnutrition. The effect of calorie restriction is not only reserved to an extended longevity but also delays the onset and reduces the progression...
Article
Full-text available
RESUMEN Se hace un estudio sobre la evaluación de los gestos técnicos desarrollados en un partido de goalball de varios equipos, ya que son pocos los estudios realizados en grupos de personas con deficiencia sensorial visual (DSV) hasta el momento y es necesario tener una mayor literatura especializada sobre este grupo específico. La práctica de la...
Article
The mitochondrial intramembrane rhomboid protease PARL has been implicated in diverse functions in vitro, but its physiological role in vivo remains unclear. Here we show that Parl ablation in mouse causes a necrotizing encephalomyelopathy similar to Leigh syndrome, a mitochondrial disease characterized by disrupted energy production. Mice with con...
Article
Full-text available
Mitochondria are key in the metabolism of aerobic organisms and in ageing progression and age-related diseases. Mitochondria are essential for obtaining ATP from glucose and fatty acids but also in many other essential functions in cells including aminoacids metabolism, pyridine synthesis, phospholipid modifications and calcium regulation. On the o...
Article
Full-text available
Abstract: Currently very low amount of scientific studies analyse the effect of physical activity on physiological and psychological parameters on people suffering visual sensorial deficiencies and blind. These persons form a particular neglected population and the information about how physical activity affects their quality of life related to hea...
Article
Loss of skeletal muscle mass and function is a hallmark of aging. This phenomenon has been related to a dysregulation of mitochondrial function and proteostasis. Calorie restriction (CR) has been demonstrated to delay aging and preserve function until late in life, particularly in muscle. Recently, we reported the type of dietary fat plays an impor...
Preprint
Full-text available
The mitochondrial intramembrane rhomboid protease Parl has been implicated in diverse functions in vitro, but its physiological role in vivo remains unclear. Here we show that Parl ablation in mouse causes a striking necrotizing encephalomyelopathy similar to Leigh syndrome, a mitochondrial disease characterized by disrupted energy production. Mice...
Article
Full-text available
This study aimed to compare the effect of high-intensity interval training (HIIT) with additional whole-body vibration (WBV) on body composition and lipid profile in obese/overweight adults on a hypocaloric diet. Forty adults were randomly assigned to (a) HIIT and vibration and hypocaloric diet (HIITWBV, n = 13), (b) HIIT and diet (HIIT, n = 14), a...
Article
Objectives: Bioavailability of supplements with coenzyme Q10 (CoQ10) in humans seems to depend on the excipients of formulations and on physiological characteristics of the individuals. The aim of this study was to determine which factors presented in CoQ10 supplements affect the different response to CoQ10 in humans. Methods: We tested seven di...
Article
SOX2 (Sex-determining region Y box 2) is a transcription factor expressed in several foetal and adult tissues and its deregulated activity has been linked to chronic diseases associated with ageing. Nevertheless, the level of SOX2 expression in aged individuals at the tissue level has not previously been examined. In this work, we show that SOX2 ex...
Article
Full-text available
Coenzyme Q (CoQ) is an essential component of the mitochondrial electron transport chain and an antioxidant in plasma membranes and lipoproteins. It is endogenously produced in all cells by a highly regulated pathway that involves a mitochondrial multiprotein complex. Defects in either the structural and/or regulatory components of CoQ complex or i...
Conference Paper
El goalball es un deporte específico para personas con discapacidad sensorial visual (DSV). A pesar de su antigüedad como deporte paralímpico (1976), en la actualidad encontramos pocos estudios científicos que analizan las características morfuncionales y de condición física de los jugadores. Por lo tanto, esta investigación se plantea como un apor...
Conference Paper
Full-text available
Dentro de las evaluaciones imprescindibles del deportista se encuentra la evaluación morfofuncional, la cual nos permite controlar variables relacionadas con la nutrición y el entrenamiento a fin de optimizar su rendimiento durante los entrenamientos y competencias. El objetivo principal de este estudio ha sido realizar un análisis cineantropométri...
