Gerhard Gaedicke
In memory of

Gerhard Gaedicke
Medizinische Universität Innsbruck · Department of Pediatrics I

MD

About

242
Publications
13,819
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4,682
Citations
Citations since 2017
0 Research Items
667 Citations
2017201820192020202120222023020406080100120
2017201820192020202120222023020406080100120
2017201820192020202120222023020406080100120
2017201820192020202120222023020406080100120
Additional affiliations
August 1993 - October 2011
Charité Universitätsmedizin Berlin
Position
  • Professor (Full) of Paediatrics, Director Dept. of Paediatrics, 1993-2009
August 1993 - September 2009
Humboldt-Universität zu Berlin
Position
  • Full Professor of Paediatrics
Description
  • Head Dept. of Pediatrics

Publications

Publications (242)
Article
Much has been written about the patient–physician relationship over the years. This relationship is essential in maintaining trust in the complex arena of modern diagnostic techniques, treatment and prevention, including vaccines and vaccine safety. However, a great deal of this material was written from the viewpoint of clinicians and academics. T...
Article
Full-text available
Not only the general public, but also those studying to become health professionals, are struggling to keep up with a growing body of evidence and increasingly complex information about the many different types of vaccines available to date. At the same time, a number of increasingly complex subjects of study are competing for their attention durin...
Article
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The highly complex and controversial topic of vaccine safety communication warrants innovative, user-centered solutions that would start with gaining mutual respect while taking into account the needs, concerns and underlying motives of patients, parents and physicians. To this end, a non-profit collaborative project was conducted by The Vienna Vac...
Article
The highly complex and controversial topic of vaccine safety communication warrants innovative, user-centered solutions that would start with gaining mutual respect while taking into account the needs, concerns and underlying motives of patients, parents and physicians. To this end, a non-profit collaborative project was conducted by The Vienna Vac...
Article
Objectives: Vaccine safety surveillance is highly dependent on accurate reporting of adverse events following immunization (AEFI). An online survey was conducted to assess the utilization of AEFI reporting standards and pathways among pediatricians in Germany, and in Russia where pediatric specialization begins in medical school. Methods: In May...
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Full-text available
Background: The Kids ITP Tools (KIT) is a disease-specific measure of health-related quality of life for children with immune thrombocytopenia (ITP). To facilitate use in international trials it has been cross-culturally adapted for France, Germany, the United Kingdom and Uruguay. This study assessed the validity and reliability of the translated...
Article
Platelet counts below normal values define thrombocytopenia. However, platelet counts alone do not reveal the underlying pathomechanism. New blood cell counters provide additional information on platelet size and volume, and enable the distinction of sub-populations. In this preliminary study, we evaluate whether one of these markers can be used fo...
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Thrombocytopenia with absent radii syndrome is defined by bilateral radius aplasia and thrombocytopenia. Due to impaired thrombopoietin signaling there are only few bone marrow megakaryocytes and these are immature; the resulting platelet production defect improves somewhat over time. A microdeletion on chromosome 1q21 is present in all patients bu...
Article
Neuroblastoma (NB) is the most common solid extracranial tumor in childhood. High-level expression of MycN caused by amplification of the gene plays an important role in maintaining the malignant phenotype of NB and is the most significant marker for poor prognosis overall. Recent studies suggested that MYCN is a suitable target for immunotherapy,...
Article
Background: Neuroblastoma (NB) is an aggressive neoplasm of the sympathetic nervous system in early childhood. Current treatment of high-risk NB consists of intensive myeloablative therapy followed by bone marrow transplantation. However, children with advanced stage disease or those with refractory disease have a poor prognosis. A promising adjuva...
Article
To investigate the applicability, efficacy, and safety of single-pass albumin dialysis in children. Retrospective data review of uncontrolled clinical data. University-based pediatric intensive care unit collaborating with a local center for liver transplantation. Nine children, aged 2 to 15 yrs, who were treated with single-pass albumin dialysis f...
Article
Patients, physicians and insurance underwriters are time and again confronted with procedures which they must deal with and where the orthodox school of medicine is of the opinion that their use is not or insufficiently substantiated. These methods are of special importance for patients with cancer. Every physician who is involved in oncology must,...
Article
2501 Poster Board II-478 Introduction There is considerable controversy surrounding whether or not children with Immune Thrombocytopenic Purpura (ITP) should be treated if they present without bleeding. One of the potential benefits of treatment would be to improve the child's health-related quality of life (HRQoL). Patients and Methods Variables...
