Gopi Ragupathi

• Ph.D
• Professor (Associate) at Annamalai University

Blanching is a primary step in processing of Green Leafy Vegetables. Despite of its preserving advantage, it leads to partial destruction of some nutrients like vitamin C which is heat liable and sensitive to light, oxygen and oxidizing agents. The study was carried out to identify a suitable blanching temperature, time and chemical media for the g...

Gloriosa superba Linn. is one of the important medicinal plant now in endangered list. This plant is widely used for several ethano-medicinal purposes by tribal peoples and traditional practitioners. Seeds and tubers contain valuable alkaloids viz., colchine and colchicoside as the major constituents, which are used an antidote for snake bites, gou...

Probiotics are live microbes which when administered in adequate amounts as functional food ingredients confer a health benefit on the host. Their versatility is in terms of their usage which ranges from the humans to the ruminants, pigs and poultry, and also in aquaculture practices. In this review, the microorganisms frequently used as probiotics...

Probiotics are live microbes which when administered in adequate amounts as functional food ingredients confer a health benefit on the host. Their versatility is in terms of their usage which ranges from the humans to the ruminants, pigs and poultry, and also in aquaculture practices. In this review, the microorganisms frequently used as probiot...

Probiotics are live microbes which when administered in adequate amounts as functional food ingredients confer a health benefit on the host. Their versatility is in terms of their usage which ranges from the humans to the ruminants, pigs and poultry, and also in aquaculture practices. In this review, the microorganisms frequently used as probiotics...

High quality DNA extractions are a prerequisite for genetic studies for a variety of plants including Plectranthus forskohlii. Nowadays, there are great number of plant DNA extraction method and commercially available extraction kit are also becoming more and more popular. It appears that different procedures work best for different plant groups. T...

High quality DNA extractions are a prerequisite for genetic studies for a variety of plants including P.forskohlii. Nowadays, there are great number of plant DNA extraction method and commercially available extraction kit are also becoming more and more popular. It appears that different procedures work best for different plant groups. Thus in the...

Objective To screen the antioxidant potential of leaf and stem of the various ecotypes of Brahmi. Methods The medicinally important plant, Bacopa monnieri L (B. monnieri). to analyze the antioxidative enzymes, superoxide dismutase (EC 1.15.1.1) catalase (EC 1.11.1.6) and peroxidases (E.C. 1.11.1.7), and some non-enzymatic antioxidants. Results Th...

Preliminary analysis of flavonoid chromatographic migration profiles of endemic/endangered species of Rauvolfia L from Southern Western Ghats of India were carried out. Paper chromatogram showed maximum separation in the solvent system of forestral. In the paper chromatogram, number of flavonoid spots varied from 9 to 12 in the five taxa studied. T...

Brahmi is a small prostrate herb that grows wild in marshy and damp places near water logs throughout India. The plant has been used since ancient time in folklore and traditional medicine as nervine, cardio-tonic, and diuretic. The plants were collected from18 different geographical regions of south India. RAPD profiles in order to assess diversit...

Rauvolfia has long been regarded as medicinal plant. Leaves of all the reported five species of Rauvolfia from southern Western Ghats were chosen to investigate their antioxidant activities, phytochemicals and nutrient composition. The five species were then screened for their antioxidant potentials using various in vitro models such as total antio...

Phytochemical and antioxidant activity studies of the leaves of Rauovlofia beddomei Hook. (Apocyanaceae) have been carried out. The ethyl acetate fraction of methanol extract yielded five compounds (1 – 5) identified as flavonoids: (1) kaempferol, (2) kaempferol-3-O-D-glucoside, (3) quercetin, (4) apigenin-3-O-D-glucoside, and (5) apigenin-7-O-neoh...

The effect of triadimefon (TDM) a triazole compound on antioxidant potential and eugenol content was studied in Ocimum tenuiflorum L. The plant was subjected to 15 mg/l TDM by soil-drenching on 50th, 70th and 90th days after planting (DAP). Analyses were carried out on 60th, 80th and 100th DAP. Both enzymatic and non-enzymatic antioxidant potential...

