About
55
Publications
27,991
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,157
Citations
Introduction
Skills and Expertise
Current institution
Additional affiliations
Publications
Publications (55)
This study aimed to evaluate the pulp response to F18 and cobalt-doped F18 bioglass (F18Co) in comparison with calcium hydroxide (CH) after pulp capping. The maxillary first molars of 48 rats were divided into F18, F18Co, CH, and control (no intervention) groups. The pulp was exposed, the materials were placed, and the teeth were capped. After 7 an...
This study aimed to evaluate the pulp response to F18 and cobalt-doped F18 bioglass (F18Co) in comparison with calcium hydroxide (CH) after pulp capping. The maxillary first molars of 48 rats were divided into F18, F18Co, CH, and control (no intervention) groups. The pulp was exposed, the materials were placed, and the teeth were capped. After 7 an...
Objective: This study investigated the acute effects of cocoa flavanol (CF) supplementation on glucose homeostasis, aerobic performance, and lactate concentration in rats with type 1 diabetes mellitus (T1DM), type 2 diabetes mellitus (T2DM), and normoglycemia (NORM).
Materials and methods: The study included 28 male Wistar rats (220-290 g). Inducti...
Global coverage of living coral has declined by half since 1950s. Reef-building species have been severely impacted in this climate crisis scenario, compromising the future of coral reefs. Despite their importance, there is a lack of knowledge regarding the reproductive biology of scleractinian corals. In the present study, we evaluated through ele...
Spermatogenesis is a biological process that occurs inside the seminiferous tubules and results in the production of highly specialized cells, the spermatozoa. The Neotropical rattlesnake Crotalus durissus is a medical-importance snake species that presents a seasonal reproductive pattern. Here we characterized for the first time the spermatogenesi...
Although the order Rodentia does not present a high risk of extinction compared
to mammals as a whole, several families demonstrate high levels of threat and/or
data deficiency, therefore highlighting the need for targeted research and the application of ecological and reproductive data to the development of conservation
actions. The order Rodentia...
Natural products derived from plants can be used as photosensitizers for antimicrobial photodynamic therapy (aPDT) combining key therapeutic strategies for tissue repair while controlling microorganisms' growth. We investigated a standardized extract of pequi peels ( Caryocar brasiliense Cambess) as a brownish natural photosensitizer for aPDT using...
Previously, by employing 3D organotypic tissue culture and patient-derived xenograft (PDX) model, oral myxoma response to a MAPK/MEK inhibitor was observed. Gross examination of the tumour fragments obtained after 55 days of PDX grafting revealed increased capsule vascularization. Microscopic analyses showed blood capillaries intermixed with myxoma...
Background:
Central giant cell granulomas (CGCG) of the jaws are osteolytic lesions that may behave aggressively and respond poorly to surgery. Microscopically, in addition to giant cells, there is a mononuclear cell population composed of macrophage/monocytic cells and spindle-shaped cells of mesenchymal origin. 72% of these tumours harbour mutua...
We aimed to investigate the combined effects of aerobic exercise (EXE) and cocoa flavanol (COCOA) supplementation on performance, metabolic parameters, and inflammatory and lipid profiles in obese insulin-resistant rats. Therefore, 32 male Wistar rats (230–250 g) were fed a high-fat diet and a fructose-rich beverage for 30 days to induce insulin re...
Objective: The aim of this study was to verify the effects of consumption of a high-fat diet (HFD) combined with fructose-rich beverages (FRT) in promoting metabolic and physiologic changes associated with insulin resistance. Methods: Thirty-two male Wistar rats (250 § 10 g) were randomly allocated into four groups (n = 8) that received either a st...
Yellowish myotis is a Neotropical vespertilionid bat that presents a seasonal reproduction. The sperm is produced in the Mature stage, stored in the Regressed stage and released in the Rest stage (mating period). Aiming to understand, for the first time, the relationship between testis and epididymis physiology in yellowish myotis reproduction, the...
The sexual segment of the kidney (SSK) is a hypertrophied region of the nephron, which occurs in males of most squamate species that have been investigated, at least, during the active season. Many studies have shown that the SSK has a seasonal secretory cycle that could be correlated to the mating season, testicular activity and androgen synthesis...
Objective
: To verify the effects of consumption of a high-fat diet (HFD) combined with fructose-rich beverages (FRT) in promoting metabolic and physiological changes associated with insulin resistance.
