Gregory Lambert

Gregory Lambert
TargEDys · General Management

Pharm.D., Ph.D.

About

79
Publications
27,238
Reads
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2,757
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Introduction
Experienced pharmaceutical industry executive with a successful track record in product development, business development, fund raising and general management in international and multi-cultural environments. - 15 years of experience in late stage biotech and in pharmaceutical industry - Development of pharmaceutical products and medical devices from concept to registration - Business development and licensing - Strategic and Operational skills
Additional affiliations
December 2014 - present
TargEDys
Position
  • CEO / CSO
November 2008 - August 2014
Septodont
Position
  • Research Officer
October 2000 - October 2008
Santen
Position
  • CEO
Education
September 1996 - October 2000
Université Paris-Sud 11
Field of study
  • Pharmaceutical technology

Publications

Publications (79)
Article
Full-text available
Abstract Purpose: Benzalkonium chloride (BAK), a common preservative in eye drops, can induce ocular surface toxicity that may decrease glaucoma therapy compliance. The ocular hypotensive effect, pharmacokinetic (PK) profiles, and local tolerance of a preservative-free latanoprost 0.005% cationic emulsion (Catioprost(®)), and a BAK-preserved latano...
Article
Full-text available
Objectives A multicentric randomized, 3-year prospective study was conducted to determine for how long Biodentine, a new biocompatible dentine substitute, can remain as a posterior restoration. Materials and methods First, Biodentine was compared to the composite Z100®, to evaluate whether and for how long it could be used as a posterior restoratio...
Article
Full-text available
Antisense oligonucleotides with base sequences complementary to a specific RNA can, after binding to intracellular mRNA, selectively modulate the expression of a gene. However, these molecules are poorly stable in biological fluids and are characterized by a low intracellular penetration. In view of using oligonucleotides as active molecules, the d...
Article
Full-text available
The aim of the present study was to evaluate the inhibitory effect on protein kinase C alpha (PKC alpha) neosynthesis of antisense oligonucleotides delivered by two types of carriers. First, PKC alpha antisense oligonucleotides were associated with polyisobutylcyanoacrylate (PIBCA) nanoparticles pre-coated with cetyltrimethyl ammonium bromide (CTAB...
Article
Full-text available
Antisense oligonucleotides are molecules that are able to inhibit gene expression being therefore potentially active for the treatment of viral infections or cancer. However, because of their poor stability in biological medium and their weak intracellular penetration, colloidal drugs carriers such as nanoparticles were developed for the delivery o...
Article
Full-text available
CLPB (Caseinolytic peptidase B) protein is a conformational mimetic of �-MSH, an anorectic hormone. Previous in vivo studies have already shown the potential effect of CLPB protein on food intake and on the production of peptide YY (PYY) by injection of E. coli wild type (WT) or E. coli DClpB. However, until now, no study has shown its direct effec...
Article
Full-text available
Background: Increasing evidence supports the role of the gut microbiota in the control of body weight and feeding behavior. Moreover, recent studies have reported that the probiotic strain Hafnia alvei HA4597® (HA), which produces the satietogenic peptide ClpB mimicking the effect of alpha-MSH, reduced weight gain and adiposity in rodent models of...
Patent
The present invention relates to pharmaceutical and food compositions for inducing satiation and prolonging satiety in subjects in need thereof. In particular, the present invention relates to a method of inducing satiation in a subject in need thereof comprising administering to the subject an effective amount of a ClpB protein or an effective amo...
Patent
Methods of treating inflammation, in particular obesity, inducing satiation, prolonging satiety, and stimulating weight loss in a subject in need thereof, including the administration of a Brassicaceae protein extract. The Brassicaceae protein extract may be administered in the form of a dietary supplement or a food composition, either of which may...
Article
Introduction et but de l’étude Le microbiote intestinal vit en relation symbiotique forte avec l’hôte et participe activement au contrôle du poids corporel par le biais de la production de protéines, peptides et métabolites [1]. La souche probiotique H. alvei HA4597® (HA4597) a été adaptée pour produire en grande quantité la protéine « caséinolytiq...