Poster
Full-text available
Hacemos un estudio sobre la evaluación de los gestos técnicos desarrollados en un partido de goalball de varios equipos, ya que son pocos los estudios realizados en grupos de personas con deficiencia sensorial visual (DSV) hasta el momento y es necesario tener una mayor literatura especializada sobre este grupo específico. La práctica de la activid...
Article
Introduction: Coenzyme Q is an essential component in the activity of the mitochondrial electron transport chain. Its synthesis involves, at least, a complex of ten different proteins. In this study, an attempt is made to determine the evolution of the expression of the genes involved in coenzyme Q synthesis during mouse ageing. Material and meth...
Article
Full-text available
Introduction: Systemic lupus erythematosus is an autoimmune disease in which the severity varies according to race, sex and age of onset. This variation is also observed in the genetic markers associated with the disease, including PTPN22, VDR and TNF genes. The genetic stratification in different populations worldwide can influence the variabilit...
Article
Objective: To determine the influence of the level of physical activity on physical fitness, body composition and lipid profile and further explore the associations between fitness with these health outcomes in a population of overweight/obese adults. Methods: Forty overweight/obesity participants with a caloric intake between a negative balance...
Chapter
Coenzyme Q is an essential factor in bioenergetics and in antioxidant protection of cell membranes. As the main antioxidant in blood, coenzyme Q prevents the oxidation of lipoproteins. This function and others related to the physiology of the heart and the vascular endothelium makes this molecule a key factor in preventing atherosclerosis and cardi...
Article
Mitochondria play an essential role in ageing and longevity. During ageing, a general deregulation of metabolism occurs, affecting molecular, cellular and physiological activities in the organism. Dysfunction of mitochondria has been associated with ageing and age-related diseases indicating their importance in the maintenance of cell homeostasis....
Article
Full-text available
Introducción. El lupus eritematoso sistémico es una enfermedad autoinmunitaria cuya gravedad varía según la raza, el sexo y la edad de aparición. Esta disparidad también se observa en los marcadores genéticos asociados con la enfermedad presentes en los genes PTPN22, VDR y TNF. La estratificación genética que presentan las diferentes poblaciones en...
Article
Decrease in muscle mass and performance with ageing is one of the main factors of frailty in the elderly. Maintenance of muscle performance by involving in physical activities is essential to increase independence and quality of life among elderly. The use of natural compounds with ergogenic activity in old people would increase the effect of moder...
Article
The importance of mitochondrial dynamics and mitophagy during muscle immobilization and remobilization is reported in this issue of Acta Physiologica by Kang et al. (Kang et al., 2016). In these processes the essential role of FoxO3 is highlighted. Activation of FoxO3 directs the activation of the autophagic/mitophagic pathway producing the fragmen...
Article
Full-text available
p>La Nefritis Lúpica (NL) es más frecuente en mujeres. Se reconoce que madres con Lupus Eritematoso Sistémico (LES) confieren mayor riesgo al desarrollo de esta entidad en sus hijos. Esta transmisión se debe a la impronta genómica y/o al genotipo materno sobre el desarrollo prenatal. En este estudio se identificaron variantes de los sistemas PTPN22...
Data
Fig. S3 Representative gels stained with Ponceau S used to normalize quantifications of the different antibody bands shown in this paper.
Data
Fig. S1 Cross‐section of a proximal convoluted tubule (PCT) from a six‐month old control animal.
Data
Fig. S4 Correlation analyses between different glomerular filtration structures (panels A, B and C) and glomerular structures versus mitochondrial mass in epithelial cells from proximal convoluted tubules (D, E and F). Panel A shows filtration slits (FS) versus glomerular basal membrane (GBM) thickness; panel B, podocyte foot processes (PFP) versus...
Data
Fig. S2 Representative images of cytoplasm portions of PCT epithelial cells from control (A) and 18‐months CR‐submitted animals with different dietary fats (B, CRL; C, CRS and D, CRF) showing a relatively large number of mitochondria (arrows). In C and D swollen mitochondria are clearly visible. The bars are equal to 2 µm (N = nucleus).
