Gen Kaneko

• PhD
• Principal Scientist at Fermelanta Inc.

Dietary supplementation of plant herbs into aquafeed is recommended for intensive and sustainable aquaculture practices. This study has investigated the effect of Persicaria odorata (PO) leaf powder on growth, feed utilization, whole-body composition, hematology, and immune-related gene expression in Nile tilapia (Oreochromis niloticus). Fish (∼7.7...

In recent years, there has been a growing focus on using herbal extracts as immune enhancers for aquatic species, replacing antibiotics. In the present study, the effects of dietary supplementation of Hericium erinaceus extract (HE) on growth, feed utilization, hematology, expression of immunity-related genes, and immune responses in Nile tilapia i...

Simple Summary We exposed brown planthoppers (BPHs) Nilaparvata lugens to the entomopathogenic fungus Cordyceps javanica PSUC002 and observed morpho-histological changes from 0 to 120 h post-inoculation (pi). At 12 h pi, we observed the first filamentous fungi on the external integument and the first fungal conidia penetrated host planthopper cells...

This study aimed to explore the reproductive histology and oocyte differentiation of the longnose seahorse Hippocampus trimaculatus (Leach, 1814) in captivity. Five mature healthy females were histologically observed. The reproductive systems of the five specimens exhibited similar morphological characteristics with a pair of saccular creamy white...

The biodegradable polymer poly-β-hydroxybutyrate (PHB) is a promising carbon source for biological mitigation of nitrogen pollution, a significant problem in aquaculture that physical and chemical methods have not provided a comprehensive solution. Here we investigated the impact of PHB on the zero-water-change largemouth bass culture by 30- and 40...

The cartilage and bone structure has provided insightful knowledge about evolution and ecology of fish, which is an important component of biological oceanography. However, the whole-body bone staining is a lengthy and complicated process that typically takes five days to several months, and the improvement of the conventional method has been one o...

Microcystin-LR (MC-LR) is a cyanobacterial metabolite produced during cyanobacterial blooms and is toxic to aquatic animals, and the liver is the main targeted organ of MC-LR. To comprehensively understand the toxicity mechanism of chronic exposure to environmental levels of MC-LR on the liver of fish, juvenile Nile tilapia were exposed to 0 μg/L (...

Skeletal muscle is the mainly edible part of fish. Eicosapentaenoic acid (EPA) is a crucial nutrient for fish. This study investigated the effect of EPA on the muscle development of grass carp along with the potential molecular mechanisms in vivo and in vitro. Muscle cells treated with 50 μM EPA in vitro showed the elevated proliferation, and the e...

We investigated the digestive biology of two prevalent leiognathid species in Pranburi River estuary, Thailand: the decorated ponyfish (Nuchequula gerreoides) and the splendid polyfish (Eubleekeria splendens). A total of 632 samples collected from February to April and September to November 2017 were analysed using morphological and histological ap...

The present study aimed to investigate the effect of thermal stress on growth, feed utilization, coloration, hematology, liver histology, and critical thermal maximum (CTmax) in goldfish (Carassius auratus) cultured at three different acclimation temperatures including 27 °C, 30 °C, and 34 °C for 10 weeks. Goldfish were assigned randomly to tanks w...

Nuclear magnetic resonance (NMR) spectroscopy is a sensitive technique used to analyse the structure elucidation, dynamics, reaction state, and chemical environment of molecules. Abelmoschus esculentus and Lagenaria siceraria are edible plants used traditionally to treat jaundice, diabetes, weight loss, ulcer, hypertension, heart failure, skin dise...

The scalloped perchlet Ambassis nalua is one of the dominant fishes in the Estuarine Pranburi River, Thailand. It is suggested that this fish is in the secondary trophic level with a carnivorous nature. Studies on digestive system will help us further identify the niche of this species in the food web/food chain. The present study therefore aimed t...

Seafood mislabeling is a widespread problem that have produced a growing distrust of seafood industry. In this study, we examined the prevalence of mislabeling in fish samples from regional markets in the greater Houston area and close-by coastal communities. A total of 63 fish fillet samples were purchased, labeled, and stored at -20˚C in individu...

Metazoan 70 kDa heat shock protein (HSP70) genes have been classified into four lineages: cytosolic A (HSP70cA), cytosolic B (HSP70cB), endoplasmic reticulum (HSP70er), and mitochondria (HSP70m). Because previous studies have identified no HSP70cA genes in vertebrates, we hypothesized that this gene was lost on the evolutionary path to vertebrates....

