
Geun-Bae KimChung-Ang University · School of Biological Sciences
Geun-Bae Kim
Ph.D.
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Introduction
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Publications
Publications (86)
The probiotic Bifidobacterium breve is known for its efficient conjugated linoleic acid (CLA) conversion, yet their CLA conversion pathway remains underexplored. This study investigated B. breve JKL2022 for its CLA conversion in actively growing cells, washed cell states, and in crude protein extracts. Moreover, the study aimed to confirm the CLA-c...
Ligilactobacillus salivarius harbors bacteriocin genes in its repA-type megaplasmid, specifically salivaricin P (salP), a class IIb bacteriocin. This study aimed to differentiate 25 salP-positive Lig. salivarius strains isolated from the gastrointestinal tract (GIT) of broilers and laying hens. Results showed that 12 isolates were classified as Typ...
Conjugated linoleic acid (CLA), a bioactive fatty acid that provides various physiological benefits, has gained increasing attention in the food industry, and various studies have focused on enhancing its content in dairy products. The factors influencing CLA content in dairy products vary significantly, including lactation stage, breed type, seaso...
Lactococcus lactis subsp. lactis DOME 6301, isolated from bovine milk, produces an antimicrobial compound that inhibits oral pathogens including Streptococcus mutans, Prevotella intermedia, and Fusobacterium nucleatum. The entire genome of L. lactis DOME 6301 was sequenced and assembled de novo using the PacBio RS II platform. The genome was 2,532,...
The efficacy and safety of galacto-oligosaccharides (GOS) in treating functional constipation were evaluated in a four-week randomized, double-blind clinical trial on 63 patients who met Rome IV criteria (34 GOS, 29 placebo group).
Enterococcus faecalis CAUM157 (KACC 81148BP), a Gram-positive bacteria isolated from raw cow’s milk, was studied for its bacteriocin production. The antimicrobial activity of CAUM157 was attributed to a two-peptide class IIb bacteriocin with potent activity against food-borne pathogen Listeria monocytogenes and periodontal disease-causing pathogens...
Ligilactobacillus agilis LDTM47 was isolated from gastric intestinal tract (ileum and jejunum) samples of broiler chickens from a farm associated with Chung-Ang University (Anseong, Korea). Ligilactobacillus are Gram-positive lactobacilli generally associated with the intestinal tracts of vertebrates. Members of lactic acid bacteria are considered...
Enterococcus faecalis CAUM157 (KACC 81148BP), a Gram-positive bacteria isolated from raw cow’s milk, was studied for its bacteriocin production. The antimicrobial activity of CAUM157 was attributed to a two-peptide Class IIb bacteriocin with potent activity against food-borne pathogen Listeria monocytogenes and periodontal disease-causing pathogens...
Background
A recent increase in the occurrence of canine skin and soft tissue infections, including otitis externa and pyoderma, caused by antimicrobial-resistant Staphylococcus pseudintermedius and S. schleiferi has become a significant public and veterinary health issues.
Objective
We investigated the virulence potentials associated with the occ...
Non-aureus staphylococci (NAS), particularly antimicrobial-resistant NAS, have a substantial impact on human and animal health. In the current study, we investigated (1) the species profiles of NAS isolates collected from healthy broilers, farm environments, and farm workers in Korea, (2) the occurrence of antimicrobial-resistant NAS isolates, espe...
Ligilactobacillus is a genus of Gram-positive lactobacilli commonly found in the intestinal tracts of vertebrates. It has been granted a Qualified Presumption of Safety (QPS) status from the European Food Safety Authority (EFSA). One specific strain, Ligilactobacillus salivarius B4311, was isolated from fecal samples of broiler chickens from a farm...
The objective of this study was to investigate the efficacy of whey bioconversion products (WBP) with Enterococcus faecalis M157 KACC81148BP on the improvement of periodontitis.
Effects of whey bioconversion products (M157) produced by Enterococcus faecalis M157 KACC81148BP and M157 supplemented with bacteriocin active fraction (M157+) on the perio...