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Neuroblastoma (NB) is a challenging malignancy of the sympathetic nervous tissue characterized by a very poor prognosis. One important marker for NB is the expression of tyrosine hydroxylase (TH), the first-step enzyme of catecholamine biosynthesis. We could show stable and high TH gene expression in 67 NB samples independent of the clinical stage....
Article
The likelihood of identifying peptides of sufficient quality for the development of effective cancer vaccines by screening of phage display libraries is low. Here, we introduce the sequential application of systematic amino acid substitution by SPOT synthesis. After the substitution of two amino acids within the sequence of a phage display-derived...
Article
Children with relapsed and refractory acute lymphoblastic leukemia (ALL) still face a critical prognosis. We tested the hypothesis that targeted calicheamicin theta (θ) using an anti-CD19-immunoconjugate may provide an effective treatment strategy for CD19(+) ALL. Calicheamicin θ is a rationally designed prodrug of the natural enediyene calicheamic...
Article
The inhibitor of apoptosis protein survivin is highly expressed in neuroblastoma (NB) and survivin-specific T cells were identified in Stage 4 patients. Therefore, we generated a novel survivin minigene DNA vaccine (pUS-high) encoding exclusively for survivin-derived peptides with superior MHC class I (H2-K(k)) binding affinities and tested its eff...
Article
Neuroblastoma (NB) and melanoma are neuroectodermal tumors characterized by poor prognosis. The T cell independent antigen ganglioside GD2 is highly expressed on most NB and on 30% of melanomas, which makes it an interesting target for immunotherapeutic approaches. NK cells are able to actively lyse tumors with downregulated MHC class I expression...
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Der studentische Unterricht in der Kinderheilkunde an der Charité hat seit 1999 mehrere Umstrukturierungen durchlaufen. Mit der Einführung der neuen ärztlichen Approbationsordnung 2004 im Regelstudiengang (RSG) und dem Modellcurriculum Reformstudiengang Medizin (RSM) 1999 müssen zwei Curricula parallel neu konzipiert und gepflegt werden. Durch den...
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Therapeutic vaccination against tumor antigens without induction of autoimmunity remains a major challenge in cancer immunotherapy. Here, we show for the first time effective therapeutic vaccination followed by suppression of established spontaneous neuroblastoma metastases using a tyrosine hydroxylase (TH) DNA minigene vaccine. We identified three...
Article
Zahlreiche Fakultäten weltweit setzen die Unterrichtsform des problemorientierten Lernens (POL) in ihren Curricula ein, bei der Studierende in Kleingruppen – ausgehend von einem konkreten Fall – Problemlösestrategien entwickeln. Hierbei wird nach definierten Arbeitsschritten, dem so genannten POL-Bogen, vorgegangen. Anders als beim klassischen Fron...
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Full-text available
The pediatric curricula in Munich, Heidelberg, Freiburg and Berlin represent a broad spectrum ranging from a traditional (Freiburg) to a reformed curriculum (Reformed Medical Studies Program Berlin). Special conditions pre-existed in Munich (Munich-Harvard-Alliance) and Heidelberg (HeiCuMed). All curricula are based on explicit learning goals and o...
Article
Primary ciliary dyskinesia (PCD) is an inherited disorder characterized by perturbed or absent beating of motile cilia, which is referred to as Kartagener syndrome (KS) when associated with situs inversus. We present a German family in which five individuals have PCD and one has KS. PCD was confirmed by analysis of native and cultured respiratory c...
Article
The replacement of established evidence-based cancer therapy protocols (mainstream therapy) by unevaluated complementary and alternative medicine (CAM) is a challenge in pediatric oncology. We tested the hypothesis that oral application of L-lysine and ascorbic acid (Lysin C Drink) in combination with epigallocatechin-gallate (EGCG) and amino-acids...
Article
We report the uncommon clinical course of tetanus in a completely immunized 14-year-old boy. His initial symptoms, which included a flaccid paralysis, supported a diagnosis of botulism. Preliminary mouse-test results with combined botulinum antitoxins A, B, and E, obtained from tetanus-immunized horses, backed this diagnosis. The change in his clin...
Article
9521 Background: Fractalkine (FKN) is a unique CX3C chemokine (CX3CL1) known to induce adhesion and migration of leukocytes mediated by a membrane-bound and a soluble form. Methods: We found that FKN is expressed in >90% of 68 neuroblastoma (NB) samples as determined by cDNA microarray analysis. FKN expression was inversely correlated with MYCN amp...
