
Gil M YerushalmiAssaf Harofeh Medical Center · Department of Obstetrics and Gynecology
Gil M Yerushalmi
MD/PhD.
About
93
Publications
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Introduction
Additional affiliations
March 2005 - present
Education
March 2005 - September 2010
July 2002 - July 2003
October 1993 - July 2002
Publications
Publications (93)
Cumulus expansion and oocyte maturation are central processes in ovulation. Knowledge gained from rodent and other mammalian models has revealed some of the molecular pathways associated with these processes. However, the equivalent pathways in humans have not been thoroughly studied and remain unidentified.Compact cumulus cells (CCs) from germinal...
To evaluate the efficacy and safety of 3 months of vaginal mifepristone treatment on leiomyoma volume and related symptoms.
Prospective, open-label, two tertiary centers, phase II clinical trial.
Two tertiary medical centers in Israel.
Thirty-three enrolled women, ages 30-53 years, diagnosed with symptomatic uterine fibroids.
Patients received 10 m...
Preeclampsia, a severe unpredictable complication of pregnancy, occurs in 6% of pregnancies, usually in the second or third trimester. The specific etiology of preeclampsia remains unclear, although the pathophysiological hallmark of this condition appears to be an inadequate blood supply to the placenta. As a result of the impaired placental blood...
Ovarian follicular development and ovulation in mammals is a complex and highly regulated process. Most advances in the understanding of the ovulatory process have come from animal models. However, translational research in humans is of crucial importance for improving fertility treatment and control.
IVM/IVF procedures allow us to obtain follicula...
Met-hepatocyte growth factor/scatter factor (HGF/SF) signaling plays an important role in epithelial tissue morphogenesis, lumen formation, and tumorigenicity. We have recently demonstrated that HGF/SF also alters the metabolic activity of cells by enhancing both the glycolytic and oxidative phosphorylation pathways of energy production. Using diff...
Purpose
To investigate factors influencing long-term utilization and disposal patterns of cryopreserved semen straws in oncological patients.
Methods
This retrospective study included all men who cryopreserved semen due to cancer between October 1993 and December 2021. To assess non-used cryopreserved sperm straws, we investigated the following pa...
Although many Fertility Centers have adopted day 5 or 6 embryo transfer policy, yet, 30% of embryo transfers in the US are performed on day 3. This is mainly due to concerns related to longer embryo culture effect and higher rates of embryo transfer cancellation on day 5, with no effect on cumulative pregnancy rate. We conducted a retrospective coh...
Study question
Does the RANK-RANKL-OPG system play a role in oocyte maturation and ovulation?
Summary answer
RANKL, a new ovulatory gene expressed by cumulus and mural granulosa cells activates RANK receptors in oocytes, promoting oocyte maturation through oocyte-granulosa cell communication.
What is known already
The receptor activator of nuclea...
The aim of our study was to evaluate if the response to follicular GnRH agonist (GnRHa) trigger be used to predict intracycle ovarian response in GnRH antagonist cycles among women undergoing fertility preservation IVF. We conducted a prospective study of 146 GnRH antagonist oocyte pickup (OPU) cycles to evaluate GnRHa stimulation test (GAST). On d...
Purpose
To investigate cryopreserved testicular spermatozoa among patients with azoospermia.
Methods
In this retrospective study spanning from October 1993 to December 2021, we examined men diagnosed with azoospermia who underwent testicular spermatozoa cryopreservation. Data from medical records included utilization and disposal of sperm samples,...
This prospective study aimed to test the ability of follicular GnRH agonist challenge test (FACT) to predict suboptimal response to GnRH agonist trigger, assessed by LH levels post ovulation trigger in non-medical oocyte cryopreservation program. The study included 91 women that underwent non-medical fertility preservation. On day two to menstrual...
Background
Sperm banks face a continuously evolving gap between the increasing demand for sperm donation (SD) vs. limited available reserve. To improve donors’ recruitment and increase supply, motivations towards SD should be investigated specifically among young men who have the potential to become donors. Our aim was to evaluate factors which inc...