In recent years, biocontrol of foodborne pathogens has become a concern in the food industry, owing to safety issues. Listeria monocytogenes is one of the foodborne pathogens that causes listeriosis. The major concern in the control of L. monocytogenes is its viability as it can survive in a wide range of environments. The purpose of this study was...
Colonization of food-producing animals by antimicrobial-resistant Staphylococcus aureus, especially methicillin-resistant S. aureus (MRSA), has become a serious public health problem worldwide. In the current study, clonal diversities of livestock-associated S. aureus isolates collected from broiler farms, slaughterhouses, and retail chicken meat w...
Chryseobacterium mulctrae KACC 21234T is a novel species isolated from raw bovine milk. Psychrotrophic bacteria are considered contaminants and are hypothesized to originate from the environment. In this investigation, the C. mulctrae KACC 21234T genome was determined to be 4,868,651 bp long and assembled into four contigs with a G+C ratio of 33.8%...
Sequence type (ST) 5 methicillin-resistant Staphylococcus aureus
(MRSA) with staphylococcal cassette chromosome mec (SCCmec)
type II (ST5-MRSA-II) and ST72-MRSA-IV represent the most significant genotypes
for healthcare- (HA) and community-associated (CA) MRSA in Korea, respectively.
In addition to the human-type MRSA strains, the prevalence of
liv...
The aim of the present study was to investigate the antiinflammatory and antibiofilm effects of whey fermented by Enterococcus faecalis M157 (M157-W) against oral pathogenic bacteria. The M157-W significantly inhibited IL-1β, IL-6, and nitric oxide induced by the lipopolysaccharide of Porphyromonas gingivalis in RAW 264.7 cells. The M157-W also inh...
Linezolid resistance, mediated by the cfr gene, which confers resistant phenotypes to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A antimicrobials, has emerged in S. aureus and non-aureus staphylococci (NAS). Moreover, due to the transferable potential via plasmids, the spread of cfr among staphylococci is of great co...
As commensal colonizers in livestock, there has been little attention on staphylococci, especially non-aureus staphylococci (NAS), contaminating meat production chain. To assess prevalence of staphylococci in retail pork and slaughterhouse carcass samples in Korea, we collected 578 samples from Korean slaughterhouses (n=311) and retail markets (n=2...
Clostridium perfringens B20 was isolated from chicken feces
collected from a local farm associated with Chung-Ang University (Anseong,
Korea). The whole genome of C. perfringens B20 was sequenced
using the PacBio RS II platform and assembled de novo. The
genome is 2,982,563 bp long and assembled in two contigs. Annotation analyses
revealed 2,668 pr...
This study developed postbiotics with whey bioconversion product produced by Enterococcus faecalis M157 KACC 81148BP, and mixed whey bioconversion products produced by E. faecalis M157 KACC 81148BP and Lactococcus lactis ssp. lactis CAU2013 KACC 81152BP to alleviate periodontitis (PD) and to improve gut health. The powdered whey bioconversion produ...
Enterococcus faecalis CAUM157, isolated from raw cow’s milk, is a Gram-positive, facultatively anaerobic, and non-spore-forming bacterium capable of inhabiting a wide range of environmental niches. E. faecalis CAUM157 was observed to produce a two-peptide bacteriocin that had a wide range of activity against several pathogens, including Listeria mo...
Strain CA7T, a Gram-stain-negative, non-motile, non-spore-forming, aerobic and rod-shaped bacterial strain, was isolated from raw cow's milk collected from a farm affiliated with Chung-Ang University, Anseong, Korea, and characterized by a polyphasic approach. Optimal growth of strain CA7T was observed on tryptic soy agar at 30 °C and pH 7.0 with 0...
A novel bacterial strain, named S23T, was isolated from chicken meat of local market in Korea. Cells were Gram-negative, milky-yellow colored, non-motile and coccobacillus. The strain was obligate aerobic and catalase-positive, oxidase-negative, optimum growth temperature and pH were 25 °C and pH 7.0, respectively. On the basis of 16S rRNA gene seq...
