Ghiles Grine

Ghiles Grine
Aix-Marseille Université | AMU · Faculté d'Odontologie

PhD
Assistant associé des Universités

About

38
Publications
6,003
Reads
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264
Citations
Citations since 2016
38 Research Items
265 Citations
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2016201720182019202020212022020406080
2016201720182019202020212022020406080
Introduction
Ghiles Grine currently works as assistant at the Faculté de Médecine, Aix-Marseille Université. Ghiles does research in bacteriology , Microbiology and Molecular Biology mastering perfectly anaerobic culture. Currently, Ghiles works on methanogenic archaea in human's microbiota.
Additional affiliations
January 2020 - July 2020
Christian-Albrechts-Universität zu Kiel
Position
  • PostDoc Position
Education
January 2019 - July 2019
Aix-Marseille Université
Field of study
  • Animal experimentation
October 2016 - November 2019
Aix-Marseille Université
Field of study
  • Microbiology
September 2015 - July 2016
Université de Montpellier
Field of study
  • Microbiology and molecular gentics

Publications

Publications (38)
Article
Full-text available
Brucellosis is a bacterial zoonosis caused by bacteria of the genus Brucella spp. It is cosmopolitan and rages around the Mediterranean, particularly in Algeria where it continues to wreak havoc on livestock. We report here a review on brucellosis in Algeria, where human brucellosis was already discovered in 1895 and since then it remains a major c...
Article
Full-text available
Methanogens, the archaea uniquely detoxifying fermentative hydrogen into methane in the digestive tract, are increasingly being detected in pathology situations, rendering their rapid identification mandatory. We improved the experimental protocol to identify broth-cultured methanogens by matrix-assisted laser desorption time-of-flight MS (MALDI-TO...
Preprint
Full-text available
Background: Preterm birth is a major cause of morbidity and mortality in infants and children. Non-invasive methods for screening the neonatal immune status are lacking. Archaea, a prokaryotic life domain, comprise methanogenic species that are part of the neonatal human microbiota and contribute to early immune imprinting. However, they have not y...
Article
Full-text available
Agzul Tawla n Malṭa d aṭṭan ittenṭaḍen ɣer umdan i d-xeddment tbaktiriyin n tewsit Brucella spp. D aṭṭan amaḍlan u tella deg tmura n wagrakal am Lezzayer anda mazal txeddem axessar deg lmal. Ad d-nawi dagi tasɣunt tamatut ɣef tawla n Malṭa di Lezzayer, anda i tt-id-ufan yakan ɣer umdan seg 1895 u deg wass-nni mazal-itt d ugur ameqqran i yimeẓla n t...
Article
Full-text available
Tetragenococcus halophilus (T. halophilus) is a facultative anaerobic, coccus-shaped halophilic lactic acid-producing bacterium previously detected and cultured in various salty foods and credited for beneficial effects on human health. In this study, we investigated the presence of T. halophilus in human samples using a polyphasic approach includi...
Article
Full-text available
Background: Preterm birth is a major cause of morbidity and mortality in infants and children. Non-invasive methods for screening the neonatal immune status are lacking. Archaea, a prokaryotic life domain, comprise methanogenic species that are part of the neonatal human micro-biota and contribute to early immune imprinting. However, they have not...
Article
Full-text available
Background Nanoarchaeota, obligate symbiont of some environmental archaea with reduced genomes, have been described in marine thermal vent environments, yet never detected in hosts, including humans. Methods Here, using laboratory tools geared towards the detection of nanoarchaea including PCR-sequencing, WGS, microscopy and culture. Results We d...
Preprint
Full-text available
Methanogens, the archaea uniquely detoxifying fermentative hydrogen into methane in the digestive tract, are increasingly detected in pathology situations, rendering their rapid identification mandatory. We improved the experimental protocol to identify broth-cultured methanogens by matrix-assisted laser desorption time-of-flight mass spectrometry...
Preprint
Full-text available
Methanogens are detected in human gut from the first moments of life and there is a diversification of methanogens during infancy. However, the sources of acquisition of methanogens are not well elucidated. We therefore investigated 56 dairy products as potential sources of methanogens by applying molecular biology. In the presence of negative cont...
Article
The aetiology of appendicular abscess is predominantly microbial with aerobic and anaerobic bacteria from gut flora. In this study, by using specific laboratory tools, we co-detected Methanobrevibacter oralis and Methanobrevibacter smithii among a mixture of enterobacteria including Escherichia coli, Enterococcus faecium and Enterococcus avium in f...
Poster
Full-text available
The development of a new tool for reliable, rapid, and reproducible diagnosis of methanogens of clinical interest by MALDI-TOF-MS mass spectrometry
Poster
Full-text available
The use of a chemical method of hydrogen production in the isolation and culture of methanogens to find an alternative to the use of Bacteroides thetaiotaomicron as a source of hydrogen during the isolation and culture of methanogens
Article
Full-text available
To understand the dynamics of methanogens in the human intestinal microbiota, we investigated the presence of methanogens in meconium using a polyphasic approach including microscopy and PCR-sequencing in 33 meconium samples collected from 33 pre-term neonates, in accordance with current ethics regulation. In the presence of negative controls, 90.9...
Article
Full-text available
Untreated tonsillar phlegmon is a life-threatening condition commonly caused by Streptococcus pyogenes and Fusobacterium necrophorum among other pathogens. Here using specific laboratory tools, we detected Methanobrevibacter smithii in addition to S. pyogenes. This unprecedented observation questions the role of methanogens in phlegmon, and the opt...