Article
The development of pulmonary arterial hypertension (PAH) is the leading cause of mortality in patients with thalassemia and sickle cell anemia and was reported to occur in hemolytic anemias such as hereditary stomatocytosis, and paroxysmal nocturnal hemoglobinuria. Here, we report for the first time on the development of PAH in a patient with Hb-Ma...
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Full-text available
Fractalkine (FKN) is a unique CX3C chemokine (CX3CL1) known to induce both adhesion and migration of leukocytes mediated by a membrane-bound and a soluble form, respectively. Its function is mediated through CX3C receptor (CX3CR), which is expressed by T(H)1 immune cells including T cells and natural killer (NK) cells. FKN was shown to be expressed...
Article
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Disialoganglioside GD2 is an established target for immunotherapy in neuroblastoma. We tested the hypothesis that active immunization against the glycolipid GD2 using DNA vaccines encoding for cyclic GD2-mimicking decapeptides (i.e., GD2 mimotopes) is effective against neuroblastoma. For this purpose, two GD2 peptide mimotopes (MA and MD) were sele...
Article
The cause of idiopathic thrombocytopenia (ITP) is largely unknown, although the underlying pathophysology is an autoimmune process. Anti-idiotypic antibodies and their role on regulatory T-cells might play an important role in the switch from acute to chronic ITP. The exact interaction remains to be elucidated. The effects of the dysregulated immun...
Article
The administration of intravenous immunoglobulins, IVIG, has lead to therapeutic success in a variety of autoimmune disorders in both children and adults. The authors of this review try to summarize our current knowledge on mechanism of action and clinical results of IVIG in a classical autoimmune disease, immune thrombocytopenia, ITP.
Article
The cells from 87 leukemia–lymphoma cell lines, 14 B-lymphoblastoid cell lines, 459 cases of leukemia–lymphoma, normal specimens, 22 leukemia–lymphoma cell lines treated with 12-O-tetradecanoylphorbol 13-acetate (TPA) and 14 cases of chronic lymphocytic leukemia (CLL) and chronic myelocytic leukemia (CML) treated with TPA were analyzed for the expr...
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Hmorrhagische Diathesen durch Thrombozytopenien umfassen verschiedenste Krankheitsentitten. Genauso vielfltig wie die Ursachen sind die Blutungsmanifestationen. Nach deren Intensitt und nach der Grundkrankheit richtet sich die Indikation zur Behandlung und die Wahl der Mittel. Thrombozytentransfusionen sind heute ein unverzichtbarer Bestandteil der...
Article
Intracellular antibodies (intrabodies) have been used for the generation of phenotypic knockouts in vivo by surface depletion of extracellular or transmembrane proteins. Intrabodies present an alternative to methods of gene inactivation that target genomic DNA or m-RNA, such as RNA interference. Several studies suggest that the VEGF receptor pathwa...
Article
Thrombozyten werden aus dem Zytoplasma von Megakaryozyten freigesetzt und haben eine entscheidende Funktion bei der Blutgerinnung. Sie sind auch in entzndliche Prozesse und die Wundheilung involviert. Die Proliferation und Differenzierung von Megakaryozyten wird durch die Bindung des Wachstumsfaktors Thrombopoietin (Tpo) an seinen Rezeptor c-Mpl st...
Chapter
Der 8-jährige Junge war mit dem Fahrrad gestürzt und hat sich die Lenkstange in die linke Flanke gestoßen. Bei der Aufnahme in der Klinik klagt er über Schmerzen unter dem linken Rippenbogen, besonders beim Einatmen. Blutdruck und Puls sind stabil, der Hb beträgt 13,8 g/dl. Eine Hämaturie besteht nicht. In der Sonographie zeigt sich ein großes, sub...
Chapter
Die Ursache der idiopathischen thrombozytopenischen Purpura (ITP) ist noch weitgehend unbekannt. Die Pathophysiologie ist (auto-)immunologisch. Erkenntnisse der veränderten Kaskade der Immunantwort führten zu modernen biologischen Therapieansätzen. Prospektive kooperative Studien zeigen neue Aspekte der Demographie, des Verlaufs und der Betreuung b...