Background:
The wide implementation of sperm freezing presents a growing burden on sperm banks.
Objectives:
To evaluate sperm freezing and usage patterns over 30 years, according to demographic parameters of age at first cryopreservation and number of children, and indication for cryopreservation.
Material and methods:
This retrospective, popu...
M Ronen S Kaufman Alon Kedem- [...]
I Gat
Study question
What is the actual preference of sperm donors with identity disclosure vs. anonymous donors among recipient patients? Does this selection affect clinical outcomes?
Summary answer
Identity disclosure donation is important for certain sections, while almost half of the patients used anonymous donation. An Identity disclosure-only poli...
Study question
Is there an advantage for modified flare/ GnRH antagonist protocol in comparison to antagonist protocol in oocyte cryopreservation cycles?
Summary answer
Modified flare/ GnRH antagonist protocol is at least as good as antagonist protocol in oocyte cryopreservation cycles, potentially improving maturity rate in subgroup of patients....
The role of prostaglandins (PGs) in the ovulatory process is known. However, the role of the ATP binding cassette subfamily C member 4 (ABCC4), transmembrane PG carrier protein, in ovulation remains unknown. We report herein that ABCC4 expression is significantly upregulated in preovulatory human granulosa cells (GCs). We found that PGE2 efflux in...
Research question
: Are IVF treatments with extremely high peak estradiol (E2) levels and GnRH agonist trigger associated with higher complication rates?
Design
: A retrospective cohort study including patients from two large medical centers treated between 2019-2021. A study group with extremely high peak E2 levels (≥20,000 pmol/L on the day of o...
Purpose
To study the effect of SARS-CoV-2 infection on pregnancy rates in frozen embryo transfer (FET) cycles.
Methods
A retrospective cohort study including women under the age of 42 with documented SARS-CoV-2 infection up to 1 year prior to treatment, undergoing FET cycles in the first half of 2021, with transfer of embryos generated prior to th...
Purpose
To describe spermatozoa extraction rate by testicular sperm extraction (TESE) for posthumous sperm retrieval (PMSR) and examine harvest time impact on sperm motility; to compare long-term sperm usage between married vs. single deceased men.
Methods
This retrospective study included all PMSR cases in Shamir Medical Center during 2003–2021....
Study question
Does prior SARS-CoV-2 infection in women undergoing fertility treatments affect outcomes of fresh ART cycles?
Summary answer
SARS-CoV-2 infection does not affect fresh ART treatment outcomes. A possible long term negative effect on oocyte yield should be further explored.
What is known already
There is evidence that the renin–angi...
Study question
Are IVF treatments with extremely high peak estradiol levels during ovarian hyperstimulation associated with higher complication rates?
Summary answer
Extremely high estradiol levels were not associated with higher major complication rates, but patients were more likely to seek medical care following retrieval.
What is known alread...
Study question
Does the response to GnRH agonist can be used to predict ovarian function in short GnRH antagonist cycles in women undergoing oocyte cryopreservation IVF cycles?
Summary answer
Response to a single GnRH agonist dose at the beginning of stimulation during short antagonist cycle can predict the outcome of the fertility preservation cy...
Study question
What is the effect of COVID-19 infection on pregnancy rates in frozen embryo transfer (FET) cycles?
Summary answer
Past COVID-19 infection decreased pregnancy rates in FET cycles, especially in patients with recent infection.
What is known already
ACE2 and TMPRSS2 are expressed in the endometrium, potentially enabling SARS-COV-2 vi...
S Avraham Alon Kedem H Zur- [...]
E Maman
Study question
Is there an influence of mRNA COVID-19 vaccine on ovarian response and in vitro fertilization (IVF) treatment outcomes?
Summary answer
COVID-19 mRNA vaccine did not affect the ovarian response nor pregnancy rates in IVF treatment
What is known already
Studies demonstrated that infection with COVID-19 during pregnancy increased the...
Purpose
To investigate the association between endometrial compaction and pregnancy rates in unstimulated natural cycle frozen embryo transfers.
