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Guillaume H M Counotte

Guillaume H M Counotte
Cvier consultancy

PhD

About

68
Publications
21,515
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1,053
Citations
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January 2006 - May 2019
Gezondheidsdienst voor Dieren
Position
  • Senior Researcher

Publications

Publications (68)
Article
Full-text available
The aim of this project was to identify blood analytes which are associated with obesity and/or insulin dysregulation (ID) in horses. For this study, 57 horses were selected. At the start, body condition score (BCS) was established, and an oral sugar test (OST) was performed. Furthermore, the following blood analytes were determined: basal glucose,...
Article
Straightforward testing procedures to enable the diagnosis of insulin dysregulation (ID) in horses that are suitable for use in daily veterinary practice are needed because of the risk that ID could result in laminitis. In our study (that included 90 horses), we compared the proportion of horses classified as ID-positive, ID-suspect, and ID–not dia...
Article
Full-text available
Trace elements and potential toxic elements were analyzed in bovine livers submitted for autopsy in the Netherlands during the years 2007 to 2018. The age of each animal was recorded. In total, 1544 livers were analyzed for cadmium, cobalt, chromium, copper, iron, molybdenum, nickel, lead, selenium and zinc. Less than 2% of the liver samples were f...
Data
Linear regression results of elements with year and age. (DOCX)
Article
Full-text available
The influence of supplements containing magnesium on the aetiology of osteochondrosis is unknown. We did two studies to measure the effect of additional minerals (especially magnesium) on osteochondrosis. In study 1 (5 studs, in total 64 mares and foals aged 0 to 5 months, equally divided into two groups) supplementation with minerals and placebo w...
Article
Full-text available
Drinking water can be considered an essential nutrient for dairy cattle. However, because it comes from different sources, its chemical and microbiological quality does not always reach accepted standards. Moreover, water quality is not routinely assessed on dairy farms. The microecology of drinking water sources and distribution systems is rather...
Article
Full-text available
The study had two objectives: I, to measure levels of two bone-specific biomarkers, with a view to identifying sows with leg problems; 2, to evaluate the effect of additional vitamin D/ monocalcium phosphate on the two bone-specific biomarkers and the number of stillborn piglets. Of 272 third or higher parity sows, 136 were randomly selected to rec...
Article
Full-text available
Toxic silo gases are a potential danger to livestock housed in close proximity to roughage silos. These gases, such as nitrogen dioxide (NO2), may be produced during the early stages of (maize and grass) silage making. In humans, inhalation of these gases causes a condition known as 'Silo Filler's Disease' (SFD), which is a recognized occupational...
Article
Full-text available
Selenium is an essential part of the enzyme glutathione-peroxidase (GSH-Px) and plays an important role in the intracellular aspecific immune defence. Reference values for blood levels of GSH-Px are not available for dairy goats. The EU has authorized the addition of selenium (as E), in the form of sodium selenite or sodium selenate, to animal feed...
Article
Full-text available
Reference values have been established for serum biochemical parameters in sows from high producing pig herds. In total, 132 clinically healthy sows from eight breeding herds were serially sampled three weeks and one week before farrowing, and at one week and three weeks after farrowing. Fourteen serum biochemical parameters, focusing mainly on ene...
Article
Full-text available
The results of global proficiency testing schemes (PTS) for serological tests to detect antibodies against infectious bursal disease virus (IBDV) and Newcastle disease virus (NDV) in chicken serum, in which 125 and 120 laboratories, respectively, participated from Africa, Asia, Europe, Central and South America, were used to analyse the performance...
Article
Full-text available
Serum, plasma, or urine samples are usually used for the measurement of the trace elements copper; zinc, iron, selenium, because these samples are easy to obtain; however; these samples are not always appropriate. For example, it is not possible to measure molybdenum, the major antagonist of copper; in blood or urine. Therefore measurement of trace...
Article
Full-text available