To quantify the colchicine (alkaloid) present in Gloriosa superba by High Performance Liquid Chromatography (HPLC) method. Gloriosa superba species are known for their content of colchicine, a valuable alkaloid in the seeds and tubers (corms). HPLC analysis was carried out to confirm the presence of colchicine in Gloriosa superba. Treatment of gibb...

A field experiment was conducted to investigate the effect of growth regulators on growth characteristics such as root length, shoot length, total leaf area, number of inflorescence per plant, number of flower per inflorescence, whole plant fresh weight and whole plant dry weight. Photosynthetic characteristics were also analyzed based on the same...

Environmental stresses (salinity, drought, heat/cold, light and other hostile conditions) may trigger in plants oxidative stress, generating the formation of reactive oxygen species (ROS). These species are partially reduced or activated derivatives of oxygen, comprising both free radical ( \textO2- ,\textOH , \textOH 2 ) ( {\text{O}}_{2}^{\cdot...

The effect of different plant growth regulators (PGR) and elicitor treatments on the alkaloid profile variation of Catharanthus roseus was investigated in the present study. The PGR used were paclobutrazol (PBZ), gibberellic acid (GA3) and Pseudomonas fluorescens elicitors (PF Elicitors). The estimated alkaloids were ajmalicine, catharanthine, tabe...

In the present investigation, different plant growth promoters and retardant were used to determine the anatomical characteristics of Catharanthus roseus. The plant growth promoters used were gibberellic acid (GA ) and Pseudomonas fluorescens elicitors (PF Elicitors) and retardant was paclobutrazol (PBZ). The 3 treatments were given by soil drenchi...

In the present investigation, an attempt was made to study the effect of different triazole compounds viz., triadimefon, paclobutrazol and propiconazole on photosynthetic characteristics of Amorphophallus campanulatus. The results indicated that in Amorphophallus campanulatus the triazole compounds increased the total chlorophyll and carotenoid con...

In this study, Ocimum sanctum plants were treated with paclobutrazol (PBZ) and Abscissic acid (ABA) to analyze the changes in the enzymatic and non-enzymatic antioxidant responses. Non- enzymatic antioxidants like ascorbic acid decreased in the ABA treated plants. But it increased in the PBZ treated plants. Although both PBZ and ABA treatments cons...

The effect of different plat growth regulators like paclobutrazol (PBZ) gibberellic acid and Pseudomonas fluorescens treatments on the non-enzymatic antioxidant components of Catharanthus roseus was investigated in the present study. The treatments were given to plants by soil drenching on 38, 53, 68 and 83 days after planting (DAP). The plants wer...

In the present investigation, changes in individual alkaloid profiles were studied in Catharanthus roseus (L.) G. Don. plants under treatment with Pseudomonas fluorescens. The Pseudomonas fluorescens treatments were given by soil drenching on 38, 53, 68 and 83 days after planting (DAP) by soil drenching. The plants were taken randomly on 45, 60, 75...

The effect of triadimefon was investigated in a medicinal plant, Catharanthus roseus subjected to water deficit stress. The abscisic acid (ABA) level, DNA and RNA contents and activities of ATPase and protease were found varying in different parts of the plants under treatment. Drought treatment increased the ABA level more than twofold in all part...

Triadimefon is a triazole derivative, which have plant growth regulator properties. However, the influential mechanism of triadimefon on medicinal plants like Withania somnifera is not much studied. In the present investigation, the effects triadimefon at 10 mg L−1 on the germination, early seedling growth, photosynthetic pigments, non-enzymatic an...

The ability of triadimefon (TDM), a triazole group of fungicide, to ameliorate salinity stress was studied in Catharanthus roseus (L.) G. Don. plants subjected to sodium chloride (NaCl) treatment. NaCl treatment at 80mM level decreased overall growth of this plant and reduced the chlorophyll contents, protein, antioxidant enzymes such as peroxidase...

Triadimefon, potential fungicide cum plant-growth retardant was used in this study to investigate its effect on the growth and the photosynthetic pigment contents of two varieties of Catharanthus roseus (L.) G. Don. The plants of both varieties were subjected to 15 mg l(-1) triadimefon treatment by soil drenching 30, 45, 60, and 75 days after plant...