Methods
: Thirty-two male Wistar rats (250 ± 10 g) were randomly allocated into four groups (n = 8) that received either a standard diet (CON), HF...
The authors regret that in our published paper entitled “Comparative testis structure and function in three representative mice strains.”
Mice are widely used as experimental models due to several positive characteristics and in particular their suitability for studies involving molecular biology and transgenesis. Despite the large number of mice strains currently available, the literature regarding their basic reproductive biology is still relatively scarce. Herein, we comparatively...
In this study we prepared annatto-loaded cellulose acetate nanofiber scaffolds and evaluated both in vitro cytotoxicity and potential for wound healing in a rat model. Annatto extract, which has been used to accelerate wound healing, was added to cellulose acetate polymer and the resulting material was used to produce nanofiber scaffolds via electr...
Objective
Odontogenic myxoma (OM) occasionally responds poorly to surgical treatment. The MAPK pathway is constitutively activated in several neoplasms and we aimed to test if the MAPK pathway is activated in OM, in order to pave the way for an alternative therapy for aggressive and recurrent cases.
Materials and methods
The immunoexpression of ph...
Mutations in Foxn1 and Prkdc genes lead to nude and severe combined immunodeficiency (scid) phenotypes, respectively. Besides being immunodeficient, previous reports have shown that nude mice have lower gonadotropins and testosterone levels, while scid mice present increased pachytene spermatocyte (PS) apoptosis. Therefore, these specific features...
PURPOSE
Testicular torsion is an urological emergency and an important cause of male infertility. Recent studies have shown that not only ischemia, but also the restoration of blood flow creates a deleterious situation for the testis. The goal of this work was to evaluate the role of systemic epithelial growth factor application and its association...
Aims:
To investigate the effects of moderate aerobic physical training on cardiac function and morphology as well as on the levels of glial cell-derived neurotrophic factor (GDNF), nerve growth factor (NGF) and brain derived neurotrophic factor (BDNF) of animals infected with the Y strain of Trypanosoma cruzi.
Main methods:
Twenty-eight male C57...
Ameloblastic Fibrodentinoma (AFD) is a benign odontogenic tumour of unclear pathogenesis. It is usually diagnosed before the age of 20 years and treatment is conservative surgery, with occasional segmental resection (Philipsen et al., 1997, Chrcanovic & Gomez, 2017). AFD can perforate cortical bone and recur.
AFD is composed of odontogenic ectomese...
Zika virus (ZIKV) is a teratogenic mosquito-borne flavivirus which can be sexually transmitted from man to woman. High viral loads and prolonged viral shedding in semen suggest that ZIKV replicates within the human male genital tract, but its target organs are unknown. Using ex vivo infection of organotypic cultures, we demonstrated here that ZIKV...
Collared peccaries (Tayassu tajacu) present a unique testis cytoarchitecture, where Leydig cells (LC) are mainly located in cords around the seminiferous tubules (ST) lobes. This peculiar arrangement is very useful to better investigate and understand the role of LC in spermatogonial stem cells (SSCs) biology and niche. Recent studies from our labo...
In recent decades, infertility has been considered a major widespread public health issue of very high concern. Currently, almost 50% of infertility cases are due to male factors, including semen disorders, obstructions, cryptorchidism, varicocele and testicular failures, which can occur due to malfunctions in both somatic and germ cells. In this c...
The establishment of proper conditions for spermatogonial stem cells (SSCs) cryopreservation and storage represents an important biotechnological approach for the preservation of the genetic stock of valuable animals. This study demonstrates the effects of different cryopreservation protocols on the survival rates and phenotypic expression of SSCs...
The objective was to evaluate the histomorphometric characteristics of the testis and ovaries of pig fetuses at different gestational ages, as well as their correlation with some fetus measurements. Forty-four fetuses were separated for gender (male and female) and gestational age (50, 80 and 106days of gestation). After slaughter, fetuses had thei...
In broiler breeder roosters, the weight of the testes is positively associated with daily sperm production and fertility. In birds, the testes are located in the coelomic cavity, not being accessible to reproductive evaluation as mammalian testes. The reproductive evaluation of roosters is based on phenotypic traits. Any changes on testes will be r...
Several studies have demonstrated that a high-fructose (FRUC) diet induces metabolic and haemodynamic abnormalities, known as the metabolic syndrome, which are characterised by obesity, glucose intolerance, insulin resistance, dyslipidaemia and hypertension. In this study, the effect of a FRUC diet (60 % fructose) for 8 weeks on the metabolism of l...
