Giuseppe Stradaioli

University of Udine | UNIUD · Department of Agricultural, Food, Environmental and Animal Sciences

Pollution due to microplastics and nanoplastics is one of the major environmental issues of the last decade and represents a growing threat to human and animal health. In aquatic species, there is a large amount of information regarding the perturbation of marine organisms; instead, there are only a few studies focusing on the pathophysiological co...

The aim of this study was to estimate genetic parameters and investigate the genomic background of scrotal circumference and semen parameters in the Italian Simmental bulls. Scrotal circumference, number of normal spermatozoa, ejaculate volume, spermatozoa motility, and total spermatozoa were measured on 622 bulls, of which 603 had genotypes for 42...

The objectives of the study aimed to evaluate the effect of weather conditions and scrotal circumference (SC) on standard semen characteristics of Italian Simmental young bulls (n = 577), all raised in the same performance station and sampled by the artificial vagina (AV) method. Considering the increasing SC, the results showed a significant incre...

Dystocia, defined as prolonged or difficult birth, negatively affects the dam and calf survival, health and welfare together with the economy of the farm. The main income in beef industry is represented by calves to be grown for meat production. However, stillbirth and dam mortality due to dystocia represent an economic and welfare issue and even i...

Cattle farming is facing an increase in number of animals that farmers must care for, together with decreasing time for observation of the single animal. Remote monitoring systems are needed in order to optimize workload and animal welfare. Where the presence of personnel is constant, for example in dairy farms with great number of lactating cows o...

One critical point of dairy farm management is calving and neonatal first care. Timely calving assistance is associated with the reduction of calf mortality and postpartum uterine disease, and with improved fertility in dairy cattle. This study aimed to evaluate the performance and profitability of an intravaginal sensor for the prediction of stage...

Intrauterine laparoscopic insemination is diffuse in sheep breeding, yet requires veterinary expertise and expensive equipment. Our aim was to evaluate the time required, reproductive outcome and post-operative complications of mini invasive laparotomic intrauterine insemination, as an alternative approach to laparoscopy. A total of 115 Sopravvissa...

The objective of the present study was to evaluate the effect of 24 hours’ fasting prior to semen collection by electroejaculation on behavioral responses, volume of rectal fecal content, bladder size, penis protrusion, erection, ejaculation stimuli, and ejaculate parameters in young Simmental bulls. Twenty-two Simmental beef bulls with an age of 1...

Buffalo breeding is common in Southern Italy. Dystocia compromises dam’s and newborn health and welfare. Difficult parturition could be solved through prompt calving assistance, even if the identification of the beginning of delivery is challenging. Herein we aimed to evaluate a remote calving alarm system in 15 Mediterranean buffalo heifers. An in...

The aim of the study was to evaluate the net return of the implementation of a remote calving monitoring system for obstetrical and neonatal assistance on the herd economy in a dairy farm model. A total of 680 parturitions over a 7-yr period were evaluated. Age at first calving was restricted from 23 to 27 mo for primiparous cows to be included. Am...

Background: Despite being one of the major causes of infertility in mares, the mechanisms responsible for equine endometrosis are still unclear and controversial. In the last few years, many investigations focused on local immune response modulation. Since it is generally accepted that endometrial fibrosis increases with age, we hypothesize that o...

Signs of dysuria in rams are usually associated with urethral obstruction caused by calculi, especially in castrated males, while penile trauma is rarely reported as a cause of dysuria. In this paper, a case of a 4-year-old, 65 kg bodyweight, Sardinian intact ram referred to the Teaching Veterinary Hospital (OVUD) at the Department of Veterinary Me...

The transcriptome associated with the ability of oocytes to support embryogenesis and development remains unclear. The aim of this study was to compare the mRNA expression pattern between oocytes collected from fertile heifers (H) and repeat breeders (RB) using RNAseq. Oocytes were collected by ovum pick-up from Italian Simmental (IS) and Holstein...

