Graziella Midelet-Bourdin

Graziella Midelet-Bourdin
Agence Nationale de Sécurité Sanitaire de l'Alimentation, de l'Environnement et du Travail | ANSES · Boulogne-sur-Mer Laboratory for Fishery Products

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43
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Publications

Publications (43)
Article
Full-text available
From a global view of antimicrobial resistance over different sectors, seafood and the marine environment are often considered as potential reservoirs of antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs); however, there are few studies and sparse results on this sector. This study aims to provide new data and insights regardi...
Article
Full-text available
The marine environment is a potential natural reservoir of antimicrobial resistance genes (ARGs), subject to anthropogenic effluents (wastewater, industrial, and domestic), and known as a final receiving system. The aim of this study was to investigate the abundance and geographical distribution of the three blaTEM, sul1, and intI1 genes, proposed...
Article
Full-text available
Trematode larvae (metacercariae) causing black spot disease occur frequently in gills, fins, skin and the superficial muscle layers of marine fish. Species within the genus Cryptocotyle Lühe, 1899 are frequently associated with this disease. Descriptions of the metacercarial stage are relatively limited and none has hitherto been reported from fish...
Article
Full-text available
Some trematode metacercariae, including marine digeneans belonging to the genus Cryptocotyle, induce black spots in target tissues due to the attraction of fish host melanophores. To promote precise quantification of infection, the counting of black spots has to be confirmed by reliable quantification of metacercariae after tissue digestion. This p...
Article
The superfamily Opisthorchioidea encompasses the families Cryptogonimidae, Opisthorchiidae and Heterophyidae. These parasites depend on the aquatic environment and include marine and freshwater species. Some species, such as Clonorchis sinensis and Opisthorchis viverrini, have a high impact on public health with millions of infected people worldwid...
Article
Full-text available
We evaluated the performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) associated with the Bruker BioTyper TM V7.0.0 database for the identification of 713 bacterial strains isolated from seafood products and sea water samples (ANSES B3PA collection) under culture conditions that may have been si...
Article
Vibrio sp., ubiquitous in the aquatic ecosystem, are bacteria of interest because of their involvement in human health, causing gastroenteritis after ingestion of seafood, as well as their role in vibriosis leading to severe losses in aquaculture production. Their ability to enter a viable but non-culturable (VBNC) state under stressful environment...
Article
The objectives of this study were 1) to evaluate the impact of two industrial disinfectants on the viability of Listeria monocytogenes populations in biofilm and 2) to investigate the viability state of L. monocytogenes cells present on contact surfaces in the smoked salmon processing environment. In the first step, we cultured mono species and mix...
Article
Full-text available
The ready-to-eat products can be contaminated during processing by pathogen or spoilage bacteria, which persist in the industrial environment. Some bacterial species are able to form biofilms which protect them from environmental conditions. To check the bacterial contamination of the surfaces in the food industries, the professionals must regularl...
Article
Aims The aim of this study was to evaluate the performance of sampling methods [contact plates, sponges, and swabs] in the recovery of biofilm Listeria monocytogenes populations considering the seafood environment conditions (nature of conditioning, of materials and bacterial species). Methods and results Different materials (stainless steel, poly...
Article
Full-text available
Listeria monocytogenesClonal Complexes (CCs) have been epidemiologically associated with foods, especially ready-to-eat (RTE) products for which the most likely source of contamination depends on the occurrence of persisting clones in food-processing environments (FPEs). As the ability of L. monocytogenes to adapt to environmental stressors met in...
Article
A single strain of Vibrio parahaemolyticus and a single stain of Vibrio cholerae were isolated from shrimps sourced from seafood processing plants. The isolated strains were evaluated for their ability to adhere to stainless steel surfaces, under conditions encountered in seafood industries. In this way, the impact of different environmental factor...