• Phthalate esters can leach from plastic products leading to environmental exposure.• In fetal rats phthalate exposure causes abnormal development of the male reproductive tract.• Development of immature primate testis exposed to phthalates was studied after xenografting to mouse hosts.• Long-term exposure to phthalates reduced steroidogenic gene...
Open in 2013, the Interactive Space of Life Sciences is located in the city of Belo Horizonte, Minas Gerais, Brazil. Integrated to the Museum of Natural Sciences and Botanical Garden of the Universidade Federal de Minas Gerais, it represents an innovative environment where technology and art are combined with conventional education tools such as an...
Because the collared peccary (Tayassu tajacu) has a peculiar Leydig cell cytoarchitecture, this species represents a unique mammalian model for investigating testis function. Taking advantage of the well-established and very useful testis xenograft technique, in the present study testis tissue and testicular cell suspensions from immature collared...
Morphometry is a classical quantitative method often used in biology to provide a data basis for functional interpretations/interactions of a particular organ or system. Herein we took advantage of this valuable approach to evaluate the spermatogonial stem cell niche using the horse testis and immunocytochemical localization of GFRA1 [glial cell li...
Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis and are located in a highly dynamic microenvironment called "niche" that influences all aspects of stem cell function, including homing, self-renewal and differentiation. Several studies have recently identified specific proteins that regulate the fate of SSCs. These studies als...
Spermatogonial stem cells (SSCs) are located in a particular environment called the ‘:niche’ that is controlled by the basement membrane, key testis somatic cells, and factors originating from the vascular network. Although crucial for SSC physiology, the niche is still poorly understood, particularly in non-model vertebrates where the testis cytoa...
Phthalic acid esters, such as dibutyl phthalate (DBP) are widely used as plasticizers in many daily-used products from which they leach into the environment. In rodents, gestational exposure implicated phthalates in the testicular dysgenesis syndrome. However, little is known regarding the effects of phthalate exposure on postnatal testis develomen...
Nitric oxide (NO) plays crucial roles in several physiological and pathological conditions. The iNOS isoform produces high levels of NO independent of intracellular calcium and, in the testis, which is expressed in Sertoli (SC), Leydig (LC) and germ cells. The testicular roles of NO are unclear, but it can inhibit LC testosterone production. Our ai...
Nitric oxide (NO) is produced via oxidation of l-arginine by nitric oxide synthases (NOSs), and is known as inducible (iNOS), neuronal, endothelial or testis-specific. Suggesting important functions for NOS in the normal rat and mouse testis, iNOS is reported to be constitutively expressed in Leydig cells (LC), Sertoli cells (SC) and germ cells. In...
With the exception of the domestic cat, all members of the family Felidae are considered either endangered or threatened. Although not yet used for this purpose, spermatogonial stem cell (SSC) transplantation has a high potential to preserve the genetic stock of endangered species. However, this technique has not previously been established in feli...
Although seminiferous tubule maturation in horses begins in the central area of the testis, this process is thought to occur randomly throughout the testis in most mammals. Studies in our laboratory revealed that the establishment of spermatogenesis may not be a synchronous event in the testicular parenchyma of pigs. The objectives of the present s...
Testis structure and function in dogs are relatively poorly investigated. The aim of the present study was to carry out a comparative investigation of the stages of the seminiferous epithelium cycle and its duration in different breeds of dog. Fifty-six sexually mature dogs (mongrel, n = 12; pinscher, n = 12; beagle, n = 5; American pit bull, n = 9...
Xenografting of immature mammalian testis tissue into mice can accelerate sperm production. To determine whether this shortened time to sperm production is because of reduced length of the spermatogenic cycle, we applied bromodeoxyuridine (BrdU) incorporation to analyze the spermatogenic cycle in porcine and ovine testis xenografts. Small testis fr...
Grafting of immature testis tissue from different mammalian donor species into mouse hosts results in production of spermatozoa from the donor species. Xenografting of testis tissue from rhesus monkeys, pigs, and sheep accelerates sperm production. To determine whether this shortened time to sperm production is due to the reduced spermatogenic cycl...
Starting from the period of testis differentiation, the Sertoli cell plays a pivotal role in the development of a functional testis. FSH is the major mitotic factor for Sertoli cells. Because the supporting capacity of Sertoli cells is relatively fixed for each species, their total number per testis, established just before puberty (approximately 4...
