Ghorbanali Nematzadeh

Sari Agricultural Sciences and Natural Resources University | SANRU

Plants have acquired sets of highly regulated and complex signaling pathways to respond to unfavorable environmental conditions during evolution. Calcium signaling, as a vital mechanism, enables plants to respond to external stimuli, including abiotic and biotic stresses, and coordinate the basic processes of growth and development. In the present...

Plants have acquired sets of highly regulated and complex signaling pathways to respond to unfavorable environmental conditions during evolution. Calcium signaling, as a vital mechanism , enables plants to respond to external stimuli, including abiotic and biotic stresses, and coordinate the basic processes of the growth and development. In the pre...

The use of wild plant species or their halophytic relatives has been considered in plant breeding programs to improve salt and drought tolerance in crop plants. Aeluropus littoralis serves as halophyte model for identification and isolation of novel stress adaptation genes. A. littoralis, a perennial monocot grass, grows in damp or arid areas, ofte...

Objective: In contrast to glycophytes, halophyte plants have evolved unique morphological and physiological mechanisms to deal with abiotic stress. This study presents the physiological responses of Aeluropus littoralis, a halophyte grass, to salt stress and recovery conditions on the molecular level. Results: Elemental analysis showed that Na+...

The emergence of new types of contaminants, such as pharmaceutical drugs, endocrine disrupting compounds, pesticides, herbicides, and many others, is causing serious concern because of their potential to enter the environment and cause hazardous effects to the environment and/or human health. The removal of emerging contaminants (ECs) from the envi...

Introduction: Antibacterial, antifungal and antioxidant activity of methanolic extracts of Colocynth (Citrullus colocynthis) seeds, Camelthorn (Alhagi maurorum) fruit and Chinaberry (Melia azedarach) leaves on eight bacteria and three fungi, which usually cause damage to agricultural products examined in this research. Material and Methods: Antimic...

The presence of trace contaminants, such as pharmaceuticals and personal care products (PPCPs), in various environmental media (soil, sediments, groundwater, and surface water), is a growing concern. This is a serious issue due to a lack of understanding about the potential consequences of their occurrence, fate, and eco-toxicological impacts. Nume...

As much as human beings become aware of the side effects of chemical pesticides, the demand for natural alternatives increases. In this study, antibacterial effects of different concentrations of methanolic extract of henna leaves (0.50, 0.25, 0.12, 0.06, 0.03 and 0.01 mg/mL DMSO) on three gram-positive bacteria (Bacillus subtilis, Staphylococcus a...

Following the ban on the use of in-feed antimicrobials, necrotic enteritis (NE) NE is the most important clostridial disease. Vaccination has been considered as a possible approach to prevent NE. Our previous study showed that a chimeric protein product consisting of antigenic epitopes of NetB, Alpha-toxin and Zinc metallopeptidase (Zmp) triggered...

Salinity is one of the most important growth-limiting factors for most crops in arid and semi-arid regions; however, the use of plant growth-promoting rhizobacteria isolated from saline soils could reduce the effects of saline stress in crops. This study aimed to evaluate the efficiency of plant growth-promoting rhizobacteria (PGPRs), isolated from...

Background: The production of reactive oxygen species (ROS) is a common feature of various plant cells in dealing with abiotic stresses. Plants have developed an effective enzymatic and non-enzymatic antioxidant defense systems to scavenge ROS and cope with oxidative stress. Halophyte antioxidant defense mechanisms represent one of the best example...

Background: The use of wild plant species or their halophytic relatives has been considered in plant breeding programs to improve salt and drought tolerance in crop plants. Aeluropus littoralis serves as halophyte model for identification and isolation of novel stress adaptation genes. This species is described as perennial monocot grass. A. littor...

Background: The use of wild plant species or their halophytic relatives has been considered in plant breeding programs to improve salt and drought tolerance in crop plants. Aeluropus littoralis serves as halophyte model for identification and isolation of novel stress adaptation genes. This species is described as perennial monocot grass. A. littor...

Five cyanobacterial strains with Nostoc-like morphology from different localities of the Mazandaran province of Iran were characterized using a polyphasic approach. Three strains clustered within the Aliinostoc clade whereas one each of the remaining two strains clustered within the genera Desmonostoc and Desikacharya. The phylogenetic positioning...

Azolla is a wealthy source of β-carotene. The purpose of this study was to isolate, purify and identify the β-carotene in Azolla Pinnata R. Br. plant. In this order, dry and fresh of A. Pinnata R. Br. were used and their β-carotene was extracted using the Acetone/Hexane solvent system. For the purification of β-carotene extracted, the column chroma...

This study was aimed to evaluate the efficiency of plant growth-promoting rhizobacteria (PGPRs) isolated from the rhizosphere of halophile plants on the growth, Na + /K + balance, ethylene emission, and gene expression of wheat seedlings (Triticum aestivum L.) grown under saline conditions (100 mM NaCl) for 14 days. A total of 118 isolates obtained...