Article
Die autoimmunhämolytischen Anämien sind bei Kindern selten. Wenn sie auftreten, sind sie meistens durch eine akute und schwere Hämolyse gekennzeichnet. Wichtigster diagnostischer Test ist neben dem Blutbild der positive direkte Coombs-Test. Nach dem Reaktionsoptimum der Autoantikörper unterscheidet man Wärmeautoantikörper und Kälteautoantikörper. I...
Article
»Wann wirst Du wieder transfundiert?« fragte die Rechenlehrerin den Viertklässler. »Am Montag« war die Antwort des Kindes. »Gut, dann schreiben wir die Klassenarbeit nächste Woche Mittwoch« erklärte die Lehrerin der am Ende der Stunde schon lärmenden Kinderschar. Auch wenn sich Kinder mit chronischer Anämie erstaunlich gut an niedrige Hämoglobinwer...
Article
Der Mutter eines 13-jähriges Mädchen fällt zunehmend die ständige Müdigkeit und Unkonzentriertheit bei der Tochter auf; auch die Schulleistungen sind in der letzten Zeit schlechter geworden. Eine aktuelle Untersuchung zeigt ein leicht untergewichtiges Mädchen mit blasser Gesichtsfarbe und Mundwinkelrhagaden sowie folgende Blutwerte: Hb 9,8 g/dl, MC...
Article
A trivalent measles-mumps-rubella (MMR) vaccine (MMR Berna) has been developed with a new mumps component, BBM-18, to replace a previously licensed MMR vaccine containing the Rubini mumps strain. Previous studies showed Rubini to confer insufficient long term protection against mumps infection. This study compared the immunogenicity and safety of M...
Article
The induction of tumor protective immunity against neuroblastoma remains a major challenge for active immunotherapy. Fractalkine is a unique Th1 CX3C chemokine known to induce adhesion and migration of leukocytes mediated by both, a membrane-bound and soluble form, respectively. Here, we tested the hypothesis that chemokine gene therapy with fracta...
Article
The disruption of self-tolerance against neuroblastoma is the ultimate goal of an effective DNA-vaccine. We demonstrate the induction of protective immunity against syngeneic murine NXS2 neuroblastoma in A/J mice following vaccination with tyrosine hydroxylase (TH)-derived antigens. Oral gene delivery was accomplished using an attenuated strain of...
Article
The tumor-associated antigen disialoganglioside-GD2 is expressed on neuroblastoma and melanoma and is an established target for passive immunotherapy. The aim of this study was to develop an active immunization strategy leading to the induction of a humoral anti-GD2 immune response. However, carbohydrates and glycolipids are T cell-independent anti...
Article
The diagnosis of Kawasaki disease is based on 6 clinical criteria, 5 of which must be fulfilled. The presence of uncommon symptoms in addition to the classic criteria can be as misleading as the lack of common ones. Here we report 2 infants with marked pulmonary symptoms in the course of Kawasaki disease who were initially diagnosed with pneumonia.
Article
To compare the immunogenicity and safety of a virosome-adjuvanted influenza vaccine (Inflexal V; Berna Biotech, Berne, Switzerland) and a split influenza vaccine (Fluarix; GlaxoSmithKline Biologicals, Rixensart, Belgium) in children. The subjects, 453 children ages 6 to 71 months, were stratified into primed and unprimed and age groups (6 to 35 and...
Article
In December 2000 and February 2001, two children with suspected meningococcal disease were admitted to our pediatric intensive unit. Their Glasgow Meningococcal Septicaemia Prognostic score was 12 points. General treatment including antibiotics, steroids in case of meningitis, and fluid replacement, was performed. Despite appropriate volume replace...
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Full-text available
Acute gastroenteritis is a major source of morbidity and mortality among young children in developed and developing countries. Enteropathogenic viruses are regarded as particularly relevant causative agents. Between February 2001 and January 2002, fecal specimens were obtained from German children admitted to hospital with acute gastroenteritis and...
Article
The induction of T-cell mediated immunity against neuroblastoma is a challenge due to poor immunogenicity of this malignancy. Here, we demonstrate the induction of protective immunity in a syngeneic murine neuroblastoma model following vaccination with minigenes comprising of three novel natural MHC class I ligands. First, after immunoprecipitation...
Article
Tumor directed cytotoxic therapy is one of the major challenges for the success of chemotherapy. In order to accomplish this goal in neuroblastoma, we rationally designed a prodrug of etoposide as substrate for tyrosine hydroxylase, a well established neuroblastoma associated enzyme. Here, we report synthesis and characterization of a 3,4 dihydroxy...
