Gregory Salvignol

Gregory Salvignol
French National Centre for Scientific Research | CNRS · Institute of Analytical Science (ISA)

MS

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29
Publications
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2,352
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Publications

Publications (29)
Article
Full-text available
Global spread and limited genetic variation are hallmarks of M. tuberculosis, the agent of human tuberculosis. In contrast, Mycobacterium canettii and related tubercle bacilli that also cause human tuberculosis and exhibit unusual smooth colony morphology are restricted to East Africa. Here, we sequenced and analyzed the whole genomes of five repre...
Article
Full-text available
MicroScope is an integrated platform dedicated to both the methodical updating of microbial genome annotation and to comparative analysis. The resource provides data from completed and ongoing genome projects (automatic and expert annotations), together with data sources from post-genomic experiments (i.e. transcriptomics, mutant collections) allow...
Data
Genomics Islands. Genomic Island predicted in CMR15 (sheet 1 and 2), CFBP2957 (sheet 3 and 4) and PSI07 (sheet 5 and 6) genomes.
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Metabolic pathways. Sheet A: class of each pathway class according to figure 6. Sheet B: Pathway completion.
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new CDS GMI1000. New CDS detected in GMI1000 chromosome (sheet 1) and megaplasmide (sheet2).
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genomes overview. Main features of CMR15, CFBP2957, PSI07, GMI1000, Molk2 and IPO1609 genomes.
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strains (microarray). Strains used in CGH microarray experiments.
Article
Full-text available
The Ralstonia solanacearum species complex includes thousands of strains pathogenic to an unusually wide range of plant species. These globally dispersed and heterogeneous strains cause bacterial wilt diseases, which have major socio-economic impacts. Pathogenicity is an ancestral trait in R. solanacearum and strains with high genetic variation can...
Data
Circular representations of Thiomonas chromosome and pTHI plasmid. Gene organization found in (A) the Thiomonas sp. 3As chromosome. The localisation of the 19 GEIs (A-S) is schematized with grey triangles; (B) the plasmid pTHI. Circles display (from the outside): (1) GC percent deviation (GC window - mean GC) in a 1000-bp window; (2) Predicted CDSs...
Data
Expression of ars Operons and Synthesis of the Corresponding Proteins in Response to Arsenic (A) Effect of arsenic on ars operon expression. The level of expression was evaluated by determining the mRNA content of the transporter-encoding gene, i.e., the arsB pump in operons I and II and acr3 transporter in operon 3, by slot-blotting hybridisation...
Data
Genomic Islands (GEIs) or islets found in the Thiomonas sp. 3As genome. (0.04 MB XLS)
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Phylogenetic analysis of the 75 genes contained in the ThGEI-L island. (0.04 MB XLS)
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Phylogenetic analysis of the 121 genes contained in the ThGEI-0 island. (0.05 MB XLS)
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PCR targets and GenBank Accession IDs of strains used in this study. (0.03 MB DOC)
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Arsenic Stress and DNA Repair (A) Effect of arsenic on gene expression. The expression of several genes known to play a role in DNA recombination and repair and/or stress adaptation (Table 2) was evaluated by comparing their mRNA content by slot-blotting hybridisation experiments after exposure to 300 ppm As[III] for 15 min. The ribosomal rrn mRNA...
Data
Arsenite Oxidase Activity in H. arsenicoxydans Wild-Type and Mutant Strains Strains tested for oxidation of arsenite to arsenate were grown for 24 h at 25 °C on CDM agar plates supplemented with arsenite (100 mg/l). Agar plates were then flooded with a solution of 0.1 M AgNO3. A brownish precipitate revealed the presence of arsenate in the medium s...
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List of ISs present in Thiomonas sp. 3As genome and plasmid. (0.05 MB XLS)
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Description of the H. arsenicoxydans GC-Rich Island Locus tag, gene name, and description for H. arsenicoxydans are colored according to Figure 2 (red for arsIII cluster, blue for the part of the clc element, and green for the more specific metabolic capabilities found in the third part of this island). For each of the proteobacteria compared, the...
Article
Full-text available
Author Summary Recent advances in the field of arsenic microbial metabolism have revealed that bacteria colonize a large panel of highly contaminated environments. Belonging to the order of Burkholderiales, Thiomonas strains are ubiquitous in arsenic-contaminated environments. The genome of one of them, i.e. Thiomonas sp. 3As, was deciphered and co...
Article
Full-text available
The initial outcome of genome sequencing is the creation of long text strings written in a four letter alphabet. The role of in silico sequence analysis is to assist biologists in the act of associating biological knowledge with these sequences, allowing investigators to make inferences and predictions that can be tested experimentally. A wide vari...
Data
Methylotrophic bacteria with published genome sequences included in comparative analyses (0.04 MB DOC)
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Methylotrophy genes in M. extorquens AM1 and DM4 (0.07 MB DOC)
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Methylotrophy enzymes and pathways deduced from complete genomic sequences of methylotrophs (0.04 MB DOC)
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Characteristics of IS elements in Methylobacterium extorquens (0.05 MB DOC)
Article
Full-text available
Methylotrophy describes the ability of organisms to grow on reduced organic compounds without carbon-carbon bonds. The genomes of two pink-pigmented facultative methylotrophic bacteria of the Alpha-proteobacterial genus Methylobacterium, the reference species Methylobacterium extorquens strain AM1 and the dichloromethane-degrading strain DM4, were...
Article
Full-text available
The remarkable repetitive elements called CRISPRs (clustered regularly interspaced short palindromic repeats) consist of repeats interspaced with non-repetitive elements or 'spacers'. CRISPRs are present in both archaea and bacteria, in association with genes involved in DNA recombination and repair. In the Yersinia pestis genome, three such elemen...

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