Design
A single-center prospective cohort study. Endometrial thickness by transvaginal ultrasound and blood progesterone levels on the day of ovulation and the day of embryo transfer were evaluated in pat...
Study Question
Does prior SARS-CoV-2 infection in women undergoing fertility treatments affect the outcomes of fresh ART cycles?
Summary Answer
SARS-CoV-2 infection does not affect fresh ART treatment outcomes, except for a possible long term negative effect on oocyte yield (>180 days post infection).
What Is Known Already
A single previous study...
Objective
To assess the influence of mRNA COVID-19 vaccine on ovarian response and IVF treatment outcomes.
Design
A retrospective cohort study.
Setting
A tertiary university-affiliated medical center and a private medical center.
Subjects
The study included a total of 400 patients, 200 vaccinated women and 200 age-matched unvaccinated women, und...
Based on a previous global transcriptome sequencing project, we hypothesized that Lumican (LUM) might play a role in ovulatory processes. We sought to determine LUM gene expression under various conditions in human preovulatory follicles. The in vitro expression of LUM mRNA in mural (MGCs) and cumulus (CGCs) granulosa cells was characterized using...
The superiority of day 5 blastocysts compared to day 6 blastocysts in fresh cycle transfers was previously demonstrated and attributed mainly to endometrial asynchrony. Data from frozen blastocysts transfers showed conflicting results, possibly due to heterogeneous patient population and embryo quality. The aim of this study was to compare clinical...
The role of Prostaglandins (PGs) in the ovulatory process is known. However, the role of the ATP binding cassette subfamily C member 4 (ABCC4), transmembrane PG carrier protein, in ovulation remains unknown. We report herein that ABCC4 expression is significantly upregulated in preovulatory human granulosa cells (GCs). We found that PGE2 efflux in...
Background: Ovulation is triggered by the preovulatory surge of the pituitary luteinizing hormone (LH). LH/hCG induction of reactive oxygen species (ROS) is required for successful ovulation. H2O2, one of ROS species, was shown to fully mimic the effect of LH/hCG in mice ovulation. However, the molecular process that generates H2O2 in the ovary dur...
Background:
DCN (decorin) is a proteoglycan known to be involved in regulating cell proliferation, collagen fibril organization and migration. In our global transcriptome RNA-sequencing approach to systematically identify new ovulation-associated genes, DCN was identified as one of the highly regulated genes. We therefore hypothesize that DCN may...
Ovarian follicular development and ovulation in mammals is a highly-regulated process. Most of the current knowledge of ovarian processes was obtained from the studies of non-human models. Molecular studies on human ovarian processes suffer from lack of material and appropriate research tools. Mural granulosa cells (MGCs) culture is a major tool fo...
Background
The cumulus expansion process is one of the LH mediated ovulatory processes. Hyaluronan synthase 2 (HAS2) regulates the synthesis of hyaluronic acid, the main component of the cumulus expansion process. Recently, the lncRNA HAS2 antisense RNA 1 (HAS2-AS1) was identified in our global transcriptome RNA-sequencing of novel ovulation associ...
Ovarian follicular development and ovulation are complex and tightly regulated processes that involve regulation by microRNAs (miRNAs). We previously identified differentially expressed mRNAs between human cumulus granulosa cells (CGCs) from immature early antral follicles (germinal vesicle - GV) and mature preovulatory follicles (metaphase II - M2...
PurposeFew clinical options for fertility preservation are available to females with cancer, and data about clinical outcomes is limited. Potential supplementary approaches to fertility preservation include retrieval of immature oocytes followed by in vitro maturation (IVM) and storage. The aim of this study was to evaluate post-thawing outcomes of...
PurposePartial thawing of a vial of cryopreserved sperm (shaving) is sometimes applied as a measure to preserve sperm for further use, particularly in cases of very restricted sperm quantity. However, mechanical violence may disrupt the sperm-wall and lead to impaired in-vitro fertilization (IVF) outcomes. Material and methodsIn a retrospective cas...
