Giuseppe Palleschi

Giuseppe Palleschi
University of Rome Tor Vergata | UNIROMA2 · Dipartimento di Scinze e Tecnologie Chimiche

About

382
Publications
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Publications

Publications (382)
Article
An Enzyme Linked ImmunoMagnetic Electrochemical assay (ELIME) was developed for the detection of the hepatitis A virus (HAV). This system is based on the use of new polydopamine-modified magnetic nanobeads as solid support for the immunochemical chain, and an array of 8 screen-printed electrodes as a sensing platform. Enzymatic-by-product is quickl...
Article
A novel amperometric algae-based biosensor was developed for the detection of photosynthetic herbicides in river water. The green photosynthetic algae Chlamydomonas reinhardtii was immobilized on carbon black modified screen-printed electrodes, exploiting carbon black as smart nanomaterial to monitor changes in algae oxygen evolution during the pho...
Article
In this study we reported the development of a paper-based algal biosensor for the optical detection of nanoencapsulated-atrazine, a forefront nanoformulated herbicide with a high effective post-emergence herbicidal activity. In particular, the unicellular green photosynthetic algae Chlamydomonas reinhardtii was immobilised on a paper substrate soa...
Article
Campylobacter is an important cause of acute bacterial disease in human worldwide. Within the genus Campylobacter, C. jejuni and C. coli are the two species responsible for over 95% of human infections. The purpose of this work is the development of an Enzyme-Linked-Immuno-Magnetic-Electrochemical (ELIME) assay for a simple and rapid detection of b...
Article
Enzymes are biological catalysts widely exploited in analytical chemistry thanks to their bio‐catalytic activity. For instance, they can be used as bio‐components for sensing applications, being environmentally friendly, inexpensive, and easy to use functional biomaterials. Nowadays, the latest advancements in nanotechnology allow enzymes to be com...
Article
Polybrominated diphenyl ethers (PBDEs) are a type of flame retardants which are currently banned in EU and USA due their hazardousness for humans and mammals. However, these compounds were highly used during more than 30 years and still persist in the environment since they are resistant to degradation. Herein we present a biosensor for the detecti...
Article
The increasing demand by citizens and environmental organization for the protection, preservation, and possible restoration of the marine environment has made seawater protection one of the urgent priorities of the European Union. In this contest, extensive monitoring and surveillance are required to correctly assess the current status of marine en...
Article
Herein, we report an impedimetric DNA-based aptamer sensor for a single-step detection of B. anthracis spore simulant (B. cereus spore). Specifically, we designed a miniaturized label-free aptasensor for B. cereus spores based on a gold screen-printed electrode functionalized with B. cereus spores-binding aptamer (BAS-6R). Several parameters were o...
Article
Herein, we propose the first three-dimensional origami paper-based device for the detection of several classes of pesticides by combining different enzyme-inhibition biosensors. This device was developed by integrating two different office paper-based screen-printed electrodes and multiple filter paper-based pads to load enzymes and enzymatic subst...
Article
Bioassays based on enzyme inhibition are analytical tools widely employed for inhibitor analysis. Beside the conventional analytical techniques such as chromatography and mass spectrometry, these bioassays are cost-effective, easy to use, and suitable for in situ measurement but they are often characterised by a quite narrow linear range. Herein, w...
Article
Disposable non-invasive and compatible real time monitoring tool was developed in order to follow the cleaning process of paper artwork directly in situ. This tool was based on a biocompatible cleaning hydrogel coupled with flow electrochemical diagnostic tool, suitable to verify in situ and in a simple way the assessment of degradation of artwork...
Article
Cortisol is a steroidal hormone and an important stress marker. Free serum cortisol concentration has been identified to correlate well with free salivary cortisol. In this present work an electrochemical immunosensor was developed to determine cortisol concentration within the physiological concentration range found in human saliva. The immunosens...
Article
Full-text available
A novel NiO nanoparticles modified screen printed electrode (SPE) was developed that couples the easy-to-use advantages of this technology to the pseudocapacitance properties of NiO. In order to find the conditions for achieving the best performing modified SPEs, NiO nanoparticles were purposely synthesized via a microwave assisted procedure follow...