The decorated ponyfish Nuchequula gerreoides and splendid ponyfish Eubleekeria splendens are two abundant leiognathid species in the Pranburi River Estuary, Thailand. However, information about their reproductive activity is still limited, preventing the implementation of effective conservative management. In this study, we investigated the sex rat...

The present study aimed to investigate the effect of thermal stress on growth, feed utilization, coloration, hematology, liver histology, and critical thermal maximum (CTmax) in goldfish ( Carassius auratus ) cultured at three different acclimation temperatures including 27˚C, 30˚C, and 34˚C for 10 weeks. Goldfish were assigned randomly to tanks wi...

The present study investigated the effects of chronic heat stress (HS) on the chemical composition, oxidative stability, muscle metabolism, and meat quality of Nile tilapia (Oreochromis niloticus). Compared with the control (26 °C), chronic HS (32 °C) lowered growth performance, the contents of whole-body lipid, muscle protein, and muscle lipid. Al...

Article Info Importance of the work: There is little known of the neurobiology of Allenbatrachus grunniens (Linnaeus 1758), an estuarine toadfish inhabiting mangrove areas of the Indo-West Pacific, including Thailand. Objectives: To identify the histomorphology of the brain and ultrastructure of the gonadotropic cells (GTH cells) in A. grunniens. M...

The market value of ornamental fish largely depends on the presence of carotenoids, which improve body coloration as well as growth and immunity. However, not many natural carotenoid sources have been tested as a dietary supplement for ornamental fish. The present study investigated the effect of dietary red pepper extracts (RPEs) supplementation o...

Spotted scat Scatophagus argus is a euryhaline species generally nursed in brackish water and shipped to freshwater ponds or sea cages of local farms in Thailand. To find the best practice for this process in terms of survival and cost, we conducted two culture experiments. In experiment 1, spotted scat fries (up to 6.72 ± 2.15 g) were divided into...

Task-free functional connectivity in animal models provides an experimental framework to examine connectivity phenomena under controlled conditions and allows for comparisons with data modalities collected under invasive or terminal procedures. Currently, animal acquisitions are performed with varying protocols and analyses that hamper result compa...

An 8-week feeding trial was conducted to determine the optimum dietary protein level for growth, feed utilisation, chemical composition and amino acid profile in juvenile spotted scat (Scatophagus argus). Five isoenergetic diets containing 31.70, 35.39, 40.71, 45.88 and 51.48% crude protein (T1-T5 respectively) were formulated and fed to triplicate...

The ambrosia beetle Xylosandrus compactus (Eichhoff, 1875) is a serious pest of economically important plants including Mitragyna speciosa, but its body structure and cellular composition remain uncharacterized. In this study, we described whole-body histological characteristics of female X. compactus. Our observation found two district regions in...

The objective of this study was to describe the structure and histochemistry of the systemic organs in the female marine water-strider Asclepios annandalei from Pranburi river estuary, Thailand. Results from this study revealed for the first time that the integumentary system of this species consisted of three layers including epicuticle, exocuticl...

Metazoan 70 kDa heat shock protein (HSP70) genes have been classified into four lineages: cytosolic A (HSP70cA), cytosolic B (HSP70cB), endoplasmic reticulum (HSP70er), and mitochondria (HSP70m). Because previous studies have identified no HSP70cA genes in vertebrates, we hypothesized that this gene has lost on the evolutionary path to vertebrates....

Water quality is closely related to the nutritional and sensory qualities of grass carp, which has the largest aquaculture production among freshwater fishes. The purpose of this study was to explore the effects of nitrite and nitrate on the growth performance, nutrition composition and muscle flavor-associated metabolites of grass carp through an...

Annotation of the 70 kDa heat shock proteins (Hsp70s) has been chaotic especially in invertebrates. In this study, we validated an emerging nomenclature of Hsp70s, which can be potentially applied to all metazoan Hsp70s, by conducting a genome-wide annotation of Caenorhabditis elegans Hsp70s. Using the phylogenetic annotation, the seven canonical C...

Reactive oxygen species (ROS) is known to increase muscle hardness, which has been of particular interest in food animals, but the detailed mechanism for the ROS‐induced changes remains unclear. To explore the responsible molecular pathway, we conducted a detailed gene/protein expression analysis on the crisp grass carp model using four groups fed...

Although the impacts of environmental problems on aquatic organisms have been broadly reported in Thailand, the literature has not covered the sand crab, Emerita taiwanesis Hsueh, 2015. In this study, we focused on the structure and health status of E. taiwanesis, an economically important crab species, living close to human activity areas in Sangc...

