Gianfranco Santovito

Gianfranco Santovito
University of Padova | UNIPD · Department of Biology

PhD

About

152
Publications
20,103
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
2,206
Citations
Citations since 2017
92 Research Items
1673 Citations
20172018201920202021202220230100200300400500
20172018201920202021202220230100200300400500
20172018201920202021202220230100200300400500
20172018201920202021202220230100200300400500
Additional affiliations
October 2002 - present
University of Padova
Position
  • General Physiology, Animal Physiology and Biology Didactics
October 1996 - present
University of Padova
Position
  • Physiological responses anaginst metal accumulation and reactive oxygen species generation
October 1993 - September 1996
University of Padova
Position
  • Copper/zinc superoxide dismutase in yeasts and bacteria
Education
October 1993 - September 1996
University of Padova
Field of study
  • Biology
October 1985 - July 1990
University of Padova
Field of study
  • Biology

Publications

Publications (152)
Preprint
Full-text available
Antarctica is the continent with the lowest local human impact, yet it is still vulnerable to contaminants coming from external sources. Emerging pollutants, like PFAS, pose an increasing threat to this environment and therefore require more in-depth investigations to understand their environmental fate and biological impacts. The present study, pa...
Article
Full-text available
The Antarctic continent is one of the most pristine environments on planet Earth, yet one of the most fragile and susceptible to the effects of the ongoing climate change. The overwhelming majority of the components of Antarctic marine trophic chain are stenotherm organisms, highly adapted to the extreme, but extremely stable, freezing temperatures...
Article
Full-text available
The Antarctic endemic fish genus Trematomus (Trematominae, Notothenioidei) includes 15 species very diverse in morphology, lifestyle and feeding ecology. Co-occurring on the continental shelf, they occupy different habitats and a wide range of ecological niches as the result of adaptive radiation during their evolutionary history. Ecomorphological...
Article
Full-text available
The Frank-Starling response is an intrinsic heart property that is particularly evident in the fish heart because piscine cardiomyocytes are extremely sensitive to stretch. Several mechanisms and compounds influence the Frank-Starling response, including the free radical nitric oxide produced by nitric oxide synthases in the vascular endothelium an...
Article
Full-text available
Metallothioneins (MTs) are low-molecular weight sulfur-rich proteins, widely distributed in nature. They play a homeostatic role in the control and detoxification of metal ions. Previous studies indicated that MTs also have the capacity to scavenge reactive oxygen species. This study aimed to investigate the evolution of the protein in the notothen...
Article
Full-text available
Metal bioaccumulation and metallothionein (MT) expression were investigated in the gills and liver of the red-blooded Antarctic teleost Trematomus hansoni to evaluate the possibility for this species to face, with adequate physiological responses, an increase of copper and cadmium concentrations in its tissues. Specimens of this Antarctic fish were...
Article
Full-text available
This work aimed to evaluate the effects of zinc (Zn) relating to cadmium (Cd)-induced toxicity and the role played by MTF-1. This transcription factor regulates the expression of genes encoding metallothioneins (MTs), some Zn transporters and the heavy chain of γ-glutamylcysteine synthetase. For this reason, two cell lines of mouse fibroblasts were...
Conference Paper
Mediterranean mussel (M. galloprovincialis) is the most common bivalve species in the Mediterranean sea, able to survive across broad ranges of temperature and salinity. Albania is the 4th largest European producer of M. galloprovincialis, after Italy, Greece and France and there is an increasing trend. However, Mediterranean mussels is vehicles in...
Preprint
Full-text available
Metal bioaccumulation and metallothionein (MT) expression were investigated in gills and liver of the red-blooded Antarctic teleost Trematomus hansoni with the aim to evaluate the possibility for this species to face, with adequate physiological responses, an increase of copper or cadmium concentrations in the environment. Specimens of this Antarct...
Preprint
Metal bioaccumulation and metallothionein (MT) expression were investigated in gills and liver of the red-blooded Antarctic teleost Trematomus hansoni with the aim to evaluate the possibility for this species to face, with adequate physiological responses, an increase of copper or cadmium concentrations in the environment. Specimens of this Antarct...
Article
Full-text available
In recent decades, the interest in PFAS has grown exponentially around the world, due to the toxic effects induced by these chemical compounds in humans, as well as in other animals and plants. However, current knowledge related to the antistress responses that organisms can express when exposed to these substances is still insufficient and, theref...
Preprint
In recent decades, the interest in PFAS has grown exponentially around the world, due to the toxic effects induced by these chemical compounds in humans, as well as in other animals and in plants. However, current knowledge related to the antistress responses that organisms can express when exposed to these substances is still insufficient and ther...
Article
Full-text available