The variations in antioxidant potentials and indole alkaloid content were studied in the present investigation, in two varieties (rosea and alba) of Catharanthus roseus, an important herb used in traditional as well as modern medicine, exposed to water deficit stress. The antioxidant and alkaloid profiles were estimated from root, stem, leaf, flowe...

An investigation was carried out to estimate the salt stress ameliorative effect of propiconazole (a triazole group of fungicide cum plant growth regulator) on the NaCl stressed Catharanthus roseus. The experiment was conducted in pots. All the pots were irrigated to the field capacity with ground water up to 30 days after sowing (DAS). The treatme...

Two varieties, rosea and alba, of Catharanthus roseus (L.) G. Don. were screened for their water use efficiency under two watering regimes, viz. 60 and 100% filed capacity in the present study. Drought stress was imposed at 60% filed capacity from 30 to 70 days after sowing, while the control pots were maintained at 100% filed capacity throughout t...

Jaleel C.A., Gopi R., Panneerselvam R. (2008): Biochemical alterations in white yam (Dioscorea rotundata Poir. ) under triazole fungicides: impacts on tuber quality. Czech J. Food Sci., 26: 298-307. An investigation was conducted to find out the effects of two triazole fungicides (triadimefon (TDM) and hexaconazole (HEX) - 15 and 10 mg/l per plant,...

The effect of different plant growth regulators (PGR) and fungicide treatments on the growth characteristics of Catharanthus roseus (L.) G. Don. (Madagascar periwinkle, Family: Apocynaceae) was investigated in the present study. The PGR used were paclobutrazol (PBZ), gibberellic acid (GA ) an d 3 Pseudomonas fluorescens elicitors (PF Elicitors) by...

In a pot culture experiment, the effect of calcium chloride (CaCl2) as an ameliorating agent on sodium chloride (NaCl) stress was studied in Dioscorea rotundata plants. Plants were raised in pots and exposed to salinity stress (80 mM NaCl) with or without 5 mM CaCl2. NaCl-stressed plants showed decreased protein and total sug- ars, and increased fr...

Triadimefon (TDM), a systemic fungicide with non-traditional plant-growth regulator properties, was administered to Catharanthus roseus (L.) G. Don. plants in order to determine its effects on oxidative injury in terms of H2O2 content, lipid peroxidation (LPO), electrolyte leakage (EL), protein and amino acid contents, as well as proline metabolism...

A pot culture experiment was conducted to estimate the drought stress mitigating effect of ketoconazole (KCZ), a fungicide cum plant growth regulator, in Catharanthus roseus plants. The plants under pot culture were subjected to drought stress and drought stress with KCZ from 30 days after sowing (DAS) and regular irrigation was kept as control. An...

The application of triazole fungicides is a common practice in the cultivation of carrot (Daucus carota L.) plants. It is there for seems important to test the changes that are occurring in this food crop under triazoles, the non-traditional plant growth regulators, treatments in order to identify the extent to which it tolerate the fungicide appli...

Changes in antioxidant potentials and indole alkaloid, ajmalicine, production were studied in Catharanthus roseus (L.) G. Don. plants under treatment with gibberellic acid (GA(3)). The GA(3) treatments were given in two ways, foliar spray and soil drenching methods on 30, 45, 60 and 75 days after planting (DAP). The plants were uprooted randomly on...

The effect of plant growth promoting rhizobacteria (PGPR) like Pseudomonas fluorescens on growth parameters and the production of ajmalicine were investigated in Catharanthus roseus under drought stress. The plants under pot culture were subjected to 10, 15 and 20 days interval drought (DID) stress and drought stress with Pseudomonas fluorescens at...

The present investigation was conducted to determine whether CaCl(2) increases Catharanthus roseus drought tolerance and if such tolerance is correlated with changes in oxidative stress, osmoregulation and indole alkaloid accumulation. C. roseus plants were grown under water deficit environments with or without CaCl(2). Drought induced oxidative st...