Chapter
Foodborne pathogens are responsible of foodborne diseases and food poisoning and thus pose a great threat to food safety. These microorganisms can adhere to surface and form a biofilm composed of an extracellular matrix. This extracellular matrix protects bacterial cells from industrial environmental stress factors such as cleaning and disinfection...
Presentation
The viable but not culturable (VBNC) state is a survival strategy implemented by bacteria in response to adverse environmental conditions. VBNC bacteria remain metabolically active but can no longer be detected on conventional culture media. These characteristics of VBNC cells pose a risk for food safety as pathogens may not be detected in the proc...
Article
Full-text available
The wall teichoic acid (WTA) is the major carbohydrate found within the extracellular matrix of the Listeria monocytogenes biofilm. We first addressed the frequency of spontaneous mutations in two genes (lmo2549 and lmo2550) responsible for the GlcNAcylation in 93 serotype 1/2a strains that were mainly isolated from seafood industries. We studied t...
Data
Accession numbers of any nucleotide sequence data. (PDF)
Data
Dendrogram of obtained using ApaI and AscI enzymes demonstrating the genetic diversity of L. monocytogenes isolates. (PDF)
Data
Growth of the wild-type EGD-e strain, DSS 1130 BFA2, EGD-eΔlmo2549 and EGD-eΔlmo2550 mutants and the complemented mutants EGD-e Δlmo2549:: pLIV2(lmo2549) and EGD-e Δlmo2550:: pLIV2(lmo2550) at 30°C in MCDB202 medium during 48 h. (PDF)
Article
This study was designed to assess the efficiency of eight extraction methods regarding their ability to release superficial (exogenous) and intracellular (endogenous) DNA from B. cereus spores for subsequent analysis by quantitative PCR (qPCR). B. cereus spore suspensions were subjected to both commercial DNA extraction kits and mechanical DNA extr...
Article
Seafood products are widely consumed all around the world and play a significant role on the economic market. Bacteria of the Vibrio genus can contaminate seafood and thus pose a risk to human health. Three main Vibrio species, V. cholerae, V. parahaemolyticus and V. vulnificus, are potentially pathogenic to humans. These species are responsible fo...
Poster
Full-text available
Biofilms form ecological niches enhancing bacterial tolerance to cleaning and disinfection treatments and thus contributing to the persistence of these microorganisms in food-processing environments. Those biofilms can be involved in food contamination and consequently need to be better characterized in order to evaluate their impact on food safety...
Poster
Full-text available
Various types of surface are used in the food industry and are subject to contamination by microorganisms which can be organized into biofilm. Biofilm is a microorganism complex community that adhere on surface and embedded in an extracellular matrix. The objective of this study was therefore to evaluate the effectiveness of four recovery methods d...
Poster
Listeriosis is caused by the food-borne pathogen Listeria monocytogenes, which can be found in seafood products and processing plants. Furthermore, it can form biofilms with dead, viable culturable and viable but non culturable cells (VBNC) bacterial populations, which makes it resistant against cleaning and disinfection operations. The microbiolog...
Article
The aim of this study was to characterize the Listeria monocytogenes biofilm and particularly the nature of the carbohydrates in the biofilm extracellular matrix and culture supernatant versus to cell wall carbohydrates. L. monocytogenes serotype 1/2a and 4b strains were able to form complex biofilms embedded in an extracellular matrix. The soluble...
Conference Paper
Full-text available
Shewanella putrefaciens has been described in numerous studies as the specific spoilage organism of marine fish conserved on melting ice. S. putrefaciens is more likely a bacterial group divided into four sub-groups than a single species; subgroup II being only composed by Shewanella baltica. In most cases, isolation of Shewanella strains involves...
Article
Full-text available
Unlabelled: Four strains were isolated from a spoiled whiting (Merlangius merlangus). All of them were able to grow aerobically from 4 to 30°C and also able to develop anaerobically in the presence of trimethylamine N-Oxide (TMAO) at 25°C. Biochemical characterization did not allow identification of the strains species but showed that one of the f...