Light is a key environmental factor that is strongly related to fish activity and behavior. Exposure to certain wavelengths is known to affect growth performance and survival of teleost larvae and juveniles. Our study aimed to determine the effects of exposure to three light sources [red (710 nm), blue (453 nm) and green (510 nm)] on growth perform...

The stressful environment in Pranburi River estuary of Thailand severely impacts the aquatic ecosystem and may be causing reproductive alteration in the estuarine fishes living in this area, although there is little evidence of such deteriorated reproductive system. Thus, we aim to investigate the reproductive system, in particular the ovarian alte...

The growth performance of Scatophagus argus (25.53 ± 0.89 g) reared in Songkhla Lake, a brackish lagoon, was evaluated after feeding isonitrogenous and isocaloric diets containing hot-water extract from a seaweed, Sargassum sp., at 0, 0.25, 0.50, 0.75 or 1 g·kg⁻¹. Triplicate groups of fish were reared in 15 net cages (50 × 50 × 60 cm) at a density...

Black soldier fly (Hermetia illucens) larvae meal (BSFLM) is a promising animal feed ingredient because of its high protein content and sustainable nature in the aquaculture sector. For the potential application of this ingredient in goldfish culture, five isonitrogenous (42% of crude protein) and isolipidic (9% of crude fat) diets were prepared to...

Functional feed additives have been promising possibilities for improving fish immune function. The present study attempts to investigate the effect of dietary Hericium erinaceus (HE) supplementation on growth performance, hematology, disease resistance, and expression of genes related immune response against thermal challenge in Nile tilapia. Four...

Task-free functional connectivity in animal models provides an experimental framework to examine connectivity phenomena under controlled conditions and allows comparison with invasive or terminal procedures. To date, animal acquisitions are performed with varying protocols and analyses that hamper result comparison and integration. We introduce Sta...

Among legumes, broad bean (Vicia faba L.) has received particular attention due to its nutraceutical, functional and economic importance. The plant-derived microRNAs (miRNAs), as novel dietary functional components, are found to regulate the expression of endogenous mRNAs in vertebrates. To understand the role of broad bean miRNAs in the regulation...

The shortfin molly, Poecilia mexicana Steindachner, 1863, is an ovoviviparous fish. Unfortunately, the structural characteristics related to the digestive biology of this fish are still poorly known. In the current study, detailed characterization of the digestive system was conducted for P. mexicana from natural brackish water in Thailand, based o...

The traditional classification of stress-inducible 70 kDa heat shock protein (Hsp70) and heat shock cognate (Hsc70) requires a revision because of a recent finding that neither of them constitutes a monophyletic gene family. Here we inferred a phylogenetic relationship among Drosophila melanogaster Hsp70 family members. D. melanogaster Hsp70 family...

The kidney of fish contains numerous nephrons, each of which is divided into the renal corpuscle and renal tubules. This glomerular structure is the filtration unit of the nephron and is important for the kidney function, but it has been reported that the renal corpuscle was lost in at least four independent linages of fish (i.e., aglomerular kidne...

The utilization of sustainable and innovative raw materials to substitute for traditional fishmeal (FM) ingredients is required for the aquaculture sector. Sacha inchi meal (SIM), a by-product of sacha inchi oil, is one of the promising FM replacers. In the present study, four isonitrogenous and isolipidic (approximately 30 and 8%, respectively) di...

The anthropogenic and climate-driven rise in water temperature is expected to have an effect on the physiological functions of ectothermic species. In the present study, hybrid catfish were subjected to three different temperatures (27, 32, and 37 °C) for 50 days to examine the effect of long-term exposure to high temperatures on growth and physiol...

The 70 kDa heat shock proteins (HSP70s) and the constitutive members of the HSP70 family (heat shock cognates; HSC70s) play essential roles in various biological processes. The number of hsp70/hsc70 in the database is rapidly increasing because of their importance and the automatic annotation of newly sequenced genomes. However, accumulating eviden...

Functionally active components of faba bean (Vicia faba, FB) are known to improve the muscle texture and growth performance of grass carp (Ctenopharyngodon idellus), but this has not yet been proven by feeding trials using isonitrogenous and isoenergetic diets. Here, we formulated isonitrogenous and isoenergetic diets containing four types of FB ex...

The description of sperm morphology is fundamental in the reproductive biology of fishes, but this information is limited in the family Ambassidae. Our report hence focused on the ultrastructure and morphometric analysis of spermatozoa in a pelagic fish Ambassis nalua. All fish (n = 75) were obtained during January and March 2017 from the Estuarine...

