Geonildo Rodrigo Disner

Geonildo Rodrigo Disner
Karolinska Institutet | KI · Institute of Environmental Medicine - IMM

PhD; Toxicology
Affiliated to research

About

31
Publications
3,062
Reads
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227
Citations
Introduction
I am particularly interested in understanding the AhR-miRNA cross-talk in immunity using the zebrafish model. Also, study the epigenetic regulation by miRNAs in the absence of AhR (morpholino knockdown) and in response to the anti-inflammatory pre-drug TnP (peptide from Thalassophryne nattereri venom) in an LPS-challenged system. I also work with (eco)toxicology and immune response at the Immunoregulation Unit of the Laboratory of Applied Toxinology at Butantan Institute, São Paulo, Brazil.
Additional affiliations
July 2022 - April 2022
Instituto Butantan
Position
  • PostDoc Position
Description
  • Affiliated to the Center of Toxins, Immune Response, and Cell Signaling (CEPID/FAPESP) and Plataforma Zebrafish.
Education
April 2014 - March 2018
Universidade Federal do Paraná
Field of study
  • Environmental mutagenesis
April 2012 - March 2014
Universidade Federal do Paraná
Field of study
  • Environmental Mutagenesis and Animal Citogenetics
February 2008 - December 2011
Universidade do Oeste de Santa Catarina (UNOESC)
Field of study
  • Biological Science