Streptococcus hyointestinalis B19 was isolated from chicken
feces collected from local farm in Anseong, Korea. S.
hyointestinalis B19 was shown to produce bacteriocin-like compounds
exhibiting inhibitory activities against several pathogens including strains of
Clostridium perfringens and Listeria
monocytogenes. The whole genome of S.
hyointestinal...
A bacterial strain, designated B301T, isolated from raw chicken meat obtained from a local market in Korea, was characterized and identified using a polyphasic taxonomy approach. Cells were Gram-stain-negative, non-motile, obligate-aerobic coccobacilli, catalase-positive, and oxidase-negative. The optimum growth conditions were 30°C, pH 7.0, and 0%...
A Gram-stain-negative bacterial strain, designated CA10T, was isolated from bovine raw milk sampled in Anseong, Republic of Korea. Cells were yellow-pigmented, aerobic, non-motile bacilli and grew optimally at 30 °C and pH 7.0 on tryptic soy agar without supplementation of NaCl. Phylogenetic analysis based on the 16S rRNA gene sequences revealed th...
This study aimed to screen lactic acid bacteria (LAB) for their anti-inflammatory activity by using RAW264.7 cells and dextran sulfate sodium (DSS)-induced colitis. In all, 192 LAB strains were isolated from healthy human feces, of which 8 strains showed excellent nitric oxide (NO) inhibitory activity. Peptidoglycan extracts of these 8 LAB strains...
A Gram-stain-negative, yellow-pigmented, non-motile, non-spore-forming, aerobic and rod-shaped bacterial strain, designated 17S1E7T, was isolated from the Han River, Republic of Korea, and characterized by polyphasic taxonomy analyses. Strain 17S1E7T grew optimally on tryptic soy agar at 37 °C and pH 7.0 in the absence of NaCl. Phylogenetic analysi...
A common belief is that human milk is sterile. However, the development of culture-independent molecular methods, especially Next Generation Sequencing, has revealed that human milk harbors diverse and rich bacterial communities. Although studies aimed at characterizing the microbiota of human milk have produced different findings, Staphylococcus a...
Objective:
This study was conducted to identify duck liver-expressed antimicrobial peptide 2 (LEAP-2) and demonstrate its antimicrobial activity against various pathogens.
Methods:
Tissue samples were collected from 6-8-week-old Pekin duck (Anas platyrhynchos domesticus), total RNA was extracted and cDNA was synthesized. To confirm the duck LEAP...
This study was performed to isolate some strains of Bifidobacteriumbreve from fecal materials of neonates and to screen them for the biotransformation activity of converting linoleic acid into conjugated linoleic acid (CLA). Fecal samples were collected from twenty healthy neonates between 14 and 100 days old, and four hundred colonies were randoml...
In this study, we isolated and characterized a bacteriocin-producing strain and two bacteriophages (P4, A3), showing antimicrobial effects against Clostridium perfringens, from chicken and swine feces by the spot-on-the lawn antagonism method. The selected strain was identified as Streptococcus hyointestinalis by 16S rRNA gene sequencing. The bacte...
Spermatogonial stem cells (SSCs) are the basis of spermatogenesis, which is dependent on the ability to self-renew and differentiation. Controlling self-renewal and differentiation of SSCs could apply to treatment of disease such as male infertility. Recently, in the field of stem cell research, it was demonstrated that effective increase in stem c...
This study investigated the psychrotrophic bacteria isolated from chicken meat to characterize their microbial composition during refrigerated storage. The bacterial community was identified by the Illumina MiSeq method based on bacterial DNA extracted from spoiled chicken meat. Molecular identification of the isolated psychrotrophic bacteria was c...
In this study, two Listeria bacteriophages, LMP1 and LMP7, were isolated from chicken feces as a means of biocontrol of L. monocytogenes. Both bacteriophages had a lytic effect on L. monocytogenes ATCC 7644, 15313, 19114, and 19115. Phages LMP1 and LMP7 were able to inhibit the growth of L. monocytogenes ATCC 7644 and 19114 in tryptic soy broth at...