Within a 1-year period, three calves from the same herd developed ataxia of the hind limbs and urinary incontinence at about 6 months of age. Signs progressed and the calves were slaughtered 1-8 months after the onset of signs. The calves belonged to a suckling beef herd of 35 cattle. Blood samples from 11 cattle of different ages were collected an...
Article
Full-text available
A pilot study of 10 Dutch dairy herds was performed to investigate possible causes of stillbirth/perinatal weak calf syndrome in heifers. Possible causes and advice for further investigations on farms affected by this syndrome are discussed based on the results of investigations of blood and urine from pregnant young stock, postmortem examination o...
Article
Stillbirth/perinatal weak calf syndrome in dairy heifers: results of a Pilot Study A pilot study of 10 Dutch dairy herds was performed to investigate possible causes of stillbirth/perinatal weak calf syndrome in heifers. Possible causes and advice for further investigations on farms affected by this syndrome are discussed based on the results of in...
Article
Full-text available
On a dairy farm situated 200 km from the sea on sandy soil an increasing problem arose during 3 years with new-born calves showing a thickness over the larynx. The calves and close-in heifers proved to be severely deficient in trace elements. The new born calves had goitre. The clinical picture, diagnosis and therapy/prevention are discussed.
Article
Full-text available
On a dairy farm situated 200 km from the sea on sandy soil an increasing problem arised during 3 years with new-born calves showing a thickness over the larynx. The calves and close-in heifers proved to be severely deficient in trace elements. The new born calves had goitre. The clinical picture, diagnosis and therapy/prevention arc discussed.
Article
Full-text available
Second-generation anticoagulant rodenticides can give rise to unexpected casualties in nontarget species in zoos. The first two offspring of a pair of turkey vultures (Cathartes aura) died of brodifacoum toxicosis. The adult birds fed rodenticide-killed mice to their offspring. There are previous case reports of small carnivorous birds (Dacelo nova...
Article
Zowel een tekort als een overmaat is schadelijk. Naast het kopergehalte in het rantsoen is de benutting een belangrijke factor.
Article
In dit rapport is de huidige kennis van de kopervoorziening van melkvee, met speciale aandacht voor het westelijk veenweidegebied, gebundeld. Praktische adviezen zijn opgesteld en kennishiaten zijn aangegeven.
Article
Death from fenitrothion intoxication in young broiler chicks has never been reported. The mortality in this instance was 16.4% in the first week. The dead birds were found lying in a ventral position resting their heads on the floor. Prior to death dyspnea and paralysis occurred. A concentration of 120 mg fenitrothion/kg of litter (dry weight) was...
Article
Full-text available
A study of the various types of statistical methods used for interlaboratory studies is presented. Where possible reference is made to current standards and guidelines in this area. From the discussions of these standards and practical experiences it became apparent that there is no ideal statistical method for interlaboratory studies in general. C...
Article
In 1989 an international veterinary sample exchange program for clinical chemistry and haematology was started. The program comprises 28 clinical chemical and haematological variables and was joined by 15 laboratories in Europe. Differences between laboratories and changes in repeatability (r); reproducibility (R), the coefficient of variation of t...
Article
Full-text available
The present study describes two experiments to standardize the phytohaemagglutinin (PHA) skin test as an indicator of lymphocyte reactivity in pigs after exposure to stressful situations in practical pig husbandry. The first experiment was a dose-response experiment. No time dose interaction was found, so the pattern of the reaction was the same fo...
Article
On a dairy farm a selenium deficiency was diagnosed on the basis of the blood chemistry of heifer calves. Of ten heifers, aged five to fourteen months, six animals had a glutathione peroxidase (GSH-PX) activity lower than 10 U/gHb. A good correlation was found between this low GSH-Px-activity and the selenium concentration in the blood. The animals...
Article
Full-text available
The data presented here were obtained using blood samples from 159 healthy, conventionally managed sows from 37 breeding herds. Sows were sampled at weaning and at 4-5 weeks gestation. Paired blood samples were analysed from sows that had a normal pregnancy and subsequently farrowed. The mean values of serum total protein, albumin and gamma globuli...
Article