The experiment was aimed to evaluate the usefulness of hair cortisol concentrations (HCC) in revealing the activation of the hypothalamic-pituitary-adrenal (HPA) axis due to the passage of young bulls from genetic rearing stations to artificial insemination (AI) centre and the influence of this passage on hair testosterone concentrations (HTC). Hai...

Traditional superovulation protocols that include multiple gonadotropin treatments are time-consuming and labor intensive. These protocols require multiple handling and restraining of embryo donors. This will likely increase the risks of injuries in both animals and humans and induce stress that may lead to a reduced superovulatory response. These...

Background Whether the measurement of cortisol in dairy cows can be used as a biomarker of adverse environmental or pathophysiological conditions is still under of scientific debate. In these situations, several systems mainly the hypothalamic-pituitary-adrenal axis, the autonomic nervous system, and the immune system are recruited to reestablish h...

Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is a selenoprotein, which protects biomembranes from oxidative damages, and it also accounts for almost the entire selenium content of mammalian testis. The present investigation was performed to localise PHGPx in the testis and in epididymal and ejaculated spermatozoa of the bull by using l...

Proper calving management of dairy herds is a crucial aspect of the bovine life cycle, as it has profound effects on calf viability and on the post-partum course of the dam. The objectives of this study were to monitor the calving process through the use of a remote alarm system and to determine the impact of prompt emergency obstetric procedures i...

Maternal transcripts are accumulated during oocyte growth and drive early embryonic development; therefore, their characterisation is a relevant factor for predicting fertility. DNA microarrays have been the method of choice for transcriptional profiling, but this method has some limitations when applied to domestic species because it relies upon e...

Persistent increase on cortisol concentrations due to chronic stress can have detrimental effects on reproductive performance of animals. Blood cortisol concentrations have been used to investigate the stressmediated influence of the adrenocortical system on the hypothalamic-pituitary-gonadal (HPG) axis. To date, cortisol assessment in hair samples...

Cystic ovarian disease represent an unsolved problem in bovine reproduction. Despite the huge amount of literature, the exact pathogenesis of the disease is still not completely clear and therefore also the choice of treatment is still debated. The present study was aimed to compare the effective efficacy of the three most common drugs used for ova...

The aim of this study was to establish baseline ultrasound data concerning the mule conceptus during gestation. Ten multiparous Trotter mares were artificially inseminated with chilled semen from an Amiatino jack donkey. Daily transrectal ultrasonography was carried out from the day of ovulation until Day 50 of gestation to determine the following:...

In dairy farm management economic losses resulting from cystic ovarian degeneration are well known. In spite of this, neither the definition nor the aetiopathology of ovarian cysts are clear and agreed upon. Also the usual classification in luteal and follicular cysts, requiring ultrasound examination together with assessment of P4 to be accurate,...

Jan Govaere, Katrien Martens and Aart de Kruif, together with their talented collaborators at the Department of Reproduction, Obstetrics and Herd Health at Ghent university (in cooperation with Utrecht university, Ohio State University and the University of Nantes), have produced an educational DVD that is intended mainly at students, vets and teac...

Recent advances in understanding the control of the mammary cell population now offer new insights to understand the decline in milk yield of dairy animals, which has long been a biological conundrum for the mammary biologists. Evidence indicates that change in mammary cell number is the result of an imbalance between cell proliferation and cell re...

Abstract not available.

The aim of this study was to develop a system for the monitoring of calving to both reduce perinatal mortality and improve dairy cow fertility by preventing the majority of post-partum reproductive pathologies. Eighty dairy cows were assigned to the protocol of calving monitoring using GSM (Global System for Mobile Communications) technology. The a...

The imbalance between reactive oxygen species (ROS) and antioxidant capacity of the organism leads to a condition of oxidative stress (Urso and Clarkson, 2003). Studies in humans and laboratory animals have reported that oxidative stress is related to some common degenerative diseases, such as cancer and cardiovascular pathologies (Pellegrini et al...

Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is a selenoperoxidase accounting for most of the selenium content in mammalian testis, which has been found to be linked to fertility in humans. In this study, we addressed the issue whether PHGPx content in spermatozoa could be a predictive index of fertilization capacity for sire selection...

