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Geetanjali Chawla

Geetanjali Chawla
Shiv Nadar Institute of Eminence · Department of Life Sciences

PhD

About

42
Publications
10,298
Reads
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1,709
Citations
Additional affiliations
January 2018 - December 2022
Regional Centre for Biotechnology
Position
  • DBT-Wellcome Intermediate Fellow
July 2017 - July 2017
Indiana University Bloomington
Position
  • Research Associate
July 2012 - June 2017
Indiana University Bloomington
Position
  • Scientist

Publications

Publications (42)
Article
Full-text available
Dietary restriction (DR) extends healthy lifespan in diverse species. Age and nutrient-related changes in the abundance of microRNAs (miRNAs) and their processing factors have been linked to organismal longevity. However, the mechanisms by which they modulate lifespan and the tissue-specific role of miRNA-mediated networks in DR-dependent enhanceme...
Article
Full-text available
Evaluation of nutritionally enhanced biofortified dietary interventions that increase lifespan may uncover cost-effective and sustainable approaches for treatment of age-related morbidities and increasing healthy life expectancy. In this study, we report that anthocyanin rich, high yielding crossbred blue wheat prolongs lifespan of Drosophila melan...
Article
Full-text available
Differential processing is a hallmark of clustered microRNAs (miRNAs) and the role of position and order of miRNAs in a cluster together with the contribution of stem-base and terminal loops has not been explored extensively within the context of a polycistronic transcript. To elucidate the structural attributes of a polycistronic transcript that c...
Article
Full-text available
Exosomes are a class of extracellular vesicles that play a role in intercellular signaling under diverse contexts. Here, we describe a protocol that has been optimized for the isolation and characterization of exosomes from a Drosophila melanogaster cell line using size exclusion chromatography (SEC). The specific focus of this protocol was to exam...
Preprint
Full-text available
Drosophila melanogaster larval development relies on a specialized metabolic state that utilizes carbohydrates and other dietary nutrients to promote rapid growth. One unique feature of the larval metabolic program is that Lactate Dehydrogenase (Ldh) activity is highly elevated during this growth phase when compared to other stages of the fly life...
Preprint
Full-text available
Dietary restriction (DR) delays aging and neurodegeneration, but the mechanisms behind this remain to be elucidated. We identified genetic polymorphisms in mustard (mtd), the fly homolog of Oxidation resistance 1 (OXR1), which influenced lifespan and mtd expression in neurons in response to DR regulated by the transcription factor Traffic jam (TJ)....
Article
Dietary restriction (DR) has long been viewed as the most robust nongenetic means to extend lifespan and healthspan. Many aging-associated mechanisms are nutrient responsive, but despite the ubiquitous functions of these pathways, the benefits of DR often vary among individuals and even among tissues within an individual, challenging the aging rese...
Article
The functional and structural versatility of Ribonucleic acids (RNAs) makes them ideal candidates for overcoming the limitations imposed by small molecule-based drugs. Hence, RNA-based biopharmaceuticals such as messenger RNA (mRNA) vaccines, antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), microRNA mimics, anti-miRNA oligonucleo...
Preprint
Full-text available
Dietary restriction (DR) extends healthy lifespan in diverse species. Age and nutrient-related changes in the abundance of microRNAs (miRNAs) and their processing factors have been linked to organismal longevity. However, the mechanisms by which they modulate lifespan and the tissue-specific role of miRNA mediated networks in DR dependent enhanceme...
Article
Full-text available
Cell growth and/or proliferation may require the reprogramming of metabolic pathways, whereby a switch from oxidative to glycolytic metabolism diverts glycolytic intermediates towards anabolic pathways. Herein, we identify a novel role for TRIM32 in the maintenance of glycolytic flux mediated by biochemical interactions with the glycolytic enzymes...
Article
Full-text available
Cell growth and/or proliferation may require the reprogramming of metabolic pathways, whereby a switch from oxidative to glycolytic metabolism diverts glycolytic intermediates towards anabolic pathways. Herein, we identify a novel role for TRIM32 in the maintenance of glycolytic flux mediated by biochemical interactions with the glycolytic enzymes...
Article
Full-text available
Cell growth and/or proliferation may require the reprogramming of metabolic pathways, whereby a switch from oxidative to glycolytic metabolism diverts glycolytic intermediates towards anabolic pathways. Herein, we identify a novel role for TRIM32 in the maintenance of glycolytic flux mediated by biochemical interactions with the glycolytic enzymes...
Article
Full-text available
During Drosophila melanogaster metamorphosis, arrested immature neurons born during larval development differentiate into their functional adult form. This differentiation coincides with the downregulation of two zinc-finger transcription factors, Chronologically Inappropriate Morphogenesis (Chinmo) and the Z3 isoform of Broad (Br-Z3). Here, we sho...
Chapter
MicroRNAs (miRNAs) belong to a class of small non-coding endogenous RNAs that regulate gene expression at the post-transcriptional level. These small RNAs recognize sequences within 3′ untranslated regions of target mRNAs in complexes referred to as a miRNA-induced silencing complex (miRISC). Drosophila melanogaster has served as an indispensable m...
Article
Full-text available
Macrophage-mediated phagocytosis and cytokine production represent the front lines of resistance to bacterial invaders. A key feature of this pro-inflammatory response in mammals is the complex remodeling of cellular metabolism towards aerobic glycolysis. Although, the function of bactericidal macrophages is highly conserved, the metabolic remodeli...
Article
Full-text available
