Gloria Evelyn Evans

• PhD
• Fellow at University of Auckland

Purpose This pilot study aimed to develop a methodology characterising the urogenital microbiome as a predictive test in the IVF workup. Methods Using unique custom qPCRs, we tested for the presence of specific microbial species from vaginal samples and First Catch Urines from the male. The test panel included a range of potential urogenital patho...

Study question: Does gene expression of putative endometrial implantation markers vary in expression between menstrual cycles? Summary answer: In fertile women the expression of certain genes exhibits a pattern of stable regulation.which is not affected even when sampled twice in one cycle. What is known already: Successful implantation occurs...

Objective: To characterize the transcriptome of luminal epithelia (LE) of fertile secretory endometria and compare the results with those from glandular epithelia (GE). Design: Endometrial samples were collected at 2 and 7 days after initial blood LH surge in separate menstrual cycles. LE were obtained with the use of laser microdissection. mRNA...

Since the discovery of legionellae, many attempts to improve and expand the spectrum of diagnostic methods have been undertaken, as no one traditional method for the diagnosis of legionella pneumonia is adequate. Amplification of the 16S rRNA region of Legionella spp. using primers designed by Jonas et al., have been available since 1995. Using Q...

Since the discovery of legionellae, many attempts to improve and expand the spectrum of diagnostic methods have been undertaken, as no one traditional method for the diagnosis of legionella pneumonia is adequate. Amplification of the 16S rRNA region of Legionella spp. using primers designed by Jonas et al., have been available since 1995. Using Q...

Since the discovery of legionellae, many attempts to improve and expand the spectrum of diagnostic methods have been undertaken, as no one traditional method for the diagnosis of legionella pneumonia is adequate. Amplification of the 16S rRNA region of Legionella spp. using primers designed by Jonas et al., have been available since 1995. Using Q...

To use contemporary biochemical markers to characterize mRNA/gene expression in the potentially fertile secretory endometrium to confirm its identification based on histological characteristics in order to develop a clinically applicable test. Nine, fertile, cycling Caucasian women were sampled from one IVF clinic. Endometrial samples were collecte...

To map the changes in messenger RNA (mRNA) and protein abundance during the window of implantation in specifically endometrial stromal and glandular epithelial cells obtained using laser microdissection microscopy (LDM). Endometrial samples were collected from two menstrual cycles at 2 and 7 days after first significant rise in blood LH, and separa...

This study compares the Mycoplasma Duo kit for the detection of genital mycoplasmas with conventional culture using A7 differential agar for the detection of Mycoplasma hominis and Ureaplasma urealyticum in clinical samples. Detection of the mycoplasmas is based on the specific metabolic properties of each organism to hydrolyse either arginine or u...

A prospective cohort study was conducted at both Christchurch and Waikato Hospitals in 1998-1999 on patients admitted with Community Acquired Pneumonia (CAP). Disease incidence was ascertained in the specimens of these patients using traditional and a new molecular detection method. The incidence of Chlamydia pneumoniae was obtained by using PCR fo...

To determine the prevalence of hepatitis C virus (HCV) infection in patients attending the Christchurch sexual health centre. Anonymised unlinked serum specimens from 362 patients sequentially attending the sexual health centre were obtained and tested for HCV antibody using the second generation enzyme immunoassay kit (Abbott). Antibody positive s...

To facilitate the diagnosis of Pneumocystis carinii from bronchoalveolar lavage and sputum specimens, we have defined conditions for optimal use of the cytocentrifuge for this purpose. Centrifugation in the cytocentrifuge at 1,200 rpm for 10 min yielded the best recovery of P. carinii. To reliably ensure complete absorption of the fluid specimen fr...

With the dramatic increase in the frequency of Pneumocystis carinii pneumonia associated with human immunodeficiency virus infection, there has been a need for more rapid and less invasive diagnostic techniques. Recent studies have shown that examination of induced sputum can establish the diagnosis of P. carinii pneumonia in about 55 percent of ca...

A combination of three monoclonal antibodies, two prepared against human and one against rat Pneumocystis carinii, was used in an indirect fluorescent-antibody stain (IFA) to diagnose P. carinii in both bronchoalveolar lavage and lung biopsy specimens. This combination of monoclonal antibodies was specific for P. carinii and yielded bright fluoresc...