The effect of a rapid variation of dietary starch and fat on metabolic and oxidative status in lactating ewes was investigated. Fifteen sheep at the end of pregnancy were assigned to three experimental groups and fed until 32 days from lambing a complete diet (22% starch and 3% fat on DM basis). At day 33, a group of ewes continued to receive the c...

Stress activates the hypothalamo–pituitary–adrenal axis leading to enhanced glucocorticoid secretion and concurrently disrupts ovarian cycle. Plant polyphenols are known to posses antioxidant and anti-inflammatory proprieties. This could be of interest for ovarian cycle when stressing conditions lead to progesterone enhancement and hamper normal re...

Gastrin (GAS) and insulin (INS) play a significant role in the regulation of feed intake, as well as in the homeorhetic adaptations to different physiological stages, nutrient supply and requirements. The aim of the study was to verify if plasma GAS and INS concentrations in adult does are affected by the physiological state, change in response to...

The objectives of the present study were (i) to establish the mean value of scrotal circumference (SC), sperm motility, concentration and morphology at 13+/-1 months of age for Chianina, Marchigiana, and Romagnola breeds and (ii) to assign Italian beef bulls at the end of a growth performance test to a potential breeder category by applying the gui...

Growing evidence suggests that among the causes which deteriorate qualitative and functional characteristics of semen after freezing and thawing, there are those linked to oxidative stress and decrease detoxification of antioxidants present in the cells and seminal plasma. Overall, cryopreservation reduces the spermatozoal GSH content. In the prese...

Granulosa-theca cell tumors (GTCTs) are able to secrete variable amounts of sex steroids and immunoreactive inhibin (ir-INH). Although the pituitary appears to be affected by the presence of a GTCT, pituitary responsiveness to exogenous GnRH has not been examined. The aims of the present study were to: (i) assess the plasma hormone concentrations o...

The biochemical and molecular effects of tomato pomace (1.3% lycopene) or grape skin (87% polyphenols) supplementation for the control of hand-on induced stress in sheep were investigated. Twenty ewes were fed with a same basal diet for a preliminary period of 30 days and thus assigned to five groups: control group without any antioxidant (CTR); α-...

The study of cell turnover of mammary gland helps to gain understanding of the subtle modulation that largely determines milk production. To evaluate the effect of diet composition on cell turnover of mammary gland, pluriparous sheep were allotted to three experimental groups and fed from day 33 after lambing a control diet (BD), a high starch (HS)...

Several Mycoplasma species may adversely affect bovine spermatozoa viability and embryo development. Mycoplasma mycoides ssp. mycoides large-colony (LC) has been isolated from naturally aborted bovine fetuses and from bull semen. The objective of this study was to evaluate whether M. mycoides ssp. mycoides LC contaminated bovine ejaculates could (i...

Oxidative stress may be important in early lactation cows due to high metabolic demands. Modifying dietary starch may alter glucose utilization, modify oxidative metabolism and, consequently, increase oxidative stress. To test this hypothesis, early lactation primiparous cows underwent a short-term dietary starch restriction followed by realimentat...

The effect of orally administered l-carnitine on the quality of semen obtained from stallions with different semen qualities was investigated. Four stallions with proven fertility (high motility group, HM) and with normal seminal characteristics (>50% progressive motility and > 80 x 10(6) spermatozoa/ml), and four questionable breeders (low motilit...

Dal 1998 al giugno del 2003 sono state registrate in Friuli Venezia Giulia 38 predazioni attribuibili alla lince. Nel presente studio sono stati considerati solamente 23 casi, a carico di capriolo, cervo e capra attribuibili alla lince con un grado di sicurezza Q2 (segni indiretti rilevati da personale qualificato formato attraverso corsi specifici...

Nitrate (NO_ 3) and nitrite (NO_ 2) can be ingested with water and feeds and NO_ 2 can be produced from NO_ 3 by rumen and intestinal microbes. Moreover,NO_ 3 and NO_ 2 are endogenously produced by cells of various tissues from nitric oxide (NO) and arginine (Passi, 2000). Toxicity of NO_ 3 is casued by the reduction to NO_ 2 in the rumen environme...