Background:
Active packaging refers to the mixing of additive agents into packagingmaterials with the purpose of hold or extending food product quality and shelf life. The purpose of this study was to develop an easy and cheap active packaging in beef. Beef loin samples were divided into 3 packaging groups (C: ziplock bag-packaging, T1: vacuum-pac...
The aim of this study was to compare the sequence of the astA gene found in 8 Korean and 11 Japanese Escherichia coli isolates. Conventional PCR was used to amplify the astA gene from the chromosomal and plasmid DNA preparation samples of each isolate using commercial DNA extraction kits. Cloning of the PCR products, sequence analysis, and pulse fi...
To evaluate the effects of lactic acid bacteria (LAB) on Peyer's patch (PP) cells in mice treated with a high dose of kanamycin to disturb the gut microbial environment. The overarching goal was to explore the potential of LAB for use as a dietary probiotic that buffers the negative consequences of antibiotic treatment. In vitro, LAB stimulated the...
Three commercial broiler breeds were fed from hatch with a diet supplemented with Capsicum and Curcuma longa oleoresins, and co-infected with Eimeria maxima and Clostridium perfringens to induce necrotic enteritis (NE). Pyrotag deep sequencing of bacterial 16S rRNA showed that gut microbiota compositions were quite distinct depending on the broiler...
We investigated the effect of consuming probiotic fermented milk (PFM) on the microbial community structure in the human intestinal tract by using high-throughput barcoded pyrosequencing. Six healthy adults ingested 2 servings of PFM daily for 3 wk, and their fecal microbiota were analyzed before and after 3 wk of PFM ingestion period and for anoth...
The present study was conducted to screen candidate probiotic strains for anti-inflammatory activity. Initially, a nitric oxide (NO) assay was used to test selected candidate probiotic strains for anti-inflammatory activity in cultures of the murine macrophage cell line, RAW 264.7. Then, the in vitro probiotic properties of the strains, including b...
Abstract 1. Bacteriophages (BP) have gained increasing attention as a treatment of bacterial infection for animals. However, the data pertaining to dietary application of BP for laying hens have been limited. 2. This study aimed to investigate the effect of dietary BP on laying performance, egg quality, and caecal bacterial populations in laying he...
This experiment was conducted to investigate the effect of dietary bacteriophage (BP) on growth performance and cecal bacterial populations in broiler chickens raised in different housing systems. A total of 1,170 1-d-old broiler chickens were housed in either battery cages (120 birds) or conventional floor pens (1,050 birds). Within each housing s...
This study provides an overview of the factors that influence the effect of fermentation on the antioxidant activity and the mechanisms that augment antioxidative activities in fermented plant-based foods. The ability of fermentation to improve antioxidant activity is primarily due to an increase in the amount of phenolic compounds and flavonoids d...
A novel Bifidobacterium, designated strain CU3-7T, was isolated from two-weeks-old baby feces. The isolate was Gram-staining-positive, anaerobic and rod-shaped. Results from 16S rRNA gene sequence analysis revealed that strain CU3-7T was phylogenetically affiliated with members of the genus Bifidobacterium. Strain CU3-7T showed the highest level of...
Bacterial communities in the different regions of gastrointestinal tract (GIT) of broiler chickens were analyzed by pyrosequencing approach to understand microbial composition and diversity. The DNA samples extracted from 7 different regions along the GIT were subjected to bacterial-community analysis by pyrosequencing of the V1-V3 region of 16S rR...
Aims:
The aim of this study was to evaluate the effects of Bifidobacterium lactis HY8101 on insulin resistance induced using tumour necrosis factor-α (TNF-α) in rat L6 skeletal muscle cells and on the KK-A(Y) mouse noninsulin-dependent diabetes mellitus (NIDDM) model.
Methods and results:
The treatment using HY8101 improved the insulin-stimulate...
Spermatogonial xenotransplantation assay provides access to the several species germline and has been used in experimental animal models to study stem cell biology and germline development. To make the xenotransplantation technology more extensively accessible, it is require development of recipient preparation protocols. Therefore, we propose an o...