Patent
The present invention relates to method of preventing, treating or attenuating obesity-related manifestations in a human or non-human mammal subject in need thereof, said method comprising orally administering to the subject a composition comprising an effective amount Hafnia alvei probiotics; wherein said obesity-related parameters are selected fr...
Article
Full-text available
Based on the recent identification of E.coli heat shock protein ClpB as a mimetic of the anorexigenic α-melanocyte stimulating hormone (α-MSH), the objective of this study was to preclinically validate Hafnia alvei, a ClpB-producing commensal bacterium as a potential probiotic for appetite and body weight management in overweight and obesity. The i...
Presentation
Introduction et but de l’étude Le microbiote contribue à l’homéostasie énergétique et à la régulation de du comportement alimentaire et ses modifications pourraient être impliquées dans la physiopathologie de l’anorexie mentale. La protéine ClpB (Caseinolytic peptidase B), produite principalement par les Enterobacteriaceae, a été identifiée comme m...
Article
Full-text available
Eating disorders (EDs) are increasingly frequent. Their pathophysiology involves disturbance of peptide signaling and the microbiota–gut–brain axis. This study analyzed peptides and corresponding immunoglobulin (Ig) concentrations in groups of ED. In 120 patients with restrictive (R), bulimic (B), and compulsive (C) ED, the plasma concentrations of...
Article
Full-text available
Use of new generation probiotics may become an integral part of the prevention and treatment strategies of obesity. The aim of the present study was to test the efficacy of a potential probiotic strain of lactic bacteria Hafnia alvei (H. alvei) HA4597™, in a mouse model of obesity characterized by both hyperphagia and diet-induced adiposity. For th...
Article
Full-text available
Microbiota contributes to the regulation of eating behavior and might be implicated in the pathophysiology of anorexia nervosa. ClpB (Caseinolytic peptidase B) protein produced mainly by the Enterobacteriaceae family has been identified as a conformational mimetic of α-MSH, which could result in similar anorexigenic effects. The aim of this study w...
Article
Full-text available
Gut microbiota can influence the feeding behavior of the host, but the underlying mechanisms are unknown. Recently, caseinolytic protease B (ClpB), a disaggregation chaperon protein of Escherichia coli, was identified as a conformational mimetic of α-melanocyte-stimulating hormone (α-MSH), an anorexigenic neuropeptide. Importantly, ClpB was necessa...
Article
Full-text available
Background: Eating disorders (EDs) lead to multiple psychiatric and somatic complications and thus constitute a major public health concern. Objectives: The aim of this study was to give an exhaustive view of the studies reporting the prevalence of the different EDs or total EDs and to study their evolution. Methods: A literature search follow...
Article
Discipline Expérimental/mécanismes cellulaires et moléculaires. Introduction et but de l’étude L’obésité et les troubles du comportement alimentaire (TCA) sont des problèmes majeurs de santé publique, dont la physiopathologie est multifactorielle. La régulation du comportement alimentaire implique des boucles neurohormonales liées à l’axe microbio...
Article
Discipline Expérimental/mécanismes cellulaires et moléculaires. Introduction et but de l’étude La prévalence du surpoids et l’obésité est en sévère augmentation à travers le Monde et s’expose à de nombreuses comorbidités. Dans l’apparition et l’évolution de ces pathologies multifactorielles, le microbiote intestinal pourrait avoir un rôle clé. En...
Article
Discipline Épidémiologie. Introduction et but de l’étude Les troubles du comportement alimentaire (TCA) sont des perturbations sévères, qui peuvent conduire à de multiples complications tant psychologiques que somatiques et sont susceptibles d’impacter la qualité de vie et la mortalité du patient. Les TCA les mieux caractérisés sont l’anorexie, la...
Article
Discipline Expérimental/mécanismes cellulaires et moléculaires. Introduction et but de l’étude De nombreuses altérations du microbiote intestinal (ou dysbioses) ont été recensées chez des patients atteints d’obésité ou d’anorexie. En effet, il a été démontré une augmentation de la population appartenant à l’ordre des Enterobacteriaceae et notammen...