Ce travail s'intéresse à la peroxydation des lipides membranaires après conservation et cryoconservation des spermatozoïdes de bouc, d'étalon, de dindon, et aux altérations de l'ADN du noyau chez le spermatozoïde de truite arc-en-ciel après cryoconservation.

Granulosa-theca cell tumours represent 2.5 to 4.4% of equine neoplasms, which require elective surgical approaches in order to restore fertility The retrospective study reported herein utilized the multidisciplinary approach to describe i) clinical findings and diagnostic features, ii) surgical approach, iii) and reproductive follow-up in seven gra...

The aim of the study was to compare the effects of heat stress and feed restriction on hormonal secretion (progesterone, oestradiol-17β, luteinizing hormone, follicle-stimulating hormone, prolactin and cortisol) in Holstein heifers. Ten pubertal heifers were divided into two groups of five (A and B) and housed in climatic chambers. After a pre-expe...

The reproductive characteristics and seminal carnitine and acetylcarnitine content as well as carnitine acetyltransferase activity of young Maremmano stallions (n=25) are reported. The stallions were subjected to semen collection in November and January; in each trial two ejaculates were collected 1h apart. The total motile morphologically normal s...

The testes of prepubertal and adult horses were investigated using 10 horseradish peroxidase conjugated lectins combined with sialidase digestion and potassium hydroxide treatment, to localise the oligosaccharide sequences of glycoconjugates during spermatid maturation. In adult animals, the lectins showed a variable affinity for spermatids and Ser...

The objective of this study was to investigate the effect of alpha-tocopherol deprivation on mammary gland involution and apoptosis in sheep. Two groups of four single lamb ewes were used. The control group received 100 mg/d of RRR-alpha-tocopherol supplementation and the experimental group received no vitamin E supplementation. After 3 mo of suckl...

In this study, by using the polymerase chain reaction (PCR) diagnosis for the detection and identification of Mycoplasma, we investigated mycoplasmas contaminating the semen of yearling bulls affected by seminal vesiculitis. The bulls presented neither subclinical nor clinical contagious bovine pleuropneumonia signs and the complement fixation test...

Types and distribution patterns of glycoconjugates in antral ovarian follicles were investigated in the buffalo, using periodic-acid Schiff (PAS), high iron diamine (HID), low ion diamine (LID) and lectin histochemical staining methods. HID and LID staining procedures were preceded in some cases by digestion with testicular hyaluronidase, Streptomy...

This study was undertaken to determine the lectin affinity of the extratesticular rete testis and ductuli efferentes epithelial cells in adult and prepubertal horses, using ten different lectin horseradish peroxidase conjugates: Con-A, LCA, WGA, GSA-II, SBA, PNA, RCA-I, DBA, UEA-I, and LTA. In some cases, treatments with sialidase and KOH preceded...

This paper describes an approach for studying the structure of glycoconjugates found in the principal cells lining the epididymal duct in adult and prepubertal horses, using ten different lectin horseradish conjugates: Con-A, LCA, WGA, GSA-II, SBA, PNA, RCA-I, DBA, UEA-I, and LTA. Saponification and sialidase procedures, followed by lectin binding,...

This study was undertaken to determine the pattern of saccharides of zona pellucida glycoconjugates in immature, in vitro matured and tubal unfertilized bovine oocytes using lectin horseradish peroxidase conjugates. Immature and in vitro matured oocytes were obtained from ovaries of recently slaughtered Chianina heifers. Oocytes present in follicle...

The objective of this study was to characterize the glycoconjugates present in the zona pellucida of the follicular oocytes in sheep, goats and pigs. The zona pellucida was stained with periodic acid-Schiff, low iron diamine, high iron diamine, and nine different lectin horseradish conjugates: Con-A, SBA, DBA, PNA, RCA-I, GSA-II, WGA, LTA and UEA-I...