Catharanthus roseus (L.) G. Don plants were grown in different water regimes in order to study the drought induced osmotic stress and proline (PRO) metabolism, antioxidative enzyme activities and indole alkaloid accumulation. The plants under pot culture were subjected to 10, 15 and 20 days interval drought (DID) stress from 30 days after sowing (D...

The present investigation was carried out with an aim to study the effects of two important fungicides/plant growth regulators, triadimefon (TDM) and hexaconazole (HEX) on white yam (Dioscorea rotundata Poir.). Each plant was treated with 1l of aqueous solution containing 15mgl-1 TDM and 10mgl−1 HEX by soil drenching on 10, 20 and 30days after plan...

An experiment was conducted on Catharanthus roseus to study the effect of seed treatments with native diazotrophs on its seedling growth and antioxidant enzyme activities. The treatments had significant influence on various seedling parameters. There is no significant influence on dry matter production with the diazotrophs, Azospirillum and Azotoba...

The effect of paclobutrazol, a plant growth regulator, on antioxidant defense system was investigated in Catharanthus roseus (L.) G. Don. plants subjected to NaCl stress. The growth parameters were significantly reduced under 80mM NaCl treatment; however, this growth inhibition was less in paclobutrazol-treated (15mgl−1plant−1) plants. The non-enzy...

The ability of triadimefon (TDM), a triazolic fungicide, to alter the biochemical constituents and thereby minimizing the days required for sprouting in white yam (Dioscorea rotundata Poir.) tubers during storage under (30+/-2) degrees C in the dark, was studied. TDM at 20 mg/L was given to tubers by dipping the tubers in treatment solution contain...

In the present investigation, ‘rosea’ and ‘alba’ varieties of Catharanthus roseus (L.) G. Don. seeds were grown with different concentrations (15, 30, 45 and 60 mM) of sodium chloride (NaCl), in order to study the effects of salinity on germination behaviour, seedling vigour (root and shoot length), lipid peroxidation (LPO) and proline metabolism....

Antioxidant responses were analysed in Catharanthus roseus (L.) G.Don. under 0, 50 and 100 mM NaCl in order to investigate the plant's protective mechanisms against long-term salt-induced oxidative stress. The NaCl treatments were repeated in 4 different stages of growth, i.e. 30, 45, 60 and 75 days after sowing (DAS). The plants were uprooted rand...

The effects of triadimefon, a triazole compound on the antioxidant potentials and root alkaloid ajmalicine content were studied in two varieties, rosea and alba of Catharanthus roseus (L.) G. Don., an important medicinal plant. The plants of both the varieties were subjected to 15 mg l−1 triadimefon treatment by soil drenching on 53, 68 and 83 days...

Amorphophallus campanulatus (Elephant Foot Yam) is a rich source of starch, essential amino acids and therefore, used as a vegetable. It is cultivated and utilized in various regions of South India. Triazole compounds are widely used systemic fungicides to control diseases in plants and animals. Many of the triazole compounds have both fungi toxic...

The effects of triadimefon on germination, root length, shoot length, leaf area, sugar content, amylase, ATPase and peroxidase activity of NaCl- stressed pigeon pea seedlings were investigated. Sodium chloride stress decreased germination, inhibited root length, shoot length, leaf area, dry weight and peroxidase activity. However, it increased the...

In the present investigation, three different triazole compounds triadimefon (TDM), paclobutrazol (PBZ) and propiconazole (PCZ) were used to study their effects on the leaf anatomical characteristics of Amorphophallus campanulatus. The triazole treated leaves showed several variations in leaf thickness, number of stomata, stomatal pore length, widt...

In order to meet the ever increasing demand for medicinal plants, for the indigenous systems of medicine as well as for the pharmaceutical industry, some medicinal plants need to be cultivated commercially, but the soil salinity, which is prevalent in many parts of the world, pose serious threat to plant production. So it seems valuable, to test th...

The present investigation was conducted to determine the effect of PBZ and ABA on total phenol contents in different parts of Ocimum sanctum. Paclobutrazol (PBZ) a triazole fungicide, having plant growth regulator (PGR) properties, is reported to inhibit gibberellic acid biosynthesis and increase in abscisic acid and cytokinin contents. So, it nece...