Article
Full-text available
Discipline Clinique. Introduction et but de l’étude Les troubles du comportement alimentaires (TCA) sont des pathologies fréquentes, dont la physiopathologie implique l’axe microbiote-intestin-cerveau. Plusieurs neuropeptides et hormones sont impliqués dans la régulation du comportement alimentaire et du métabolisme énergétique. Des études ont mis...
Article
Full-text available
Although local anesthetics (LAs) are generally accepted as being safe, incidental neuronal damage has been reported for all LAs in humans. Therefore, in this study, we compared the dose corresponding to 50% cell lethality (LD50) of articaine, lidocaine, mepivacaine, bupivacaine, prilocaine, and ropivacaine in human neuroblastoma cells. Undifferenti...
Conference Paper
Full-text available
Objective: During long procedures and surgery, a pathogen-free environment is a main concern. A disposable safety injection system (Ultra Safety Plus) is demonstrated to reduce the risk of needle stick injury for the practitioner. Because the device is covering the anesthetic cartridge, it was hypothetized that it could also act as a shield to redu...
Patent
Full-text available
The present invention relates to a new therapeutic approach for the treatment of eye diseases or conditions that allows for the delivery of corticosteroids to the eye's surface but does not cause any of the usual side effects associated with topical administration of corticosteroids. More specifically, the present invention provides methods of trea...
Patent
Full-text available
Composition containing quaternary ammonium compounds in which the nitrogen atom is substituted by at least one alkyl group having at least 12 carbon atoms, the composition including at least 20% in weight by weight of the total composition, of ammonium halides in which the nitrogen atom is substituted by at least one alkyl group having at least 14...
Patent
Full-text available
Oil-in-water emulsion including a non-steroidal anti-inflammatory drug and a quaternary ammonium halide useful for the prevention and treatment of inflammation in the eye, and process for manufacturing thereof.
Patent
Full-text available
An ophthalmic oil-in-water type emulsion, which includes colloid particles having an oily core surrounded by an interfacial film, the emulsion including at least one cationic agent, at least one no ionic surfactant, the emulsion having a positive zeta potential and meeting zeta potential stability Test A requirements. Process for making the emulsio...
Article
Objectives: To compare the effect on insertion and withdrawal forces using needles with a standard atraumatic bevel to those with a novel asymmetrical bevel in vitro. Methods: Maximum needle penetration forces were measured using a 10N load cell mounted on an Instron universal testing machine. Forces were determined during insertion through a 0....
Patent
Full-text available
A well tolerated oil-in-water emulsion useful as a delivery vehicle of hydrophobic ingredients such as pharmaceutical drugs, wherein the emulsion particles have a net positive charge and comprises 0.001 to 0.1% of a cationic agent, 0 to 1% of a non ionic surfactant and 0 to 0.5% of an anionic surfactant.
Patent
Full-text available
Ophthalmic oil-in-water emulsions, which comprises colloid particles having an oily core surrounded by an interfacial film, the emulsion comprising an 10 immunosuppressive agent, an oil, preferably at least 50% of which being MCT, and tyloxapol. Use of such an emulsion for the manufacture of medicament for treatment of eye conditions, particularly...
Article
Purpose: The aim of this study was to compare the ocular and systemic distribution of cyclosporine A (CsA) in rabbits after the instillation of preservative-free CsA cationic and anionic emulsions. Methods: For the single-dose pharmacokinetic (PK) study, rabbits were instilled with 50 μL of the test material. For the multiple-dose PK study, rabb...
Patent
Full-text available
Cationic ophthalmic oil-in-water type emulsions, include colloid particles having an oily core surrounded by an interfacial film, the emulsion including at least one cationic agent and at least one non ionic surfactant, the oily core including a prostaglandin selected from the group comprising in particular latanoprost, unoprostone isopropyl, travo...
Patent
Full-text available
Use of a composition comprising at least one prodrug of a steroid, preferably of a corticosteroid, for the preparation of an ophthalmic composition intended for the treatment of an ocular condition or disease of a human being or an animal.