Article
Full-text available
Effective chemotherapy in neuroblastoma is limited by poor anti-tumor efficacy, systemic toxicity and the induction of drug resistance. Here, we provide further evidence that a hydrolytic activated prodrug design may overcome these problems. For this purpose, VP-16 was functionally blocked by a carbonate linker to generate two novel chemically stab...
Article
Full-text available
Effective therapy of high-risk leukemia with established cytotoxic drugs may be limited by poor antitumor efficacy, systemic toxicity, and the induction of drug resistance. Here, we provide the first evidence that hydrolytically activated prodrugs may overcome these problems. For this purpose, VP16 was functionally blocked by hydrolytically cleavab...
Article
Full-text available
The 93rd Annual Meeting of the American Association of Cancer Research was held in San Francisco, CA, USA. It was one of the largest conferences of its kind covering all aspects of cancer research with 6000 scientific presentations over a period of 5 days. The following meeting highlights are focused on a few selected developments in the field of t...
Article
The development of novel strategies for the treatment of malignancies by successful intervention in advanced stage disease is a major challenge in oncology. We tested the hypothesis that this can be achieved by the rational design of taxoid onium salts modified at C-7 and C-2' positions. The characterization of these molecules revealed a dramatical...
Article
Background: The development of novel strategies based on chemotherapy with prodrugs is still a challenge for physicians developing effective treatment of malignancies in advanced-stage disease. In this study, we tested the hypothesis that this can be achieved by a prodrug of paclitaxel if the C7 hydroxyl group is blocked by condensation with a solk...
Article
Two 4'-propylcarbonoxy derivatives (2,3) of etoposide (1), a topoisomerase II inhibitor, were synthesized and evaluated as potential prodrugs for anticancer therapy. Their activation via hydrolysis mechanisms was determined as a function of pH in buffer solutions, in human serum and in the presence of carboxyl ester hydrolase. Cytotoxicity was dete...
Article
Full-text available
The successful induction of T cell-mediated protective immunity against poorly immunogenic malignancies remains a major challenge for cancer immunotherapy. Here, we demonstrate that the induction of tumor-protective immunity by IL-12 in a murine neuroblastoma model depends entirely on the CXC chemokine IFN-gamma-inducible protein 10 (IP-10). This w...
Article
We present an 11-year-old girl with sensory and autonomic neurological dysfunction, and respiratory insufficiency caused by recurrent aspiration. The diagnosis of familial dysautonomia (FD) was confirmed by a missing axonal flare to histamine, miosis in response to conjunctival methacholine and homozygous polymorphic linked markers DS58(18) and DS1...
Article
The etiology of Kawasaki disease (KD) is still unknown. Therefore, the diagnosis relies on clinical criteria only. Although a specific therapy for KD is not available, coronary complications can be significantly reduced with the help of intravenous immunoglobulin (IVIG) therapy. It is not clear how IVIG interacts with the immune system. Previously,...
Article
The development of novel chemotherapy strategies based on prodrugs remains a major challenge for effective treatment of malignancies. We tested the hypothesis that this can be achieved by a prodrug of paclitaxel where one biologically active center, represented by the C7 hydroxyl group, was blocked by a dihydroxypropyl side chain which can be hydro...
Article
The disruption of self-tolerance against neuroblastoma is the ultimate goal of an effective DNA-vaccine. Here we demonstrate the induction of a protective immunity against syngeneic murine NXS2 neuroblastoma in A/J mice, following vaccination with tyrosine hydroxylase (TH) derived antigens. Oral gene delivery was accomplished using an attenuated st...
Article
To perform a comparative analysis of 1) intravenous Ig (IVIG)-bound Fab fragments from a patient with autoimmune thrombocytopenia that had progressed to systemic lupus erythematosus (SLE) and 2) IVIG-selected Fabs from an SLE patient without thrombocytopenia. IVIG preparations have been successfully used to treat certain cases of autoimmune thrombo...
Article
Full-text available
In search for a new therapeutic approach for metastasized renal cell carcinoma (RCC), we evaluated the cytotoxicity of a novel prodrug chemoimmunoconjugate with monoclonal antibody (mAb) 138H11 and the DNA-cleaving enediyne calicheamicin θ(I)1 (Camθ) in vitro and in vivo. Previously, mAb 138H11, produced against human renal γ-glutamyltransferase, s...