Grigory G Martinovich

Grigory G Martinovich
Belarusian State University | BSU · Department of Biophysics

PhD., D.Sc.
Head of Biophysics Deparment

About

46
Publications
20,615
Reads
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280
Citations
Additional affiliations
June 2017 - present
Belarusian State University
Position
  • Head of Department
January 2015 - present
Belarusian State University
Position
  • Professor
Description
  • Lecture courses: Biochemistry; Cell biophysics; Physics of membrane systems; Bioenergetics and metabolism; Physics of solutions, Foundations of molecular and nanomedicine
September 2009 - January 2015
Belarusian State University
Position
  • Professor (Associate)
Description
  • Lecture courses: Biochemistry; Cell biophysics; Foundations of ecology; Physics of membrane systems; Bioenergetics and metabolism; Foundations of modern natural science
Education
September 1997 - September 1998
Belarusian State University
Field of study
  • Biophysics
September 1992 - July 1997
Belarusian State University
Field of study
  • Physics

Publications

Publications (46)
Book
Full-text available
В монографии обобщены современные представления о регуляторных механизмах в клетках, протекающих с участием молекулярного кислорода и его метаболитов. Детально рассмотрены структура и функционирование основных ферментативных систем, участвующих в продукции и метаболизме свободно-радикальных форм кислорода. Особое внимание уделено роли метаболитов к...
Article
Full-text available
Pharmacological modification of the redox properties of tumor cells is a promising approach to enhance the efficiency of antitumor therapy. The Nrf2 transcription factor is considered as a new target for the development of selective chemosensitizers. Nrf2 plays a key role in the regulation of cellular redox homeostasis against stress and during ada...
Article
Full-text available
Fluorescence assay and Raman spectroscopy were used to study the mechanisms of action of 2-isopropyl-5-methyl- 1,4-benzoquinone (thymoquinone) on HEp-2 human larynx carcinoma cells. Thymoquinone was found to have a more pronounced toxic effect than 1,4-benzoquinone and 2,3,5-trimethyl-1,4-benzoquinone. The action of thymoquinone leads to a decrease...
Article
The free-radical theory of aging, advanced more than 50 years ago by D.Harman, remains popular today. The review analyzes age-related changes in the main endogenous mechanisms of reactive oxygen species (ROS) production and antioxidant defense mechanisms. With age, ROS generation by mitochondria, peroxisomes, and NAD(P)H oxidases is enhanced, while...
Article
Full-text available
Autophagy is the main catabolic process required for the removal of damaged organelles, aggregated proteins and intracellular pathogens from cells. Oxidative stress is accompanied by an increase in autophagy, which has a protective effect by maintaining the qualitative composition of mitochondria (mitophagy) and peroxisomes (pexophagy) followed by...
Article
ROS are important intracellular messengers; their ambiguous role in malignant processes was demonstrated in many studies. The effects of a synthetic phenolic antioxidant sodium 3-(3’-tert-butyl-4’-hydroxyphenyl)propyl thiosulfonate sodium (TS-13) on the tumor growth and oncolytic properties of doxorubicin were studied in the experimental model of L...
Article
The effect of novel water-soluble structurally related monophenolic compounds on the activity of two most important mechanisms of maintaining intracellular homeostasis, autophagy and the redox-sensitive signal system Keap1/Nrf2/ARE, has been studied in human breast adenocarcinoma cell line MCF-7 using confocal microscopy. Autophagy processes were a...
Article
Reactive oxygen and nitrogen species are important intracellular messengers that are strongly implicated in tumor processes, yet their precise role in cancerogenesis is still largely unclear and often debatable. Plant and synthetic phenols and polyphenols are known to have antitumor effect through multiple mechanisms most importantly the one that a...
Article
Full-text available
The present research is devoted to the study of the relationship between the structure of the original synthetic monophenolic antioxidants and their ability to influence the activity of autophagy in tumor cells.
Conference Paper
Redox regulation of cell activity is the basis for antitumor therapy. In cancer cells generation of ROS is reduced, thus such cells have resistance to apoptotic signals. Malignant cells reproduce under hypoxic conditions and this fact reduces the probability of oxidative processes. Therefore, the use of oxidizing agents, such as quinones and their...
Article
Full-text available
The effect of ascorbate in physiological concentrations on the proliferative activity and chemoresistance in human larynx carcinoma HEp-2 cells was studied. Ascorbate in a concentration of 60 μM was found to increase the cancer cells proliferation rate 1.5 times. Ascorbate changes the functional state of the cancer cells, thereby increasing their r...
Article
Full-text available
Effects of water-soluble sulfur-containing phenolic antioxidants sodium 3-(3′-tert-butyl-4′- hydroxyphenyl)propyl thiosulfonate and potassium 3,5-dimethyl-4-hydroxybenzyl thioethanoate on chemoresistance in tumor cells have been studied. The studied phenolic antioxidants cause oppositely directed changes in the redox properties and chemoresistance...
Article
Full-text available
Nrf2 transcription factor plays a key role in maintaining cellular redox balance under stress and is a perspective target for oxidative stress-associated diseases. Under normal conditions, Nrf2 transcriptional activity is low due to its rapid ubiquitination and degradation in the 26S proteasome, as well as through various modifications of amino aci...