Article
Bacillus strains are often isolated from biofilms in the food industries. Previous works have demonstrated that sporulation could occur in biofilms, suggesting that biofilms would be a significant source of food contamination with spores. In this study, we investigated the properties of mono-species and mixed Bacillus biofilms and the ability of Ba...
Article
The purpose of this study was to quantify the extracellular matrix of Listeria monocytogenes biofilm. A preliminary study was carried out to establish a relationship between phylogenetic lineage of 27 strains and their ability to form biofilm in various conditions. Biofilm formation on microtitre plates of 27 strains of L. monocytogenes belonging t...
Article
The assessment of the evolution of micro-organisms naturally contaminating food must take into account the variability of biological factors, food characteristics and storage conditions. A research project involving eight French laboratories was conducted to quantify the variability of growth parameters of Listeria monocytogenes obtained by challen...
Article
The aim of this work was to evaluate the increase of Listeria monocytogenes on new salmon preparations (salt–sugar–pepper–dill salmon) in comparison with that obtained on cold-smoked salmon. Salmon preparations were inoculated with L. monocytogenes and were analyzed during storage at 4°C then 8°C. At 8°C, the bacteria growth was of 4.53 logCFUg−1 i...
Article
The aim of this study was to investigate the impact of superchilling (−2 °C) on the evolution of Listeria monocytogenes and organoleptic characteristics of cold-smoked salmon samples. An Hadamard matrix experimental design was carried out on artificially inoculated samples stored at +4 °C for 10 d and at +8 °C for 18 d to know the influence of four...
Article
Detection and enumeration of Listeria monocytogenes and total spoilage bacteria in 40 batches of cold-smoked salmon (one batch = 42 products from the same day of manufacture) straight from the factory were carried out. If L. monocytogenes was detected in at least one of the nine samples analyzed on receipt at the laboratory, 9 products of the same...
Article
Detection and enumeration of Listeria monocytogenes and total spoilage bacteria in 40 batches of cold-smoked salmon (one batch = 42 products from the same day of manufacture) straight from the factory were carried out. If L. monocytogens was (]elected in at least one of the nine samples analyzed oil receipt at the laboratory, 9 products of the same...
Article
Full-text available
One hundred one strains of Listeria monocytogenes isolated from seafood and cheese industry samples and from patients with listeriosis were assessed using a microtiter plate method for adhesion to polystyrene and stainless steel surfaces. The adhesion rate for these strains ranged from 3.10 to 35.29% with an inoculum of 8 x 10(8) cells per well. A...
Article
Retail seafood products were analyzed on their use-by date using the international reference methods NF EN ISO 11290-1 and 11290-2 (collectively method R) or an in-house method (method B) for the isolation of Listeria monocytogenes. The sensitivity of the methods was about 78%. Method R detected more positive samples of smoked salmon and herb-flavo...
Article
To assess the effect of different foods, which have been implicated or not in cases of listeriosis, on the in vitro virulence-associated phenotype level of different Listeria monocytogenes strains. The virulence-associated phenotype level of L. monocytogenes was studied with the in vitro cell test based on a plaque-forming assay with a human adenoc...
Article
The aim of this study was to establish which of seven factors influence the adhesion strength and hence bacterial transfer between biofilms containing Listeria monocytogenes (pure and two-species biofilms) and tryptone soya agar (TSA) as a solid organic surface. The two-species biofilms were made of L. monocytogenes and one of the following species...
Article
To determine how single cells and microcolonies transfer to food from open surfaces in the meat industry. Biofilms of four bacterial strains isolated from food processing surfaces were established on stainless steel substrates conditioned with meat exudate in the presence or absence of CaCl(2). Image analysis of the biofilms showed that the additio...
Article
The quantity of microorganisms that may be transferred to a food that comes into contact with a contaminated surface depends on the density of microorganisms on the surface and on the attachment strengths of the microorganisms on the materials. We made repeated contacts between pieces of meat and various surfaces (stainless steel and conveyor belt...

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