Geoffrey Prévot

Servier · Early Formulation and Drug Profiling

Regulating innate immunity is an emerging approach to improve cancer immunotherapy. Such regulation requires engaging myeloid cells by delivering immunomodulatory compounds to hematopoietic organs, including the spleen. Here we present a polymersome-based nanocarrier with splenic avidity and propensity for red pulp myeloid cell uptake. We character...

Macrophages are key inflammatory mediators in many pathological conditions, including cardiovascular disease (CVD) and cancer, the leading causes of morbidity and mortality worldwide. This makes macrophage burden a valuable diagnostic marker and several strategies to monitor these cells have been reported. However, such strategies are often high-pr...

Immunoparalysis is a compensatory and persistent anti-inflammatory response to trauma, sepsis or another serious insult, which increases the risk of opportunistic infections, morbidity and mortality. Here, we show that in cultured primary human monocytes, interleukin-4 (IL4) inhibits acute inflammation, while simultaneously inducing a long-lasting...

In the past 2 decades, research on atherosclerotic cardiovascular disease has uncovered inflammation to be a key driver of the pathophysiological process. A pressing need therefore exists to quantitatively and longitudinally probe inflammation, in preclinical models and in cardiovascular disease patients, ideally using non-invasive methods and at m...

Pharmacological and genetic studies over the past decade have established the follicle-stimulating hormone (FSH) as an actionable target for diseases affecting millions, namely osteoporosis, obesity, and Alzheimer's disease. Blocking FSH action prevents bone loss, fat gain and neurodegeneration in mice. We recently developed a first-in-class, human...

Purpose Though tolerance to kidney allografts has been consistently achieved using a mixed chimerism model, cardiac allografts remain resistant to tolerance induction. Lipoprotein-based nanobiologics loaded with a mammalian target of rapamycin inhibiting prodrug (mTOR inhibiting nanobiologics, mTORi-NBs) inhibit trained immunity and promote graft i...

Parkinson's disease (PD) is an age-related neurodegenerative disorder characterized by the loss of dopaminergic neurons in the substantia nigra, associated with the accumulation of misfolded α-synuclein and lysosomal impairment, two events deemed interconnected. Protein aggregation is linked to defects in degradation systems such as the autophagy-l...

Pharmacological and genetic studies over the past decade have established FSH as an actionable target for diseases affecting millions, notably osteoporosis, obesity and Alzheimer’s disease (AD). Blocking FSH action prevents bone loss, fat gain and AD–like features in mice. We recently developed a first–in–class, humanized, epitope–specific FSH bloc...

Atherosclerosis is at the onset of the cardiovascular diseases that are among the leading causes of death worldwide. Currently, high-risk plaques, also called vulnerable atheromatous plaques, remain often undiagnosed until the occurrence of severe complications, such as stroke or myocardial infarction. Molecular imaging agents that target high-risk...

The innate immune system plays a key role in atherosclerosis progression and the pathogenesis of cardiovascular disease. Trained immunity, an epigenetically regulated hyperresponsive state of myeloid cells, is a driving force underlying chronic inflammation in atherosclerosis. Therapeutically targeting innate trained immunity therefore may mature i...

Immunotherapy has firmly established itself as a compelling avenue for treating disease. Although many clinically approved immunotherapeutics engage the adaptive immune system, therapeutically targeting the innate immune system remains much less explored. Nanomedicine offers a compelling opportunity for innate immune system engagement, as many nano...

Immunotherapies controlling the adaptive immune system are firmly established, but regulating the innate immune system remains much less explored. The intrinsic interactions between nanoparticles and phagocytic myeloid cells make these materials especially suited for engaging the innate immune system. However, developing nanotherapeutics is an elab...

Trained immunity, a functional state of myeloid cells, has been proposed as a compelling immune-oncological target. Its efficient induction requires direct engagement of myeloid progenitors in the bone marrow. For this purpose, we developed a bone marrow-avid nanobiologic platform designed specifically to induce trained immunity. We established the...