Severe acute respiratory syndrome-Coronavirus-2 (SARS-CoV-2), a novel betacoronavirus, has surprised the world with its disease spread and mortality rate. SARS-CoV-2 is a positive-sense, enveloped RNA virus that can infect various organs of the body, potentially leading to multiple organ dysfunction and eventual death. While various medications hav...

The objective of this study was to determine the effects of partial replacement of fishmeal with sacha inchi seed (SIS) meal on growth performance, fatty acids composition, blood parameters, histological changes, and immune-related gene expression in Nile tilapia. Three iso-nitrogenous and iso-lipidic diets were formulated: a control diet without S...

Information on the reproductive biology of toadfish remains limited. In this study, we examined the structure and development of gonads in the grunting toadfish Allenbatrachus grunniens (Linnaeus, 1758) using morphological, histological and ultrastructural methods. The fish were collected from the Pranburi River Estuary, Thailand, during the annual...

Meat quality is closely associated with the chemical composition of skeletal muscle and is therefore influenced by the pre-mortem metabolic state of skeletal muscle tissue [...]

Koh Sichang area in Thailand is a sink for a wide variety of contaminants such as heavily polluted water from industry and oil spills. This situation may affect the health status of fish living in the area, but such information remains scarce. In this study, we evaluated the health status of java rabbitfish Siganus javus, an important marine fish i...

Physical analgesia has been used for the treatment of sports injuries. We previously developed a special patch (bumpy patch, BP) that adds pressure to the pain area, demonstrating its analgesic effects in various sports injuries. In this study, we further verified acute and chronic analgesic effects of the BP in 28 subjects (14–44 years of age, 15...

The morphology, histology and histochemistry of the digestive system and gut content of the wild-grunting toadfish (Allenbatrachus grunniens) from the estuary of Pranburi River, Thailand, is investigated. The digestive system of A. grunniens is elongated from the oral cavity to the intestine except for the stomach of a sac-shaped type. The length o...

The metazoan 70-kDa heat shock protein (HSP70) family contains several members localized in different subcellular compartments. The cytosolic members have been classified into inducible HSP70s and constitutive heat shock cognates (HSC70s), but their distinction and evolutionary relationship remain unclear because of occasional reports of “constitut...

We determined the complete mitochondrial genome of the Atlantic Gnomefish, Scombrops oculatus (Scombropidae). The total length of mitochondrial DNA (mtDNA) was 16,515 bp and included 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and one control region. The gene arrangement of S. oculatus was identical to those of three Ja...

Flavor and nutritional value are important qualities of freshwater fish products, but the key factors affecting these quality parameters remain unclear. In this study, four typical aquaculture modes, including the commercial feed treatment (control), faba bean treatment (FBT), grass powder treatment (GPT), and waving water treatment with commercial...

The first author recognized an error within the manuscript and requested for a correction to be made for Figure 1(A) on page 54. The upper image of Figure 1(A) was a repetition of another manuscript that was published previously. The use of the same figure for another publication elsewhere is prohibited and therefore the new figure is printed below...

The Indo-Pacific seahorse, Hippocampus barbouri, is one of the most important marine fish and a potential target of aquaculture, but basic biological information on this fish is largely missing. In this study, we described the hepatocyte ultrastructure and the ontogeny of the liver in H. barbouri during the 1st and the 35th day after birth (DAB). T...

Natural oxidant faba beans (FB) improve the muscle textural quality of grass carp (Ctenopharyngodon idellus). This study further explored the role and spatiotemporal dynamics of ROS during the 120-day process of texture quality change in grass carp fed with four feeds including commercial feed, FB only, FB and antioxidants (vitamin C and vitamin E)...

A 10-week feeding trial was conducted to examine the effect of dietary sacha inchi meal (SIM) as a soybean meal (SBM) replacer on growth performance, feed utilization, whole-body fatty acid composition, blood biochemistry, and histological changes in red hybrid tilapia, Oreochromis niloticus × O. mossambicus. Five isonitrogenous and isolipidic diet...

Precision medicine is a medical approach to administer patients with a tailored dose of treatment by taking into consideration a person’s variability in genes, environment, and lifestyles. The accumulation of omics big sequence data led to the development of various genetic databases on which clinical stratification of high-risk populations may be...

In the present study, we describe the spermatogenesis of Allenbatrachus grunniens, a benthic toad-fish from Estuarine Pranburi River, Thailand, at the ultrastructural level. According to the nuclear and cyto-plasmic characteristics, A. grunniens spermatogenic cells are classified into five stages. The spermatogonium contains the prominent nucleus a...