Four experiments were conducted to test the hypothesis that the composition of cervical mucus can be used as an indicator of reproductive efficiency in the cow. In Experiment 1, biochemical changes were studied in cervical mucus during the estrous cycle. Sorbitol concentration was observed to be highest at 1 to 3 days prior to estrus and lowest on...
Article
Full-text available
The effect of feeding dairy cattle mangel-wurzels on the serum increased concentration of beta-hydroxybutyrate was investigated in serum is normally indicative of an increased catabolism of fats in the liver as a result of an energy shortage. Feeding cattle large amounts of mangel-wurzels can cause the serum beta-hydroxybutyrate concentration to in...
Article
Full-text available
In a cohort study, 40 pig finishing herds were selected: twenty pig herds with a low and twenty pig herds with a high prevalence of several pathological lesions recorded at slaughter in a six-month period before the start of the study. Blood samples were taken from 20 pigs per herd at the end of the finishing period to investigate haematological an...
Article
Full-text available
The determination of triclabendazole (Fasinex) in milk is described. The excretion of triclabendazole and its two main metabolites was measured under field conditions in twenty-five cows. Triclabendazole was administered to cows in a dosage of approximately 12 mg kg-1. The maximum concentration of the main metabolites of triclabendazole (triclabend...
Article
Full-text available
To identify a simple method for assessing the selenium demand in cattle, the relationship between selenium content in whole blood and the concentration of the selenium containing enzyme glutathione peroxidase (EC 1.11.1.9; GSH-Px) in red blood cells was studied. On six farms with suspected low soil selenium content, blood samples were collected fro...
Article
A metabolic profile test (MPT), consisting of the following parameters of the blood: glucose, urea, beta-hydroxybutyrate, copper, magnesium, calcium, non-esterified fatty acids, bilirubin, aspartate amino transferase, whole protein and the protein spectrum of the serum, was performed in twenty-two normal herds (control herds) in the Netherlands. Re...
Article
Three experimental studies were done on the effects of increased concentrations of nitrate in the drinking water of weaned piglets and flattening pigs throughout the weaning and fattening periods respectively on the experimental piggery in Raalte for pigs in the northern and eastern Netherlands. To begin with, prospective studies were done in three...
Article
A farm is described, on which the placenta was retained in approximately forty per cent of the cows and on which selenium deficiency (0.03 +/- 0.010 micrograms/ml) and unduly reduced activity of glutathione peroxidase (GSH-Px) in the erythrocytes (65.2 +/- 26.4 u/g Hb) were observed. When 0.5 mg of Se per kg of dry matter of a mixture of concentrat...
Article
A PVC ear tag containing the synthetic pyrethroid flucythrinate was tested in seventeen herds including 218 heifers. There was a comparable control group of twelve herds including 157 heifers. The ear tags were more effective against sucking flies than they were against sticking flies. Varying with the species of the fly, the effectiveness ranged f...
Article
The concentration of progesterone in milk was determined in 327 mares to establish pregnancy in an early stage (sixteen days or more). The 5 ng/ml-level of progesterone as a threshold in the EIA-system used resulted in a very useful aid in the diagnosis of early pregnancy in mares. The results of testing were not in accordance with the confirmed re...
Article
Serum lysozyme activity (SLA) was measured in a turbidimetric assay with a microcentrifugal analyzer. In a control group of 53 healthy dogs of both sexes and ranging in age from 4 to 10 years, SLA had a mean value of 1.2 mg/l with a range (+/- 2 SD) of 0.6 - 1.8 mg/l. In 80 dogs with a variety of neoplastic diseases the histopathological diagnosis...
Article
In a control group of fifty-three clinically healthy dogs of either sex and ranging in age from four to ten years, the serum lysozyme activity (SLA) showed a mean value of 1.2 mg/l with a range (+/- 2 SD) of 0.6-1.8 mg/l. SLA was measured in a turbidimetric assay using a microcentrifugal analyser. In dogs with a variety of neoplastic diseases, the...
Article
The distribution of oxytetracycline hydrochloride in two batches of 4,000 kg of pig-rearing pellets (400 ppm) was studied. The stability of oxytetracycline in this feed was also determined. The concentration of oxytetracycline in the feed was determined by high pressure liquid chromatography. The premix contained more oxytetracycline (27 per cent)...
Article
Full-text available