Article
Full-text available
This work deals with the use of silver nanoparticles, to produce an electrochemical enzymeless probe. Silver nanoparticles play the dual role of markers for electrochemical measurements and support of an immunological chain for immunoglobulin (IgG). AgNPs are synthesized by modifying the Brust method in terms of time and amount of reagents. The int...
Article
Chemical warfare agents (CWAs) have become a crucial concern of the 20th century in the safety vision of the society and the environment, triggering a growing awareness for early alarm in case of terroristic attacks as well as preventing contamination. Herein, we present the development of a whole-cell optical bioassay for the detection of CWA simu...
Article
Intense farming represents one of the main sources causing detriments to vital resources as lands and water, due to unsustainable agricultural practices and the resulting environmental pollution. Furthermore, the increasing world population and the impact of climate change contribute to worsen these constraints. To these regards, several attempts h...
Article
Herein, we report a first example of a rapid and easy to use paper-based printed sensor embedded in a 3D printing device to measure the butyrylcholinesterase activity in serum samples. The innovative device relies on a paper-based sensor fabricated using wax printing and screen-printing technologies, combined with a printed holder made by stereolit...
Article
The recent goal of sustainability in analytical chemistry has boosted the development of eco-designed analytical tools to deliver fast and cost-effective analysis with low economic and environmental impact. Due to the recent focus in sustainability, we report the use of low-cost filter paper as a sustainable material to print silver electrodes and...
Article
Full-text available
Salmonella has represented the most common and primary cause of food poisoning in many countries for at least over 100 years. Its detection is still primarily based on traditional microbiological culture methods which are labor-intensive, extremely time consuming, and not suitable for testing a large number of samples. Accordingly, great efforts to...
Article
In this paper screen printed electrodes modified with NiO nanoparticles were developed and characterized. NiO nanoparticles were purposely synthesized by a microwave-assisted procedure followed by calcination either at 400 °C or 600 °C and characterized by X-ray diffraction and Scanning Electron Microscopy. Screen printed electrodes were modified b...
Article
The detection of Salmonella according to EC regulation is still primarily based on traditional microbiological culture methods that may take several days to be completed. The purpose of this work is to demonstrate the applicability of an Enzyme-Linked-Immuno-Magnetic-Electrochemical (ELIME) assay, recently developed by our research group for the de...
Article
Through the years, scientists have developed cutting-edge technologies to make (bio)sensors more convenient for environmental analytical purposes. Technological advancements in the fields of material science, rational design, microfluidics, and sensor printing, have radically shaped biosensor technology, which is even more evident in the continuous...
Article
Paper-based microfluidic devices are gaining large popularity because of their uncontested advantages of simplicity, cost-effectiveness, limited necessity of laboratory infrastructure and skilled personnel. Moreover, these devices require only small volumes of reagents and samples, provide rapid analysis, and are portable and disposable. Their comb...
Chapter
A paramount research is focused nowadays on agrifood analysis, to ensure the commercialisation of safe products that meet the requirements of consumer perception. Food manufacturing processes might be threatened by contamination or lack of quality at each stage of the food supply chain while growing, processing, packaging, or distributing. Therefor...
Article
We report a screen-printed electrode (SPE) modified with a dispersion of carbon black (CB) and chitosan by drop casting. A cyclic voltammetry technique towards ferricyanide, caffeic acid, hydroquinone, and thiocholine was performed and an improvement of the electrochemical response with respect to bare SPE as well as SPE modified only with chitosan...
Article
Lactate monitoring is an important issue in the food industry, in clinical diagnosis and sport medicine. Biosensors that can quantify lactic acid in biological samples are extremely attractive because of their advantages such as simplicity, ease of miniaturization, high sensitivity and low-cost. The proposed biosensor is based on a screen-printed e...