Conference Paper
Full-text available
Objective: The neurotoxicity of local anesthetics (LA) has been assessed in neuronal cells (Perez-Castro et al., 2009; Werdehausen et al., 2009). Our study aimed to determine the dose corresponding to 50% cell lethality (LD50) of articaine, lidocaine, mepivacaine, bupivacaine, prilocaine and ropivacaine in human neuroblastoma cells. Method: Undif...
Patent
Full-text available
Composition containing quaternary ammonium compounds in which the nitrogen atom is substituted by at least one alkyl group having at least 12 carbon atoms, the composition including at least 20% in weight by weight of the total composition, of ammonium halides in which the nitrogen atom is substituted by at least one alkyl group having at least 14...
Conference Paper
Full-text available
Purpose:The studies were designed to compare the ocular hypotensive effects, PK profiles, and ocular safety of the BAK-free latanoprost emulsion Catioprost® and the BAK-preserved latanoprost solution Xalatan® in cynomologus monkeys with glaucoma and rabbits, respectively. Methods:For the efficacy evaluation, glaucomatous eyes (high IOP was induced...
Conference Paper
Full-text available
Purpose 1. Rabinovich-Guilatt L et al. Use of a steroid prodrug for the treatment of disease of the posterior segment of the eye. US Patent US20070281914. 2006. 2. Rabinovich-Guilatt L et al. Method for treating eye disease or conditions affecting the posterior segment of the eye. US Patent US20070280902. 2006. 3. Safety and Tolerability of NOVA630...
Conference Paper
Full-text available
Ophthalmic oil-in-water type emulsion with stable positive zeta potential. US 20070248645. Novagali Pharma SA, 2007. 2. Lallemand F, Felt-Baeyens O, Besseghir K, Behar-Cohen F, Gurny R. Cyclosporine A delivery to the eye: A pharmaceutical challenge. Eur J Pharm Biopharm. 2003; 56(3):307-18. 3. Abdulrazik M, Tamilvanan S, Khoury K, Benita S. Ocular...
Conference Paper
Full-text available
Purpose 1. Bhattacherjee P, Paterson CA, Spellman JM, Graff G, Yanni JM. Pharmacological validation of a feline model of steroid-induced ocular hypertension. Arch Ophthalmol. 1999; 117(3):361-4. 2. Rabinovich-Guilatt L et al. Use of a steroid prodrug for the treatment of disease of the posterior segment of the eye. US Patent US20070281914. 2006. 3....
Conference Paper
Full-text available
Objectives: To compare the performance of a standard dental needle to one with a scalpel-design bevel in vitro. Methods: Maximum needle penetration forces in Newtons (N) were measured using a 10N load cell mounted on an Instron universal testing machine in a series of tests. In one series these forces were determined during insertion through a 0.4m...
Conference Paper
Full-text available
Objectives: The aim of the study was to collect follow-up information on the symptoms and duration of paresthesia pharmacovigilance cases. Methods: All cases of paresthesia, numbness or hypoesthesia, in procedures involving articaine and reported by USA dentists from March 2005 to December 2009 were extracted from the database. Surgical and drug...
Patent
Full-text available
Composition comprising at least one nucleosidic moiety and cetalkonium chloride and pharmaceutical use thereof for prevention, treatment or relief of eye, lung, and/or respiratory tract conditions.
Article
Full-text available
Antisense oligonucleotides (ODNs) specific for VEGFR-2-(17 MER) and inhibiting HUVEC proliferation in-vitro were screened. One efficient sequence was selected and incorporated in different types of nanoemulsions the potential toxicity of which was evaluated on HUVEC and ARPE19 cells. Our results showed that below 10 microl/ml, a 2.5% mid-chain trig...
Article
Full-text available
The anticancer drug paclitaxel is formulated for i.v. administration in a mixture of Cremophor EL and ethanol. Its oral bioavailability is very low due to the action of P-glycoprotein in the gut wall and CYP450 in gut wall and liver. However, proof-of-concept studies using the i.v. formulation diluted in drinking water have demonstrated the feasibi...