Concentrate ingredients (maize meal, coconut oilmeal, maize gluten feed, citrus pulp, tapioca and soya bean oilmeal) were given daily at 0900 h into the rumen of 4 cows for 5 successive days. Hay was given freely at 1300 h. Between experimental periods there were intervals of at least 17 days when hay was given at 0800 and 1700 h. In a second exper...
Article
Full-text available
The role of DL-lactic acid as an intermediate in the rumen of a Friesian X Holstein dairy cow adapted to a diet of hay ad libitum plus 12 kg of a concentrate mixture was studied in vitro and in vivo. Concentrations of soluble sugars in the rumen fluid became maximal at 30 min postfeeding, but at 90 min no sugars were detectable. The DL-lactate conc...
Article
2. Rumen fluid was sampled before feeding from cows given hay, diluted with an anaerobic salt solution and added (20 ml) to different amounts (mostly 1 g) of maize gluten meal, maize, citrus pulp, tapioca, beet pulp, coconut expeller or soya bean oilmeal for incubation at 39 deg C. After at least 4 h of incubation there were large differences in pH...
Article
Full-text available
The regulation of lactic acid production, the regulation of lactate fermentation and the role of lactate as intermediate in the rumen metabolism was studied. The pH had a pronounced effect on all three processes and therefore buffer capacity of the rumen contents is also described. Starch gave much less rise to lactic acidosis than soluble sugars,...
Article
Full-text available
1. Four cows with rumen cannulae were given 12 kg concentrates daily in 2 equal portions at 0800 and 1530, in one portion of 12 kg at 0800 or in 4 portions of 3 kg each at 0800, 1100, 1400 and 1700 h. Every period lasted 2 weeks. In addition to the concentrates hay was provided at 0900 and 1630 h and was adjusted so that the daily remainder was at...
Article
Full-text available
Since Megasphaera elsdenii ferments a variable part of dl-lactate to butyrate, measurement of the percentage of dl-lactate fermented to propionate via the acrylate pathway in rumen contents will underestimate the participation of M. elsdenii in the dl-lactate fermentation. The percentage of dl-[2-C]lactate fermented via the acrylate pathway and the...
Article
Full-text available
Without Abstract
Article
Full-text available
Tungstate prevented the formation of active nitrate reductase in growing rumen bacteria capable of nitrate reduction, but did not directly inhibit the enzyme activity of all strains tested.
Article
A number of kinetic parameters of the lactate dehydrogenases of three rumen bacterial species (Peptostreptococcus productus, Propionibacterium acnes and Actinomyces viscosus), the rumen ciliate Isotricha prostoma and mixed rumen microorganisms (MRM) with respect to NADH, pyruvate, fructose-1,6-diphosphate (FDP) as well as the effects of several nuc...
Article
Der Abbau von Aminosäuren und Peptiden durch gemischte Pansen-Mikroorganismen Es wurden kurzfristige Inkubationsversuche mit Pansen-Mikroorganismen und Zusatz von freien oder peptidgebundenen Aminosäuren durchgeführt. Es konnte gezeigt werden, daß aus der Netto-Geschwindigkeit der Fermentationsrate von freien Aminosäuren keine Aus-sage über die Ges...
Article
Full-text available
A method is presented for the analysis of buffer systems in the rumen using the first derivation of titration curves. Bicarbonate and volatile fatty acids (VFA) are the main components of the buffering system in the rumen fluid of dairy cattle under widely different feeding conditions. Phosphate from saliva is of little importance as a buffer, but...
Article
Full-text available
A simple method for the calculation of kinetic parameters (K(m), V(max)) under conditions of changing substrate concentrations is presented. An application of the method to detect shifts in groups involved in the utilization of a substrate in a mixed microbial culture is given.
Article
Schnell-Verfahren zur Bestimmung von mikrobiellen Mischkulturen aus dem Inhalt des Pansens, Caecums und aus Kot durch eine semi-quantitative Bestimmung einiger hydrolytischer Enzyme Ein Schnell-Verfahren zur semi-quantitativen Bestimmung der spezifischen Aktivitäten von 19 hydrolytischen Enzymen wurde angewendet, urn mikrobielle Mischkulturen aus P...
Article
Full-text available
This paper present recent results concerning both the role of lactate as an intermediate in rumen fermentation, and the rapid accumulation of lactic acid in ruminant lactic acidosis and related syndromes. Special attention is given to the factors which regulate microbial lactate formation and fermentation in the rumen.When readily fermentable carbo...

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Project (1)
Project
combining several blood parameters to an index for resilience in sport horses, swine and cattle.