Gayandi Ekanayake

Estonian University of Life Sciences | EMU · Faculty of Veterinary Medicine

Purpose: Stem-End Rot (SER) is one of the most frequently found mango postharvest diseases in many countries including Sri Lanka. Availability of a significantly detailed study on morphological and molecular characterization of the SER associated fungal species is comparatively low. The present study has been carried out considering the above fact....

Increasing antimicrobial resistance (AMR) challenges conventional antibiotics, prompting the search for alternatives. Extracellular vesicles (EVs) from pasteurised cattle milk offer promise, due to their unique properties. This study investigates their efficacy against five pathogenic bacteria, including Staphylococcus aureus ATCC 25923, aiming to...

Background Bacillus cereus is commonly found in many foods and known for causing foodborne illness. Due to their spore-forming and biofilm-forming nature and contamination capability these bacteria are presenting a large challenge to the food industry. The bacterium has become increasingly resistant to multiple drugs necessitating novel preventive...

Inhibitory Effects of Chlorella vulgaris Algasomes on Listeria species Growth and Biofilm Eradication Dulmini Nanayakkara Sapugahawatte1Ɨ, Kasun Godakumara1 Ɨ, Gayandi Ekanayake1, Deep Bhattacharya1, Getnet Balcha Midekessa1,2, Mati Roasto3, Renu Geetha Bai4, Timo Kikas4, Aneta Andronowska5, Alireza Fazeli1,2,6* 1. Institute of Veterinary Medicin...

Introduction Extracellular vesicles (EVs) facilitate inter and intra-species/kingdom communication through biomolecule transfer, including proteins and small RNAs. Plant-derived EVs, a hot topic in the field, hold immense capability both as a potential biomarker to study plant physiology and as a biomaterial that can be mass-produced to be used in...

Effective suppression of Staphylococcus aureus ATCC 25923 growth through periodic dosing of pasteurized milk-derived extracellular vesicle Dulmini Nanayakkara Sapugahawtte1, Kasun Godakumara1, Mihkel Mäesaar2, Gayandi Ekanayake1, Getnet Balcha Midekessa1,3, Madhusha Prasadini1, Suranga Kodithuwakku1,4, Mati Roasto2, Aneta Andronowska5, Alireza Faze...

Background/ hypothesis Bovine mastitis, a global dairy cattle disease, impacts animal health, milk quality and public health due to its zoonotic potential. Staphylococcus aureus, Streptococcus agalactiae (GBS), and Escherichia coli are the key mastitis-causing pathogens. This study examined whey and algae spent media-derived extracellular vesicles...

Extracellular vesicles (EVs), play a crucial role in mammalian cell-to-cell communication, by transporting components such as proteins and small RNAs. Similarly, plants are also believed to be producing EVs under various circumstances. Herein, we attempted to purify and characterize vesicle-like structures separately, from initial sampling solution...

This is the lab hand book of the EV training school- 2022. Contributors; Kasun Godakumara , Gayandi Ekanayaka, Qurat Ul Ain , Madhusha Prasadani, Getnet Midekessa , Omidreza Mousavi, Keerthi Dissanayaka , Subhashini Muhandiram, Mehedi Hasan, Johanna Piibor, Chanaka Premathilaka https://dspace.emu.ee/handle/10492/7997

Both native and non‐native bamboo populations can expand their distributions in a rather irregular manner, inflicting changes in native vegetation with potential consequences for litter‐dwelling arthropod communities. To our knowledge, no studies have been undertaken to explore the impacts of bamboo spread on litter‐dwelling arthropods. This study...

Tea [Camellia sinensis (L.) O. Kuntze], the second most consumed beverage in the world, is a rich source of polyphenolic compounds. The characteristic properties of tea depend on the biochemical compounds present in tea leaf. Hybridization of tea aims at developing new cultivars with more favorable levels of biochemicals for commercial exploitation...

Karutha Colomban is one of the most delightful mango varieties popular among Sri Lankan consumers. A significant postharvest loss of mango takes place every season due to diseases including stem-end rot (SER), which is caused by a group of endophytic fungal pathogens. In this research, in vitro antifungal efficacy of different concentrations of ess...

number 2-01 In vitro antifungal activity of Cymbopogon citratus (lemongrass) and Syzygium aromaticum (clove) oils on the Stem-end rot pathogen-Lasiodiplodia theobromae from mango Stem-end rot (SER) disease causes considerable losses to mango fruits throughout the supply chain. The aim of this research was to evaluate the antifungal potential of lem...

Stem-end rot (SER) disease causes considerable losses to mango fruits throughout the supply chain. The aim of this research was to evaluate the antifungal potential of lemongrass and clove oils against Lasiodiplodia theobromae which is a frequent SER causative agent of mango fruits (cv. Karutha Colomban). Under In vitro conditions, lemongrass and c...

Chundikulam sanctuary is a reserve which is located in the Northern Province, in Jaffna peninsula. This reserve is situated in the dry zone of Sri Lanka and the area consists of mangroves along the shores and scrub lands in the sandy areas. The Jaffna peninsula, including Chundikulam, faced an ecological destruction due to the Sri Lankan civil war...