Book
В учебном пособии обобщены современные биофизические представления о механизмах преобразования энергии в живых системах. Детально рассмотрены структура и функционирование основных макромолекулярных преобразователей энергии в митохондриях и хлоропластах. Особое внимание уделено явлению, играющему исключительную роль в биоэнергетике и молекулярной би...
Article
Full-text available
Mechanisms of tumor-cell responses to 2-isopropyl-5-methyl-1,4-benzoquinone (thymoquinone) and 1,4-benzoquinone were studied using fluorescence and the inhibition assay. It was shown that quinones enhanced the intracellular production of reactive oxygen species, reduced the mitochondrial membrane potential, and induced tumor-cell death through diff...
Article
Full-text available
С применением методов флуоресцентного и ингибиторного анализа исследованы механизмы отклика опухолевых клеток на действие 2-изопропил-5-метил-1,4-бензохинона (тимохинона) и 1,4-бензохинона. Обнаружено, что хиноны усиливают внутриклеточную продукцию активных форм кислорода (АФК), вызывают снижение митохондриального мембранного потенциала и индуцирую...
Article
Full-text available
It is established that thymoquinone (2-isopropyl-5-methyl-1,4-benzoquinone) and 1,4-benzoquinone regulate the intracellular reactive oxygen species (ROS) production and induce the death of tumor cells by different mechanisms. It is shown that the toxic action of 1,4-benzoquinone is associated with the inhibition of electron transfer in the mitochon...
Article
Full-text available
Many plant phenols (stilbenes, curcumins, catechins, flavonoids, etc.) are effective antioxidants and protect cells during oxidative stress. Extensive clinical studies on the potential of phenolic compounds for treatment of cardiovascular, neurodegenerative, oncological, and inflammatory diseases are now being conducted. In addition to direct antio...
Article
Full-text available
It was established that the response of tumor cells to the action of the redox-active compound is regulated by the intracellular redox state quantitatively characterized by the effective redox potential and the redox buffer capacity. Phenolic antioxidant 3,5-dimethyl-4-hydroxybenzyl thioetanoate (BEP-11-K) was found to stimulate the proliferation i...
Article
Full-text available
Effects of water-soluble phenolic antioxidant sodium 3-(3'-tert-butyl-4'-hydroxyphenyl)-propyl thiosulfonate (TS-13), potassium 3,5-dimethyl-4-hydroxybenzyl thioethanoate (BEP-11-K), and potassium 3-(3',5'-di-tert-butyl-4'-hydroxyphenyl)-propionate (potassium phenosan) on the proliferative activity of tumor cells and the role of redox-dependent and...
Article
Full-text available
Исследовано влияние водорастворимых фенольных антиоксидантов 3-(3'-трет-бутил-4'-гидроксифенил)-пропилтиосульфонат натрия (ТС-13), 3,5-диметил-4-гидроксибензилтиоэтаноат калия (БЭК-11-К) и 3-(3',5'-ди-трет-бутил-4'-гидроксифенил)-пропионат калия (фенозан-калий) на пролиферативную активность опухолевых клеток и роль редокс-зависимых и кальций-зависи...
Article
Full-text available
В обзоре рассмотрены современные представления об участии активных форм кислорода в регуляции функций биологических систем. Детально проанализированы современные подходы к описанию механизмов регуляторного действия окислителей и восстановителей в биологических системах. Окислители и восстановители рассматриваются как новый тип регуляторных молекул...
Article
Full-text available
Previously, we have reported that ascorbic acid regulates calcium signaling in human larynx carcinoma HEp-2 cells. To evaluate the precise mechanism of Ca(2+) release by ascorbic acid, the effects of specific inhibitors of the electron transport chain components on mitochondrial reactive oxygen species (ROS) production and Ca(2+) mobilization in HE...
Article
Full-text available
Рассмотрены современные представления о редокс-сигнализации и редокс-гомеостазе в опухолевых тканях. Детально проанализированы механизмы трансформации клеток, протекающие с участием активных форм кислорода. Особое внимание уделено новым технологиям в области противоопухолевой терапии, базирующимся на редокс-регуляции клеточных процессов.
Article
Full-text available
A model for the redox regulation of the functional state of the cell has been constructed on the basis of representation of electron transfer processes by equivalent electric circuits. The mechanism of action of redox-active molecules on biosystems has been discussed in terms of circuit theory. A method for determining the parameters of cellular re...
Article
Full-text available
Построена модель редокс-регуляции функционального состояния клетки на основе представления процессов переноса электронов с помощью эквивалентных электрических схем. С позиций теории цепей рассмотрен механизм действия редокс-активных молекул на биосистемы. Предложен способ определения параметров клеточных редокс-сенсоров. Установлено, что основными...
Article
Full-text available
Reactive oxygen species (ROS) are involved in a variety of biological phenomena, such as mutation, carcinogenesis, inflammation, aging, development, and signal transduction. Intracellular generation of ROS might lead to the activation of redox signaling or oxidative stress. Nonetheless, it is difficult to estimate whether ROS-induced intracellular...
Article
Full-text available
Redox regulation and carbohydrate recognition are potent molecular mechanisms which can contribute to platelet aggregation in response to various stimuli. The purpose of this study is to investigate the relationship between these mechanisms and to examine whether cell surface glycocalyx and cell stiffness of human platelets are sensitive to the red...