Prostaglandins (PGs) play an important role in the ovulatory process. However, the role of the PG transporter (PGT) in this context remains unknown. We report that the expression of PGT, a transmembrane PG carrier protein, is markedly up-regulated in preovulatory human granulosa cells (GCs). Treatment with human chorionic gonadotropin (hCG), an ovu...
STUDY QUESTION
Does Bisphenol A (BPA) impair steroid hormone production in human luteinized granulosa cells in vitro?
SUMMARY ANSWER
At supra-physiological concentrations, BPA alters progesterone and estradiol synthesis in vitro and significantly reduces the mRNA and protein expression levels of three genes encoding steroidogenesis enzymes.
WHAT...
Cumulus expansion and oocyte maturation are central processes in ovulation. Knowledge gained from rodent and other mam-malian models has revealed some of the molecular pathways associated with these processes. However, the equivalent pathways in humans have not been thoroughly studied and remain unidentified. Compact cumulus cells (CCs) from germin...
Prostaglandins (PGs) play an important role in the ovulatory process. However, the role of the PG transporter (PGT) in this context remains unknown. We report that the expression of PGT, a transmembrane PG carrier protein, is markedly up-regulated in preovulatory human granulosa cells (GCs). Treatment with human chorionic gonadotro-pin (hCG), an ov...
The aim of this study was to evaluate the safety and efficacy of combined ovarian tissue cryopreservation and oocyte aspiration just before ovarian tissue cryobanking. A retrospective cohort study of fertility preservation patients treated in 2007-2013 in one tertiary centre was performed. A total of 255 cancer patients were admitted for fertility...
Background
Serum AMH is declining with age and is highly associated with ovarian follicular reserve and disordered folliculogenesis. However, the precise role of AMH in the process of human follicular aging has still to be determined.AimThis study investigates AMH level in the follicular fluid (FF) and mRNA expression pattern in cumulus and mural g...
Objective
To determine the prevalence of placental complications in patients conceived through donor versus autologous oocytes.
Study Design
A retrospective cohort study including two groups of patients who conceived through in vitro fertilization using: 1) donor oocyte (n=139) and 2) autologous oocyte (n=126). Only singleton gestations were inclu...
Objective
To examine whether patients with poor ovarian response (POR) during conventional IVF/intracytoplasmic sperm injection (ICSI) treatment cycle may benefit from a modified natural cycle (MNC)-IVF.
Design
Cohort historic study.
Setting
Tertiary, university-affiliated medical center.
Patient(s)
One hundred eleven patients with POR, defined...
Abstract Poliovirus receptor (PVR), regulator of G-protein signaling-11 (RGS11), and erythrocyte protein band-4.1-like 3 (EPB41L3) have been proposed to function in follicular maturation in mouse models. We have examined their expression in human mural (mGCs) and cumulus granulosa cells (CCs). Expression of PVR and RGS11 in mGCs decreased in medium...
Controlled ovarian hyperstimulation (COH) which combining GnRH antagonist co-treatment and GnRH agonist trigger with an additional 1500 IU hCG luteal rescue on day of oocytes retrieval, has become a common tool aiming to reduce severe ovarian hyperstimulation syndrome (OHSS). In the present, proof of concept study, we evaluate whether by deferring...
Introduction:
There are two most popular protocols for Frozen Embryo Transfer: the natural and the E2&P4 replacement cycles. There is still a controversy whether one is superior over the other.
Purpose:
To compare the outcome in patient groups undergoing FET following these protocols.
Methods:
About 1235 FET cycles were retrospectively analyze...
To investigate the mRNA expression of genes related to steroidogenesis and OHSS in granulosa cells (GCs) of patients triggered with GnRH agonist compared to patients triggered with hCG.
Mural GCs were obtained at the time of oocyte retrieval and gene expression was analyzed using quantitative real time RT-PCR.
Single center, case control study.
24...