Neurodegenerative diseases represent some of the greatest challenges for both basic science and clinical medicine. Due to their prevalence and the lack of known biochemical-based treatments, these complex pathologies result in an increasing societal cost. Increasing genetic and neuropathological evidence indicates that lysosomal impairment may be a...

Tailor-made NIR emitting dyes were designed as multimodal optical probes. These asymmetric amphiphilic compounds combine intense absorption in the visible region, NIR fluorescence emission, high two-photon absorption in the NIR (with maximum located around 1000 nm) as well as large Stokes’ shift values and second-harmonic generation ability. Thanks...

Most neurodegenerative disorders are characterized by deposits of misfolded proteins and neuronal degeneration in specific brain regions. Growing evidence indicates that lysosomal impairment plays a primary pathogenic role in these diseases, in particular the occurrence of increased lysosomal pH. Thus, therapeutic development aiming at restoring ly...

Les nanoémulsions huile dans eau (H/E) sont utilisées depuis plus de 50 ans en clinique humaine comme source de lipides en nutrition parentérale. Si cette dernière décennie a vu émerger la mise à profit de cette forme comme véhicule de substances actives lipophiles, l’utilisation des nanoémulsions comme vecteur d'agents thérapeutique ou diagnostiqu...

Parkinson’s disease (PD) is a neurodegenerative disorder characterized by the loss of dopaminergic neurons associated with an accumulation of α-synuclein aggregation. Growing evidence now indicates that lysosomal impairment may play a primary pathogenic role in this disease, in particular the occurrence of an abnormal lysosome pH. Previous works de...

The data presented in this article are related to the publication entitled “Iron oxide core oil-in-water nanoemulsion as tracer for atherosclerosis MPI and MRI imaging” [1]. Herein we describe the synthesis and the characteristics of the Superparamagnetic Iron Oxide Nanoparticles (SPION) loaded inside the nanoemulsions (NEs). Focus was set on obtai...

This article present data related to the publication entitled“Iron oxide core oil-in-water nanoemulsion as tracer for atherosclerosis MPI and MRI imaging” [1]. Herein we describe the engineering in the baculovirus-insect cell system and purification processes of the human scFv-Fc TEG4-2C antibody, specific of platelets within the atheroma plaque. F...

Purpose Effectiveness of nanomedicines in cancer therapy is currently limited by the short blood circulation time of nanocarriers. To overcome this limitation, we developed fluorescent nanoemulsions (NEs) decorated with 1% of PEGylated phospholipids to prolong blood circulation time and to improve intratumoral accumulation. Methods NEs were loaded...

Purpose: For early atherosclerosis imaging, magnetic oil-in-water nanoemulsion (NE) decorated with atheroma specific monoclonal antibody was designed for Magnetic Particle Imaging (MPI) and Magnetic Resonance Imaging (MRI). MPI is an emerging technique based on direct mapping of superparamagnetic nanoparticles which may advantageously complement MR...

Introduction : Multimodal imaging contrast agents are powerful probes which combine the complementary advantages of different imaging modalities and offer the prospect of improved preclinical applications. For this purpose, we engineered nanoemulsion (NE), submicron size range oily droplets. NE are widely used for human medical applications due to...

Introduction Atherosclerosis is a systemic disease affecting the arterials vessels walls and disturbing the blood flow. This complex, multifactorial and still not fully understood disease is difficult to correctly diagnose. Thus, atherosclerosis diagnosis could benefit from Magnetic Particle Imaging (MPI) an emerging preclinical imaging method base...

Atherosclerosis is a systemic disease affecting the arterials vessels walls in which lipid-triggered inflammation plays a critical role. Due to its complex, multifactorial and still not fully understood processes, atherosclerosis could benefit from new diagnosis approaches. Magnetic Particle Imaging (MPI) is an emerging preclinical imaging method w...

The aim of our study was to develop nanovesicular systems (nanoemulsions) for diagnosis purpose using a new imaging method : Magnetic Particle Imaging (MPI). MPI allows a direct quantitative detection of magnetic particles without suffering form the background tissue signal contrary to MRI [1, 2]. For this purpose we developed oil-in-water (o/w) na...