Per- and polyfluoroalkyl substances (PFASs) have multiple emission sources, from industrial to domestic, and their high persistence and mobility help them to spread in all the networks of watercourses. Diffuse pollution of these compounds can be potentially mitigated by the application of green infrastructures, which are a pillar of the EU Green De...
Article
Full-text available
Insufficient supply of cardiac grafts represents a severe obstacle in heart transplantation. Donation after circulatory death (DCD), in addition to conventional donation after brain death, is one promising option to overcome the organ shortage. However, DCD organs undergo an inevitably longer period of unprotected warm ischemia between circulatory...
Article
Full-text available
Typical 2-Cys peroxiredoxins (2-Cys Prdxs) are proteins with antioxidant properties belonging to the thioredoxin peroxidase family. With their peroxidase activity, they contribute to the homeostatic control of reactive oxygen species (ROS) and, therefore, participate in various physiological functions, such as cell proliferation, differentiation, a...
Article
Full-text available
Antarctica represents a unique natural laboratory for ecotoxicological studies as it is characterized by low internal pollutants emissions but high external contamination levels. Indeed, warm temperatures promote pollutant evaporation (low latitudes), while cool temperatures (high latitudes) promote its deposition from the atmosphere on land/water....
Article
The stenothermal Antarctic fish that live in the coastal waters of the Terra Nova Bay (Ross Sea) are rarely exposed to temperatures above zero during the year. We tested whether a slight temperature rise of 1.5 °C affects a sensitive biomarker such as erythrocytes morphology in sections of blood pellets of a small demersal notothen. The erythrocyte...
Preprint
Full-text available
Typical 2-Cys peroxiredoxins (2-Cys Prdxs) are proteins with antioxidant properties belonging to the thioredoxin peroxidase family. With their peroxidase activity, they contribute to the homeostatic control of reactive oxygen species (ROS) and, therefore, participate in various physiological functions such as cell proliferation, differentiation, an...
Article
Full-text available
There is growing concern for the wide use of perfluorooctanoic acid (PFOA) because of its toxic effects on the environment and on human health. A new compound-the so called C6O4 (perfluoro ([5-methoxy-1,3-dioxolan-4-yl]oxy) acetic acid)-was recently introduced as one of the alternative to traditional PFOA, however this was done without any scientif...
Article
Full-text available
It is well known that the excessive production of reactive oxygen species (ROS) can lead to the peroxidation of membrane lipids, glycation/oxidation/nitration of proteins, inactivation of enzymes, DNA mutation and damage, and other alterations in the subcellular components [...]
Article
Full-text available
Far from being devoid of life, Antarctic waters are home to Cryonotothenioidea, which represent one of the fascinating cases of evolutionary adaptation to extreme environmental conditions in vertebrates. Thanks to a series of unique morphological and physiological peculiarities, which include the paradigmatic case of loss of hemoglobin in the famil...
Preprint
Full-text available
We here report the de novo transcriptome assembly and functional annotation of Eusirus cf. giganteus clade g3, providing the first database of expressed sequences from this giant Antarctic amphipod. RNA-sequencing, carried out on the whole-body of a single juvenile individual likely undergoing molting, revealed the dominant expression of hemocyanin...
Article
The exposure to metals is known to generate oxidative stress in living organisms, which can result in the induction of protective antioxidant defences, both enzymatic and non-enzymatic. This work aims to obtain new data on the existing links among several non-enzymatic components of the antioxidant system, that are physiologically related to both m...
Article
Stress granules are non-membranous cytoplasmic foci, composed of non-translating messenger ribonucleoproteins, translational initiation factors and other additional proteins. They represent a primary mechanism to rapidly modulate gene expression when cells are subjected to adverse environmental conditions. Very few works have been devoted to study...
Article
h i g h l i g h t s Risks related to metal contamination in Antarctica. Effects of waterborne Cd and Cu on fish oogenesis. Oocyte degeneration and changes in glycan composition. Cd downregulation of alpha and beta estradiol receptors. Cd and Cu reduce fecundity in T. bernacchii by impairing stock recruitment. a b s t r a c t Antarctica has long bee...
Conference Paper
Full-text available
In the last decade, Science Education has played a crucial role in the training of young students, supporting them in understanding the world around us, making them active citizens, responsible and capable of innovation in a knowledge-based society (Recommendation of the European Parliament and of the Council, 18 December 2006). The purpose of the...
Conference Paper
Full-text available
With a view to the active learning, that makes the students protagonist, responsible and aware of the learning process, the present study presents an experimental work designed to demonstrate the usefulness and the validity of the scientific method as the basic element of the teaching of Life Sciences. Scientific training adopts an inductive approa...
Conference Paper
Full-text available
In the last decade, Science Education has played a crucial role in the training of young students, supporting them in understanding the world around us, making them active citizens, responsible and capable of innovation in a knowledge-based society (Recommendation of the European Parliament and of the Council, 18 December 2006). The purpose of the...