Publications

Publications (31)
Article
Full-text available
Microplastics (MP) are emerging contaminants with the capacity to bind and transport hydrophobic organic compounds of environmental concern, such as polybrominated diphenyl ethers (PBDEs). The aim of this study was to investigate the ingestion of nylon (polyamide) MP alone and when associated with PBDEs and their effects on Chironomus sancticaroli...
Article
Full-text available
Melanoma is a type of tumor that originates from melanocytes. Irradiation of melanin with UVA and visible light can produce reactive oxygen species (ROS) such as singlet molecular oxygen (1O2). The objective of this study was to examine DNA damage in melanoma cells (B16‐F10) with different melanin contents, subjected to 1O2 generation. To this end,...
Chapter
Full-text available
A toxicologia é uma ciência muito antiga, já que as plantas e os animais desde os primórdios já apresentavam substâncias tóxicas em suas estruturas e o ser humano necessitava compreender suas ações como medidas de precaução. Com o surgimento da problemática gerada pela degradação exacerbada e poluição ambiental decorrente da revolução industrial do...
Article
Full-text available
Background The Natterin protein family was first discovered in the venom of the medically significant fish Thalassophryne nattereri , and over the last decade natterin-like genes have been identified in various organisms, notably performing immune-related functions. Previous findings support natterin-like genes as effector defense molecules able to...
Article
Full-text available
TmC4-47.2 is a toxin with myotoxic activity found in the venom of Thalassophryne maculosa, a venomous fish commonly found in Latin America whose envenomation produces an injury characterized by delayed neutrophil migration, production of major pro-inflammatory cytokines, and necrosis at the wound site, as well as a specific systemic immune response...
Article
The Butantan Institute is a pioneering Brazilian health sciences institution, which also houses a large science park with museums that contribute to ongoing science education for schools and the wider community. In recent years, as part of Butantan Institute’s Plataforma Zebrafish™, zebrafish embryos have been used for the dissemination of scientif...
Article
Full-text available
The aryl hydrocarbon receptor (AhR) is an environmentally responsive ligand-activated transcription factor, identified in the ‘70s for its toxic responses to halogenated polycyclic aromatic hydrocarbons, such as dioxin. Recently, AhR has been recognized as engaged in multiple physiological processes in health and diseases, particularly in the immun...
Article
Full-text available
Since the first record of the five founder members of the group of Natterin proteins in the venom of the medically significant fish Thalassophryne nattereri, new sequences have been identified in other species. In this work, we performed a detailed screening using available genome databases across a wide range of species to identify sequence member...
Article
Full-text available
Las abejas se encuentran entre los seres vivos más importantes para mantener y equilibrar los ecosistemas. Hay más de 20.000 especies que polinizan alrededor de 170.000 plantas en todo el mundo; por lo tanto, su conservación es una prioridad. Sólo en Brasil hay más de 1.860 especies de abejas, aproximadamente 300 de ellas sin aguijón. Sin embargo,...
Article
Full-text available
miRNAs regulate gene expression post-transcriptionally in various processes, e.g., immunity, development, and diseases. Since their experimental analysis is complex, in silico target prediction is important for directing investigations. TnP is a candidate peptide for anti-inflammatory therapy, first discovered in the venom of Thalassophryne nattere...
Article
Full-text available
Our recent data show the valuable potential of TnP for the development of a new and safe anti-inflammatory drug due to its ability to control the traffic and activation of leukocytes in response to inflammation. Although there is considerable knowledge surrounding the cellular mechanisms of TnP, less is known about the mechanistic molecular role of...
Article
Full-text available
The patented anti-inflammatory peptide TnP had its effectiveness recently confirmed in vivo in a murine model of multiple sclerosis and asthma. In this work, the safety of the TnP was evaluated in investigative toxicology tests using zebrafish (Danio rerio) as a model. We conducted the OECD #236 test to investigate effects of the TnP on the surviva...
Article
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p>A pandemia de COVID-19 levantou um antigo debate sobre a natureza dos vírus. Baseado em conceitos estritos, eles não têm metabolismo, homeostase interna, crescimento ou se replicam sem um hospedeiro, portanto, não poderiam estar vivos. A descoberta de vírus gigantes retoma o papel dos vírus na evolução, pois exibem maior complexidade e carregam u...
Article
The aquatic environment is the major recipient of wastes containing nanoparticles and other contaminants. Titanium dioxide nanoparticles (NPTiO2) are one of the most produced and used nanoparticle worldwide. This study investigated the toxicity of NPTiO2, as well as the toxicity interaction between NPTiO2 and lead (Pb), in response to genetic and b...
Article
Brazil is a global agricultural commodity producer and the largest consumer of pesticides. Intern pesticide use comprised 549,280 thousand tons in 2018. In the country, soybean, corn, and sugar cane are extensively produced, which are the most pesticides demanding crops. In the last years, the records of new pesticides were the highest in the histo...
Article
Full-text available
The oil spill off the Brazilian coast, which reached especially the Northeast in 2019, is known as the biggest disaster of its kind in the country's history. Accidents of this type are not new; however, this episode had enormous proportions with 11 states, 130 municipalities, and more than 1,000 locations affected by oil stains or residues. Conside...
Article
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Anualmente milhões de animais são usados em experimentos pelo mundo. A dor, o sofrimento e o sacrifício dos animais têm sido temas de intenso debate nos últimos anos. Existe uma demanda para a substituição de técnicas tradicionais usando animais indiscriminadamente por outros métodos. Estes baseiam-se na abordagem dos 3Rs (Reduction, Refinement e R...
Article
Genotoxic effects of nanomaterials (NMs) have been controversially reported in literature, and the mode of action (MoA) via DNA oxidation is cited as the main damage caused by them. Evidence of nano-silver as a crosslinker has been previously reported by the present research team in an in vivo fish genotoxicity study. Thus, aiming to confirm the ev...
Article
The textile dyeing industry is one of the main sectors contributing to environmental pollution, due to the generation of large amounts of wastewater loaded with dyes (ca. 2-50% of the initial amount of dyes used in the dye baths is lost), causing severe impacts on human health and the environment. In this context, an ecotoxicity testing battery was...
Article
Ibuprofen is a pharmaceutical drug widely used by the global population and it has been found in aquatic ecosystems in several countries. This study evaluated the effects of ibuprofen in environmental concentrations (0, 0.1, 1 and 10 μg/L) on the freshwaterspecies Rhamdia quelen exposed for 14 days. In the posterior kidney, ibuprofen increased glut...
Article
Nanotechnologies are at the center of societal interest, due to their broad spectrum of application in different industrial products. The current concern about nanomaterials (NMs) is the potential risks they carry for human health and the environment. Considering that NMs can reach bodies of water, there is a need for studying the toxic effects of...
Article
Full-text available
Polycyclic aromatic hydrocarbons are one of the most important organic pollutants in environmental studies. The aim of this study was to assess the naphthalene acute toxicity in two fish species, Astyanax lacustris (LLcust, 1875) and Geophagus brasiliensis (Quoy & Gaimard, 1824). The fish were exposed to naphthalene (0.005, 0.03, 0.3, and 3 mgL-1)...
Article
In-vivo effects of polybrominated diphenyl ethers (PBDEs) containing 3, 4 and 5 bromine atoms were tested on fourth-instar larvae of Chironomus sancticaroli (Diptera: Chironomidae) after 48 h of exposure, by measuring the activity of the acetyl cholinesterase, alpha and beta esterases and glutathione S-transferase. The PBDE congeners 2,2′,4-triBDE...
Article
Full-text available
Os hidrocarbonetos Aromáticos Policíclicos, como o Naftaleno, são considerados poluentes orgânicos prioritários em estudos ambientais. Alguns apresentam elevado potencial de toxicidade e podem ser precursores de efeitos mutagênicos e carcinogênicos. O teste de micronúcleo é uma ferramenta sensível na detecção de eventos clastogênicos e aneugênicos...
Article
Full-text available
Hantavirus cardiopulmonary syndrome (HCPS) is an infectious disease caused by hantaviruses of the family Bunyaviridae, and is transmitted by aerosols of excreta of infected rodents. The aim of the present study was to determine antibody levels to hantavirus in the population that lives at frontier of Brazil and Argentina. Participated of the study...
Article
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Os herbicidas a base de glifosato, como o Roundup®, são amplamente empregados em todo o mundo. Dados sobre a capacidade que esses compostos tóxicos têm de interferir no DNA, causando mutações, são importantes para o monitoramento de populações naturais. O objetivo deste trabalho foi avaliar a mutagenicidade do Roundup® por meio do teste de micronúc...
Article
Full-text available
A extração de DNA total a partir de bulbos capilares possibilita uma ampla diversidade de pesquisas e aplicações, além de permitir o envio de amostras a distância e ser utilizado na Medicina Forense, sendo um método amostral não invasivo. É necessária a busca por métodos de extração de DNA que sejam rápidos, de baixo custo, livres de contaminantes...

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Projects

Projects (2)
Project
Elucidate therapeutic properties of the novel peptide TnP using zebrafish as a model in collaboration with the Unit of Systems Toxicology, Karolinska Institut, Sweden (Funding FAPESP).
Project
This study aims to use Zebrafish as a model organism to investigate the role of the transcription factor AhR in regulating miRNA expression in response to TnP treatment and adjacent cellular and molecular mechanisms. Our hypothesis is that TnP activates the AhR receptor, which in turn undergoes cytosol translocation to the nucleus. Next, miRNAs can be produced leading to repression of inflammatory genes, induced by LPS treatment.