Greta Forcaia

Greta Forcaia
Thermo Fisher Scientific | TFS · Divsion of Scientific Research

PhD in Neuroscience
Providing support and solutions to our customers for the improvement of their scientific research

About

14
Publications
2,651
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
223
Citations
Additional affiliations
April 2020 - present
Università degli Studi di Milano-Bicocca
Position
  • PostDoc Position
November 2016 - March 2020
Università degli Studi di Milano-Bicocca
Position
  • PhD Student
March 2016 - October 2016
San Raffaele Scientific Institute
Position
  • Fellow

Publications

Publications (14)
Article
We have uncovered a novel role for astrocytes-derived extracellular vesicles (EVs) in controlling intraneuronal Ca²⁺ concentration ([Ca²⁺]i) and identified transglutaminase-2 (TG2) as a surface-cargo of astrocytes-derived EVs. Incubation of hippocampal neurons with primed astrocyte-derived EVs have led to an increase in [Ca²⁺]i, unlike EVs from TG2...
Preprint
Full-text available
We have uncovered a novel role for astrocytes-derived extracellular vesicles (EVs) in controlling intraneuronal Ca2+ concentration ([Ca2+]i) and identified transglutaminase-2 (TG2) as a surface-cargo of astrocytes-derived EVs. Incubation of hippocampal neurons with primed astrocyte-derived EVs have led to an increase in [Ca2+]i, unlike EVs from TG2...
Article
Full-text available
In light of previous results, we assessed whether liposomes functionalized with ApoE-derived peptide (mApoE) and phosphatidic acid (PA) (mApoE-PA-LIP) impacted on intracellular calcium (Ca2+) dynamics in cultured human cerebral microvascular endothelial cells (hCMEC/D3), as an in vitro human blood-brain barrier (BBB) model, and in cultured astrocyt...
Article
Full-text available
Specific neurophysiological seizure patterns in patients with focal epilepsy depend on cerebral location and the underlying neuropathology. Location-specific patterns have been also reported in experimental models. Two focal seizure patterns, named p-type and l-type, typical of neocortical and mesial temporal regions were identified in both patient...
Article
Full-text available
Neurovascular coupling (NVC) is the mechanism whereby an increase in neuronal activity causes an increase in local cerebral blood flow (CBF) to ensure local supply of oxygen and nutrients to the activated areas. The excitatory neurotransmitter glutamate gates post-synaptic N-methyl-d-aspartate receptors to mediate extracellular Ca²⁺ entry and stimu...
Article
Full-text available
Oxygen diffusion across the air-blood barrier in the lung is commensurate with metabolic needs and ideally allows full equilibration between alveolar and blood partial oxygen pressures. We estimated the alveolo-capillary O2 equilibration in 18 healthy subjects at sea level at rest and after exposure to increased O2 demand, including work at sea lev...
Article
Full-text available
The neuromodulator histamine is able to vasorelax in human cerebral, meningeal and temporal arteries via endothelial histamine 1 receptors (H1 Rs) which result in the downstream production of nitric oxide (NO), the most powerful vasodilator transmitter in the brain. Although endothelial Ca 2+ signals drive histamine-induced NO release throughout th...
Article
Full-text available
The neurotransmitter glutamate increases cerebral blood flow by activating postsynaptic neurons and presynaptic glial cells within the neurovascular unit. Glutamate does so by causing an increase in intracellular Ca²⁺ concentration ([Ca²⁺]i) in the target cells, which activates the Ca²⁺/Calmodulin‐dependent nitric oxide (NO) synthase to release NO....
Article
Full-text available
Basal forebrain neurons control cerebral blood flow (CBF) by releasing acetylcholine (Ach), which binds to endothelial muscarinic receptors to induce nitric (NO) release and vasodilation in intraparenchymal arterioles. Nevertheless, the mechanism whereby Ach stimulates human brain microvascular endothelial cells to produce NO is still unknown. Here...
Article
Full-text available
Objective The key factors that promote the termination of focal seizures have not been fully clarified. The buildup of neuronal synchronization during seizures has been proposed as one of the possible activity‐dependent, self‐limiting mechanisms. We investigate if increased thalamo‐cortical coupling contributes to enhance synchronization during the...
Poster
The dilemma of the protection from noxious substances from the blood and the delivery of vital metabolites has always to be faced when dealing with the brain. Endothelial cells, forming the blood-brain barrier (BBB) with other cell types, regulate its trafficking. It is known that many common drugs cannot cross the BBB in appreciable concentration,...

Questions

Questions (2)
Question
I'd like to have some advices to perform calcium imaging in embryonic neuronal cultures.
Thanks to anybody who will help me
Question
I need some information to perform data analysis starting from the acquisition in time lapse

Network

Cited By