Conference Paper
Full-text available
In 2005 the United Nations promoted the "decade of education for sustainable development" (2005-2014) and the UNESCO Italian Committee instituted the annual "week of education for sustainable development" to raise awareness about the necessity of a fairer and more respectful future for both human beings and environment. Nevertheless, the Italian Gu...
Conference Paper
Full-text available
This study falls in the field research of Life Science didactics. According to the main Italian (Italian Guidelines for the Curriculum in the Primary School-2012 Edition) and European law documents (Recommendation of the European Parliament and of the Council, 18 December 2006), and to the scientific research as regards this subject, it seems to be...
Conference Paper
Full-text available
With a view to the active learning, that makes the students protagonist, responsible and aware of the learning process, the present study presents an experimental work designed to demonstrate the usefulness and the validity of the scientific method as the basic element of the teaching of Life Sciences. Scientific training adopts an inductive approa...
Conference Paper
Full-text available
The present research compares the results obtained by using two different methods of instruction in teaching Darwin's evolution theory: laboratory activities and traditional lessons based on textbooks. We built up a teaching path on evolution using laboratory activities, in order to highlight how the main concepts of this theory (selection, adaptat...
Conference Paper
Full-text available
With a view to the active learning, that makes the students protagonists, responsible and aware of the learning process, the present study presents an experimental work designed to demonstrate the usefulness and the validity of the scientific method as the basic element of the teaching of Life Sciences. The experimental research was implemented in...
Article
Full-text available
Due to their unique properties, perfluorinated substances (PFAS) are widely used in multiple industrial and commercial applications, but they are toxic for animals, humans included. This review presents some available data on the PFAS environmental distribution in the world, and in particular in Europe and in the Veneto region of Italy, where it ha...
Preprint
Full-text available
Due to their unique properties, perfluorinated substances (PFAS) are widely used in multiple industrial and commercial applications, but they are toxic for animals, humans included. This review presents some available data on the PFAS environmental distribution in the world, and in particular in Europe and in Veneto region of Italy, where it has be...
Article
Full-text available
Glutathione peroxidases (GPxs) form a broad family of antioxidant proteins essential for maintaining redox homeostasis in eukaryotic cells. In this study, we used an integrative approach that combines bioinformatics, molecular biology, and biochemistry to investigate the role of GPxs in reactive oxygen species detoxification in the unicellular euka...
Preprint
Full-text available
Glutathione peroxidases (GPxs) form a broad family of antioxidant proteins essential for maintaining redox homeostasis in eukaryotic cells. In this study, we used an integrative approach that combines bioinformatics, molecular biology, and biochemistry to investigate the role of GPxs in reactive oxygen species detoxification in the unicellular euka...
Article
Full-text available
With a view to the active learning, that makes the students protagonist, responsible and aware of the learning process, the present study presents an experimental work designed to demonstrate the usefulness and the validity of the scientific method as the basic element of the teaching of Life Sciences. Scientific training adopts an inductive approa...
Article
Full-text available
In the last decade, Science Education has played a crucial role in the training of young students, supporting them in understanding the world around us, making them active citizens, responsible and capable of innovation in a knowledge-based society (Recommendation of the European Parliament and of the Council, 18 December 2006). The purpose of the...
Preprint
Full-text available
Exposure to metals is known to generate oxidative stress in living organisms, which are able to respond with the induction of antioxidant defenses, both enzymatic and non-enzymatic. The aim of this work is to study the correlation among several non-enzymatic component of the antioxidant system, that are physiologically related to both metal sequest...
Article
Full-text available
In the present study, we describe the purification and molecular characterization of Cu,Zn superoxide dismutase (SOD) from Chionodraco hamatus, an Antarctic teleost widely distributed in many areas of the Ross Sea that plays a pivotal role in the Antarctic food chain. The primary sequence was obtained using biochemical and molecular biology approac...
Article
Full-text available
Peroxiredoxins are antioxidant enzymes that use redox active Cys residues to reduce H2O2 and various organic hydroperoxides to less reactive products, and thereby protect cells against oxidative stress. In yeasts and mammals, the Prx1 proteins are sensitive to hyperoxidation and consequent loss of their peroxidase activity whereas in most bacteria...
Article
Full-text available
Antarctic fish belonging to Notothenioidei represent an extraordinary example of radiation in the cold. In addition to the absence of hemoglobin, icefish show a number of other striking peculiarities including large-diameter blood vessels, high vascular densities, mitochondria-rich muscle cells, and unusual mitochondrial architecture. In order to i...