Article
Full-text available
Methanogens are members of anaerobe microbiota of the digestive tract of mammals, including humans. However, the sources, modes of acquisition, and dynamics of digestive tract methanogens remain poorly investigated. In this study, we aimed to expand the spectrum of animals that could be sources of methanogens for humans by exploring methanogen carr...
Article
Full-text available
Methanogens are members of anaerobe microbiota of the digestive tract of mammals, including humans. However, the sources, modes of acquisition, and dynamics of digestive tract methanogens remain poorly investigated. In this study, we aimed to expand the spectrum of animals that could be sources of methanogens for humans by exploring methanogen carr...
Article
Full-text available
Methanogens, the sole microbes producing methane, are archaea commonly found in human anaerobic microbiota. Methanogens are emerging as opportunistic pathogens associated with dysbiosis and are also detected and cultured in anaerobic abscesses. Their presence in the respiratory tract is yet unknown. As a preliminary answer, prospective investigatio...
Preprint
Full-text available
Methanogens are being members of anaerobe microbiota of the digestive tract of both human and mammals. However, the sources, modes of acquisition and dynamics of digestive tract methanogens remain poorly investigated. In this study, we aimed to expand the spectrum of animals which could be sources of methanogens for human, by exploring methanogen c...
Article
Full-text available
Background The spectrum of infections caused by methanogens remains to be described. We searched for methanogens in the blood of febrile patients using specific tools. Methods Blood culture samples routinely collected in patients with fever were prospectively screened by specific PCR assays for methanogens. Positive samples were observed by autofl...
Article
Methanogens are the archaea most commonly found in humans, in particular in the digestive tract and are an integral part of the digestive microbiota. They are present in humans from the earliest moments of life and represent the only known source of methane production to date. They are notably detected in humans by microscopy, fluorescent in situ h...
Article
Full-text available
Archaeal sequences have been detected in human colostrum and milk, but no studies have determined whether living archaea are present in either of these fluids. Methanogenic archaea are neglected since they are not detected by usual molecular and culture methods. By using improved DNA detection protocols and microbial culture techniques associated w...
Article
Full-text available
Archaea, which form one of four domains of life alongside Eukarya, Bacteria, and giant viruses, have long been neglected as components of the human microbiota and potential opportunistic infectious pathogens. In this review, we focus on methanogenic Archaea, which rely on hydrogen for their metabolism and growth. On one hand, methanogenic Archaea i...
Article
Methanogen cultures require hydrogen produced by fermentative bacteria such as Bacteroides thetaiotaomicron (biological method). We developed an alternative method for hydrogen production using iron filings and acetic acid with the aim of cultivating methanogens more efficiently and more quickly (chemical method). We developed this new method with...
Article
Full-text available
Vaginosis is a dysbiotic condition of the vaginal cavity that has deleterious effects during pregnancy. The role of methanogens in this disease is unknown since current methods of investigation are not appropriate for the search of methanogens. We prospectively investigated the presence of methanogens in vaginal specimens collected from 33 women th...
Preprint
Methanogen cultures require hydrogen produced by fermentative bacteria such as Bacteroides thetaiotaomicron (biological method). We developed an alternative method for hydrogen production using iron filings and acetic acid with the aim of cultivating methanogens more efficiently and more quickly (chemical method). We developed this new method with...
Article
Full-text available
The microbial communities of the oral fluid are in direct contact with tobacco smoke, which may thus affect these communities. Few culture-based studies have analyzed the effects of tobacco smoking on the oral fluid microbiota. Using bacterial culture we investigated whether tobacco smoking altered the microbial diversity of the oral fluid, focusin...
Article
Full-text available
Background: Urinary tract infections are known to be caused by bacteria, but the potential implications of archaea have never been studied in this context. Methods: In two different university hospital centres we used specific laboratory methods for the detection and culture of archaeal methanogens in 383 urine specimens prospectively collected...
Article
Full-text available
Methanogens are strictly anaerobic archaea metabolising by-products of bacterial fermentation into methane by using three known metabolic pathways, i.e. the reduction of carbon dioxide, the fermentation of acetate or the dismutation of methanol or methylamines. Methanogens described in human microbiota include only Euryarchaeota, i.e. Methanobrevib...
Article
Full-text available
The salivary microbiome comprises an important bacterial diversity, yet the presence of archaea has not been reported so far. In order to quest for the presence of methanogenic archaea (methanogens) in saliva, we used a polyphasic approach including PCR-sequencing detection, microscopic observation by fluorescence in-situ hybridization, isolation a...
Article
Full-text available
Methanobrevibacter smithii is the main human methanogen almost always found in the digestive tract of adults. Yet, the age at which M. smithii establishes itself as part of the developing intestinal microflora remains unknown. In order to gain insight into this, we developed a polyphasic approach, including microscopic observation by fluorescence i...

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