Necrotic enteritis (NE) is a multifactorial disease in broiler that is caused by colonization of Clostridium perfringens in their gastrointestinal tract. Recently several immunogenic proteins from virulent C. perfringens have been considered as vaccines to provide protection against NE. In this study, a novel trivalent fusion protein including immu...

Effluent from dairy industry has a high amount of nutrients such as nitrate and phosphate. In this work nitrate and phosphate removal from treated dairy wastewater in the presence of organic load was investigated. For this purpose, 400 ml of synthetic wastewater was inoculated with 2 ml of seed culture of microalgae Chlorella salina. During the gro...

Background: Salinity as a most significant environmental challenges affects the growth and productivity of plants worldwide. In this study, the ionic and iso-osmotic effects of salt stress were investigated in Aeluropus littoralis L., a halophyte grass species from Poaceae family, by cDNA-amplified fragment length polymorphism (cDNA-AFLP) techniqu...

Avian necrotic enteritis (NE) is a multi-virulence disease caused by the bacterium Clostridium perfringens. Several toxins of this bacteria are components of different candidate vaccines, and have been considered as important factors in pathogenesis of NE. A fusion subunit protein composed of immunodominant segments of NetB, Alpha-toxin and metallo...

This experiment was carried out in both pot and field conditions to assess the effects of three native potassium solubilizing bacteria (KSB: including Pantoea agglomerans, Rahnella aquatilis and Pseudomonas orientalis which were isolated from paddy fields) on grain yield (GY), dry matter remobilization (DMR) and translocation (DMT), dry matter tran...

Abstract The current research was performed to investigate the effects of three potassium-solubilizing bacteria (KSB) strains (Pantoea agglomerans, Rahnella aquatilis, and Pseudomonas orientalis) on leaf photosynthetic characteristics in rice (Oryza sativa L. cv. Pajohesh). A pot and a field experiment were conducted in a paddy field. The results i...

The current study describes a simple method to determine four organophosphate insecticides (i.e., ethion, phosalone, diazinon, chlorpyrifos)and an isothiazolidine acaricide (i.e., hexyhiazox), as examples of pesticides in environmental water samples by stir bar sorptive extraction (SBSE). For this purpose, a self-magnetic nanocomposite monolithic (...

In this study, microalgae growth in the synthetic wastewater and their ability to remove nutrients under different light levels was investigated. For this purpose, a comparative study was conducted on freshwater microalgae Scenedesmus sp. to evaluate their performance to remove nitrate and phosphate from both slaughterhouse and dairy synthetic trea...

Background Salinity expansion in arable land is a threat to crop plants. Rice is the staple food crop across several countries worldwide; however, its salt sensitive nature severely affects its growth under excessive salinity. FL478 is a salt tolerant indica recombinant inbred line, which can be a good source of salt tolerance at the seedling stage...

It is commonly accepted that bacteria actively interact with plant host and have beneficial effects on growth and adaptation and grant tolerance to various biotic and abiotic stresses. However, the mechanisms of plant growth promoting bacteria to communicate and adapt to the plant environment are not well characterized. Among the examined bacteria...

In this work, a rapid, simple, and environmentally friendly method has been proposed for direct supramolecular microextraction of four organophosphate insecticides (ethion, phosalone, diazinon, chlorpyrifos) and an isothiazolidine acaricide (hexythiazox) in agricultural product samples prior to their determination by high-performance liquid chromat...

Salinity is one of the most important limitation factors in development of agricultural products. Cotton has a relative tolerance to salinity; however, salinity reduces its growth during germination and seedling stages. Split-factorial design of time based on randomized complete block design with 3 replications was used. The real-time PCR results f...

Abstract The current study was carried out to investigate the effects of three KSB strains of Pantoea agglomerans, Rahnella aquatilis and Pseudomonas orientalis on nitrogen (N), phosphorous (P) and potassium (K) uptake, nutrient use efficiency parameters and nutrients remobilization in rice (Oryza sativa L. Cv. Pajohesh). A pot and a field experime...

Reuse of polyethylene terephthalate (PET) bottles for storage of water and liquid food is so common in some countries of the world. However, it can result in the migration of plastic components or additives into the stored liquids and threatening of human health. In this respect, the present study developed a method for determination of endocrine d...

The correct isolation of nucleic acid from various cells is an important preliminary step before many biochemical and diagnostic processes such as cloning, sequencing, replication, hybridization, and complementary DNA (cDNA) synthesis. In this study, the coated magnetic nanoparticles (MNFs) with Tween 20 and oleic acid because of paramagnetic and b...

Salinity plays a major role in soil degradation, which reduces agricultural productivity. Cotton is one of the oil-crop plants and an important economic product. Which is relative tolerance to salt tolerance. In this study, the effect of salinity on the antioxidant enzyme activity of two cultivars of cotton, sepid (tolerant) and thermos 14 (sensiti...

The present study was intended to isolate potassium-solubilizing bacteria (KSB) from paddy rhizosphere soil. The isolates were obtained from 40 rice paddy fields across Mazandaran province in northern Iran and screened for their K-solubilizing ability on modified Aleksandrov agar medium. The three selected isolates which showed the best solubilisat...