The dramatic growth that occurs during Drosophila larval development requires rapid conversion of nutrients into biomass. Many larval tissues respond to these biosynthetic demands by increasing carbohydrate metabolism and lactate dehydrogenase (LDH) activity. The resulting metabolic program is ideally suited for synthesis of macromolecules and mimi...
Article
Full-text available
Significance Oncometabolites are small molecules that promote tumor formation and growth. L-2-hydroxyglutarate (L-2HG) is a putative oncometabolite that is associated with gliomas and renal cell carcinomas, as well as a severe neurometabolic disorder known as L-2-hydroxyglutaric aciduria. However, despite that L-2HG is commonly considered a metabol...
Chapter
MicroRNAs are short noncoding, ~22-nucleotide RNAs that regulate protein abundance. The growth in our understanding of this class of RNAs has been rapid since their discovery just over a decade ago. We now appreciate that miRNAs are deeply embedded within the genetic networks that control basic features of metazoan cells including their identity, m...
Article
Full-text available
Messenger RNAs (mRNAs) often contain binding sites for multiple, different microRNAs (miRNAs). However, the biological significance of this feature is unclear, since such co-targeting miRNAs could function coordinately, independently, or redundantly with one another. Here, we show that two co-transcribed Drosophila miRNAs, let-7 and miR-125, non-re...
Article
Full-text available
Adenosine deaminases acting on RNAs (ADARs) convert adenosine residues to inosines in primary microRNA (pri-miRNA) transcripts to alter the structural conformation of these precursors and the subsequent functions of the encoded microRNAs (miRNAs). Here we show that RNA editing by Drosophila ADAR modulates the expression of three co-transcribed miRN...
Article
Significance Understanding the neuronal functions of diverse RNA pathways will lead to treatments of human neurological diseases that are caused by perturbations in RNA metabolism. Two proteins, Drosha and Pasha/DGCR8, play important roles in neurons, where they are responsible for the biogenesis of many microRNAs. Here, we show that Pasha/DGCR8 al...
Article
MicroRNAs (miRNAs) ensure progression through development by synchronizing cell fate transitions in response to environmental cues. These cues are mediated at least in part by steroid hormones. Emerging evidence indicates that miRNAs are also components of additional systemic signaling pathways, including insulin, stress, immune, and circadian path...
Chapter
MicroRNAs belong to a class of 20–25 nucleotide noncoding RNAs that posttranscriptionally regulate gene expression in a sequence-specific manner. Members of the microRNA family play roles in the normal physiology and development of diverse organisms. In this review, we focus on the recent progress made in understanding the roles of microRNAs in Dro...
Article
Full-text available
Steroid hormones and their nuclear receptors drive developmental transitions in diverse organisms, including mammals. In this study, we show that the Drosophila steroid hormone 20-hydroxyecdysone (20E) and its nuclear receptor directly activate transcription of the evolutionarily conserved let-7-complex (let-7-C) locus, which encodes the co-transcr...
Article
Full-text available
Postsynaptic density protein 95 (PSD-95) is essential for synaptic maturation and plasticity. Although its synaptic regulation has been widely studied, the control of PSD-95 cellular expression is not understood. We found that Psd-95 was controlled post-transcriptionally during neural development. Psd-95 was transcribed early in mouse embryonic bra...
Article
Micro-ribonucleic acids (miRNAs) are small (21-24 nucleotide), endogenously expressed, noncoding RNAs that have emerged as important posttranscriptional regulators of gene expression. MiRNAs have been identified and cloned from diverse eukaryotic organisms where they have been shown to control important physiological and developmental processes suc...
Article
Full-text available
CaV1.2 calcium channels play roles in diverse cellular processes such as gene regulation, muscle contraction, and membrane excitation and are diversified in their activity through extensive alternative splicing of the CaV1.2 mRNA. The mutually exclusive exons 8a and 8 encode alternate forms of transmembrane segment 6 (IS6) in channel domain 1. The...
Article
Full-text available
Sam68 (Src-associated in mitosis, 68 kDa) is a KH domain RNA binding protein implicated in a variety of cellular processes, including alternative pre-mRNA splicing, but its functions are not well understood. Using RNA interference knockdown of Sam68 expression and splicing-sensitive microarrays, we identified a set of alternative exons whose splici...
Article
Full-text available
Many metazoan gene transcripts exhibit neuron-specific splicing patterns, but the developmental control of these splicing events is poorly understood. We show that the splicing of a large group of exons is reprogrammed during neuronal development by a switch in expression between two highly similar polypyrimidine tract-binding proteins, PTB and nPT...
Article
Full-text available
Alternative pre-mRNA splicing determines many changes in gene expression during development. Two regulators known to control splicing patterns during neuron and muscle differentiation are the polypyrimidine tract-binding protein (PTB) and its neuronal homolog nPTB. These proteins repress certain exons in early myoblasts, but upon differentiation of...
Article
Full-text available
Saccharomyces cerevisiaePRP17 (CDC40) encodes a second‐step pre‐mRNA splicing factor with a role in cell division. The functions of Prp17 in specific cell cycle transitions were examined using temperature‐sensitive alleles in arrest/release experiments. We find that G1/S and G2/M transitions depend on Prp17. G1‐synchronized prp17::LEU2 cells arrest...
Article
Full-text available
In Saccharomyces cerevisiae, Prp17p is required for the efficient completion of the second step of pre-mRNA splicing. The function and interacting factors for this protein have not been elucidated. We have performed a mutational analysis of yPrp17p to identify protein domains critical for function. A series of deletions were made throughout the reg...

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