Knowledge of the reproductive biology of the toadfish is still limited. The present study therefore aimed to provide basic knowledge about ovarian histology in the mangrove toadfish Batrachomoeus trispinosus, a potential aquaculture species in Thailand. To this end, we performed histological analysis of the ovaries (n = 20, all females, total lengt...

The sesarmid crab Episesarma singaporense is an economically important crab in Thailand. In this study, we associated the integrated classification of oocyte development with ovarian morphology and the gonadosomatic index (GSI). All specimens were collected in October 2018 and January – February 2019. The germinative centers of the ovary contained...

The fish insulin/insulin-like growth factor (IGF) pathway has weak control over carbohydrate metabolism. To understand the molecular basis for the metabolic diversity, we characterized the forkhead box transcription factor O1A (FoxO1A), a downstream target of the insulin/IGF pathway, in torafugu Takifugu rubripes. The cloned torafugu FoxO1A cDNA co...

Histopathological examination is a widely acknowledged technique to assess the reproductive health of aquatic organisms, but it has never been applied to the tunicate Styela plicata, a potential indicator species of water quality. In this study, we examined the oocyte differentiation of S. plicata obtained from the eastern coast of the Gulf of Thai...

The zebra-snout seahorse, Hippocampus barbouri, is an economically important marine fish and a potential candidate for aquaculture in Thailand. However, the reproductive ultrastructure of this seahorse is still poorly known. Transmission Electron Microscope (TEM) was used to characterize cellular morphology and ultrastructure of gametogenic stages...

Reproductive characteristics on the snake eel Pisodonophis boro, a commercially important and high-value food source species in Thailand, have never been reported. We determined the body size distribution and gonadal structure of P. boro during the reproductive cycle. Healthy specimens were collected by local Pranburi River estuarine fishermen duri...

Ovoviviparous poeciliid fishes have been relatively well studied in the unique reproductive strategy, but their osmoregulatory system largely remains unknown. In this study, we conducted a short-term (7 days) lab experiment to investigate the effect of different salinity levels from 0 (freshwater) to 50 ppt (mesosaline) on the number of chloride ce...

The central nervous system (CNS) of Teleostei is a complex system of self-governance and its morphology is reflected in the physiological and reproductive behaviors. The Indo-Pacific seahorse, Hippocampus barbouri Jordan & Richardson, 1908, is a new candidate species for aquaculture in Thailand. In this study, we investigated the brain morphology o...

Crisp grass carp products from China are becoming more prevalent in the worldwide fish market because muscle hardness is the primary desirable characteristic for consumer satisfaction of fish fillet products. Unfortunately, current instrumental methods to evaluate muscle hardness are expensive, time-consuming, and wasteful. This study sought to dev...

It is well-known that pollutants affect aquatic ecosystems; however, there is little information on fish reproductive health as an indicator of aquatic pollution. This study reports the oogenesis and ovarian health problems in important fishes from different habitats potentially affected by pollution. Nine fish species caught in 2016 to 2017 were s...

Considering the excessive lipid accumulation status caused by the increased dietary lipid intake in farmed fish, this study aimed to investigate the systemic effect of dietary lipid levels and α-lipoic acid supplementation on nutritional metabolism in zebrafish. A total of 540 male zebrafish (0.17 g) were fed with normal (CT) and high lipid level (...

The 70-kDa heat shock protein (HSP70) family contains several isoforms localized in different subcellular compartments. The cytosolic isoforms have been classified into stress-inducible HSP70s and constitutive heat shock cognates (HSC70s), but occasional reports of “constitutive HSP70s” and the lack of cross-phylum comparisons have been a source of...

Several recent studies have reported that systemic corticosteroids are effective against COVID‐19¹. COVID‐19 mainly affects the respiratory system, with minor damage to other organs. This article is protected by copyright. All rights reserved.

Khlong Saen Saep in Bangkok is a polluted canal whose water quality fails to meet standard criteria in many areas. We hence assessed the effects of pollution on the striped catfish Pangasianodon hypophthalmus (Sauvage, 1878) commonly found in polluted areas of the canal. Fish were sampled from two stations in Khlong Saen Saep: Masjid Kamalulislam a...

Faba bean ( Vicia faba , FB) is known to improve the texture of fish meat but retards growth possibly by inducing hemolysis, hepatopancreas damage, and metabolic disorder. In this study, we used ultrasonic processing to isolate four FB extracts (water extract, alcohol extract, proteins and residues) and examined their beneficial and detrimental eff...