The objective of this experiment was to investigate the effect of dietary supplementation of Lactobacillus-fermented Artemisia princeps (LFA) on growth performance, meat lipid peroxidation, and intestinal microflora in Hy-line Brown male chickens. A total of
six hundred twenty-four 1-d-old Hy-Line Brown male chicks were randomly allotted to 3 dieta...
As an appraisal for the application of a new starter culture, more than 200 lactic acid bacteria strains were isolated from raw milk and healthy human feces. The strains showing excellent growth and acid production ability in 10% skim milk media were selected and identified as Lactobacillus casei based on the results of their API carbohydrate ferme...
Aims:
To clone, characterize and compare the bile salt hydrolase (BSH) genes of Lactobacillus johnsonii PF01.
Methods and results:
The BSH genes were amplified by polymerase chain reaction (PCR) using specific oligonucleotide primers, and the products were inserted into the pET21b expression vector. Escherichia coli BLR (DE3) cells were transfor...
Lactobacillus mucosae LM1, isolated from stool samples of a healthy piglet, displays good in vitro mucin adhesion and antimicrobial activity against pathogenic bacteria. To elucidate its antimicrobial effects and to find
its epithelial cell and mucin adhesion genes, the genomic sequence of L. mucosae LM1 was investigated.
A genetically modified (GM) pepper containing the gene for the cucumber mosaic virus (CMV) coat protein (CP) was investigated to assess the proteolytic behavior and simulated digestion in the stomach and small intestine. CMV-CP expressed in GM pepper and in Escherichia coli exhibited the molecular mass of about 25 kDa on sodium dodecyl sulphate-pol...
Strain R54(T) was isolated from the gizzard of hens. The isolate was Gram-positive, facultative anaerobic, gas-forming, catalase-negative, nonmotile, nonspore-forming and short-rod-shaped. The optimal temperature for growth was 40 °C and the DNA G+C content was 42.7 mol%. The 16S rRNA gene sequences similarity showed that strain R54(T) was most clo...
Lactobacillus salivarius is a well-known lactic acid bacterium to which increasing attention has been paid recently for use as probiotics for humans
and animals. L. salivarius NIAS840 was first isolated from broiler chicken feces, displaying antimicrobial activities against multidrug-resistant Staphylococcus aureus and Salmonella enterica serovar T...
Lactobacillus johnsonii PF01, an autochthonous bacterium of the gastrointestinal tract, was isolated from a fecal sample from a piglet. The strain
adhered specifically to the duodenal and jejunal epithelial cells of the piglet and had high bile resistance activity. Here
we report the genomic sequence of L. johnsonii PF01.
The draft genome sequence of Lactobacillus salivarius GJ-24 isolated from the feces of healthy adults was determined. Its properties, including milk fermentation activity and
bacteriocin production, suggest its potential uses as a probiotic lactic acid bacterium and start culture for dairy products.
We investigated the improvement in intestinal function and the constipation preventive and remedial effects of the fermented milk product R&B in mice and rats. The results showed that the effects varied significantly among different doses. The digestive tract transfer rate increased dramatically in a dose-dependent manner in both animals. Compared...
In this study, we investigated the effect of dietary supplementation of Cu-Met chelate (Cu-Met) and Cu-soy proteinate (Cu-SP) on the performance, blood parameters, liver mineral content, and intestinal microflora in broiler chickens. A total of 1,008 hatched Ross broiler chickens were randomly assigned to 1 of 6 dietary treatments (T): T1, control;...
This study investigated the effects of dietary supplementation with the prebiotics fructo-oligosaccharide (FOS) and mannan-oligosaccharide (MOS) on the performance, small intestinal microflora, and immune response of broilers. Two hundred forty 1-d-old Ross broiler chickens were randomly assigned to 6 dietary treatment groups: control, avilamycin (...
This study was conducted to investigate the effects of dietary supplementation of multiple probiotics on the performance, small intestinal microflora and immune response in laying hens and broilers. In Exp.1, a total of 800, 82 wk old Hy-line Brown laying hens were assigned to one of the following five dietary treatment; Control, Antibiotics (avila...