One of the suggest strategy for patients with repeated implantation failure (RIF) is zygote intrafallopian transfer (ZIFT). However, no data exist regarding to the issue of when and under which circumstances should ZIFT be offered to patients with RIF? We therefore aimed to examine whether repeated implantation failure (RIF) patients characteristic...
Objective:
To compare the outcome of vitrification versus slow freezing cryopreservation for cleavage stage day 2-3 embryos.
Design:
A retrospective observational study.
Setting:
All thawed embryos assisted reproduction cycles between January 2010 and December 2012 at a single IVF laboratory of a Tertiary Medical Center.
Patients:
Five hundr...
The ideal test for ovarian reserve should permit the identification of women who have no real chance of pregnancy with IVF treatments consequent upon an extremely reduced ovarian reserve. The aim of the current study was to evaluate pregnancy rates in patients with low AMH levels (0.2-1 ng/ml) and extremely low AMH levels (<0.2 ng/ml) and to determ...
Abstract Progesterone, the main steroid synthesized by the corpus luteum (CL), prepares the uterus for implantation, maintains the CL survival, and induces progesterone auto-secretion. However, the molecular mechanisms involving the progesterone auto-secretion pathways at the luteal phase are not fully understood, especially in humans. We aim to st...
Purpose:
To prospectively study the AMH expression and secretion pattern in mural granulosa cells (GCs) and follicular fluid (FF) from small follicles and medium follicles that were collected from normo-ovulatory (NO) and polycystic ovary syndrome (PCOS) patients undergoing in vitro maturation (IVM) treatments.
Methods:
FF AMH levels and mRNA ex...
Purpose:
To assesse circulating levels of Anti-Müllerian hormone (AMH) as a predictor of oocyte number and their potential to mature in vitro in both normo-ovulatory (NO) women and in women with Polycystic Ovary Syndrome (PCOS) undergoing in vitro maturation (IVM) treatments.
Methods:
We prospectively studied NO women and women diagnosed with PC...
We present two patients who delivered vaginally following a prior cesarean section in whom uterine rupture was detected a few minutes after delivery. Both deliveries occurred within 12 h. The sonographic findings were different in each case and consistent with the clinical and operative findings. Ultrasound may assist in the diagnosis of uterine ru...
The objective of this study was to evaluate the prognosis of patients with a first treatment cycle that was defined as a poor ovarian response cycle according to the new ESHRE consensus criteria. The first documented cycle of poor response for a patient and all the cycles that followed were retrospectively analysed. Factors that were associated wit...
Aim:
To evaluate the effect of local injury to the endometrium during spontaneous menstrual cycles before in vitro fertilization (IVF) treatment on implantation and pregnancy rates in women with recurrent implantation failure (RIF).
Methods:
In a prospective randomized controlled trial (RCT), a total of 36 patients, with RIF undergoing IVF, were...
Early pregnancy loss is common among women treated with assisted reproduction treatment, but whether it is a prognostic factor for success in subsequent IVF cycles is not well established. The aim of this study was to determine whether a biochemical pregnancy (BP) or spontaneous abortion (SA) affects the pregnancy rates in the following cycle. A re...
Selected genes induced by (A) hypoxia and (B) p53.
(PDF)
Analysis of the study population: Significant results (p<0.05) are highlighted by a grey background. Average (Avg), standard deviation (SD), interquartile range (IQR), hypoxia (H), normal (N).
(JPG)
Characteristics of the study cohort: H, Hypoxia; N, Normal; NA, Not available; G, Number of pregnancies; P, Previous pregnancies; HTN, Hypertension; *, Smoker; **, Twins; ***, Dollberg 2005.
(PDF)
Delta Ct and Delta-Delta Ct calculations for the study cohort.
(PDF)
To elucidate the LH receptor (LHR) expression patterns in human granulosa cells (GCs) from antral to preovulatory stages, and to investigate a correlation to oocyte function.
Luteinized preovulatory GCs were obtained from preovulatory follicles aspirated during IVF (≥ 17 mm). The GCs from small- (<10 mm) and medium-sized (10-15 mm) follicles were o...