Article
Paper has been used as writing and drawing support for thousands of years. The conservation of paper artworks plays a fundamental role in the field of our cultural heritage. Moreover, restoration of paper artworks is difficult due to their inherent fragility, the presence of many components and their degradation state. Among the factors that may co...
Chapter
Full-text available
Since the introduction of modern CWAs at the beginning of 20th century, there has been a continuous interest in the development of robust and reliable analytical tools for the detection of these agents, to provide early alarm in case of terroristic attacks, as well as to monitor their presence in the environment and prevent contamination. Neverthel...
Article
This review addresses recent advances in the development of screen-printed electrode based biosensors modified with different nanomaterials such as carbon nanotubes, graphene, metallic nanoparticles as gold, silver and magnetic nanoparticles, and mediator nanoparticles (Prussian Blue, Cobalt Phthalocyanine, etc.), coupled with biological recognitio...
Article
Full-text available
Wet cleaning of ancient papers is one of the most delicate and important steps in a restoration process. It allows to improve the optical quality of a graphic work as the removal of pollution and of organic substances resulting from cellulose degradation. Nevertheless, washing by immersion – the traditional process – usually involves a substantial...
Article
Paper manuscripts are among the most valuable historical artifacts, but they are also very fragile. Furthermore, they were often strengthened by gluing external materials (like wooden or cardboard lining paper) to the original piece. The glues induced structural transformations that accelerate the degradation processes of the artwork itself. In thi...
Article
Full-text available
A reliable, low-cost and easy-to-use ELIME (Enzyme-Linked-Immuno-Magnetic-Electrochemical) assay for detection of Salmonella enterica in irrigation water is presented. Magnetic beads (MBs), coupled to a strip of eight-magnetized screen-printed electrodes localized at the bottom of eight wells (8-well/SPE strip), effectively supported a sandwich imm...
Conference Paper
Full-text available
The technological application of the  rays is used in various fields: the sterilization of materials and equipment for medical use pharmaceutical and cosmetics, but also for wooden musical instruments. The purpose of irradiating the wood is to improve the "shelf-life", lowering the microbial charge of deterioration. In general, the sterilization b...
Conference Paper
Full-text available
Hepatitis A virus (HAV) causes an acute hepatitis associated with a significant morbidity and occasional mortality. Outbreaks of waterborne diseases are certainly underestimated due to the lack of adequate programs for the epidemiological surveillance. Current legislation for water, shellfish (EC 2073/2005 EC B53/2004) and plant (EC 2073/2005) does...
Article
Full-text available
In this work a comparative study using Screen-Printed Electrodes (SPEs) modified by drop casting with Carbon Black, Single Walled Carbon NanotubesCOOH, Graphene Oxide, and reduced Graphene Oxide is reported. The carbon nanomaterials employed were characterized by X-ray photoelectron and Raman spectroscopy, while the modified SPEs have been morpholo...
Article
An automatable flow system for the continuous and long term monitoring of phosphate level has been developed using an amperometric detection method based on the use of a miniaturized sensor. This method is based on the monitoring of an electroactive complex obtained by the reaction between phosphate and a molybdate that is consequently reduced at t...
Article
Full-text available
Nowadays extensive volumes of pesticides are employed for agricultural and environmental practices, but they have negative effects on human health. The levels of pesticides are necessarily restricted by international regulatory agencies, thus rapid, cost-effective and in-field analysis of pesticides is an important issue. In the present work, we pr...
Article
Full-text available
A new microengine-based built-in-platform exploiting a surprising dual action with solution mixing and control of the reaction parameters, has been applied for accelerating chemical reactions (organophosphorous nerve agents hydrolysis) and electrochemical detection of non-hazardous by-product (p-nitrophenol) using printable sensor strip.
Conference Paper
Full-text available
The final aim of this work is the realization of an automatic system based on amperometric measurements able to detect the phosphate in absence of the operator, thus in remote mode control, using automatic valves leaded by a software. The SPEs (screen-printed electrodes) thank to their small size and long term stability, can be easily embedded in a...
