Gerhard Rechkemmer

Max Rubner-Institut | MRI

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ZusammenfassungFlüssigkeitsvolumen, Konzentrationen gelöster Stoffe und Produktion kurzkettiger Fettsäuren im Caecum und im oberen Colon des Meerschweinchens1Das durchschnittliche Gewicht des Caecuminhaltes beträgt beim anaesthesierten Meerschweinchen 4,7% des Körpergewichts.2Bei nicht betäubten Tieren ist der Caecuminhalt mit dem Inhalt des oberen...

Close, tightly orchestrated interactions between the intestinal epithelium and the mucosa-associated immune system are critical for normal intestinal absorptive and immunological functions. Recent data indicate that commensal intestinal microbiota represents a major modulator of intestinal homeostasis. This review analyzes the process of intestinal...

The aim of the study was to establish cell culture conditions for responsive guinea pig parietal cells. Parietal cells were isolated by a pronase/collagenase method, enriched by counterflow elutriation and cultured on plastic culture dishes in minimum essential medium. Precoating with gelatine or collagen increased adherence; optimum fetal calf ser...

The receptor for the inflammatory peptide C3a has scarcely been examined on human cells. This work demonstrates that human tumor-derived basophilic granulocytes express C3a receptors, and presents parts of the hitherto unknown C3a-signal transduction. When incubated with IL-3, these cells specifically liberated histamine on C3a stimulation. Indepen...

1. Animals were randomly assigned to three different treatment groups. (I) Control group maintained on a standard guinea pig diet, (II) a group obtaining a low-Na diet (0.02% Na w/w) and (III) a group fed the low-Na diet and in addition being treated with K-canrenoate prior to the perfusion experiment. 2. Proximal and distal colonic segments of ane...

Disposition of 1-[14C]naphthol was investigated in stripped gastric mucosal segments mounted in Ussing chambers. During 2-hr incubations, naphthol was glucuronidated (44-55% of added dose) and sulfated (7-15%). When naphthol was added to the luminal fluid at pH 7.0, conjugates were released with equal velocity to the luminal and to the vascular sid...

cRNA from a PCR-generated C5aR clone was prepared by in vitro transcription and microinjected into Xenopus laevis oocytes. Ligand-induced whole cell current could be detected after co-injection of cRNA for the C5aR with total RNA of the unstimulated U937 cell line, but not with either of the components injected alone. These data clearly demonstrate...

The ion transport mechanisms involved in electrogenic Cl secretion include a Cl exit step through the Cl channel at the apical membrane. In cystic fibrosis (CF), Cl secretion is impaired due to a missing Cl permeability of the apical membrane. Patch-clamp experiments have demonstrated that Cl channels are present in CF cells; however, the regulatio...

We characterized the anion channel responsible for the increase in apical membrane Cl secretion using a model salt-secreting epithelium, the T84 colonic cell line. The adenosine 3',5'-cyclic monophosphate (cAMP)-mediated secretagogues, prostaglandin E2, forskolin, and 8-bromo-cAMP, evoked activity of an outwardly rectifying Cl channel in previously...

Isolated mucosal sheets of guinea pig distal colon were studied in vitro in Ussing-type chambers, using a computer-controlled voltage clamp. A conductance of 8-12 mS/cm2 and spontaneous variations of the potential difference (Vt, -4 to +6 mV) and the short-circuit current (Isc, -1.6 to +1.5 mu eq X cm-2 X h-1) were observed. With use of a green fee...

A method for an analytical isolation of plasma membranes from columnar cells (colonocytes) of the proximal colon of the guinea pig is described. Isolation of the colonocytes was performed by a mild EDTA-chelation method. After homogenization, two subsequent sucrose gradient centrifugations (isokinetic and isopycnic) yielded a plasma-membrane fracti...

Hannover, Tierärztl. Hochsch., Habil.-Schr., 1989.