Article
Full-text available
Using an established rabbit toxicological model, this in vivo study compared the ocular cytotoxicity of four topical intraocular pressure (IOP)-lowering agents: the commercial benzalkonium chloride (BAC)-containing solutions of 0.005% latanoprost, 0.004% travoprost, 0.03% bimatoprost (containing 0.02%, 0.015%, and 0.005% BAC, respectively), and 0.0...
Article
Full-text available
Novel formulations of cationic nanoemulsions based on three different lipids were developed to strengthen the attraction of the polyanionic oligonucleotide (ODN) macromolecules to the cationic moieties on the oil nanodroplets. These formulations were developed to prolong the release of the ODN from the nanoemulsion under appropriate physiological d...
Article
Full-text available
To evaluate and compare the toxicological profiles of two quaternary ammonium compounds (QAC), benzalkonium chloride (BAK), and cetalkonium chloride (CKC), in standard solution or cationic emulsion formulations in rabbit eyes using newly developed in vivo and ex vivo experimental approaches. Seventy eyes of 35 adult male New Zealand albino rabbits...
Article
Full-text available
To explore the parmacokinetics, safety and tolerability of paclitaxel after oral administration of SMEOF#3, a novel Self-Microemulsifying Oily Formulation, in combination with cyclosporin A (CsA) in patients with advanced cancer. Seven patients were enrolled and randomly assigned to receive oral paclitaxel (SMEOF#3) 160 mg+CsA 700 mg on day 1, foll...
Article
Full-text available
In an attempt to design a targeted drug delivery system to tumors' over-expressing H-ferritin specifically recognized by a monoclonal antibody, AMB8LK, a cationic emulsion - AMB8LK conjugate was prepared. A novel cross-linker molecule bearing maleimide group was synthesized and added to cationic emulsion formulation for AMB8LK Fab' fragment covalen...
Article
The aim of this work was to develop a simple high-performance liquid chromatography (HPLC) technique with evaporative light scattering detection (ELSD) for the separation and quantification of the major phospholipid (PL) and lysophospholipid (LPL) classes contained in a pharmaceutical phospholipid-based emulsion. In the established method, phosphat...
Article
New and fascinating advances in biotechnology have open new perspectives since the use of monoclonal antibodies for therapeutic application, particularly cancer, has been approved. Over the last two decades, numerous research teams have demonstrated that the progression of cancer is often associated with an over-expression of one or several protein...
Article
Full-text available
New and fascinating advances in biotechnology have open new perspectives since the use of monoclonal antibodies for therapeutic application, particularly cancer, has been approved. Over the last two decades, numerous research teams have demonstrated that the progression of cancer is often associated with an over-expression of one or several protein...
Patent
A combination product comprising a positive oil in water emulsion wherein said emulsion comprises a compound presenting free NH2 groups, at its natural state, at the oil-water interface, and an antibody, wherein said compound is linked to said antibody by a heterobifunctional linker, linking said NH2 groups to SH groups on the antibody hinge region...
Article
Full-text available
Pulmonary immunization against inhaled pathogens such as Mycobacterium tuberculosis would induce local and systemic immune responses and protect from entry and dissemination of the pathogen. The aim of this study was to evaluate cationic submicron emulsion as a potential carrier for DNA vaccines to the lung. DNA loaded emulsions were 128-152 nm in...
Article
Full-text available
The present study is aimed to characterize the electrostatic parameters of oil in water emulsion droplets composed of MCT (medium chain triglycerides), PL (phospholipids) and Poloxamer and containing increasing concentrations of the cationic lipid oleylamine (OA), in Hepes 20 mM pH 7.4. The initial zeta-potential data suggesting saturation of the d...
Article
Full-text available
The objective of this study was to evaluate the pharmacokinetics of paclitaxel in a novel self-microemulsifying drug delivery system (SMEDDS) for improved oral administration with or without P-glycoprotein (P-gp) inhibitors. Paclitaxel SMEDDS formulation was optimized, in terms of droplet size and lack of drug precipitation following aqueous diluti...