Premise of the Study High‐yield pure chloroplast DNA (cpDNA) is necessary for whole genome sequencing. Chloroplast extraction with traditional high‐salt methods causes damage to nuclei and destroys the integrity of organelles, which leads to high genomic contamination from the nucleus and mitochondria. To overcome this issue, we modified a traditio...

Today due to mal-usage of natural resources and using improper technologies for agricultural products, a wide areas of the Iran have been encountered with salinity problems in water and soil. Therefore, it is possible to increase salt tolerance in rice via mutation induction. Present study has been done at experimental field of Genetic and Biotechn...

Abstract: Salinity is one of the tremendous problem worldwide. Although plants illustrated different sensitivity when encountered with salt stress, high level of salinity will result in water deficit and increasing toxic ions in most of plant species and together with the other sorts of stress (biotic or abiotic) plays restriction role in agricultu...

Background: Biotechnologic methods are common for secondary metabolites production from the plants and other sources in pharmaceutical sciences. Hairy root cell lines as the biotechnologic method have been used for in vitro production of major plant metabolites. Objective: In this study, hairy roots of Hypericum Perforatum have been prepared using...

Two strains of true branched heterocytous cyanobacteria, SA33 and SA16, isolated from dried paddy fields of Mazandaran, Iran, were studied using morphological, ecological, and molecular tools. Morphological characterization of the strains indicated them to be commonly showing profuse T-type branching, large irregularly shaped cells of main filament...

Evaluation of Salinity Tolerance allotetraploid Cotton (Gossypium sp.) Genotypes Using Multivariate Statistical Methods and Stress Tolerance Indices at Germination Stage Nafiseh Taghizadeh1, Gholam Ali Ranjbar1, *, Ghorban Ali Nematzadeh2, Mohammad Reza Ramazani Moghaddam3 1 Ph.D. Student Department of Plant Breeding and Biotechnology, Sari Agricul...

One method extensively used for the quantification of gene expression changes and transcript abundances is reverse-transcription quantitative real-time PCR (RT-qPCR). It provides accurate, sensitive, reliable, and reproducible results. Several factors can affect the sensitivity and specificity of RT-qPCR. Residual genomic DNA (gDNA) contaminating R...

Soil salinity is an important limitation for use of cultivated land. Crop products are influenced by salt stress greatly. Salinity has a decreasing effect on various germination and vegetative characteristics of crops. Cotton is most important natural and industrial plant in the world, used in fiber and oil industries. Although cotton reported as a...

This research investigates the chemical componds of Astragalus alopecurus Pall. fruits essential oil, and evaluates the antimicrobial effects of fruits on different plant pathogens. The essential oil of Astragalus alopecurus Pall. fruits was analyzed by GC/MS. Antimicrobial effect was examined on 14 microorganisms, containing eight bacteria and six...

Aeluropus littoralis, a member of the Poaceae family, is a monocot halophyte grass. A. littoralis is a valuable genetic resource for understanding the molecular mechanisms of stress-responsive genes. Identification of the stress response involved genes might help to improve tolerance to abiotic stresses in economically important crops. Firstly, nuc...

Abstract: Cotton is one of the oil-fluffy plants and known as an important economic product. Genetic, developmental, and molecular studies of cotton requires high-quality RNA from various tissues. Due to the richness of poly-phenol and polysaccharides, Trizol and other commercial kits are not suitable for isolating RNA from cotton tissues. Existing...

Glycine max (L.) Merrill (soybean) is a major leguminous crop, cultivated globally as well as in Iran. This study examines the chemical composition of soybean essential oil, and evaluates the antioxidant and antimicrobial activities of seeds on various plant pathogens that commonly cause irreparable damages to agricultural crops. The essential oil...

Background The use of stably expressed genes as normalizers has crucial role in accurate and reliable expression analysis estimated by quantitative real-time polymerase chain reaction (qPCR). Recent studies have shown that, the expression levels of common housekeeping genes are varying in different tissues and experimental conditions. The genomic D...

p>Plant roots can be manipulated by Agrobacterium rhizogenes to stimulate the production of heterologous proteins for pharmaceutical applications as green cell-factories. During the present study, four bacterial strains (A4, ATCC15834, ATCC11325 and A13) in combination with three co-cultivation media (MS, B5, LS) were examined to establish an effic...

Aeluropus littoralis is a monocot halophyte grass and provide valuable genetic resources for understanding the molecular mechanisms of stress-responsive genes, and improving tolerance to abiotic stresses in economically important crops. In an attempt to identify salt stressed responsive genes, 154 isolated expressed sequence tags (EST) from A. litt...

Abstract Salt is one of important restriction factors for development of agriculture crops. Although cotton is known as a salt tolerance crops, there are different levels of tolerance in various cultivars. In present work we investigated on two cultivars of cotton (thermus 14 & bakhtegan) based on completely randomized design with three replication...