Article
Full-text available
Here we explore a general strategy for the rational design of nucleic acid catalysts that can be allosterically activated by specific nucleic-acid binding proteins. To demonstrate this we have combined a catalytic DNAzyme sequence and the consensus sequence recognized by specific transcription factors to create a construct exhibiting two low-energy...
Article
Full-text available
We have developed a screen-printed electrochemical electrode (SPE) for paraoxon based on its inhibitory effect on the enzyme butyrylcholinesterase (BChE). The electrode was first modified by drop casting with a dispersion of carbon black nanoparticles (CBNPs) in a dimethylformamide-water mixture, and BChE was then immobilized on the surface by cros...
Chapter
A screen-printed electrode (SPE) modified with a carbon black and Au nanoparticles bilayer was developed and proposed as an amperometric sensor for ascorbic acid quantification in pharmaceutical products and for dopamine estimation in the presence of large excess of ascorbic acid. Electrochemical investigations highlight the performances of the res...
Article
Food and diet are closely linked to human health, and new emerging research fields are attempting to guarantee improvements in food quality and safety. Biosensor technology represents a cutting-edge frontier in environmental and biomedical diagnosis and is at the forefront in the agrifood sector. Smart monitoring of nutrients and fast screening of...
Article
Full-text available
Background: Archaeological bones contain only small amounts of DNA due to post-mortem DNA degradation and the changes endogenous DNA is subjected to during diagenesis. An important step before undertaking such time-consuming and costly analyses as ancient DNA investigation is to predict the presence of DNA in ancient samples. To date, the leading...
Article
Full-text available
A sensor for the simple and sensitive measurement of hydrogen peroxide has been developed which is based on screen printed electrodes (SPEs) modified with Prussian blue nanoparticles (PBNPs) deposited using piezoelectric inkjet printing. PBNP-modified SPEs were characterized using physical and electrochemical techniques to optimize the PBNP layer t...
Article
Full-text available
A novel sensor based on carbon black-gold nanoparticle nanocomposite modified screen-printed electrode (CB-AuNPs/SPE) for the detection of As(III) has been developed. The sensor was prepared modifying the SPE with CB and AuNPs by a drop casting automatable deposition. The As(III) was detected by CB-AuNPs/SPE using anodic stripping voltammetry, with...
Thesis
Full-text available
Aim: The goal of this thesis was the development of an electrochemical sensor for the determination of Peracetic acid in swimming pool water. The use of Peracetic acid for swimming pool water disinfection could be an alternative to chlorine because the PAA has several advantages compared to this latter, for example: absence of stable toxicants, low...
Article
Oxidized Single-Wall Carbon Nanohorns (o-SWCNHs) were used, for the first time, to assemble chemically modified Screen Printed Electrodes (SPEs) selective towards the electrochemical detection of Epinephrine (Ep), in the presence of Serotonine-5-HT (S-5HT), Dopamine (DA), Nor-Epineprhine (Nor-Ep), Ascorbic Acid (AA), Acetaminophen (Ac) and Uric Aci...
Article
An electrochemical sensor for palytoxin (PlTX) detection, based on a strip of eight screen-printed electrodes connected to a cost-effective and portable apparatus, is reported. Sheep erythrocytes were used to test the palytoxin detector and degree of haemolysis was evaluated by measuring release of the cytosolic lactate dehydrogenase (LDH). Percent...
Article
Full-text available
Paper based artworks are probably ones of the most difficult materials to restore, because of their complexity and fragile structure. Cleaning of paper artifacts, one of the process commonly carried out during restoration, usually involves the use of solvents (organic or not), that may cause several troubles, like swelling and dissolution of some c...
Article
Oxidized Single-Wall Carbon Nanohorns (o-SWCNHs) were used, for the first time, to assemble chemically modified Screen Printed Electrodes (SPEs) selective towards the electrochemical detection of Epinephrine (Ep), in the presence of Serotonine-5-HT (S-5HT), Dopamine (DA), Nor-Epineprhine (Nor-Ep), Ascorbic Acid (AA), Acetaminophen (Ac) and Uric Aci...