As a trial for the development of a new starter culture for yogurt products, more than two hundred lactic acid bacteria strains were isolated from raw milk and healthy human feces. The strains that showed excellent growth and acid production ability in the 10% skim milk media were selected and identified as Lactobacillus casei through the API carbo...
This paper reports the results of the research on the effects of probiotic yogurt on the microbiological quality, pH, and sensorial characteristics of fresh chicken meat when packed with probiotic yogurt. The chicken meat pieces were packed with yogurt and were stored at 10°C for 7 days. Samples were taken after 0, 2,4, and 7 days of storage, and w...
To clone, sequence and characterize a new bile salt hydrolase from a bile tolerant strain of Bifidobacterium animalis ssp. lactis KL612, and further analysis of the bsh promoter and an operon-like structure containing the bsh gene in the genus Bifidobacterium.
A new type of bile salt hydrolase from a bile tolerant strain of Bifidobacterium was clon...
Phenotypic screening for bile salt hydrolase (BSH) activity was performed on Lactobacillus acidophilus PF01 isolated from piglet feces. A gene encoding BSH was identified and cloned from the genomic library of L. acidophilus PF01. The bsh gene and surrounding regions were characterized by nucleotide sequence analysis and were found to contain a sin...
The distributions and content of geometrical isomers of conjugated linoleic acids (CLA) in dairy foods such as milk, yogurt, and cheese, produced or being sold in the Quebec province of Canada, were investigated by gas chromatographic analysis. The mean contents of total CLA (mg/g fat) were 5.06±0.74 in 4 low-fat milk samples, 14.14 ± 4.95 in 6 yog...
The lactase activities of nine species of lactic acid bacteria were compared using the chromogenic substrate, . Streptococcus thermophilus KACC 91147 had the highest lactase activity among a total of thirty strains of Lactobacillus and S. thermophilus tested, including commercial strains. S. thermophilus KACC 91147 released of galactose in treated...
T-cell depleting anti-CD3 immunotoxins have utility in non-human primate models of transplantation tolerance and autoimmune disease therapy. We recently reported that an affinity matured single-chain (scFv) anti-monkey CD3 antibody, C207, had increased binding to T-cells and increased bioactivity in a diphtheria toxin (DT)-based biscFv immunotoxin...
The bile salt hydrolysis is one of the most important metabolic activities that are exhibited by enteric bacteria colonizing in the Gl tract of humans and animals. The BSH active probiotic strains such as Bifidobacterium animalis-ssp-lactis are commercially used as probiotic yogurts and capsule forms that may bring about a lowering of serum cholest...
Recently, a bivalent recombinant anti-human CD3 diphtheria toxin (DT) based immunotoxin derived from the scFv of UCHT1 antibody has been made that shows enhanced bioactivity and is free from the side effects of Fc receptor interaction. In this case, the diminution of CD3 binding due to the placement of the scFv domain at the C-terminus of the trunc...
Recombinant beta-galactosidase from Bifidobacterium longum LL04 was expressed in Escherichia coli and partially purified by ammonium sulphate precipitation and anion-exchange chromatography (Mono-Q). The optimum temperature and pH of the partially purified enzyme were 50 degrees C and pH 7.0-8.0, respectively, when o-nitrophenyl-beta-D-galactopyran...
Extraction properties of different solvents (chloroform/methanol, hexane/isopropanol, and hexane) were studied for the gas chromatographic analysis of conjugated linoleic acids (CLA) from probiotic bacteria grown in de Man, Rogosa, and Sharpe medium. As compared with chloroform/methanol and hexane/isopropanol, hexane showed comparable extraction ef...
Bile salt deconjugation is the most biologically significant reaction among the bacterial alterations of bile acids in the gastrointestinal tract of human and animal. The responsible enzyme, bile salt hydrolase (BSH), catalyzes the hydrolysis of glycineand/or taurine-conjugated bile salts into amino acid residues and deconjugated bile acids. Herein...