Article
Full-text available
The parameters of the acid-base state and redox state of erythrocytes have been studied with the use of the fluorescent probes 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein and 2',7'-dichlorodihydrofluorescein. It has been found that the value of redox state parameters in erythrocytes depends both on the extracellular and intracellular concentr...
Article
Full-text available
Effects of ascorbic acid on calcium homeostasis of human laryngeal carcinoma cells were studied. Intracellular concentration of free calcium and intracellular pH were measured by fluorescent analysis. Ascorbic acid in concentrations of 3-10 mM caused pH drop and sharply increased concentrations of free Ca ions in HEp-2 cells. Intracellular concentr...
Book
Full-text available
В пособии приведены современные представления о строении и функционировании биологических мембран. Детально рассмотрены физико-химические свойства липидов, структура и свойства мембранных белков и углеводного компонента мембран. Описаны механизмы рецепции и передачи информации с участием биологических мембран, механизмы транспорта веществ через био...
Article
Full-text available
В работе рассматриваются современные представления о механизмах регуляции редокс-гомеостаза биологических систем. Обсуждаются способы количественного описания редокс-процессов в биологических системах. Показано, что параметры редокс-гомеостаза необходимо рассматривать как регуляторные факторы трансдукции сигналов в клетках.
Article
Full-text available
The introduction of the parameters characterizing the redox state of the cell, such as the effective redox potential and the redox buffer capacity has been theoretically substantiated. A comparative study of the parameters of the redox state of erythrocytes from healthy donors and patients with diabetes and acute coronary syndrome has been performe...
Article
Full-text available
Recent achievements of biophysics and physiology resulted to remarkable progress in the understanding of role of redox processes in cell vital function. It was established that oxidizing and reducing agents participate in processes of differentiation, proliferation and apoptosis. Representations about an existence of definite balance between oxidat...
Book
Full-text available
В монографии обобщены и систематизированы современные представления об окислительно-восстановительных процессах, протекающих в клетках. Рассмотрены механизмы регуляторного и повреждающего действия биологических окислителей и восстановителей, физико-химические и биологические основы поддержания внутри-клеточного и тканевого окислительно-восстановите...
Article
Full-text available
The method for determination of intracellular oxidants content has been proposed. The method is based on the analysis of changes of 2,7-dichlorodihydrofluorescein oxidation. The theoretical model of hydrogen peroxide diffusion and its consumption in the cell and quantitative determination of hydrogen peroxide gradient through the cellular membrane...
Article
Full-text available
Redox state is a widely used term for the description of redox phenomena in biological systems. The regulating mechanisms responsible for maintaining the redox state are not yet fully known. But it was shown that changes in the redox state might lead to a cascade of intracellular events, beneficial or deleterious to the cell. There are several meth...
Article
Full-text available
It was proposed to use а concept of "redox buffer capacity" for the quantitative description of antioxidant biosystems property. Comparative analysis of redox buffer and pH-buffer properties were carried out. Dependence of redox buffer capacity value from reducing and oxidizing agents concentration was established. The theoretical estimation of red...
Article
Full-text available
It was proposed to use a concept of "effective redox potential" for the quantitative description of physical and chemical parameters of cellular oxidation-reduction state. Effective redox potential was calculated on the basis of thermodynamic ideas with use of the Nernst equation. The contribution of different oxidative and reductive reagents in th...
Article
Full-text available
В настоящей работе с использованием аппаратуры для внеклеточного мониторинга электрической активности нейронов проведено исследование электрической активности нервных ганглиев моллюска Lymnaea stagnalis при действии пероксида водорода Пероксид водорода в диапазоне концентраций от 0,5 ммоль/л до 20 ммоль/л индуцировал изменение частоты и амплитуды...
Article
Full-text available
Recent achievement of molecular biology and neurophysiology resulting in remarkable progress in the understanding of processes of neurodifferentiation, playing the relevant role both in an ontogenesis and working of adult brain. New representations have been formed about a role of cytoskeletal proteins in mechanisms of neurites growth. In the revie...
Article
Full-text available
Разработана методика измерения электрических характеристик нервных клеток и тканей с применением датчиков на основе МОП-транзисторов. Для защиты элементов датчика от воздействия компонентов культуральной среды разработан метод изоляции, основанный на использовании особенностей микроэлектродных компонентов в металлостеклянном корпусе. Для регистраци...
Conference Paper
Full-text available
This paper is devoted to description of a new type of information system based on interaction of biological neurons and electronic components - neuroelectronic (NE) system. Main features and components, like neural network sensor, of NE are briefly put into consideration followed with an example of electric interaction between neural network of a s...

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