Article
Full-text available
Calorie restriction (CR) has been repeatedly shown to prevent cancer, diabetes, hypertension, and other age-related diseases in a wide range of animals, including non-human primates and humans. In rodents, CR also increases lifespan and is a powerful tool for studying the aging process. Recently, it has been reported in mice that dietary fat plays...
Article
Calorie restriction (CR) consistently extends longevity and delays age-related diseases across several animal models. We have previously shown that different dietary fat sources can modulate life span and mitochondrial ultrastructure, function and membrane fatty acid composition in mice maintained on a 40% CR. In particular, animals consuming lard...
Article
Full-text available
El proceso de formación de futuros deportistas debe ser un trabajo estructurado y planificado para poder alcanzar el máximo nivel deportivo. Es fundamental en este periodo tener presentes los ritmos de desarrollo y maduración de los jóvenes deportistas para así adecuar las cargas de entrenamientos a sus estados evolutivos. El objetivo del estudio f...
Article
Full-text available
Amine fungicides are widely used as crop protectants. Their success is believed to be related to their ability to inhibit postlanosterol sterol biosynthesis in fungi, in particular sterol‐Δ8,Δ7‐isomerases and sterol‐Δ14‐reductases, with a concomitant accumulation of toxic abnormal sterols. However, their actual cellular effects and mechanisms of de...
Article
Full-text available
The formation of future athletes must be structured and planned in order to achieve the highest performance level. It is necessary to take into account the rates of development and maturation of young athletes in this early period in order to adjust the training loads to their developmental stages. The aim of this study has been to analyze the morp...
Article
Full-text available
Coenzyme Q (CoQ) is a key component of the mitochondrial respiratory chain carrying electrons from complexes I and II to complex III and it is an intrinsic component of the respirasome. CoQ concentration is highly regulated in cells in order to adapt the metabolism of the cell to challenges of nutrient availability and stress stimuli. At least ten...
Article
Full-text available
Ageing causes loss of functions in tissues and organs, is accompanied by a chronic inflammatory process and affects life and health span. Calorie restriction (CR) is a non-genetic intervention that prevents age-associated diseases and extends longevity in most of the animal models studied so far. CR produces a pleiotropic effect and improves multip...
Article
Full-text available
Ageing is accompanied by the accumulation of damaged molecules in cells due to the injury produced by external and internal stressors. Among them, reactive oxygen species produced by cell metabolism, inflammation or other enzymatic processes are considered key factors. However, later research has demonstrated that a general mitochondrial dysfunctio...
Article
Full-text available
The Membrane Theory of Aging proposes that lifespan is inversely related to the level of unsaturation in membrane phospholipids. Calorie restriction (CR) without malnutrition extends lifespan in many model organisms, which may be related to alterations in membrane phospholipids fatty acids. During the last few years our research focused on studying...
Article
Full-text available
Background Oxidative stress has been considered one of the causes of aging. For this reason, treatments based on antioxidants or those capable of increasing endogenous antioxidant activity have been taken into consideration to delay aging or age-related disease progression. Aim In this paper, we determine if resveratrol and exercise have similar ef...
Article
Full-text available
Calorie restriction (CR) without malnutrition extends life span in several animal models. It has been proposed that a decrease in the amount of polyunsaturated fatty acids (PUFAs), and especially n-3 fatty acids, in membrane phospholipids may contribute to life span extension with CR. Phospholipid PUFAs are sensitive to dietary fatty acid compositi...
Article
The impact of aging and physical capacity on coenzyme Q10 (Q10) levels in human blood is unknown. Plasma Q10 is an important factor in cardiovascular diseases. To understand how physical activity in the elderly affects endogenous Q10 levels in blood plasma, we studied a cohort of healthy community-dwelling people. Volunteers were subjected to diffe...
Article
Full-text available
Imbalance between proliferation and cell death accounts for several age-linked diseases. Aging, calorie restriction (CR), and fat source are all factors that may influence apoptotic signaling in liver, an organ that plays a central metabolic role in the organism. Here, we have studied the combined effect of these factors on a number of apoptosis re...