Getnet Midekessa

University of Tartu · Institute of Biomedicine and Translational Medicine

Introduction Extracellular vesicles (EVs) facilitate inter and intra-species/kingdom communication through biomolecule transfer, including proteins and small RNAs. Plant-derived EVs, a hot topic in the field, hold immense capability both as a potential biomarker to study plant physiology and as a biomaterial that can be mass-produced to be used in...

Inhibitory Effects of Chlorella vulgaris Algasomes on Listeria species Growth and Biofilm Eradication Dulmini Nanayakkara Sapugahawatte1Ɨ, Kasun Godakumara1 Ɨ, Gayandi Ekanayake1, Deep Bhattacharya1, Getnet Balcha Midekessa1,2, Mati Roasto3, Renu Geetha Bai4, Timo Kikas4, Aneta Andronowska5, Alireza Fazeli1,2,6* 1. Institute of Veterinary Medicin...

Background Bacillus cereus is commonly found in many foods and known for causing foodborne illness. Due to their spore-forming and biofilm-forming nature and contamination capability these bacteria are presenting a large challenge to the food industry. The bacterium has become increasingly resistant to multiple drugs necessitating novel preventive...

Background Bacillus cereus is commonly found in many foods and known for causing foodborne illness. Due to their spore-forming and biofilm-forming nature and contamination capability these bacteria are presenting a large challenge to the food industry. The bacterium has become increasingly resistant to multiple drugs necessitating novel preventive...

Effective suppression of Staphylococcus aureus ATCC 25923 growth through periodic dosing of pasteurized milk-derived extracellular vesicle Dulmini Nanayakkara Sapugahawtte1, Kasun Godakumara1, Mihkel Mäesaar2, Gayandi Ekanayake1, Getnet Balcha Midekessa1,3, Madhusha Prasadini1, Suranga Kodithuwakku1,4, Mati Roasto2, Aneta Andronowska5, Alireza Faze...

Background/ hypothesis Bovine mastitis, a global dairy cattle disease, impacts animal health, milk quality and public health due to its zoonotic potential. Staphylococcus aureus, Streptococcus agalactiae (GBS), and Escherichia coli are the key mastitis-causing pathogens. This study examined whey and algae spent media-derived extracellular vesicles...

The growing understanding of the role of extracellular vesicles (EVs) in embryo-maternal communication has sparked considerable interest in their therapeutic potential within assisted reproductive technology, particularly in enhancing implantation success. However, the major obstacle remains the large-scale production of EVs, and there is still a g...

Increasing antimicrobial resistance (AMR) challenges conventional antibiotics, prompting the search for alternatives. Extracellular vesicles (EVs) from pasteurised cattle milk offer promise, due to their unique properties. This study investigates their efficacy against five pathogenic bacteria, including Staphylococcus aureus ATCC 25923, aiming to...

Extracellular vesicles (EVs) are released by cells, and have a lipid bilayer structure. EVs harbor various molecules, including proteins, RNAs, and DNAs. Studies of mammalian EVs are increasingly attracting the interest of researchers; however, there are few studies of nanoparticles in food. Milk-derived EVs can survive high temperatures and digest...

Extracellular vesicles (EVs) are membrane-bound nanoparticles that are released by different cell types and play a crucial role in the intercellular communication. They carry various biomolecular compounds such as DNA, RNA, proteins, and lipids. Given that EVs are a new element of the communication within the ovarian follicle, extensive research is...

Extracellular vesicles (EV) have been identified in uterine fluid (UF), however the bovine UF-EV profile during different phases of the oestrous cycle has not yet been established. Therefore, we compared the UF-EV, and their protein profile at follicular and luteal phases of the oestrous cycle. UF samples were collected from healthy uteri of six li...

Extracellular vesicles (EVs), play a crucial role in mammalian cell-to-cell communication, by transporting components such as proteins and small RNAs. Similarly, plants are also believed to be producing EVs under various circumstances. Herein, we attempted to purify and characterize vesicle-like structures separately, from initial sampling solution...

This is the lab hand book of the EV training school- 2022. Contributors; Kasun Godakumara , Gayandi Ekanayaka, Qurat Ul Ain , Madhusha Prasadani, Getnet Midekessa , Omidreza Mousavi, Keerthi Dissanayaka , Subhashini Muhandiram, Mehedi Hasan, Johanna Piibor, Chanaka Premathilaka https://dspace.emu.ee/handle/10492/7997

Psoriasis vulgaris (PsV) and psoriatic arthritis (PsA) are inflammatory diseases with unresolved pathophysiological aspects. Extracellular vesicles (EVs) play an important role in intercellular communication. We compared the miRNA contents and surface proteome of the EVs in the blood serum of PsV and PsA patients to healthy controls. Size-exclusion...

Research on extracellular vesicles (EVs) has intensified over the past decade, including fluorescent membrane labeling of EVs. An optimal fluorescent method requires the size of EVs to be preserved after labeling. Lipophilic fluorescent dyes, such as CellMask™ Green (CMG), have been widely used for this purpose. Here, we investigated conditions aff...

Extracellular vesicles (EVs) are membrane-bound nanoparticles that are secreted by most cell types with an emerging role in cellular communication and potential as biomarkers of disease. Nanoparticle tracking analysis (NTA) is a commonly used technique to measure the size and concentration of nanoparticles, such as EVs. Here, we present two protoco...

Extracellular vesicles (EVs), including exosomes and microvesicles (<200 nm), play a vital role in intercellular communication and carry a net negative surface charge under physiological conditions. Zeta potential (ZP) is a popular method to measure the surface potential of EVs, while used as an indicator of surface charge, and colloidal stability...

Extracellular vesicles (EVs) are membrane-bound biological nanoparticles (NPs) and have gained wide attention as potential biomarkers. We aimed to isolate and characterize EVs from media conditioned by individually cultured preimplantation bovine embryos and to assess their relationship with embryo quality. Presumptive zygotes were cultured individ...

Multifunctional enzyme, type‐1 (MFE1) is a monomeric enzyme with a 2E‐enoyl‐CoA hydratase and a 3S‐hydroxyacyl‐CoA dehydrogenase (HAD) active site. Enzyme kinetic data of rat peroxisomal MFE1 show that the catalytic efficiencies for converting the short‐chain substrate 2E‐butenoyl‐CoA into acetoacetyl‐CoA are much lower when compared with those of...